In the present study,the antioxidant and anti-human liver cancer(HepG2)cells effects of bioactive peptides from cowhide collagen(BPCC)were evaluated.BPCC exhibited significant scavenging effect on l,1-diphenyl-2-picry...In the present study,the antioxidant and anti-human liver cancer(HepG2)cells effects of bioactive peptides from cowhide collagen(BPCC)were evaluated.BPCC exhibited significant scavenging effect on l,1-diphenyl-2-picrylhydrazyl(DPPH)radicals((60.09±3.51)%),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radicals((77.40±3.10)%)and hydroxyl radicals((56.00±2.30)%)as well as strong reducing power(0.320士0.025).Meanwhile,BPCC effectively protected biomacromolecules including proteins,lipids and DNA from oxidative damage induced by Cu^(2+)H_(2)O_(2)and 2,2'-azobis(2-methylpropionamidine)dihydrochloride(AAPH).Moreover,BPCC significantly inhibited cell viability of HepG2 cells in a dose-dependent manner with an estimated IC_(50)of 7.61 mg/mL.The results of 4',6-diamidino-2-phenylindole(DAPI)and acridine:orange/ethidium bromide(AO/EB)staining demonstrated the apoptotic morphological changes and cell mediated death in BPCC treated HepG2 cells.In addition,BPCC induced decrease of mitochondrial membrane potential(MMP)in HepG2 cells.Therefore,the present finding proved that BPCC encompasses significant antioxidant activity and anticancer property on HepG2 cells and can be used as alternative food antioxidants for cancer prevention benefits.展开更多
This article discusses the history of animal glue(gelatin)used in medical practice in China.In ancient times,gelatin was widely made from cowhide and has been found in excavations and wall paintings older than 2000 ye...This article discusses the history of animal glue(gelatin)used in medical practice in China.In ancient times,gelatin was widely made from cowhide and has been found in excavations and wall paintings older than 2000 years in Egypt,Greece,and China.However,it has been used medicinally almost exclusively in China and other East Asian countries influenced by Chinese medicine.Since 11th century,donkey-hide has gradually been replaced with cowhide in medicinal gelatin.Currently,donkey-hide gelatin is widely used medicinally in China.During the Edo period,medicinal gelatin was imported to Japan from China.Owing to the distaste for eating animal flesh in traditional Japanese culture,the production of gelatin and its medicinal use is not widespread in Japan.展开更多
基金supported by Shandong Provincial Natural Science Foundation,China(ZR2014CQ002)SDUT and Zibo City Integration Development Project(2017ZBXC004)。
文摘In the present study,the antioxidant and anti-human liver cancer(HepG2)cells effects of bioactive peptides from cowhide collagen(BPCC)were evaluated.BPCC exhibited significant scavenging effect on l,1-diphenyl-2-picrylhydrazyl(DPPH)radicals((60.09±3.51)%),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radicals((77.40±3.10)%)and hydroxyl radicals((56.00±2.30)%)as well as strong reducing power(0.320士0.025).Meanwhile,BPCC effectively protected biomacromolecules including proteins,lipids and DNA from oxidative damage induced by Cu^(2+)H_(2)O_(2)and 2,2'-azobis(2-methylpropionamidine)dihydrochloride(AAPH).Moreover,BPCC significantly inhibited cell viability of HepG2 cells in a dose-dependent manner with an estimated IC_(50)of 7.61 mg/mL.The results of 4',6-diamidino-2-phenylindole(DAPI)and acridine:orange/ethidium bromide(AO/EB)staining demonstrated the apoptotic morphological changes and cell mediated death in BPCC treated HepG2 cells.In addition,BPCC induced decrease of mitochondrial membrane potential(MMP)in HepG2 cells.Therefore,the present finding proved that BPCC encompasses significant antioxidant activity and anticancer property on HepG2 cells and can be used as alternative food antioxidants for cancer prevention benefits.
基金financed by a grant from the Zhejiang Gongshang University,the Zhejiang Province"Foreign Language and Literature"Top Disciplines(A Class)Construction High-level Project(No.2020YLZS16)。
文摘This article discusses the history of animal glue(gelatin)used in medical practice in China.In ancient times,gelatin was widely made from cowhide and has been found in excavations and wall paintings older than 2000 years in Egypt,Greece,and China.However,it has been used medicinally almost exclusively in China and other East Asian countries influenced by Chinese medicine.Since 11th century,donkey-hide has gradually been replaced with cowhide in medicinal gelatin.Currently,donkey-hide gelatin is widely used medicinally in China.During the Edo period,medicinal gelatin was imported to Japan from China.Owing to the distaste for eating animal flesh in traditional Japanese culture,the production of gelatin and its medicinal use is not widespread in Japan.
