目的:探讨CRBN基因在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其与MAPK信号通路的关系。方法:通过GEO、GEPIA、TCGA等数据库分析来那度胺作用靶点CRBN在DLBCL中的功能及其与相关信号通路之间的关系,并收集2...目的:探讨CRBN基因在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其与MAPK信号通路的关系。方法:通过GEO、GEPIA、TCGA等数据库分析来那度胺作用靶点CRBN在DLBCL中的功能及其与相关信号通路之间的关系,并收集2016年01月至2020年10月确诊为DLBCL,且经过来那度胺治疗的病例57例,免疫组化染色(IHC)和多重免疫荧光检测CRBN、MAPK的蛋白表达,进行统计分析。结果:通过生物信息学数据分析发现,CRBN通过MAPK信号通路影响细胞增殖和免疫微环境。CRBN和MAPK在DLBCL中高表达,且CRBN的表达与MAPK表达呈正相关(P<0.05);CRBN和MAPK高表达患者容易从来那度胺治疗中获益(P<0.05)。结论:CRBN可能通过MAPK信号通路对DLBCL的发生发展起作用,CRBN和MAPK蛋白的表达可以作为来那度胺疗效的评估参考依据。展开更多
To compare the merits of Ni and Cu,the mechanical and tribological properties of CrBN coatings modified by Ni or Cu incorporation were studied.The results demonstrated that the CrBN–Cu coatings presented a lower fric...To compare the merits of Ni and Cu,the mechanical and tribological properties of CrBN coatings modified by Ni or Cu incorporation were studied.The results demonstrated that the CrBN–Cu coatings presented a lower friction coefficient than CrBN and CrBN–Ni coatings owing to the improved lubrication effect of the CuO layer originating from the tribochemical reaction.However,the hardness decline due to Cu incorporation was much greater than that of Ni incorporation.Thus,the CrBN–Cu coatings exhibited a higher wear rate than the CrBN coating.In contrast,the plastic deformation enhancement induced by Ni incorporation exceeded the hardness decline.Therefore,the wear of CrBN–Ni coatings partially turned to plastic deformation to present a lower wear rate than that of the CrBN coating.展开更多
目的建立用于筛选具有抗肿瘤活性的沙利度胺衍生物的分子平台。方法从人胎肝c DNA文库中分别扩增cereblon(CRBN)和Ikaros家族锌指蛋白1(Ikaros family zinc finger protein 1,IKZF1)c DNA,并将其克隆入p XJ40-myc和pc DNA3-FLAG载体中,...目的建立用于筛选具有抗肿瘤活性的沙利度胺衍生物的分子平台。方法从人胎肝c DNA文库中分别扩增cereblon(CRBN)和Ikaros家族锌指蛋白1(Ikaros family zinc finger protein 1,IKZF1)c DNA,并将其克隆入p XJ40-myc和pc DNA3-FLAG载体中,将其转染293T细胞,通过检测沙利度胺及其衍生物对CRBN和IKZF1蛋白相互作用的改变,间接反映其在肿瘤抑制中的作用。结果 CRBN和IKZF1在293T细胞中均得到表达,沙利度胺及其两个衍生物可显著增强CRBN和IKZF1的结合。结论成功建立了用于筛选具有抗肿瘤活性的沙利度胺衍生物分子平台,初步发现2个沙利度胺衍生物可显著增强CRBN和IKZF1的结合,提示其具有潜在的抗癌活性。展开更多
文摘目的:探讨CRBN基因在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其与MAPK信号通路的关系。方法:通过GEO、GEPIA、TCGA等数据库分析来那度胺作用靶点CRBN在DLBCL中的功能及其与相关信号通路之间的关系,并收集2016年01月至2020年10月确诊为DLBCL,且经过来那度胺治疗的病例57例,免疫组化染色(IHC)和多重免疫荧光检测CRBN、MAPK的蛋白表达,进行统计分析。结果:通过生物信息学数据分析发现,CRBN通过MAPK信号通路影响细胞增殖和免疫微环境。CRBN和MAPK在DLBCL中高表达,且CRBN的表达与MAPK表达呈正相关(P<0.05);CRBN和MAPK高表达患者容易从来那度胺治疗中获益(P<0.05)。结论:CRBN可能通过MAPK信号通路对DLBCL的发生发展起作用,CRBN和MAPK蛋白的表达可以作为来那度胺疗效的评估参考依据。
基金This work has been financially supported by the National Natural Science Foundation of China(Grant No.51705245)Natural Science Foundation of Jiangsu Province(Grant No.BK20170794)National Key Laboratory of Science and Technology on Helicopter Transmission,Nanjing University of Aeronautics and Astronautics(Grant No.HTL-A-20G06).
文摘To compare the merits of Ni and Cu,the mechanical and tribological properties of CrBN coatings modified by Ni or Cu incorporation were studied.The results demonstrated that the CrBN–Cu coatings presented a lower friction coefficient than CrBN and CrBN–Ni coatings owing to the improved lubrication effect of the CuO layer originating from the tribochemical reaction.However,the hardness decline due to Cu incorporation was much greater than that of Ni incorporation.Thus,the CrBN–Cu coatings exhibited a higher wear rate than the CrBN coating.In contrast,the plastic deformation enhancement induced by Ni incorporation exceeded the hardness decline.Therefore,the wear of CrBN–Ni coatings partially turned to plastic deformation to present a lower wear rate than that of the CrBN coating.
