Searching and designing new materials play crucial roles in the development of energy storage devices. In today's world where machine learning technology has shown strong predictive ability for various tasks, the ...Searching and designing new materials play crucial roles in the development of energy storage devices. In today's world where machine learning technology has shown strong predictive ability for various tasks, the combination with machine learning technology will accelerate the process of material development. Herein, we develop ESM Cloud Toolkit for energy storage materials based on Mat Elab platform, which is designed as a convenient and accurate way to automatically record and save the raw data of scientific research. The ESM Cloud Toolkit includes multiple features such as automatic archiving of computational simulation data, post-processing of experimental data, and machine learning applications. It makes the entire research workflow more automated and reduces the entry barrier for the application of machine learning technology in the domain of energy storage materials. It integrates data archive, traceability, processing, and reutilization, and allows individual research data to play a greater role in the era of AI.展开更多
Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.I...Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.In this study,we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa,a variety widely cultivated in Northeast China.Subsequently,we created a single transcript CRISPR/Cas9(CRISPR_2.0)toolkit,incorporating multiplex gRNAs,designed for gene editing in Gongnong 1.Both Cas9 and gRNA scaffolds were under the control of the Arabidopsis ubiquitin-10 promoter,a widely employed polymeraseⅡconstitutive promoter known for strong transgene expression in dicots.To assess the toolkit’s efficiency,we targeted PALM1,a gene associated with a recognizable multifoliate phenotype.Utilizing the CRISPR_2.0 toolkit,we directed PALM1 editing at two sites in the wild-type Gongnong 1.Results indicated a 35.1%occurrence of editing events all in target 2 alleles,while no mutations were detected at target 1 in the transgenic-positive lines.To explore more efficient sgRNAs,we developed a rapid,reliable screening system based on Agrobacterium rhizogenes-mediated hairy root transformation,incorporating the visible reporter MtLAP1.This screening system demonstrated that most purple visible hairy roots underwent gene editing.Notably,sgRNA3,with an 83.0%editing efficiency,was selected using the visible hairy root system.As anticipated,tetra-allelic homozygous palm1 mutations exhibited a clear multifoliate phenotype.These palm1 lines demonstrated an average crude protein yield increase of 21.5%compared to trifoliolate alfalfa.Our findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.展开更多
为解决传统BIM软件的PPR管构件不完善、进行给排水设计时自动化和智能化程度低的问题,提出在Creo平台中设计PPR冷热水系统的新方案。详细研究了三维标准库、数据库存储与访问和自动布管模块的开发步骤和流程,在Creo2.0的平台上,利用Micr...为解决传统BIM软件的PPR管构件不完善、进行给排水设计时自动化和智能化程度低的问题,提出在Creo平台中设计PPR冷热水系统的新方案。详细研究了三维标准库、数据库存储与访问和自动布管模块的开发步骤和流程,在Creo2.0的平台上,利用Microsoft Visual Studio 2010和二次开发工具Pro/Toolkit开发出了具有可视化界面的PPR三维标准库和自动布管系统,该系统可以快速生成符合国家标准的PPR管材、管件并实现了冷热水系统的自动装配布管功能,通过实例验证了该系统的准确性和高效性。展开更多
论述了基于Creo三维平台的零件快速变型设计系统的二次开发,并针对此系统开发中的关键技术和系统功能进行了详细的介绍。在Visual Studio 2010环境下,利用Creo软件本身提供的二次开发工具Creo/TOOLKIT,结合MFC技术设计编写了具有通用性...论述了基于Creo三维平台的零件快速变型设计系统的二次开发,并针对此系统开发中的关键技术和系统功能进行了详细的介绍。在Visual Studio 2010环境下,利用Creo软件本身提供的二次开发工具Creo/TOOLKIT,结合MFC技术设计编写了具有通用性的零件快速变型设计系统。以带颈法兰的变型设计为例,介绍了系统的具体功能。工程应用表明,该系统能够对零件进行快速变型设计,同时也为设计人员快速设计新的零件产品及实现产品的系列化提供了新方法,减小了设计人员的工作量,提高了设计效率。展开更多
提高设计效率的角度出发,对如何在Creo平台下实现零件的参数化变型设计进行了探究,并以一种新的方法实现了零件的参数化变型设计和零件的系列化。结合Visual Studio 2010集成开发环境和Creo软件二次开发工具Creo/TOOLKIT,利用MFC技术实...提高设计效率的角度出发,对如何在Creo平台下实现零件的参数化变型设计进行了探究,并以一种新的方法实现了零件的参数化变型设计和零件的系列化。结合Visual Studio 2010集成开发环境和Creo软件二次开发工具Creo/TOOLKIT,利用MFC技术实现了零件的参数化变型设计。以阶梯轴的参数化变型设计为例,对该方法进行了验证,结果表明该方法避免了设计者的重复劳动,提高了零件的变型设计效率,更好的实现零件产品的系列化。展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 52022106 and 52172258)the Informatization Plan of Chinese Academy of Sciences (Grant No. CASWX2021SF-0102)。
文摘Searching and designing new materials play crucial roles in the development of energy storage devices. In today's world where machine learning technology has shown strong predictive ability for various tasks, the combination with machine learning technology will accelerate the process of material development. Herein, we develop ESM Cloud Toolkit for energy storage materials based on Mat Elab platform, which is designed as a convenient and accurate way to automatically record and save the raw data of scientific research. The ESM Cloud Toolkit includes multiple features such as automatic archiving of computational simulation data, post-processing of experimental data, and machine learning applications. It makes the entire research workflow more automated and reduces the entry barrier for the application of machine learning technology in the domain of energy storage materials. It integrates data archive, traceability, processing, and reutilization, and allows individual research data to play a greater role in the era of AI.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA26030301)Hohhot Key R&D Project(2023-JBGSS-1),the National Natural Science Foundation of China(U23A200206,32071864,32325035)+1 种基金the Taishan Scholar Program of Shandong(to Chunxiang Fu)the Shandong Provincial Natural Science Foundation(ZR202210270038)。
文摘Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.In this study,we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa,a variety widely cultivated in Northeast China.Subsequently,we created a single transcript CRISPR/Cas9(CRISPR_2.0)toolkit,incorporating multiplex gRNAs,designed for gene editing in Gongnong 1.Both Cas9 and gRNA scaffolds were under the control of the Arabidopsis ubiquitin-10 promoter,a widely employed polymeraseⅡconstitutive promoter known for strong transgene expression in dicots.To assess the toolkit’s efficiency,we targeted PALM1,a gene associated with a recognizable multifoliate phenotype.Utilizing the CRISPR_2.0 toolkit,we directed PALM1 editing at two sites in the wild-type Gongnong 1.Results indicated a 35.1%occurrence of editing events all in target 2 alleles,while no mutations were detected at target 1 in the transgenic-positive lines.To explore more efficient sgRNAs,we developed a rapid,reliable screening system based on Agrobacterium rhizogenes-mediated hairy root transformation,incorporating the visible reporter MtLAP1.This screening system demonstrated that most purple visible hairy roots underwent gene editing.Notably,sgRNA3,with an 83.0%editing efficiency,was selected using the visible hairy root system.As anticipated,tetra-allelic homozygous palm1 mutations exhibited a clear multifoliate phenotype.These palm1 lines demonstrated an average crude protein yield increase of 21.5%compared to trifoliolate alfalfa.Our findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.
文摘为解决传统BIM软件的PPR管构件不完善、进行给排水设计时自动化和智能化程度低的问题,提出在Creo平台中设计PPR冷热水系统的新方案。详细研究了三维标准库、数据库存储与访问和自动布管模块的开发步骤和流程,在Creo2.0的平台上,利用Microsoft Visual Studio 2010和二次开发工具Pro/Toolkit开发出了具有可视化界面的PPR三维标准库和自动布管系统,该系统可以快速生成符合国家标准的PPR管材、管件并实现了冷热水系统的自动装配布管功能,通过实例验证了该系统的准确性和高效性。
文摘论述了基于Creo三维平台的零件快速变型设计系统的二次开发,并针对此系统开发中的关键技术和系统功能进行了详细的介绍。在Visual Studio 2010环境下,利用Creo软件本身提供的二次开发工具Creo/TOOLKIT,结合MFC技术设计编写了具有通用性的零件快速变型设计系统。以带颈法兰的变型设计为例,介绍了系统的具体功能。工程应用表明,该系统能够对零件进行快速变型设计,同时也为设计人员快速设计新的零件产品及实现产品的系列化提供了新方法,减小了设计人员的工作量,提高了设计效率。
文摘提高设计效率的角度出发,对如何在Creo平台下实现零件的参数化变型设计进行了探究,并以一种新的方法实现了零件的参数化变型设计和零件的系列化。结合Visual Studio 2010集成开发环境和Creo软件二次开发工具Creo/TOOLKIT,利用MFC技术实现了零件的参数化变型设计。以阶梯轴的参数化变型设计为例,对该方法进行了验证,结果表明该方法避免了设计者的重复劳动,提高了零件的变型设计效率,更好的实现零件产品的系列化。
