Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon S...Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs （Operational Taxonomic Units） in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems （including desert lakes） worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs （including OTU3615, OTU6535, and OTU6646） between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems （including desert lakes） worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.展开更多
Goats are globally very important domestic small ruminants being kept for meat, milk, skin, byproducts and several socio-cultural purposes. Goat farmers in the Lake Albert Crescent Zone (LACZ) of Uganda identified dry...Goats are globally very important domestic small ruminants being kept for meat, milk, skin, byproducts and several socio-cultural purposes. Goat farmers in the Lake Albert Crescent Zone (LACZ) of Uganda identified dry-season feed scarcity, poor breeds, diseases, informal marketing and theft as major constraints which underscore the scarcity of information on improved management technologies and the need for targeted decisions. At BuZARDI goat farm, a semi-intensive farm that is also experiencing similar challenges, we documented management practices and collected production related data for 2 years. We computed flock dynamics and mortality rates purposely to influence decisions aimed at increasing the farm’s productivity. Data were collected using pens and books, validated and analyzed using MS office 2013 and SPSS version 22. The flock size increased from 37 goats in July 2020 to 175 goats in June 2022. One hundred twenty-five goats were introduced into the farm as breeding stock, 89 kids were born on the farm and 61 goats died due to various causes. The average number of goats in the farm per month was 109.2 (SEM = 13.7), female 79.8 (SEM = 10.8) and male 29.4, (SEM = 9.9). Average population of goats at risk of death was 95 and the Mortality rate was 27 goats per 1000 goat months. The disaggregated mortality rate of 5 goats per 1000 goat months and 39 goats per 1000 goat months were obtained for the period FY 2020-2021 and FY2021-2022 respectively. The main contributors to the mortality of goats were worm infestation (33%), fracture and injuries arising from stampedes (18%), Ticks and tick borne diseases (18%), respiratory tract infections (11%) and malnutrition (10%). We recommend strict adherence to the flock health program;improved parasite management, separation & improved care for kids, vaccinations, safer feeding, and evidence based disease management, adoption of digital data collection tools and development of an appropriate app for more accurate determination of mortality rates.展开更多
基金supported by the National Natural Science Foundation of China(41271265,31570498,31300411)the Science and Technology Projects in Gansu Province(1304NKCA135)the Foundation of China Scholarship Council
文摘Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs （Operational Taxonomic Units） in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems （including desert lakes） worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs （including OTU3615, OTU6535, and OTU6646） between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems （including desert lakes） worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.
文摘Goats are globally very important domestic small ruminants being kept for meat, milk, skin, byproducts and several socio-cultural purposes. Goat farmers in the Lake Albert Crescent Zone (LACZ) of Uganda identified dry-season feed scarcity, poor breeds, diseases, informal marketing and theft as major constraints which underscore the scarcity of information on improved management technologies and the need for targeted decisions. At BuZARDI goat farm, a semi-intensive farm that is also experiencing similar challenges, we documented management practices and collected production related data for 2 years. We computed flock dynamics and mortality rates purposely to influence decisions aimed at increasing the farm’s productivity. Data were collected using pens and books, validated and analyzed using MS office 2013 and SPSS version 22. The flock size increased from 37 goats in July 2020 to 175 goats in June 2022. One hundred twenty-five goats were introduced into the farm as breeding stock, 89 kids were born on the farm and 61 goats died due to various causes. The average number of goats in the farm per month was 109.2 (SEM = 13.7), female 79.8 (SEM = 10.8) and male 29.4, (SEM = 9.9). Average population of goats at risk of death was 95 and the Mortality rate was 27 goats per 1000 goat months. The disaggregated mortality rate of 5 goats per 1000 goat months and 39 goats per 1000 goat months were obtained for the period FY 2020-2021 and FY2021-2022 respectively. The main contributors to the mortality of goats were worm infestation (33%), fracture and injuries arising from stampedes (18%), Ticks and tick borne diseases (18%), respiratory tract infections (11%) and malnutrition (10%). We recommend strict adherence to the flock health program;improved parasite management, separation & improved care for kids, vaccinations, safer feeding, and evidence based disease management, adoption of digital data collection tools and development of an appropriate app for more accurate determination of mortality rates.
