Preliminary phytochemical analysis,antibacterial,antifungal and anticandidal activities of successive extracts of Crossandra infundibuliformis

Objective:To investigate the phytochemical,antibacterial,antifungal and anticandidal activity of successive extracts of Crossandra infundibuliformis(Acanthaceae) leaves.Methods:Preliminary screening on the presence of alkaloids,saponins,phytosterols,phenolic compounds,flavanoids, tannins,carbohydrates,terpenoids,oils and fats were carried out by phytochemical analysis.The antibacterial,antifungal and anticandidal activities were done by agar well diffusion technique. Results:The successive extracts have an array of chemical constituents and the MIC values of antibacterial activity ranges from 0.007 8 to 0.015 0μg/mL.In case of antifungal and anticandidal activities the MIC values were between 0.125 and 0.250μg/mL.Conclusions:These findings demonstrate that the leaf extracts of C.infimdibuliformis presents excellent antimicrobial activities and thus have great potential as a source for natural health care products.

Hepatoprotective potential of petroleum ether leaf extract of Crossandra infundibuliformis on CCl_4 induced liver toxicity in albino mice

Objective:To analyze the hepatoprotective effect of the Crossandra infundibuliformis.Methods: Hepatotoxicity was induced by carbon tetrachloride.Petroleum ether extract of dried leaves was administrated to mice for 7 days.The hepatoprotective effect of petroleum ether extract was evaluated by the assay of liver function biochemical parameters.Results:The result clearly indicates that petroleum ether extract showed significant hepatoprotection when compared with standard Silumarin.Conclusions:The petroleum ether extract of the leaves of Crossandra infundibuliformis possess significant acute hepatoprotective activity.Thus further investigation on this species would bring a promising drug for liver disorders.

Evaluation of Phenolic Content and Antioxidant Property of <i>Crossandra infundibuliformis</i>Leaves Extracts

The present study was aimed to investigate the phenolic content and antioxidant property of Crossandra infundibuliformis leaves. Shade dried leaves were successively extracted and determined phenolic and flavonol content of the extracts. Six different in vitro antioxidant assays such as 1, 1’-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide, hydrogen peroxide radical scavenging, reducing power of the extracts and total antioxidant capacity (values were expressed as mg ascorbic acid (vitamin C) equivalent antioxidant capacity per gram of extracts) assays were followed to determine the antioxidant capacity of the extracts. The results of this study indicated that methanol extract possess higher level of phenolic (98.52 mg of gallic acid/g of extract) and flavonol (84.59 mg of rutin/g of extract) constituents. The antioxidant property of ethyl acetate extract was significantly higher than other extracts in DPPH method. Moreover, methanol extract has significantly higher antioxidant activity in LPO inhibition (IC50 value of 70.66 μg/ml), hydrogen peroxide (IC50 value 130.33 μg/ml) and reducing capacity of the extract (1.19) than other chloroform and ethyl acetate extracts. The strong correlation was observed for phenolic content and antioxidant activities of the extracts ensuring the involvement of phenols in antioxidant activity. However, results suggests that Crossandra infundibuliformis leaves possess antioxidant activity.

多因子正交试验对鞭炮花丛生芽诱导条件的筛选

利用正交试验设计方法探讨6-BA、NAA、2,4-D和蔗糖对丛生芽诱导影响。结果表明:蔗糖和6-BA的浓度对鞭炮花丛生芽诱导的影响最大,高浓度的2,4-D对于愈伤组织的诱导和增殖具有良好的促进作用,但对芽的分化有抑制作用。筛选出诱导鞭炮花丛生芽的最适培养基为MS+2.0 mg/L 6-BA+1.4 mg/L NAA+25 g/L蔗糖+0.7%琼脂。该培养基可使外植体一步诱导出苗,并获得移栽成活的植株。

不同处理方法对鸟尾花种子萌发的影响

在西双版纳地区进行鸟尾花引种繁殖试验,研究了不同的培养温度、浸种温度和不同浓度的3种药剂(KH_(2)PO_(4)、GA_(3)和6-BA)浸种处理对种子的发芽率、发芽势和发芽指数的影响。结果表明:45℃清水浸种可促进鸟尾花种子的萌发,在培养箱中变温(25℃/35℃)条件下,发芽情况最好,在同等条件下用200 mg·L^(-1) GA_(3)浸种或100 mg·L^(-1)6-BA浸种处理,鸟尾花种子萌发效果最好。