Background：Algae are widely recognized for their high oil content and for exponentially accumulating biomass with particular potential to provide single cell protein for human consumption or animal feed.It is believe...Background：Algae are widely recognized for their high oil content and for exponentially accumulating biomass with particular potential to provide single cell protein for human consumption or animal feed.It is believed that along with biodiesel from algae,the high protein de-oiled algal residue may become an alternative feed supplement option in the future.This study was conducted to investigate de-oiled algal residue obtained from the common Chlorella species,Thalassiosira weissflogii,Selenarstrum capricornutum,Scenedesmus sp.,and Scenedesmus dimorphus for assessment as potential feed supplements for ruminants by comparing with soybean（Glycine max） meal and alfalfa（Medicago sativa） hay.Results：With the exception of T.weissflogii,algal residue had higher concentrations of Cu,Zn,and Mn and lower concentration of Ca,Mg,and K than soybean meal and alfalfa hay.The algal residue CP（crude protein）concentrations ranged from 140 to 445 g/kg DM and varied among the de-oiled residues.In vitro rumen fermentation gas accumulation curves indicated that algal biomass degradation potential was less than that of soybean meal or alfalfa hay by up to 41.7%.The gas production curve,interpreted with a dual pool logistic model,confirmed that the fraction sizes for fast fermenting and slow fermenting of de-oiled algal residues were smaller than those in soybean meal and alfalfa hay,and the fermenting rate of the fractions was also low.Conclusions：Inferior in vitro rumen gas accumulation from the five de-oiled algal residues suggests that these algal byproducts are less degradable in the rumen.展开更多
The Sinian-Cambrian formations of the Sichuan Basin have favorable hydrocarbon accumulation conditions,but the exploration for large-scale gas fields is quite challenging due to old strata and multiple tectonic moveme...The Sinian-Cambrian formations of the Sichuan Basin have favorable hydrocarbon accumulation conditions,but the exploration for large-scale gas fields is quite challenging due to old strata and multiple tectonic movements.Since the Weiyuan Sinian large gas field was found in 1964,the largest monoblock gas field(Anyue Gasfield)was discovered in the Cambrian Longwangmiao Formation of the Moxi region in 2013 with proven gas reserves of 440.1×109 m3.Total proven,probable and possible reserves exceed one trillion cubic meters in the Sinian Dengying Formation and the Cambrian Longwangmiao Formation of the Gaoshiti-Moxi region.The natural gas components,light hydrocarbons,reservoir bitumen abundance and other evidences prove that the dry natural gas was mainly derived from oil-cracking,with methane(a content of 82.65%-97.35%),ethane(a content of 0.01%-0.29%),nitrogen(a content of 0.44%-6.13%),helium(a content of 0.01%-0.06%),and hydrogen sulphide(0.62-61.11 g/m^(3)).Gas reservoir pressure increases gradually from the Sinian normal pressure(a pressure coefficient of 1.07-1.13)to high pressure(a pressure coefficient of 1.53-1.70)in the Cambrian Longwangmiao Formation.The temperature of the gas reservoir is 137.5-163 ℃.Gas reservoir traps are divided into three categories:tectonic type,tectonic-formation type and tectonic-lithologic type.The large-scale enrichment of the Sinian-Cambrian natural gas results from effective configuration of the large stable inherited palaeo-uplift during the Tongwan tectonic movement,wide distribution of ancient source rocks,high-quality reservoirs with vast pore-cavity,crude oil cracking of large palaeo-reservoirs and favorable preservation conditions.According to the palaeo-structure pattern prior to crude oil cracking of the palaeo-reservoirs,and bitumen abundance as well as the distribution characteristics of current gas reservoirs,the accumulation patterns of the cracking gas reservoir can be classified into three types:accumulation type,semi-accumulation and semi-dispersion type,and dispersion type.This understanding will play an important role in guiding the exploration of the Sinian-Cambrian natural gas exploration in the Sichuan Basin.展开更多
基金supported by Louisiana Board of Regents Research grant
文摘Background：Algae are widely recognized for their high oil content and for exponentially accumulating biomass with particular potential to provide single cell protein for human consumption or animal feed.It is believed that along with biodiesel from algae,the high protein de-oiled algal residue may become an alternative feed supplement option in the future.This study was conducted to investigate de-oiled algal residue obtained from the common Chlorella species,Thalassiosira weissflogii,Selenarstrum capricornutum,Scenedesmus sp.,and Scenedesmus dimorphus for assessment as potential feed supplements for ruminants by comparing with soybean（Glycine max） meal and alfalfa（Medicago sativa） hay.Results：With the exception of T.weissflogii,algal residue had higher concentrations of Cu,Zn,and Mn and lower concentration of Ca,Mg,and K than soybean meal and alfalfa hay.The algal residue CP（crude protein）concentrations ranged from 140 to 445 g/kg DM and varied among the de-oiled residues.In vitro rumen fermentation gas accumulation curves indicated that algal biomass degradation potential was less than that of soybean meal or alfalfa hay by up to 41.7%.The gas production curve,interpreted with a dual pool logistic model,confirmed that the fraction sizes for fast fermenting and slow fermenting of de-oiled algal residues were smaller than those in soybean meal and alfalfa hay,and the fermenting rate of the fractions was also low.Conclusions：Inferior in vitro rumen gas accumulation from the five de-oiled algal residues suggests that these algal byproducts are less degradable in the rumen.
基金This work was funded by National Science and Technology Major Project of China(Grant No.2011ZX05007)PetroChina Exploration and Production Special Project“Evaluation and associated exploration technology research of Sinian hydrocarbon-bearing reservoir in Leshan-Longnvsi Paleo-uplift of the Sichuan Basin”.
文摘The Sinian-Cambrian formations of the Sichuan Basin have favorable hydrocarbon accumulation conditions,but the exploration for large-scale gas fields is quite challenging due to old strata and multiple tectonic movements.Since the Weiyuan Sinian large gas field was found in 1964,the largest monoblock gas field(Anyue Gasfield)was discovered in the Cambrian Longwangmiao Formation of the Moxi region in 2013 with proven gas reserves of 440.1×109 m3.Total proven,probable and possible reserves exceed one trillion cubic meters in the Sinian Dengying Formation and the Cambrian Longwangmiao Formation of the Gaoshiti-Moxi region.The natural gas components,light hydrocarbons,reservoir bitumen abundance and other evidences prove that the dry natural gas was mainly derived from oil-cracking,with methane(a content of 82.65%-97.35%),ethane(a content of 0.01%-0.29%),nitrogen(a content of 0.44%-6.13%),helium(a content of 0.01%-0.06%),and hydrogen sulphide(0.62-61.11 g/m^(3)).Gas reservoir pressure increases gradually from the Sinian normal pressure(a pressure coefficient of 1.07-1.13)to high pressure(a pressure coefficient of 1.53-1.70)in the Cambrian Longwangmiao Formation.The temperature of the gas reservoir is 137.5-163 ℃.Gas reservoir traps are divided into three categories:tectonic type,tectonic-formation type and tectonic-lithologic type.The large-scale enrichment of the Sinian-Cambrian natural gas results from effective configuration of the large stable inherited palaeo-uplift during the Tongwan tectonic movement,wide distribution of ancient source rocks,high-quality reservoirs with vast pore-cavity,crude oil cracking of large palaeo-reservoirs and favorable preservation conditions.According to the palaeo-structure pattern prior to crude oil cracking of the palaeo-reservoirs,and bitumen abundance as well as the distribution characteristics of current gas reservoirs,the accumulation patterns of the cracking gas reservoir can be classified into three types:accumulation type,semi-accumulation and semi-dispersion type,and dispersion type.This understanding will play an important role in guiding the exploration of the Sinian-Cambrian natural gas exploration in the Sichuan Basin.
