The impact of chestnut blight, caused by the fungal pathogen Cryphonectria parasitica, has diminished in Europe due to a natural biological control caused by hypovirus infection. Hypovirulence-mediated biological cont...The impact of chestnut blight, caused by the fungal pathogen Cryphonectria parasitica, has diminished in Europe due to a natural biological control caused by hypovirus infection. Hypovirulence-mediated biological control has been far less successful in North America meriting further evaluation of field isolates that have the ability to produce non-lethal cankers, generate hypovirulent inoculum, and exhibit a greater ecological fitness in forest systems. In this study, Cryphonectria hypoviruses (CHV) CHV3-County Line, CHV1-Euro7, and CHV1-Ep713 were evaluated in five different isolates of C. parasitica. One hundred and eighty cankers representing each treatment combination were initiated on American chestnut sprouts in the Monongahela National Forest, West Virginia, USA. The size of cankers, the persistence of hypovirulent (HV) isolates, stroma production, and hypovirus transmission to conidia were assessed four and 12 months after canker expansion. CHV3-County Line infected isolates produced significantly smaller cankers than the isolates infected with either CHV1-type. With regard to CHV1-Euro7 isolates, the fungal genome appeared to contribute to the differences in canker size. After four months, HV isolates harboring either CHV1-type (30%) were retrieved at a significantly higher rate than isolates containing CHV3-County Line (14%). After 12 months, the HV recovery was similar among the three hypoviruses indicating smaller cankers will maintain their HV status after one year. Very few stroma were produced after one year in the field from HV isolates. In vitro, CHV3-County Line (49%) had a significantly lower rate of hypovirus transmission to conidia when compared to CHV1-Euro7 (87%) and CHV1-Ep713 (80%). Significant differences existed among the five different isolates indicating HV transmission is dependent on the fungal genome. This research provided additional evidence that each hypovirus interacts with its host differently and certain isolate/hypovirus combinations have better biological control potential than others.展开更多
The effects of virus strains on the virus transmission in Cryphonectria parasitica were observed in this study. In laboratory assays by replicating vegetative incompatibility (vic) genotypes in independent fungal isol...The effects of virus strains on the virus transmission in Cryphonectria parasitica were observed in this study. In laboratory assays by replicating vegetative incompatibility (vic) genotypes in independent fungal isolates, we quantified the transmission of three CHV1 viruses between isolates heteroallelic at one or two vic loci, and demonstrated that different CHV1 virus isolates determined their transmission abilities. The results suggested that using the hypoviruses with higher transmission abilities could increase the efficiencies of the biological control of chestnut blight.展开更多
Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treei...Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treeincluding various cultivars such as Tsukuba, Okkwang, Rihei, Ginyose, Eunsan, etc. were tested with a rapid, simple and reliable method. The cultivar Banseki is one of the most resistant cultivars tested and has the necrotic area of 0.40 cm2. However, the necrotic areas of the cultivars of American chestnut, Eunsan, Kwangjujoyul and Eaton, ranges from 3.35 to 2.61 cm2 and these cultivars are relatively susceptible to this pathogen. Many pycnidia of this pathogen were observed on the inner and outer bark tissues after incubating for 3 weeks.展开更多
文摘The impact of chestnut blight, caused by the fungal pathogen Cryphonectria parasitica, has diminished in Europe due to a natural biological control caused by hypovirus infection. Hypovirulence-mediated biological control has been far less successful in North America meriting further evaluation of field isolates that have the ability to produce non-lethal cankers, generate hypovirulent inoculum, and exhibit a greater ecological fitness in forest systems. In this study, Cryphonectria hypoviruses (CHV) CHV3-County Line, CHV1-Euro7, and CHV1-Ep713 were evaluated in five different isolates of C. parasitica. One hundred and eighty cankers representing each treatment combination were initiated on American chestnut sprouts in the Monongahela National Forest, West Virginia, USA. The size of cankers, the persistence of hypovirulent (HV) isolates, stroma production, and hypovirus transmission to conidia were assessed four and 12 months after canker expansion. CHV3-County Line infected isolates produced significantly smaller cankers than the isolates infected with either CHV1-type. With regard to CHV1-Euro7 isolates, the fungal genome appeared to contribute to the differences in canker size. After four months, HV isolates harboring either CHV1-type (30%) were retrieved at a significantly higher rate than isolates containing CHV3-County Line (14%). After 12 months, the HV recovery was similar among the three hypoviruses indicating smaller cankers will maintain their HV status after one year. Very few stroma were produced after one year in the field from HV isolates. In vitro, CHV3-County Line (49%) had a significantly lower rate of hypovirus transmission to conidia when compared to CHV1-Euro7 (87%) and CHV1-Ep713 (80%). Significant differences existed among the five different isolates indicating HV transmission is dependent on the fungal genome. This research provided additional evidence that each hypovirus interacts with its host differently and certain isolate/hypovirus combinations have better biological control potential than others.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 30370918, 30470062)
文摘The effects of virus strains on the virus transmission in Cryphonectria parasitica were observed in this study. In laboratory assays by replicating vegetative incompatibility (vic) genotypes in independent fungal isolates, we quantified the transmission of three CHV1 viruses between isolates heteroallelic at one or two vic loci, and demonstrated that different CHV1 virus isolates determined their transmission abilities. The results suggested that using the hypoviruses with higher transmission abilities could increase the efficiencies of the biological control of chestnut blight.
文摘Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treeincluding various cultivars such as Tsukuba, Okkwang, Rihei, Ginyose, Eunsan, etc. were tested with a rapid, simple and reliable method. The cultivar Banseki is one of the most resistant cultivars tested and has the necrotic area of 0.40 cm2. However, the necrotic areas of the cultivars of American chestnut, Eunsan, Kwangjujoyul and Eaton, ranges from 3.35 to 2.61 cm2 and these cultivars are relatively susceptible to this pathogen. Many pycnidia of this pathogen were observed on the inner and outer bark tissues after incubating for 3 weeks.
