Inhibitory Effect of Flavonoid Glycosides from Chlorophytum comosum on Nasopharyngeal Carcinoma 5-8F Cells and Its Mechanism

[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.

BR_(0^-)代数的表示定理及其简化形式

通过对基础模糊命题演算系统BL*及相应的Lindenbaum代数的研究,给出了BR_(0^-)代数的格蕴涵表示形式,使得BR_(0^-)代数从定义形式上更加符合逻辑代数的特征,突出了BR_(0^-)代数和其他逻辑代数的区别与联系,其次,结合MV-代数,R_(0^-)代数和BR_(0^-)代数的关系给出了MV-代数的BR_(0^-)代数表示形式以及BR_(0^-)代数的简化形式.

关于BR_0-代数的一些新性质

基于R0-代数(BR0-代数)对于模糊命题逻辑系统L*(BL*)的语义的重要性,对R0-代数和BR0-代数作更进一步的探讨,得到了它们的一些新的性质以及BR0-代数成为R0-代数的充分必要条件.这些结果将有助于对相应的形式逻辑系统与模糊推理的研究.

Optimization of Microwave-assisted Extraction Technology of Polyphenols from Loropetalum chinense(R. Br.) Oliv

In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.

BR_0代数与FI代数的关系

研究了模糊命题演算形式演绎系统L*和与之在语义上相匹配的R0代数,以及基础R0代数和基础L*系统,提出了BR0代数的简化形式的概念,讨论了BR0代数与FI代数的相互关系。

Determination of contents of eight alkaloids in fruits of Macleaya cordata (Willd) R. Br. from different habitats and antioxidant activities of extracts

A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients （R^2〉0.999 6） and recoveries （from 97.3% to 104.9%） were acceptable. 1,1-diphenyl-2-picrylhydrazyl （DPPH） method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant aetivity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids.

BR_0-代数的简化及FI-代数的关系

研究了王国俊教授建立的模糊命题演算的形式演绎系统L*和与之在语义上相匹配的R0-代数以及吴洪博教授提出的基础R0-代数和基础L*系统,提出了WBR0-代数的观点,讨论了它与BR0-代数的关系,简化了BR0-代数的定义,在讨论正则FI-代数与正则剩余格之间关系的基础上,讨论了BR0-代数与FI-代数的相互关系.

Stability of Sanguinarine and Chelerythrine in Macleaya cordata (Willd.) R. Br.

[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.

非球谐振子势V(r)=Ar^2+Br^4+Cr^6 schrodinger方程的解析解

根据波函数的有限性和非球谐振子势的渐近性质,通过待定非球谐振子势波函数的设定,得到势函数表示为V(r)=Ar2+Br4+Cr6的schrodinger方程的精确的能量本征值和本征波函数.通过对势参数制约关系、能量本征值和本征波函数的分析,得到相同势参数制约关系下多组能量本征值和本征波函数.

Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits

Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.

Optimization of Extraction Technology of Pigments from Petals of Matthiola incana(L.)R.Br.of Different Colors

[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.

A NEW GUANIDINE DERIVATIVE FROM ASTRAGALUS COMPLANATUS R.Br.

A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallographic analysis to be N-[3-carboxypropyl]-N-[3-methyl-2-butenyl]guanidine.

RP-HPLC法同时测定藏药手掌参中3种抗AD活性成分的含量

[目的]建立同时测定藏药手掌参中天麻素、dactylorhin A和militarine的含量测定方法.[方法]采用反相高效液相色谱法（RP-HPLC）,以ZORBAX SB-C18（4.6 mm×250 mm,5 μm）色谱柱;进样10μl;乙腈-浓度0.5％的磷酸水梯度洗脱（0 min,7％;10 min,15％;20 min,20％;25 min,23％;30 min,40％;35 min,40％）;流速为0.7 ～ 1.0 ml/min;柱温30℃;检测波长222 nm.[结果]在35 min内完成对3种抗AD活性成分的分析,各成分之间均达到基线分离.天麻素、dactylorhin A和militarine的线性范围分别为：0.031 34～1.003 00mg/ml（R=0.999 8）、0.038 06 ～1.218 00 mg/ml（R =0.999 6）、0.039 28 ～1.257 00 mg/ml（R=0.999 6）;平均加样回收率（n=6）分别为98.88％、99.91％和100.48％.测定得青藏高原15个不同地区不同种属的手参中3种抗AD活性成分的含量,以互助变异手参的含量最高.[结论]该方法建立的HPLC分析方法简单准确,能快速测定手参中有效成分的含量,青藏高原不同地区不同种属手参中3种抗AD活性成分含量差异较大.

苯达莫司汀联合利妥昔单抗与R-CHOP方案一线治疗惰性非霍奇金淋巴瘤或套细胞淋巴瘤疗效及安全性比较

利妥昔单抗联合包含环磷酰胺、阿霉素、长春新碱和泼尼松的R—CHOP方案是目前治疗进展期惰性非霍奇金淋巴瘤（indolentnon．Hodgkin’slymphoma，iNHL）和套细胞淋巴瘤（mantlecelllymphoma，MCL）常用的一线方案。

南方菟丝子18S rRNA基因片段的克隆与序列分析

根据拟南芥光敏色素B基因序列设计引物,RT-PCR扩增南方菟丝子同一基因相应片段,扩增使用了TD PCR技术,同时获得3个特异基因片段,对长约300 bp的片段克隆后进行序列分析,显示该片段与沼泽菟丝子和拟南芥18SrRNA基因相应片段的一致性分别为98.9%和97%,结果表明,该片段为南方菟丝子18S rRNA基因片段.

《Flora of China》中蒲葵属之分类订正(英文)

通过调查,对Livistona chinensis的后选模式、L.fengkaiensis和L.jenkinsiana的主模式、L.saribus的等新模式、L.speciosa的等模式与《Flora of China》中的上述种类作比较,证实《Flora of China》中的L.jenkinsiana是L.spe-ciosaKurz,后者被中国分类学文献(如《海南植物志》、《中国植物志》、《福建植物志》、《云南植物志》)误定为L.saribus。L.speciosa的果倒卵形、椭圆形或卵形,而L.jenkinsiana的果肾形或近球形。源于《中国植物志》的L.saribus并非原产于中国。在中国,L.speciosa原产于云南、广东、海南和福建,L.chinensis原产于广东和台湾,而L.jenkinsi-ana和L.saribus仅被引种至中国的植物园。

豆瓣菜种质资源遗传多样性的RAPD和ISSR分析

以8个豆瓣菜的品种为试材，用筛选出的79个RAPD引物和34个ISSR引物对这8个品种的基因组DNA进行扩增，分别扩增出361条和179条谱带，每个引物扩增出的带在3～10条之间，平均每个引物扩增出约5条带。根据所得的条带进行聚类分析，两种标记产生的聚类图存在一些差异，但它们都可以较好地将8个品种按亲缘关系的远近划分为3个不同的类群。Mantel测试得出相关系数r=0．58155，表明RAPD和ISSR两种分子标记技术的相关度很低。

Impact of Simulated Drought Stress on Soil Microbiology, and Nematofauna in a Native Shrub + Millet Intercropping System in Senegal

Drought stress strongly affects soil biota and impairs crop production, which under climate change will be exacerbated in semi-arid cropping regions such as the Sahel. Hence soil management systems are needed that can buffer against drought. In West Africa, field studies have found intercropping of millet with the native shrub Piliostigma reticulatum improves soil-plant-water relations, microbial activity and diversity, and suppress parasitic nematodes, which can significantly increase crop yield. However, little information is available on its beneficial or negative effects on soils or crops during water stress. Therefore, the objective was to investigate the impact of P. reticulatum in moderating water stress effects on soil properties and pearl millet (Pennisetum glaucum [L.] R. Br.) productivity. In the greenhouse, soil chemical and microbial properties and millet growth were investigated with a factorial experiment of varying levels of soil moisture (favorable, moderately stressed, or severely stressed water conditions) that was imposed for 55 days on soils containing sole P. reticulatum or millet, or millet + P. reticulatum. The results showed that the presence of P. reticulatum did not buffer soils against water stress in relation to soil chemical and microbial properties measured at the end of the experiment. Severe water stress did significantly decrease the height, number of leaves, and aboveground biomass of millet plants. Additionally, respiration, nematofauna trophic structure and abundance decreased as water stress increased. Lastly, bacterial feeders and plant parasitic nematodes were the most sensitive to severe water stress while fungal feeding nematodes remained unaffected. The results suggested that the intensity of water stress had more negative effects on soil basal respiration rather than soil microbial biomass.

手参属植物化学成分及药理活性研究进展

兰科手参属植物手参、西南手参、短距手参与峨眉手参等为珍稀药用植物,具有较高的临床药用价值,近年来逐渐受到国内外学者的关注。综述手参属植物化学成分和药理活性的研究进展,其中化学成分包括二氢类、菲类、苷类、苯丙素类、异戊二烯类、脂肪酸衍生物等多种类型;药理活性包括抗氧化、抗过敏、促进祖细胞增殖、抑制乙型肝炎病毒表面抗原等作用,以期为今后对该属植物进一步研究和开发利用提供参考。

燃油各组分在咪唑盐类离子液体中溶解性的研究

利用微波法合成了不同烷基取代的溴化1-烷基-3-甲基咪唑盐类离子液体[RMIm]Br(R=E,B,H;E表示乙基,B表示1-丁基,H表示1-庚基),并对其进行了红外光谱表征,用平衡法测定了燃油各基本组分的溶解性.结果表明:饱和烷烃在离子液体中几乎不溶,苯系物在离子液体中有一定的溶解度,而含S和含N化合物在离子液体中有较大的溶解度;升高温度有助于燃油各组分在离子液体中的溶解;离子液体的含水量对含S和含N化合物的溶解度影响较大.