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Significance of Cu/Zn-Superoxide Dismutase Levels in Hemodialysis Patients: A Mini Review
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作者 Yuya Nakamura Masahiro Inagaki +8 位作者 Sachiyo Kenmotsu Shiho Yamadera Isao Ohsawa Hiromichi Gotoh Yoshikazu Goto Naoki Sato Tatsunori Oguchi Mayumi Tsuji Yuji Kiuchi 《Modern Research in Inflammation》 2017年第2期9-13,共5页
Cu/Zn-superoxide dismutase (Cu/Zn-SOD) is an enzyme that is ubiquitously present in the cytoplasm and causes dismutation of superoxide radicals, therefore Cu/Zn-SOD is primarily used as an antioxidant marker. Levels o... Cu/Zn-superoxide dismutase (Cu/Zn-SOD) is an enzyme that is ubiquitously present in the cytoplasm and causes dismutation of superoxide radicals, therefore Cu/Zn-SOD is primarily used as an antioxidant marker. Levels of Cu/Zn-SOD are higher in the serum of hemodialysis patients than in serum of healthy volunteers. The increase of serum Cu/Zn-SOD levels is related to the decrease of kidney function with aging and arteriosclerosis in hemodialysis patients. Moreover, infection, vascular puncture, and hemostasis may be related to the increase in serum Cu/Zn-SOD levels. As it is associated with numerous factors in hemodialysis patients, Cu/Zn-SOD may serve as a complex marker for arteriosclerosis, vascular, and inflammatory conditions. It is important to investigate various agents that decrease serum Cu/Zn-SOD levels to improve the life-span of hemodialysis patients. 展开更多
关键词 cu/zn-superoxide dismutase HEMODIALYSIS Complex MARKER
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Hydrogen promotes the activation of Cu, Zn superoxide dismutase in a rat corneal alkali-burn model
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作者 Takeshi Arima Tsutomu Igarashi +4 位作者 Masaaki Uchiyama Maika Kobayashi Ikuroh Ohsawa Akira Shimizu Hiroshi Takahashi 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2020年第8期1173-1179,共7页
AIM: To investigate the effects of hydrogen(H2) on Cu, Zn superoxide dismutase(SOD1) activation in a rat model of corneal alkali burn. METHODS: In each rat, one cornea was subjected to alkali exposure. Physiological s... AIM: To investigate the effects of hydrogen(H2) on Cu, Zn superoxide dismutase(SOD1) activation in a rat model of corneal alkali burn. METHODS: In each rat, one cornea was subjected to alkali exposure. Physiological saline(saline group) or H2-dissolved saline(H2 group) was instilled continuously on the cornea for 5 min before and after alkali exposure. Inflammatory cells, neovascularization, and cytoplasmic SOD1 levels were evaluated immunohistochemically in enucleated eyes from both groups. Three-dimensional ultrastructural tissue changes in the eyes were analyzed using low-vacuum scanning electron microscopy.RESULTS: The numbers of both inflammatory and vascular endothelial cells were significantly reduced in the corneas of the H2 group(P<0.01). Furthermore, H2 treatment increased both cytoplasmic SOD1 levels(P<0.01) and activity in corneal epithelial cells(P<0.01). Notably, the SOD1 activity level in the H2 group was approximately 2.5-fold greater than that in the saline group.CONCLUSION: H2 treatment suppresses inflammation and neovascularization in the injured cornea and indirectly suppresses oxidative insult to the cornea by upregulating the SOD1 enzyme protein level and activity. 展开更多
关键词 HYDROGEN alkali burn cu Zn superoxide dismutase low-vacuum scanning electron microscopy rats
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hPP10-Cu,Zn-SOD融合蛋白的制备、穿膜效应及其抗氧化、抗炎症功效
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作者 张洁 姚君妍 +4 位作者 杨英桂 王飞 郑清友 李欣 柳长柏 《南方医科大学学报》 CAS CSCD 北大核心 2024年第6期1059-1069,共11页
目的 探究人源性细胞膜穿透肽hPP10携带人源性抗氧化蛋白Cu,Zn-SOD的穿膜效应并观察其抗氧化、抗炎功效。方法利用RT-PCR技术扩增人源性SOD基因片段、酶切连接法构建重组质粒pET15b-Cu,Zn-SOD,pET15b-hPP10-Cu,Zn-SOD;利用镍柱亲和层析... 目的 探究人源性细胞膜穿透肽hPP10携带人源性抗氧化蛋白Cu,Zn-SOD的穿膜效应并观察其抗氧化、抗炎功效。方法利用RT-PCR技术扩增人源性SOD基因片段、酶切连接法构建重组质粒pET15b-Cu,Zn-SOD,pET15b-hPP10-Cu,Zn-SOD;利用镍柱亲和层析法、分子筛方法纯化Cu,Zn-SOD、hPP10-Cu,Zn-SOD融合蛋白并利用Western blotting法鉴定其正确性;利用免疫荧光法、荧光共定位实验、Western blotting法鉴定hPP10-Cu,Zn-SOD在细胞中的穿膜效应;利用Western blotting法鉴定hPP10-Cu,Zn-SOD融合蛋白穿膜时间梯度和浓度梯度效应;利用SOD酶活性测定试剂盒检测hPP10-Cu,Zn-SOD穿膜后的SOD酶活性;利用MTT法检测hPP10对细胞活性的影响。利用H2O2建立HEK293氧化应激细胞模型,通过流式细胞分析术(FCM)分析hPP10-Cu,Zn-SOD穿膜后细胞凋亡情况;并RT-qPCR分析hPP10-Cu,Zn-SOD穿膜后凋亡相关因子的表达情况;通过ROS检测分析hPP10-Cu,Zn-SOD穿膜后抗氧化能力。TPA诱导建立小鼠耳部炎症模型(5只/组,共4组),然后RT-qPCR分析hPP10-Cu,Zn-SOD穿膜后,NFκB,IL-1β、IL-6、TNFα因子的转录情况;Western blotting检测NFκB p65、p-NFκB p65、IL-1β、TNFα基因的表达;免疫组化分析炎性因子p-NFκB p65、TNFα基因的表达。结果 成功构建了重组质粒pET15b-Cu,Zn-SOD,pET15b-hPP10-Cu,Zn-SOD,并获得Cu,Zn-SOD蛋白和hPP10-Cu,Zn-SOD融合蛋白;免疫荧光试验结果表明5μmol/L浓度的hPP10-Cu,Zn-SOD转导HEK293细胞具有明显的穿膜效应;荧光共定位实验显示融合蛋白hPP10-Cu,Zn-SOD入胞后定位在细胞膜内,部分蛋白定位在细胞核内;Western blotting实验结果表明hPP10-Cu,Zn-SOD转导Hela(P<0.05),HEK293(P<0.01)细胞具有浓度依赖性,细胞内hPP10-Cu,Zn-SOD存在可持续24 h;5μmol/L的hPP10-Cu,Zn-SOD转导细胞具有较好的抗氧化活性(P<0.01);MTT结果显示高达10μmol/L浓度的hPP10-Cu,Zn-SOD对于细胞活力的影响小。在氧化应激模型中,FCM结果显示hPP10-Cu,Zn-SOD融合蛋白孵育细胞降低了细胞的早期凋亡(P<0.01)和晚期凋亡(P<0.05);RT-qPCR结果显示hPP10-Cu,Zn-SOD融合蛋白孵育细胞使Bcl2、Mcl1、Deptor等抗凋亡因子升高(P<0.05),降低了Bad、P21、P27等促凋亡因子的表达(P<0.05);ROS结果显示hPP10-Cu,Zn-SOD融合蛋白孵育细胞显著降低了细胞内的活性氧含量(P<0.01)。在炎症模型中,hPP10-Cu,Zn-SOD融合蛋白可显著降低IL-1β、IL-6、TNFα因子的转录(P<0.05);p-NFκB p65、IL-1β及TNFα的表达都呈现了抑制作用(P<0.05);hPP10-Cu,Zn-SOD融合蛋白处理组较Cu,Zn-SOD蛋白处理组,降低了炎性因子p-NFκB p65及TNFα基因的表达(P<0.05)。结论 融合蛋白hPP10-Cu,Zn-SOD具有明显的穿膜入胞、抗氧化、抗炎功效,且对细胞毒副作用很小。这些结果为hPP10作为新的递送载体奠定基础,也为hPP10-Cu,Zn-SOD应用于护肤品等提供了理论依据。 展开更多
关键词 人源性细胞膜穿透肽hPP10 铜锌超氧化物歧化酶cu Zn-SOD 抗氧化 抗炎 酶活力测定
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Partial characterization of superoxide dismutase activity in the Barber pole worm-Haemonchus contortus infecting Capra hircus and abomasal tissue extracts
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作者 Sadia Rashid Malik Irshadullah 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第9期718-724,共7页
Objective:To determine the activity of superoxide dismutase(SOD) in the male and female haematophagous caprine worms,Haemonchus contortus infecting Capra hircus,and their E/S products and also to analyse the effect of... Objective:To determine the activity of superoxide dismutase(SOD) in the male and female haematophagous caprine worms,Haemonchus contortus infecting Capra hircus,and their E/S products and also to analyse the effect of Haemonchus infection on the level of host SOD.Methods:The SOD activity was analysed by using the pyrogallol autoxidation assay and non-denaturing polyacrylamide gel electrophoresis followed by specific enzyme staining by riboflavin-nitroblue tetrazolium method.Results:The adult females were found to have higher enzyme activity than the male worms.Appreciable amount of SOD activity was also detected in the worm culture medium and female worms secreted more SOD in comparison to the male parasites.The SOD activity was negatively correlated to the worm burden.Statistically significant decrease in SOD activity(P<0.05) was observed in the heavily infected host tissue in comparison to the control non-infected host tissue.SOD profile of the crude extracts of both the sexes revealed polymorphism and a fast migrating activity band being characteristic of E/S products.The SOD activities were found highly sensitive to potassium cyanide indicating the Cu/Zn form of SOD.Conclusions:Haemonchus contortus is a key model parasite for drug and vaccine discovery.The presences of SOD activity in appreciable amount in the parasite as well as its E/S products indicate that it has a well-developed active antioxidant system to protect itself from the host immune attack.SOD could be the target for vaccine development which is the need of the hour as mass drug administration for parasite control has resulted in anthelmintic resistance across the globe and threatens the viability of sheep and goat industry in many regions of the world.The infection with Haemonchus causes a drastic reduction in SOD activity of the host tissue thus effecting its protective potential.One characteristic SOD band was found in the females which was not present in any other preparations and thus could be exploited for further studies on diagnostic/control measures. 展开更多
关键词 Superoxide dismutase HAEMONCHUS contortus POLYACRYLAMIDE gel ELECTROPHORESIS cu/ZN 3-D densitogram
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Inhibition effect of expression of Cu/Zn superoxide dismutase from rice on synthesis of Glutathione in Saccharomyces cerevisiae
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作者 艾玉卓 都业杰 +1 位作者 祖元刚 安志刚 《Journal of Forestry Research》 SCIE CAS CSCD 2008年第1期63-66,共4页
The expression of a rice Cu/Zn superoxide dismutase (Cu/Zn-SOD) in Saccharomyces cerevisiae regulated by GAPDH promoter, involved in the inhibition of endogenous Glutathione (GSH) synthesis, and the competitive ex... The expression of a rice Cu/Zn superoxide dismutase (Cu/Zn-SOD) in Saccharomyces cerevisiae regulated by GAPDH promoter, involved in the inhibition of endogenous Glutathione (GSH) synthesis, and the competitive expression was detected by constructing the expression vector transferred Cu/Zn-SOD gene into wild-type S. cerevisiae. Transcription and expression of the Cu/Zn-SOD gene in S. cerevisiawere were confirmed by northern blot and SDS-PAGE, respectively, and activity of the Cu/Zn-SOD from crude extracts was enzymatically detected based on the effect of nitroblue tetrazolium (NBT) after running a native polyacrylamide gel. The GSH synthesis was also tested by DTNB (5, 5'-Dithiobis (2-nitrobenzoic acid)) method. Results showed that GSH synthesis was evidently suppressed by the expression of Cu/Zn-SOD gene in both control and heat shock strains. It implied that the expression of the Cu/Zn-SOD gene in S. cerevisiae has more potential facility in response to oxidative exposure than that of endogenous GSH, although Cu/Zn-SOD and GSH were both contributed to the function of oxygen radical oxidoreduction. 展开更多
关键词 cu/Zn Superoxide dismutase GAPDH promoter Saccharomyces cerevisiae GLUTATHIONE
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高负载Cu单原子纳米酶的制备和类酶活性研究
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作者 陈心珠 刘晓宇 +2 位作者 张少芳 穆晓宇 张晓东 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2023年第11期2684-2696,共13页
目的 单原子纳米酶(single-atom nanozyme,SAN)因其高原子利用率及丰富的类酶活性被广泛研究。但是目前大多数SAN活性位点负载量较低,限制了其进一步应用和发展。本研究旨在制备一种高原子负载量的SAN,并对其类酶活性进行系统研究,希望... 目的 单原子纳米酶(single-atom nanozyme,SAN)因其高原子利用率及丰富的类酶活性被广泛研究。但是目前大多数SAN活性位点负载量较低,限制了其进一步应用和发展。本研究旨在制备一种高原子负载量的SAN,并对其类酶活性进行系统研究,希望为高负载SAN的制备提供思路,并为SAN在更广泛领域的应用提供理论支持。方法 本研究通过原位锚定策略将金属盐前驱体锚定在氨基化石墨烯量子点框架中,在惰性气体保护下进行高温热解稳定Cu原子和载体之间的化学键,制备出负载量高达7.66%(质量百分比)的高负载Cu单原子纳米酶(high-loading Cu SAN)。此外,以3,3’,5,5’-四甲基联苯胺(TMB)和氮蓝四唑(NBT)为显色剂,评估了high-loading Cu SAN的类过氧化物酶(POD)、类氧化物酶(OXD)及类超氧化物歧化酶(SOD)活性,并与传统金属有机框架锚定法制备的低负载Cu单原子纳米酶(low-loading Cu SAN)作比较。以过氧化氢(H_(2)O_(2))为催化底物,对比研究了高/低负载Cu SAN的类过氧化氢酶(CAT)活性。结果 研究表明,本文制备的高负载Cu SAN的类POD和SOD活性分别是低负载Cu SAN的3.4倍和8.88倍,且表现出类酶催化选择性。结论 本研究为高负载SAN的制备和活性研究提供了思路,为SAN在检测传感、疾病治疗以及环境保护等方面的应用奠定了基础。 展开更多
关键词 单原子纳米酶 cu原子 高负载 类过氧化物酶活性 类超氧化物歧化酶活性
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Does wild-type Cu/Zn-superoxide dismutase have pathogenic roles in amyotrophic lateral sclerosis? 被引量:1
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作者 Yoshiaki Furukawa Eichi Tokuda 《Translational Neurodegeneration》 SCIE CAS 2020年第3期421-436,共16页
Amyotrophic lateral sclerosis(ALS)is characterized by adult-onset progressive degeneration of upper and lower motor neurons.Increasing numbers of genes are found to be associated with ALS;among those,the first identif... Amyotrophic lateral sclerosis(ALS)is characterized by adult-onset progressive degeneration of upper and lower motor neurons.Increasing numbers of genes are found to be associated with ALS;among those,the first identified gene,SOD1 coding a Cu/Zn-superoxide dismutase protein(SOD1),has been regarded as the gold standard in the research on a pathomechanism of ALS.Abnormal accumulation of misfolded SOD1 in affected spinal motor neurons has been established as a pathological hallmark of ALS caused by mutations in SOD1(SOD1-ALS).Nonetheless,the involvement of wild-type SOD1 remains quite controversial in the pathology of ALS with no SOD1 mutations(non-SOD1 ALS),which occupies more than 90%of total ALS cases.In vitro studies have revealed post-translationally controlled misfolding and aggregation of wild-type as well as of mutant SOD1 proteins;therefore,SOD1 proteins could be a therapeutic target not only in SOD1-ALS but also in more prevailing cases,non-SOD1 ALS.In order to search for evidence on misfolding and aggregation of wild-type SOD1 in vivo,we reviewed pathological studies using mouse models and patients and then summarized arguments for and against possible involvement of wild-type SOD1 in non-SOD1 ALS as well as in SOD1-ALS. 展开更多
关键词 Amyotrophic lateral sclerosis cu/zn-superoxide dismutase Protein misfolding
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Substituent Effect on Proton Affinity of Imidazole in Cu,Zn-Superoxide Dismutase
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作者 纪洪芳 张红雨 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2006年第6期822-824,共3页
To investigate whether the proton-accepting ability of imidazole in Cu,Zn-superoxide dismutase (SOD) was possibly modulated by Zn(Ⅱ) or not, the proton affinity (Ap) of N^3 in imidazole group was calculated by ... To investigate whether the proton-accepting ability of imidazole in Cu,Zn-superoxide dismutase (SOD) was possibly modulated by Zn(Ⅱ) or not, the proton affinity (Ap) of N^3 in imidazole group was calculated by density functional theory (DFT) with B3LYP functional. It was found that Zn(Ⅱ) attenuates the Ap, because of its electron-withdrawing effect, while the three ligands connected with Zn(Ⅱ) (residues of two His and one Asp) exert an opposite effect, owing to their electron-donating ability. This finding suggested that the three ligands should play a role in the normal function of Cu,Zn-SOD and should be taken into consideration in the future study. 展开更多
关键词 cu zn-superoxide dismutase proton affinity density functional theory substituent effect
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妇科肿瘤血清Cu、Zn及CuZn-SOD活力的检测
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作者 赵锐 刘雁飞 +2 位作者 刘絮 梁铁彪 王晓玲 《世界元素医学》 2005年第4期27-28,共2页
目的:本研究探讨妇科肿瘤患者血清Cu、Zn及CuZn-SOD活力的变化及其意义。方法:采用原子吸收分光光度法和黄嘌呤氧化酶法测定患者与正常对照组血清Cu、Zn及CuZn-SOD的活力。结果:妇科良、恶性肿瘤血清Cu含量均高于正常对照组,妇科恶... 目的:本研究探讨妇科肿瘤患者血清Cu、Zn及CuZn-SOD活力的变化及其意义。方法:采用原子吸收分光光度法和黄嘌呤氧化酶法测定患者与正常对照组血清Cu、Zn及CuZn-SOD的活力。结果:妇科良、恶性肿瘤血清Cu含量均高于正常对照组,妇科恶性肿瘤血清Cu高于妇科良性肿瘤。妇科良恶性肿瘤Zn、CuZn-SOD活力均低于正常组,妇科恶性肿瘤血清Zn、CuZn-SOD活力低于妇科良性肿瘤。结论:妇科肿瘤的发生发展与微量元素、自由基紊乱有关。 展开更多
关键词 妇科肿瘤cu ZN cuZN-SOD 自由基
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杨梅Cu/Zn超氧化物歧化酶基因(MrSOD1)cDNA的克隆及表达分析 被引量:12
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作者 王芳 董乐 +2 位作者 戴聪杰 林娈 林静 《中国农学通报》 CSCD 北大核心 2010年第22期27-33,共7页
以杨梅叶为材料,利用RT-PCR技术克隆Cu/Zn超氧化物歧化酶基因,并利用半定量RT-PCR方法分析该基因在不同组织中的表达。获得1个杨梅Cu/ZnSOD基因编码区全长cDNA序列,长度为461bp,编码一个由152个氨基酸残基组成的蛋白质。该蛋白质具有Cu/... 以杨梅叶为材料,利用RT-PCR技术克隆Cu/Zn超氧化物歧化酶基因,并利用半定量RT-PCR方法分析该基因在不同组织中的表达。获得1个杨梅Cu/ZnSOD基因编码区全长cDNA序列,长度为461bp,编码一个由152个氨基酸残基组成的蛋白质。该蛋白质具有Cu/ZnSOD的特征信号序列;序列同源性分析表明,其与GenBank中注册的Cu/ZnSOD序列的同源性均在77%以上。该基因命名为MrSOD1(GenBank登录号:GQ404510)。半定量RT-PCR分析显示,MrSOD1在4种组织中表达量为:果>叶>花>枝条。本试验为进一步研究MrSOD1的时空表达特性、调控途径及杨梅抗氧化伤害的分子机理奠定基础。 展开更多
关键词 杨梅 cu/Zn超氧化物歧化酶 基因克隆 组织表达
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Cu^(2+)对中华倒刺鲃超氧化物歧化酶活性的影响 被引量:11
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作者 孙翰昌 丁诗华 +2 位作者 代梅 黄丽英 耿晓修 《西南农业大学学报(自然科学版)》 CSCD 北大核心 2005年第5期709-712,共4页
Cu2+是养殖水体的常见污染物,为了评估Cu2+对鱼类超氧化物歧化酶(SOD)的影响,将中华倒刺鲃暴露于含0.01~0.32 mg/L Cu2+的水体中饲养8 d,测定肌肉、血浆及肝脏中SOD活性的变化。实验结果显示,低浓度Cu2+(0.01 mg/L)对肌肉、血浆及肝脏的... Cu2+是养殖水体的常见污染物,为了评估Cu2+对鱼类超氧化物歧化酶(SOD)的影响,将中华倒刺鲃暴露于含0.01~0.32 mg/L Cu2+的水体中饲养8 d,测定肌肉、血浆及肝脏中SOD活性的变化。实验结果显示,低浓度Cu2+(0.01 mg/L)对肌肉、血浆及肝脏的SOD有诱导作用,随着处理时间的延长而升高。但当浓度较高(0.04 mg/L以上)时,SOD活性均呈现先诱导后抑制的趋势。Cu2+浓度越高,SOD活性上升越急剧,出现活性抑制的时间就越提前。此外,SOD在3种组织中的分布亦存在明显差异,肝脏的SOD活性最大。这些结果表明,肝脏SOD活性更宜作为Cu2+污染的生物标记。 展开更多
关键词 中华倒刺妃 超氧化物歧化酶 cu^2+
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盐胁迫下花花柴 miR398 对 Cu/Zn 超氧化物歧化酶基因的调控研究 被引量:5
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作者 杜驰 廖茂森 +1 位作者 张霞 张富春 《西北植物学报》 CAS CSCD 北大核心 2014年第4期682-688,共7页
植物miRNA能够参与盐胁迫下基因表达的调控。在盐胁迫条件下盐生植物花花柴miR398(KcmiR398)呈现显著性差异表达,为了探讨rniR398的靶基因及其对靶基因的调控方式,通过生物信息学分析(http://plant—grn.noble.Org/psRNATar... 植物miRNA能够参与盐胁迫下基因表达的调控。在盐胁迫条件下盐生植物花花柴miR398(KcmiR398)呈现显著性差异表达,为了探讨rniR398的靶基因及其对靶基因的调控方式,通过生物信息学分析(http://plant—grn.noble.Org/psRNATarget/),预测KcmiR398的靶基因分别为Cu/Zn超氧化物歧化酶(SOD)基因CSDl和CSD2。利用RACE技术从花花柴中克隆到CSDl和CSD2基因全长序列,分别命名为KcCSDl和KcCSD2。qRTPCR结果表明:(1)KcmiR398在盐胁迫的花花柴茎中上调表达,在新叶中下调表达;KcCSDl在盐胁迫的老叶中上调表达,而在老茎和根中下调表达;KcCSD2在盐胁迫的老茎和根中上调表达。(2)300mmol/I,NaCl胁迫24~48h,KcmiR398与对照无显著差异;KcCSDl的表达为对照的3倍以上,而KcCSD2的表达没有显著差异。研究表明,花花柴的KcmiR398和KcCSDl、KcCSD2有组织差异性表达,盐胁迫可以影响KcmiR398和KcCSDl、KcCSD2基因的表达以适应盐胁迫产生的氧化危害。 展开更多
关键词 花花柴 盐胁迫 miR398 cu/Zn超氧化物歧化酶 表达分析
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Cu^(2+)离子对水丝蚓的急性毒性及超氧化物歧化酶活性的影响 被引量:8
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作者 柳郁滨 范学铭 王哲娟 《中国农学通报》 CSCD 北大核心 2010年第19期423-425,共3页
采用急性毒性方法,测定了不同质量分数的Cu2+离子溶液处理后的水丝蚓48h半致死浓度(LC50)为8.640μL/L;用测定酶活性方法检测了中毒后的水丝蚓的超氧化物歧化酶(SOD)活性的变化,与对照组比较,Cu2+离子对动物SOD活性影响显著(P<0.05)... 采用急性毒性方法,测定了不同质量分数的Cu2+离子溶液处理后的水丝蚓48h半致死浓度(LC50)为8.640μL/L;用测定酶活性方法检测了中毒后的水丝蚓的超氧化物歧化酶(SOD)活性的变化,与对照组比较,Cu2+离子对动物SOD活性影响显著(P<0.05)。低浓度药物(0.500~5.340)μL/L处理动物,SOD活性呈上升趋势;当浓度增加至11.740μL/L时,SOD活性开始表现为不同程度的降低。说明Cu2+离子对水丝蚓造成胁迫,直至动物个体死亡,间接或直接地影响水生生态系统及以水丝蚓为食物的下一级消费者。 展开更多
关键词 克拉伯水丝蚓 cu2+离子 半致死浓度(LC50) 超氧化物歧化酶(SOD)
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Cu^(2+)、Cd^(2+)胁迫对小麦幼根SOD活性及其基因表达的影响 被引量:8
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作者 李春喜 张志娟 +3 位作者 张黛静 邵云 姜丽娜 李婷婷 《中国农业科技导报》 CAS CSCD 2011年第4期92-98,共7页
采用水培试验探索不同浓度(0 mg/L、5 mg/L、30 mg/L和60 mg/L)Cu2+或Cd2+胁迫(24 h、48 h、72 h和96 h)后对小麦幼根超氧化物歧化酶(SOD)活性的影响,并通过实时荧光定量聚合酶链式反应(real-timefluorescence quantitative PCR,Real-ti... 采用水培试验探索不同浓度(0 mg/L、5 mg/L、30 mg/L和60 mg/L)Cu2+或Cd2+胁迫(24 h、48 h、72 h和96 h)后对小麦幼根超氧化物歧化酶(SOD)活性的影响,并通过实时荧光定量聚合酶链式反应(real-timefluorescence quantitative PCR,Real-time Q-PCR)检测Cu/ZnSOD基因表达。结果表明,Cu2+胁迫处理72 h后,各处理SOD活性均较对照极显著提高(P<0.01),且30 mg/L浓度下SOD酶活性最高,是对照的3.67倍;而Cd2+胁迫处理后,幼根SOD活性整体呈现"升-降-升"的趋势,胁迫前期酶活性的降低程度与Cd2+浓度成正比,至72 h酶活性最低。Cd2+胁迫条件下Cu/ZnSOD的表达量明显高于Cu2+胁迫。不同时间Cu2+胁迫下,Cu/ZnSOD基因表达均呈现5 mg/L浓度处理高于60 mg/L和30 mg/L,表明低浓度促进而高浓度抑制该基因表达;而对于Cd2+胁迫,Cu/ZnSOD基因随浓度的升高和时间的延长呈现明显下降趋势,至96 h最低。本实验结果表明,Cu2+、Cd2+胁迫处理后浓度对基因表达的影响较大。该结果为深入探讨重金属胁迫下小麦幼根中SOD特性的研究提供理论参考和技术支持。 展开更多
关键词 小麦 cu2+和Cd2+胁迫 超氧化物歧化酶 Real-timeQ-PCR 基因表达
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Cu^(2+)、Zn^(2+)对罗氏沼虾鳃中SOD同工酶的影响 被引量:7
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作者 俞亚东 许燕 杨洁 《上海师范大学学报(自然科学版)》 2009年第2期189-192,共4页
采用生态学单因子梯度试验方法,研究了水环境中添加0.01mg/L,0.08mg/L,0.64mg/L,5.12mg/L的Cu2+,Zn2+对罗氏沼虾鳃中超氧化物歧化酶(SOD)同工酶活力和酶谱的影响.结果显示:随着Cu2+,Zn2+浓度的增加鳃中SOD酶活力均呈先增加后减少的变化... 采用生态学单因子梯度试验方法,研究了水环境中添加0.01mg/L,0.08mg/L,0.64mg/L,5.12mg/L的Cu2+,Zn2+对罗氏沼虾鳃中超氧化物歧化酶(SOD)同工酶活力和酶谱的影响.结果显示:随着Cu2+,Zn2+浓度的增加鳃中SOD酶活力均呈先增加后减少的变化趋势,分别在0.64 mg/L Cu2+和0.08 mg/L Zn2+时SOD酶活力达到最大(73.57±0.12 U/mg和53.63±0.17 U/mg),且均与对照组有显著差异(P<0.05);低浓度Cu2+处理组SOD同工酶酶谱出现SOD酶带变宽、颜色加深现象,并在0.08 mg/L和0.64 mg/L浓度组中新增一条酶带(SOD2),而高浓度组(5.12mg/L)明显抑制SOD酶带活性,新增的条带也缺失;同样低浓度的Zn2+使得SOD酶谱的条带变宽,颜色加深,但没有出现新增酶带,而高浓度(5.12 mg/L)Zn2+使得条带变窄,颜色变淡.结果表明:这两种金属离子对罗氏沼虾鳃中SOD同工酶活力及酶谱的影响与浓度有关,Cu2+对SOD的影响明显大于Zn2+,SOD同工酶活力和酶谱的变化可以灵敏地反映Cu2+的胁迫程度和毒性. 展开更多
关键词 罗氏沼虾 cu2+ Zn2+ 同工酶 超氧化物歧化酶
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透析法和稀释法复性重组人Cu,Zn-SOD包含体 被引量:1
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作者 刘建荣 赵晓瑜 +2 位作者 王之宇 刘建国 静天玉 《河北大学学报(自然科学版)》 CAS 北大核心 2006年第4期411-414,419,共5页
以稀释法和透析法对在E.coli中以包含体形式表达的重组人超氧化物歧化酶(rhSOD)进行复性.以8 mol/L尿素溶解分离纯化的包含体(纯度达80%以上),对其稀释或透析至尿素终浓度为1.0 mol/L后,在稀释样品中补加终浓度50μmoL/L的Cu2+和5.0μmo... 以稀释法和透析法对在E.coli中以包含体形式表达的重组人超氧化物歧化酶(rhSOD)进行复性.以8 mol/L尿素溶解分离纯化的包含体(纯度达80%以上),对其稀释或透析至尿素终浓度为1.0 mol/L后,在稀释样品中补加终浓度50μmoL/L的Cu2+和5.0μmoL/L的Zn2+,在透析样品中补加终浓度5.0μmoL/L的Cu2+和0.5μmoL/L的Zn2+,分别获得了比活3 300 u/mg和4 300 u/mg的复性rhSOD.该复性样品经铜螯合亲和层析柱纯化可获得更高比活性的rhSOD. 展开更多
关键词 重组人cu Zn—SOD 复性 包含体
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葡萄糖和Cu^(2+)对蛹虫草Cu,Zn超氧化物歧化酶在E.coli中表达的影响 被引量:1
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作者 王尊生 何卓晶 +1 位作者 宋聿文 袁勤生 《中国医药工业杂志》 CAS CSCD 北大核心 2006年第8期522-524,共3页
对含有蛹虫草Cu,Zn-超氧化物歧化酶(cm-SOD)基因的重组E.coli培养条件进行了初步优化。结果表明,LB培养基国含有Cu^(2+)可明显提高超氧化物歧化酶比活力;而在菌体培养中补加葡萄糖,可提高重组蛋白表达量和细胞生物量。在含有0.6mmol/... 对含有蛹虫草Cu,Zn-超氧化物歧化酶(cm-SOD)基因的重组E.coli培养条件进行了初步优化。结果表明,LB培养基国含有Cu^(2+)可明显提高超氧化物歧化酶比活力;而在菌体培养中补加葡萄糖,可提高重组蛋白表达量和细胞生物量。在含有0.6mmol/L Cu^(2+)、Zn^(2+)的LB培养基中补加22mmol/L葡萄糖,每200ml培养液可获得lg湿细胞,超氧化物歧化酶比活力达到3305u/mg。 展开更多
关键词 cu Zn-超氧化物歧化酶 蛹虫草 表达
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miRNA398调控烟草Cu/Zn-SOD基因的表达 被引量:2
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作者 周小龙 魏春阳 +8 位作者 黄佳 史艳梅 王皓 罗朝鹏 李锋 王燃 杨军 陈继峰 林福呈 《烟草科技》 EI CAS 北大核心 2014年第4期99-102,共4页
模式植物miRNA398可以抑制靶基因Cu/Zn-SOD的表达。为揭示烟草miRNA398的生物学功能,采用荧光定量PCR技术分析了烟草品种CB-1和B37在干旱胁迫条件下miRNA398和Cu/Zn-SOD基因的表达量,并预测了烟草miRNA398结合Cu/Zn-SOD基因的位点。结... 模式植物miRNA398可以抑制靶基因Cu/Zn-SOD的表达。为揭示烟草miRNA398的生物学功能,采用荧光定量PCR技术分析了烟草品种CB-1和B37在干旱胁迫条件下miRNA398和Cu/Zn-SOD基因的表达量,并预测了烟草miRNA398结合Cu/Zn-SOD基因的位点。结果表明:烟草miRNA398与Cu/Zn-SOD基因5非翻译区的21个碱基序列互补,从而抑制该基因的表达;在干旱胁迫条件下,CB-1的miRNA398表达水平上升,B37的miRNA398表达水平下降,Cu/Zn-SOD基因表现出与miRNA398相反的表达趋势,说明抗旱能力存在差异的两个品种中miRNA398均可抑制Cu/Zn-SOD基因表达,并且对干旱胁迫响应具有两种不同的方式。 展开更多
关键词 烟草 miRNA398 cu Zn-SOD基因
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Cu^(2+)对罗氏沼虾体内SOD酶细胞化学定位的影响 被引量:2
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作者 杨洁 许燕 俞亚东 《上海师范大学学报(自然科学版)》 2009年第4期418-422,共5页
运用电镜酶细胞化学技术显示,罗氏沼虾经高浓度Cu2+(5.12 mg/L)处理一周后,体内3种组织(鳃、肝胰腺和心脏)细胞超微结构均有不同程度的损伤,主要表现为细胞核收缩,线粒体膨胀和内嵴减少,细胞结构模糊、胞质浑浊,髓样小体增多,脂滴破裂等... 运用电镜酶细胞化学技术显示,罗氏沼虾经高浓度Cu2+(5.12 mg/L)处理一周后,体内3种组织(鳃、肝胰腺和心脏)细胞超微结构均有不同程度的损伤,主要表现为细胞核收缩,线粒体膨胀和内嵴减少,细胞结构模糊、胞质浑浊,髓样小体增多,脂滴破裂等;超氧化物歧化酶(SOD)阳性颗粒在健康虾中主要存在于细胞核、线粒体膜周围和脂滴周围等,Cu2+胁迫后,组织细胞中线粒体膜和脂滴周围的SOD阳性颗粒明显减少,细胞核内SOD阳性颗粒缺失.结果表明:高浓度Cu2+对罗氏沼虾体内3种组织细胞中SOD的表达具有显著抑制作用,可导致虾体免疫机能显著下降,从而出现组织细胞结构明显损伤. 展开更多
关键词 罗氏沼虾 cu2+ 超氧化物歧化酶(SOD)
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Effect of Excipients on Stability and Structure of rhCuZn-SOD Encapsulated in PLGA Microspheres
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作者 LIU Ling 1,2 ,GE Yu 1 and YUAN Qin-sheng 1 1. State Key Laboratory of Bioreactor Engineering and Institute of Biochemistry,East China University of Science and Technology,Shanghai 200237,P. R. China 2. Public Health School,Nanjing Medical University,Nanjing 210029,P. R. China 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2004年第3期323-327,共5页
When a protein is encapsulated into poly( DL -lactide-co-glycolide)(PLGA) microspheres by means of the double-emulsion method,the harsh microspheres formation process including ultrasonification,exposure to an organic... When a protein is encapsulated into poly( DL -lactide-co-glycolide)(PLGA) microspheres by means of the double-emulsion method,the harsh microspheres formation process including ultrasonification,exposure to an organic solvent and a polymer may cause the denaturation of the protein. In this study,we investigated the enzymatic activity change and the effect of the excipients on the stability of recombinant human Cu,Zn-superoxide dismutase(rhCu,Zn-SOD) during the emulsification. The specific activity recovery was found to be concentration dependent and the excipients involved such as PEG 600 and Tween 20,and trehalose were shown to increase the stability of rhCu,Zn-SOD. The protein structural integrity within the microspheres was analyzed by FTIR. The structure of rhCu,Zn-SOD within PLGA microspheres containing trehalose was found to be similar to that of the native solid state,whereas the protein encapsulated during the preparation in the absence of any excipient changed due to the possible hydrophobic interaction with the polymer. The results suggest that a rational stability strategy for protein to be encapsulated into microspheres should aim at different processes. 展开更多
关键词 Poly( DL -lactide-co-glycolide)(PLGA) microsphere Recombinant human cu zn-superoxide dismutase(rhcu Zn-SOD) Fourier transform infrared(FTIR) spectroscopy Protein stability EXCIPIENT
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