P450(cytochrome P450)is a supergene family,which is involved in various metabolic pathways in plants.Based on previous study,we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts.However...P450(cytochrome P450)is a supergene family,which is involved in various metabolic pathways in plants.Based on previous study,we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts.However,the reason that why P450 mRNAs were endorsed as signaling,and what specific motif(s)did they harbored is not clear yet.Here,we first identified 221 CsaP450 genes in cucumber genomewide level.Combining with graft-transmissiblemRNAs datasets in cucumber,we elucidated 15mobile-CsaP450-coding genes,of which 5 and 10 belonged to A-type and non-A type respectively.Compared with Arabidopsis and pumpkin(Cucubit moschata)graft-induced-transmissible P450 mRNAs,a phylogenetic treewas constructed and divided into eight clans by usingmultiple-sequence alignment.Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways.The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes.The integrated analysis of mobility direction and mRNA abundance of 15 mobilemRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them.The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.展开更多
In the entire world, about 200 million populations are exposed to arsenic poisoning in groundwater. Arsenic is a very poisonous metalloid and has three allotropic forms. The toxic inorganic arsenic is converted and ut...In the entire world, about 200 million populations are exposed to arsenic poisoning in groundwater. Arsenic is a very poisonous metalloid and has three allotropic forms. The toxic inorganic arsenic is converted and utilized metabolically into organic form. It is primarily eliminated out from the human body through metabolic wastes like urine and is also deposited in the hair, nails and skin of the affected ones. Arsenic is known to be a carcinogen as Carcinogen category 1 in the forms of arsenic trioxide, arsenic pentoxide, arsenous acids, arsenic acid and their salts. The arsenic also affects the epidermal system, the nervous system and the vascular system of humans. In the present investigation for toxicological evaluation of arsenic Liver Function Tests & Kidney Function Tests have been considered. It is presumed that medicinal plants have significance in the present day in view of the lethal diseases like AIDS, cancer, hepatitis, nephritis sterility too for which no effective drugs are found till today in modern system of medicine. Medicinal plant based drugs generally have no side effects or the least side effect. Thus to evaluate an ameliorating effect, if any, to mitigate the arsenic toxicity in mice, for which Cucumis sativus (cucumbers) has been taken as curative measure. Sodium arsenite at the dose of 3 mg/kg body weight was administered for 4 weeks followed by the administration of Cucumis sativus (cucumbers) for 4 and 6 weeks at dose of 500 mg/kg body weight. Their biochemical levels like liver and kidney function tests were assayed and were found with elevated levels. But, after administration of aqueous extract of Cucumis sativus (cucumbers), there was significant amelioration in the biochemical levels. The protective effect of Cucumis sativus (cucumbers) was shown in the form of normalization of enzymatic and non-enzymatic activities represented by normalization of liver and kidney functions.展开更多
Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that...Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that it also exists in the sieve element-companion cell (SE-CC) complex, being widely distributed in P-protein filaments and sieve element reticulum (SER), in the cytoplasm and mitochondrial membrane of companion cell (CC) and in the branched plasmodesmata between sieve element (SE) and CC as well. The results suggest that this protein could be synthesized in CC and transferred to SE through plasmodesmata. Western blotting showed that spectrin-like protein existed in the protein of phloem exudate of cucumber, and its molecular weight was about 260 kD.展开更多
Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplifi...Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplified fragment length polymorphism （AFLP） markers are studied to formulate efficient strategies for breeding cultivars resistant to powdery mildew. The joint analysis of multiple generations and AFLP technique has been applied in this study. The best model is the one with two major genes, additive, dominant, and epistatic effects, plus polygenes with additive, dominant, and epistatic effects （E-l-0 model）. The heritabilities of the major genes varied from 64.26% to 97.82%, and susceptibility was incompletely dominant for the two major genes in the three crosses studied. The additive effects of the two major genes and the dominant effect of the second major gene were high, and the epistatic effect of the additive-dominant between the two major genes was the highest in cross I . In cross II, the absolute value of the additive effect, dominant effect, and potential ratio of the first major gene were far higher than those of the second major gene, and the epistatic effect of the additive-additive was the highest. The genetic parameters of the two major genes in cross III were similar to those in cross II. Correlation and regression analyses showed that marker E25/M63-103 was linked to a susceptible gene controlling powdery mildew resistance. The marker could account for 19.98% of the phenotypic variation. When the marker was tested on a diverse set of 29 cucumber lines, the correlation between phenotype and genotype was not significant, which suggested cultivar specialty of gene expression or different methods of resistance to powdery mildew. The target DNA fragment was 103 bp in length, and only a small part was found to be homologous to DNA in the other species evaluated, which indicated that it was unique to the cucumber genome.展开更多
Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major...Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.展开更多
Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes.
There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on micro...There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on microstructure, most reports are focused on the toxicology rather than promotion. Using cucumis sativus L. (Jin Chun No.5) as experiment material, we try to find out the nutritional effects of low Y3+ concentrations on cucumber seedlings′ leaves. The present paper suggests that the rare earth elements act as micronutrients at low concentrations while they give rise to toxicity at high concentration. Benefits defeat toxicity with concentration ranging from 5 to 25 μmol·L-1. Through careful study, at the Y(NO3)3 concentration of 10 μmol·L-1 the content of chlorophyll as well as the activities of SOD, Cu-Zn SOD and the POD are the highest. It indicates 10 μmol·L-1 is the optimum concentration of yttrium for promoting the cucumber growth.展开更多
An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for furth...An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1 The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively. Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome configurations were observed in the pollen mother cells （PMCs） of hybrid F1 at metaphase Ⅰ , e.g., univalents, bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains （about 23.3%）. Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix x NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus.展开更多
Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma m...Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of plants.Cucumber is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely unknown.Here,we report the functional characteristics of Cs NIP2;2.It was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter gene.Cs NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix position.Xenopus laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low temperature.Cs NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other tissues.Transient expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the cell.The results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other plants.These findings may be helpful for understanding the functions of NIPs in cucumber plants.展开更多
Plant height in cucumber is not only an important trait for breeding,but also one of the model traits suitable for the study of developmental biology.Amplified fragment length polymorphism(AFLP)and simple sequence rep...Plant height in cucumber is not only an important trait for breeding,but also one of the model traits suitable for the study of developmental biology.Amplified fragment length polymorphism(AFLP)and simple sequence repeat(SSR)markers techniques were applied for the construction of genetic linkage maps in cucumber.To understand the dwarf trait genetic basis in cucumber,the quantitative trait loci(QTL)were identified using F_6 recombinant inbred line populations(RILs)comprising 336 lines from the cross between the two cultivars D0462(the dwarf)and DN129(the vine).In total,six SSR markers and 15 AFLP markers were detected on the five linkage groups covering 152.8 cM with a mean marker interval of 7.28 cM.Only one QTL was found to be linked with plant height and the dwarf trait locus remained in the fourth linkage group.The contributory percentage of the single QTL to plant height was 11.39%.展开更多
基金supported by National Key Research and Development Program of China(Grant Nos.2018YFD1000800 and 2019YFD1000300)National Natural Science Foundation of China(Grant No.31872158)Earmarked Fund for China Agriculture Research System(Grant No.CAS-23).
文摘P450(cytochrome P450)is a supergene family,which is involved in various metabolic pathways in plants.Based on previous study,we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts.However,the reason that why P450 mRNAs were endorsed as signaling,and what specific motif(s)did they harbored is not clear yet.Here,we first identified 221 CsaP450 genes in cucumber genomewide level.Combining with graft-transmissiblemRNAs datasets in cucumber,we elucidated 15mobile-CsaP450-coding genes,of which 5 and 10 belonged to A-type and non-A type respectively.Compared with Arabidopsis and pumpkin(Cucubit moschata)graft-induced-transmissible P450 mRNAs,a phylogenetic treewas constructed and divided into eight clans by usingmultiple-sequence alignment.Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways.The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes.The integrated analysis of mobility direction and mRNA abundance of 15 mobilemRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them.The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.
文摘In the entire world, about 200 million populations are exposed to arsenic poisoning in groundwater. Arsenic is a very poisonous metalloid and has three allotropic forms. The toxic inorganic arsenic is converted and utilized metabolically into organic form. It is primarily eliminated out from the human body through metabolic wastes like urine and is also deposited in the hair, nails and skin of the affected ones. Arsenic is known to be a carcinogen as Carcinogen category 1 in the forms of arsenic trioxide, arsenic pentoxide, arsenous acids, arsenic acid and their salts. The arsenic also affects the epidermal system, the nervous system and the vascular system of humans. In the present investigation for toxicological evaluation of arsenic Liver Function Tests & Kidney Function Tests have been considered. It is presumed that medicinal plants have significance in the present day in view of the lethal diseases like AIDS, cancer, hepatitis, nephritis sterility too for which no effective drugs are found till today in modern system of medicine. Medicinal plant based drugs generally have no side effects or the least side effect. Thus to evaluate an ameliorating effect, if any, to mitigate the arsenic toxicity in mice, for which Cucumis sativus (cucumbers) has been taken as curative measure. Sodium arsenite at the dose of 3 mg/kg body weight was administered for 4 weeks followed by the administration of Cucumis sativus (cucumbers) for 4 and 6 weeks at dose of 500 mg/kg body weight. Their biochemical levels like liver and kidney function tests were assayed and were found with elevated levels. But, after administration of aqueous extract of Cucumis sativus (cucumbers), there was significant amelioration in the biochemical levels. The protective effect of Cucumis sativus (cucumbers) was shown in the form of normalization of enzymatic and non-enzymatic activities represented by normalization of liver and kidney functions.
文摘Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that it also exists in the sieve element-companion cell (SE-CC) complex, being widely distributed in P-protein filaments and sieve element reticulum (SER), in the cytoplasm and mitochondrial membrane of companion cell (CC) and in the branched plasmodesmata between sieve element (SE) and CC as well. The results suggest that this protein could be synthesized in CC and transferred to SE through plasmodesmata. Western blotting showed that spectrin-like protein existed in the protein of phloem exudate of cucumber, and its molecular weight was about 260 kD.
文摘Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplified fragment length polymorphism （AFLP） markers are studied to formulate efficient strategies for breeding cultivars resistant to powdery mildew. The joint analysis of multiple generations and AFLP technique has been applied in this study. The best model is the one with two major genes, additive, dominant, and epistatic effects, plus polygenes with additive, dominant, and epistatic effects （E-l-0 model）. The heritabilities of the major genes varied from 64.26% to 97.82%, and susceptibility was incompletely dominant for the two major genes in the three crosses studied. The additive effects of the two major genes and the dominant effect of the second major gene were high, and the epistatic effect of the additive-dominant between the two major genes was the highest in cross I . In cross II, the absolute value of the additive effect, dominant effect, and potential ratio of the first major gene were far higher than those of the second major gene, and the epistatic effect of the additive-additive was the highest. The genetic parameters of the two major genes in cross III were similar to those in cross II. Correlation and regression analyses showed that marker E25/M63-103 was linked to a susceptible gene controlling powdery mildew resistance. The marker could account for 19.98% of the phenotypic variation. When the marker was tested on a diverse set of 29 cucumber lines, the correlation between phenotype and genotype was not significant, which suggested cultivar specialty of gene expression or different methods of resistance to powdery mildew. The target DNA fragment was 103 bp in length, and only a small part was found to be homologous to DNA in the other species evaluated, which indicated that it was unique to the cucumber genome.
基金financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304)the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015)the Beijing Municipal Education Commission, China (KM200910011001)
文摘Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.
文摘Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes.
基金Project supported by Scientific Grant of TianJin Normal University (1CHS02B)Special Grant of the President of TianJin Normal University
文摘There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on microstructure, most reports are focused on the toxicology rather than promotion. Using cucumis sativus L. (Jin Chun No.5) as experiment material, we try to find out the nutritional effects of low Y3+ concentrations on cucumber seedlings′ leaves. The present paper suggests that the rare earth elements act as micronutrients at low concentrations while they give rise to toxicity at high concentration. Benefits defeat toxicity with concentration ranging from 5 to 25 μmol·L-1. Through careful study, at the Y(NO3)3 concentration of 10 μmol·L-1 the content of chlorophyll as well as the activities of SOD, Cu-Zn SOD and the POD are the highest. It indicates 10 μmol·L-1 is the optimum concentration of yttrium for promoting the cucumber growth.
基金This paper is translated from its Chinese version in Scientia Agricultura Sinica.This research was partially supported by the Transcentury Training Program Foundation for the Talents by the Ministry of Education of China to Dr.Chen Jinfeng(30470120)by the National Natural Science Foundation of China(30671419)+2 种基金the National Hi-Tech R&D Program(2004AA241120)the Tang Foundation Cornell-China Scholar Programthe Pickle Seed Research Foundation of Pickle Packers International.The authors sincerely thank Dr.Zhai Huqu,the President of the Chinese Academy of Agricultural Sciences for his support in this research.
文摘An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1 The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively. Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome configurations were observed in the pollen mother cells （PMCs） of hybrid F1 at metaphase Ⅰ , e.g., univalents, bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains （about 23.3%）. Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix x NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus.
基金supported by the National Key Research and Development Program of China (2018YFD1000800)the National Natural Science Foundation of China (32072561 and 31772290)。
文摘Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of plants.Cucumber is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely unknown.Here,we report the functional characteristics of Cs NIP2;2.It was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter gene.Cs NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix position.Xenopus laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low temperature.Cs NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other tissues.Transient expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the cell.The results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other plants.These findings may be helpful for understanding the functions of NIPs in cucumber plants.
基金Supported by the"Academic Backbone"Project of Northeast Agricultural University(18XG06)Supporting Certificate of Heilongjiang Postdoctoral Scientific Research Developmental Fund(LBH-Q16021)the Natural Science Foundation of Heilongjiang Province,China(LH2019C033)。
文摘Plant height in cucumber is not only an important trait for breeding,but also one of the model traits suitable for the study of developmental biology.Amplified fragment length polymorphism(AFLP)and simple sequence repeat(SSR)markers techniques were applied for the construction of genetic linkage maps in cucumber.To understand the dwarf trait genetic basis in cucumber,the quantitative trait loci(QTL)were identified using F_6 recombinant inbred line populations(RILs)comprising 336 lines from the cross between the two cultivars D0462(the dwarf)and DN129(the vine).In total,six SSR markers and 15 AFLP markers were detected on the five linkage groups covering 152.8 cM with a mean marker interval of 7.28 cM.Only one QTL was found to be linked with plant height and the dwarf trait locus remained in the fourth linkage group.The contributory percentage of the single QTL to plant height was 11.39%.
