期刊文献+
共找到743篇文章
< 1 2 38 >
每页显示 20 50 100
黄瓜和南瓜Bcl-2相关抗凋亡家族全基因组鉴定与表达模式分析
1
作者 胡永波 雷雨田 +6 位作者 杨永森 陈馨 林黄昉 林碧英 刘爽 毕格 申宝营 《生物技术通报》 CAS CSCD 北大核心 2024年第6期219-237,共19页
【目的】分析Bcl-2相关抗凋亡(Bcl-2 associated athanogene,BAG)家族蛋白成员在黄瓜和南瓜中响应非生物胁迫以及在黄瓜/南瓜嫁接愈合过程中响应光照的表达模式,为解析黄瓜/南瓜嫁接苗嫁接愈合机理及黄瓜和南瓜等蔬菜的抗性分子育种提... 【目的】分析Bcl-2相关抗凋亡(Bcl-2 associated athanogene,BAG)家族蛋白成员在黄瓜和南瓜中响应非生物胁迫以及在黄瓜/南瓜嫁接愈合过程中响应光照的表达模式,为解析黄瓜/南瓜嫁接苗嫁接愈合机理及黄瓜和南瓜等蔬菜的抗性分子育种提供有利基因。【方法】基于黄瓜和南瓜基因组信息,利用生物信息学手段,对黄瓜和南瓜中BAG基因家族进行鉴定,并对其理化特性、染色体定位、基因结构、系统发育和共线性进行了分析。基于公共数据库及黄瓜/南瓜嫁接苗在嫁接愈合过程中转录组测序数据,分析BAG基因在黄瓜和南瓜中响应非生物胁迫以及在嫁接愈合过程中响应光照的表达模式。【结果】在黄瓜和南瓜中分别鉴定到12和18个BAG家族基因,均分为2个亚族,基因成员保守性高,I亚家族的BAG主要参与基因调控和逆境响应,而II亚家族的BAG主要参与植物的发育过程。黄瓜和南瓜BAG基因分别与拟南芥、水稻、番茄存在多种线性关系,但CsaV3_1G017210与拟南芥、水稻、番茄和南瓜中的BAG基因均不存在线性关系。不同BAG基因具有组织特异性表达模式。CsaV3_6G000970和CmoCh08G008520(BAG family molecular chaperone regulator 6)在黄瓜和南瓜响应非生物胁迫以及黄瓜/南瓜嫁接苗嫁接愈合过程中均发生上调表达。【结论】BAG家族基因在黄瓜和南瓜对非生物胁迫的响应以及黄瓜/南瓜嫁接苗嫁接愈合过程中对光的响应中具有差异性,协同调控了黄瓜和南瓜的生长发育及嫁接愈合,在黄瓜和南瓜非生物胁迫以及黄瓜/南瓜嫁接苗嫁接愈合过程中发挥着重要作用。 展开更多
关键词 黄瓜 南瓜 BAG基因家族 生物信息学 嫁接愈合
下载PDF
外源NO对NaCl胁迫下黄瓜(Cucumis sativus L.)幼苗生长和谷胱甘肽抗氧化酶系统的影响 被引量:34
2
作者 樊怀福 郭世荣 +2 位作者 段九菊 杜长霞 孙锦 《生态学报》 CAS CSCD 北大核心 2008年第6期2511-2517,共7页
采用营养液水培,研究了外源一氧化氮(NO)对黄瓜(Cucumis sativusL.)幼苗生长和叶片谷胱甘肽抗氧化酶系统的影响。结果表明,正常生长条件下添加NO能促进黄瓜幼苗生长,而添加NO信号传递途径关键酶鸟苷酸环化酶(cGC)抑制剂亚甲基蓝(MB-1)... 采用营养液水培,研究了外源一氧化氮(NO)对黄瓜(Cucumis sativusL.)幼苗生长和叶片谷胱甘肽抗氧化酶系统的影响。结果表明,正常生长条件下添加NO能促进黄瓜幼苗生长,而添加NO信号传递途径关键酶鸟苷酸环化酶(cGC)抑制剂亚甲基蓝(MB-1)显著抑制了黄瓜幼苗的生长;添加NO显著缓解了盐胁迫对黄瓜幼苗生长的抑制,提高了叶片谷胱甘肽还原酶(GR)活性、脱氢抗坏血酸还原酶(DHAR)活性、抗坏血酸过氧化物酶(APX)及还原型谷胱甘肽(GSH)、抗坏血酸(ASA)含量,降低了氧化型谷胱甘肽(GSSG)含量,提高了GSH/GSSG,对单脱氢抗坏血酸还原酶(MDAR)活性无显著影响;NaCl胁迫下添加NO的同时添加MB-1抑制了GR活性的提高,GSH和ASA含量、GSH/GSSG均降低,GSSG含量提高,但对MDAR、APX和DHAR活性无显著影响,表明NaCl胁迫下NO对GR活性、GSH和ASA含量、GSH/GSSG的调节可能是通过cGC介导的,对MDAR无明显的调节作用,对DHAR、APX的调节还存在其它途径。 展开更多
关键词 一氧化氮 NACl胁迫 黄瓜(cucumis SATIVUS l.)幼苗 谷胱甘肽
下载PDF
两个黄瓜(Cucumis sativus L.)叶色突变体的比较分析研究 被引量:7
3
作者 苗晗 顾兴芳 +2 位作者 张圣平 方智远 张振贤 《中国蔬菜》 北大核心 2010年第22期16-20,共5页
通过对黄瓜黄绿叶突变体9110Gt和NCG-042植株表型观测、遗传分析和分子标记验证,证明9110Gt是区别于NCG-042的新叶色突变体。这两个黄绿叶突变体在表型上存在一定的区别:9110Gt在苗期表现叶色黄化,而NCG-042的心叶在整个生育期都表现黄... 通过对黄瓜黄绿叶突变体9110Gt和NCG-042植株表型观测、遗传分析和分子标记验证,证明9110Gt是区别于NCG-042的新叶色突变体。这两个黄绿叶突变体在表型上存在一定的区别:9110Gt在苗期表现叶色黄化,而NCG-042的心叶在整个生育期都表现黄化。遗传分析证明,两个突变体的叶色突变性状分别由两个不同的等位基因控制,且两基因间存在互补作用。分子标记的检测结果也进一步证实了这一结论。 展开更多
关键词 黄瓜(cucumis SATIVUS l.) 黄绿叶突变 遗传分析 复等位基因
下载PDF
外源BR诱导黄瓜(Cucumis sativus L.)幼苗的抗盐性 被引量:38
4
作者 尚庆茂 宋士清 +1 位作者 张志刚 郭世荣 《中国农业科学》 CAS CSCD 北大核心 2006年第9期1872-1877,共6页
【目的】明确外源油菜素甾醇(Brassinosteroid,BR)对黄瓜(CucumissativusL.)幼苗抗盐性的诱导作用。【方法】采用根际注射结合叶面喷施外源BR(0、0.005、0.01、0.05、0.1、0.2mg·L-1)的方法,比较分析了盐胁迫下幼苗植株盐害指数、... 【目的】明确外源油菜素甾醇(Brassinosteroid,BR)对黄瓜(CucumissativusL.)幼苗抗盐性的诱导作用。【方法】采用根际注射结合叶面喷施外源BR(0、0.005、0.01、0.05、0.1、0.2mg·L-1)的方法,比较分析了盐胁迫下幼苗植株盐害指数、抗氧化酶活性、渗透调节物质含量等生理指标。【结果】外源BR能够明显改善盐胁迫下黄瓜幼苗植株的生长发育状况,降低盐害指数,最高幅度达40.2个百分点(P﹤0.01),极显著地提高叶片细胞游离脯氨酸(Pro)和可溶性糖的含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性(P﹤0.01),进而保护细胞膜的稳定性。【结论】外源BR可以有效诱导黄瓜幼苗的抗盐性,并且最佳浓度范围是0.01~0.05mg·L-1。 展开更多
关键词 油菜素内酯 黄瓜 抗盐性 诱导
下载PDF
外源亚精胺对盐胁迫下黄瓜(Cucumis sativus L.)叶绿体活性氧清除系统和结合态多胺含量的影响 被引量:16
5
作者 段九菊 郭世荣 +2 位作者 康云艳 周国贤 刘香娥 《生态学报》 CAS CSCD 北大核心 2009年第2期653-661,共9页
采用营养液水培,研究了外源亚精胺(Spd)对NaCl胁迫下抗盐能力不同的两个黄瓜品种幼苗生长、叶绿体中活性氧清除系统、转谷酰胺酶(TGase)活性、结合态多胺含量及植株光合速率的影响。结果表明,外源Spd能提高NaCl胁迫下叶绿体中TGase活性... 采用营养液水培,研究了外源亚精胺(Spd)对NaCl胁迫下抗盐能力不同的两个黄瓜品种幼苗生长、叶绿体中活性氧清除系统、转谷酰胺酶(TGase)活性、结合态多胺含量及植株光合速率的影响。结果表明,外源Spd能提高NaCl胁迫下叶绿体中TGase活性、叶绿体结合态腐胺(Put)、Spd、精胺(Spm)及总多胺含量;提高超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性,提高抗坏血酸(AsA)、类胡萝卜素(Car)、还原型谷胱甘肽(GSH)含量及还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)比值,降低脱氢抗坏血酸/抗坏血酸(DAsA/AsA)比值;同时显著降低叶绿体过氧化氢(H2O2)和丙二醛(MDA)含量,提高植株净光合速率,缓解NaCl胁迫对幼苗生长的抑制。表明Spd对黄瓜盐害的缓解作用之一可能是通过提高叶绿体结合态多胺含量和叶绿体活性氧清除能力,从而缓解盐胁迫对叶绿体膜的伤害。 展开更多
关键词 黄瓜 盐胁迫 叶绿体 活性氧清除系统 多胺
下载PDF
侧面补光对温室黄瓜(Cucumis sativus L.cv.Shenchun)果实生长和品质的影响 被引量:10
6
作者 谢景 刘厚诚 +2 位作者 宋世威 孙光闻 陈日远 《沈阳农业大学学报》 CAS CSCD 北大核心 2013年第5期616-621,共6页
为研究侧面补光对温室黄瓜果实生长和品质的影响,以黄瓜品种深春为材料,采用不同光质(8红1蓝、6红2绿1蓝、6红3蓝)LED灯开花结果期侧面补光,光源光通量密度为15μmol·m-2·s-1。每天补光8.0h(06:00-11:00;16:30-19:30),测定分... 为研究侧面补光对温室黄瓜果实生长和品质的影响,以黄瓜品种深春为材料,采用不同光质(8红1蓝、6红2绿1蓝、6红3蓝)LED灯开花结果期侧面补光,光源光通量密度为15μmol·m-2·s-1。每天补光8.0h(06:00-11:00;16:30-19:30),测定分析了侧面补光下黄瓜果实生长和品质等的变化。结果表明:3个不同补光处理的黄瓜果实瓜长、径粗及瓜重均高于对照处理,3个不同补光处理均明显提高黄瓜果实维生素C含量和氨基酸总含量,其中,维生素C含量以6红3蓝(6R3B)补光处理最高,8种人体必需氨基酸以及其他氨基酸含量和比例均以8红1蓝(8R1B)补光处理最高;而可溶性蛋白、可溶性糖、蔗糖、果糖、淀粉和还原糖含量,仅8红1蓝(8R1B)补光处理比自然光有显著提高。因此,开花结果期不同光质LED灯侧面补光能促进黄瓜果实生长和营养品质的增加,红光比例较高的8红1蓝(8R1B)补光处理效果最好。 展开更多
关键词 黄瓜 lED补光 侧面补光 果实生长 果实品质
下载PDF
黄瓜(Cucumis sativus L.)种子含油量性状的QTL定位与分析 被引量:5
7
作者 李坤 司龙亭 +3 位作者 张克岩 姜晶 田友 李丹丹 《分子植物育种》 CAS CSCD 2011年第2期198-203,共6页
应用SRAP和SSR技术,对黄瓜种子高含油量品系Ma7与低含油量品系M6杂交组合的F2群体进行检测,获得102个分子标记,构建了7个连锁群组成的分子标记遗传图谱;图谱总长764cM,标记间平均长度7.49cM。应用Windows QTL Cartographer2.5对种子含... 应用SRAP和SSR技术,对黄瓜种子高含油量品系Ma7与低含油量品系M6杂交组合的F2群体进行检测,获得102个分子标记,构建了7个连锁群组成的分子标记遗传图谱;图谱总长764cM,标记间平均长度7.49cM。应用Windows QTL Cartographer2.5对种子含油量性状进行QTL扫描,在2009年秋季获得4个QTL位点,分别位于LG1、LG2和LG5连锁群,联合贡献率为32.7%。在2010年春季季获得5个QTL位点,分别位于LG1、LG2、LG5和LG6连锁群,联合贡献率为35.4%。通过对比春秋两季不同环境下的QTL位点发现,SOC_SP02和SOC_FA01分别位于LG1的74.7cM和72.2cM处,两位点相距2.5cM。此外,SOC_SP03和SOC_FA03两个微效QTL也存在部分的重叠,重叠区域为1.67cM,重叠的区间位于分子标记ME7OD17a和ME3EM8之间。另外,还估算了单个QTL的加性效应和显性效应,9个QTL的遗传效应均以加性效应为主。结果表明,对于种子油含量性状的等位基因增效作用既来自母本,又源自父本。 展开更多
关键词 黄瓜 种子含油量 QTl
下载PDF
来自甜瓜属(Cucumis melo L.)6个变种部分品种货架期差异比较 被引量:5
8
作者 刘莉 柿原文香 加藤正弘 《园艺学报》 CAS CSCD 北大核心 2004年第6期817-819,共3页
对甜瓜属6个变种的13个材料的货架期及相关因素进行了测定比较。结果表明,来自var.saccharinus和inodorus的5个品系表现出乙烯释放速率和鲜样质量损失率很低,货架期显著优于其它变种的材料。var.reticulatus虽然乙烯释放量较低,但由于... 对甜瓜属6个变种的13个材料的货架期及相关因素进行了测定比较。结果表明,来自var.saccharinus和inodorus的5个品系表现出乙烯释放速率和鲜样质量损失率很低,货架期显著优于其它变种的材料。var.reticulatus虽然乙烯释放量较低,但由于果肉软化,其货架期短于var.inodorus。var.cantalupensis,makuwa和acidulus货架期短。相关性分析显示货架期还与果实发育期及果实质量存在高度相关,而果实表面的网纹结构与乙烯释放速率、鲜样质量损失速率及货架期并无显著的相关性。 展开更多
关键词 甜瓜属 变种 货架期 乙烯
下载PDF
黄瓜(Cucumis sativus L.)组织培养与诱导四倍体再生植株 被引量:24
9
作者 张承妹 陆家安 《上海农业学报》 CSCD 1995年第3期31-36,共6页
应用杨行黄瓜、长春密刺、农大秋光和杂种群丰、津研四号、津杂四号等黄瓜的子叶、真叶、茎段为外植体,离体培养诱导再生植株。比较了细胞分裂素(KT)与不同浓度的2,4-D和NAA配比对诱导黄瓜愈伤组织的影响,及其所产生的愈... 应用杨行黄瓜、长春密刺、农大秋光和杂种群丰、津研四号、津杂四号等黄瓜的子叶、真叶、茎段为外植体,离体培养诱导再生植株。比较了细胞分裂素(KT)与不同浓度的2,4-D和NAA配比对诱导黄瓜愈伤组织的影响,及其所产生的愈伤组织分化培养的成苗率。所得结果表明:黄瓜在组织培养再生植株时,对生长激素极为敏感,只需极低浓度的2,4-D或NAA即能诱导大量愈伤组织。激素浓度增高,出愈率提高,愈伤组织增殖加快,但分化能力下降。NAA诱导成的愈伤组织比2,4-D诱导的易于分化。在诱导愈伤组织及胚性细胞发生时加入500mg·L-1的秋水仙素经振荡液体培养48,72,96h再进行分化培养,出苗率分别为25.6%、18.5%、0.9%,对照为46.8%。再生植株中3.5%~4.5%为四倍体,秋水仙素在分化培养中有抑制作用,处理96h的则很少分化再生植株。 展开更多
关键词 黄瓜 组织培养 秋水仙素 四倍体 再生植株
下载PDF
河套蜜瓜(Cucumis melo L cv Hetau)子叶的组织培养和再生植株研究 被引量:3
10
作者 张治中 李天然 +1 位作者 邓香兰 姜金安 《内蒙古大学学报(自然科学版)》 CAS CSCD 1998年第2期235-239,共5页
切取在MS培养基中生长5d的河套蜜瓜子叶,在MS+NAA1+BA0.1的培养基中予培养3d后转入芽诱导培养基(MS+ZT6)诱导生芽,待芽长2cm左右转入MS+IBA0.5诱导生根,10d后再转入沙质土壤的营养缶本中... 切取在MS培养基中生长5d的河套蜜瓜子叶,在MS+NAA1+BA0.1的培养基中予培养3d后转入芽诱导培养基(MS+ZT6)诱导生芽,待芽长2cm左右转入MS+IBA0.5诱导生根,10d后再转入沙质土壤的营养缶本中成苗,整个成苗过程约需60~70d,再生植株诱导率可达55%。 展开更多
关键词 河套蜜瓜 子叶 再生植株 组织培养
下载PDF
黄瓜类钙调蛋白CML8与性型分化主效基因编码蛋白的互作分析
11
作者 许俊强 张新梅 +4 位作者 吕霞 苏甜 张国平 张应华 许彬 《西南农业学报》 CSCD 北大核心 2023年第5期904-912,共9页
【目的】为探索类钙调蛋白CMLs是否参与黄瓜性型分化过程,以便进一步完善性型分化调控网络提供参考。【方法】通过克隆黄瓜CML8、ACS1G、ACS2及ACS11基因,qPCR检测CML8基因在不同花型中的表达量,酵母双杂交检测CML8与黄瓜性型分化主效... 【目的】为探索类钙调蛋白CMLs是否参与黄瓜性型分化过程,以便进一步完善性型分化调控网络提供参考。【方法】通过克隆黄瓜CML8、ACS1G、ACS2及ACS11基因,qPCR检测CML8基因在不同花型中的表达量,酵母双杂交检测CML8与黄瓜性型分化主效基因编码蛋白间的互作情况。【结果】克隆得到黄瓜CML8基因,完整ORF 441 bp,编码146 aa,不含信号肽;不含跨膜域,为胞外蛋白,含4个具有Ca^(2+)结合能力的EF-hand结构域,不含内含子,亚细胞定位在细胞膜和细胞质;克隆得到黄瓜性型分化关键基因F(ACS1G)、M(ACS2)及A(ACS11),序列比对表明三者的基因序列及编码蛋白序列与黄瓜数据库中的一致,且在雌花、雄花和两性花3种不同花型中的表达量差异显著,在雄花中差异极显著;酵母双杂交试验表明,CML8均可与ACS1G和ACS2发生互作,而不能与ACS11发生互作。【结论】成功克隆得到黄瓜CML8基因,且CML8可与ACS1G和ACS2发生互作,可能参与黄瓜性型分化过程,本研究首次探索类钙调蛋白参与植物性型分化过程,期望为钙信号系统参与植物性型分化提供参考。 展开更多
关键词 黄瓜 类钙调蛋白(CMl8) 性型分化 主效基因 蛋白互作
下载PDF
哈密瓜(Cucumis melo L.)致腐真菌的鉴定 被引量:2
12
作者 乔言平 吴朝霞 +4 位作者 丁真真 王宇滨 马越 赵晓燕 张超 《食品工业科技》 CAS CSCD 北大核心 2018年第23期123-127,133,共6页
研究从哈密瓜表面分离出引起其腐败的菌株A和菌株B,并采用显微镜形态学观察、表型特征和分子生物学三种方法对其进行鉴定。结果发现菌株A可利用碳源包括杏苷、糊精、D-半乳糖、D-葡萄糖酸、麦芽糖、肝糖、L-鼠李糖、L-山梨糖、L-乳酸等7... 研究从哈密瓜表面分离出引起其腐败的菌株A和菌株B,并采用显微镜形态学观察、表型特征和分子生物学三种方法对其进行鉴定。结果发现菌株A可利用碳源包括杏苷、糊精、D-半乳糖、D-葡萄糖酸、麦芽糖、肝糖、L-鼠李糖、L-山梨糖、L-乳酸等72种碳源,其ITS rDNA序列与尖孢镰刀菌甜瓜转化型(Fusarium oxysporum f.sp.melonis)的同源性为99%,分支长度为0.00。菌株B可利用碳源包括L-阿拉伯糖、D-果糖、丙三醇、蔗糖和木糖醇等54种碳源,其ITS rDNA序列与链格孢菌(Alternaria alternata)的同源性为100%,分支长度为0.00。因此,推断菌株A为尖孢镰刀菌甜瓜转化型,菌株B为链格孢菌。 展开更多
关键词 哈密瓜 致腐菌 ITS(Internal transcribed spacer)测序 BIOlOG PMs表型芯片系统
下载PDF
Current status of genetic transformation technology developed in cucumber(Cucumis sativus L.) 被引量:8
13
作者 WANG Shun-li Seong Sub Ku +3 位作者 YE Xing-guo HE Cong-fen Suk Yoon Kwon Pil Son Choi 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第3期469-482,共14页
Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major... Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings. 展开更多
关键词 cucumis sativus l. plant regeneration genetic transformation positive selection system
下载PDF
Inheritance of Powdery Mildew Resistance in Cucumber (Cucumis sativus L.) and Development of an AFLP Marker for Resistance Detection 被引量:8
14
作者 ZHANG Su-qin GU Xing-fang +1 位作者 ZHANG Sheng-ping ZOU Zhi-rong 《Agricultural Sciences in China》 CAS CSCD 2007年第11期1336-1342,共7页
Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplifi... Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplified fragment length polymorphism (AFLP) markers are studied to formulate efficient strategies for breeding cultivars resistant to powdery mildew. The joint analysis of multiple generations and AFLP technique has been applied in this study. The best model is the one with two major genes, additive, dominant, and epistatic effects, plus polygenes with additive, dominant, and epistatic effects (E-l-0 model). The heritabilities of the major genes varied from 64.26% to 97.82%, and susceptibility was incompletely dominant for the two major genes in the three crosses studied. The additive effects of the two major genes and the dominant effect of the second major gene were high, and the epistatic effect of the additive-dominant between the two major genes was the highest in cross I . In cross II, the absolute value of the additive effect, dominant effect, and potential ratio of the first major gene were far higher than those of the second major gene, and the epistatic effect of the additive-additive was the highest. The genetic parameters of the two major genes in cross III were similar to those in cross II. Correlation and regression analyses showed that marker E25/M63-103 was linked to a susceptible gene controlling powdery mildew resistance. The marker could account for 19.98% of the phenotypic variation. When the marker was tested on a diverse set of 29 cucumber lines, the correlation between phenotype and genotype was not significant, which suggested cultivar specialty of gene expression or different methods of resistance to powdery mildew. The target DNA fragment was 103 bp in length, and only a small part was found to be homologous to DNA in the other species evaluated, which indicated that it was unique to the cucumber genome. 展开更多
关键词 cucumber cucumis sativus l.) powdery mildew resistance INHERITANCE amplified fragment lengthpolymorphism (AFlP) molecular marker
下载PDF
Cloning and Sequence Analysis of Lipoxygenase Gene cDNA from Cucumber Fruit (Cucumis sativus L.) 被引量:1
15
作者 Z.K. Wang  Z.W. Qin  X.Y. Zhou  D.Y. Song 《分子植物育种》 CAS CSCD 2007年第2期188-189,共2页
Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes.
关键词 脂加氧酶 基因克隆 黄瓜 种植
下载PDF
Genome-wide association study reveals candidate genes for gummy stem blight resistance in cucumber 被引量:2
16
作者 Jianan Han Shaoyun Dong +5 位作者 Yanxia Shi Zhuonan Dai Han Miao Baoju Li Xingfang Gu Shengping Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第2期261-272,共12页
Gummy stem blight(GSB),caused by Didymella bryoniae,is a serious fungal disease that leads to decline in cucumber yield and quality.The molecular mechanism of GSB resistance in cucumber remains unclear.Here,we investi... Gummy stem blight(GSB),caused by Didymella bryoniae,is a serious fungal disease that leads to decline in cucumber yield and quality.The molecular mechanism of GSB resistance in cucumber remains unclear.Here,we investigated the GSB resistance of cucumber core germplasms from four geographic groups at the seedling and adult stages.A total of 9 SNPs related to GSB resistance at the seedling stage and 26 SNPs at the adult stage were identified,of which some are co-localized to previously mapped Quantitative trait loci(QTLs)for GSB resistance(gsb3.2/gsb3.3,gsb5.1,and gsb-s6.2).Based on haplotype analysis and expression levels after inoculation,four candidate genes were identified within the region identified by both Genome-wide association study(GWAS)and previous identified QTL mapping,including Csa3G129470 for gsb3.2/gsb3.3,Csa5G606820 and Csa5G606850 for gsb5.1,and Csa6G079730 for gsb-s6.2.The novel GSB resistant accessions,significant SNPs,and candidate genes facilitate the breeding of GSB resistant cucumber cultivars and provide a novel idea for understanding GSB resistance mechanism in cucumber. 展开更多
关键词 Cucumber(cucumis sativus l.) GSB GWAS Quantitative trait loci QTl Candidate genes
下载PDF
Effect of Low Concentration of Yttrium on Physiological Characteristics of Cucumber (Cucumis Sativus L.)
17
作者 王烁 陈丹 +3 位作者 秦兆江 董振宇 鞠明超 解冰凝 《Journal of Rare Earths》 SCIE EI CAS CSCD 2007年第S2期236-241,共6页
There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on micro... There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on microstructure, most reports are focused on the toxicology rather than promotion. Using cucumis sativus L. (Jin Chun No.5) as experiment material, we try to find out the nutritional effects of low Y3+ concentrations on cucumber seedlings′ leaves. The present paper suggests that the rare earth elements act as micronutrients at low concentrations while they give rise to toxicity at high concentration. Benefits defeat toxicity with concentration ranging from 5 to 25 μmol·L-1. Through careful study, at the Y(NO3)3 concentration of 10 μmol·L-1 the content of chlorophyll as well as the activities of SOD, Cu-Zn SOD and the POD are the highest. It indicates 10 μmol·L-1 is the optimum concentration of yttrium for promoting the cucumber growth. 展开更多
关键词 rare earth elements YTTRIUM physiological characteristics promoting effects cucumis sativus l.
下载PDF
Cytological Analyses on Development of Male and Female Gametophytes in an Interspecific Hybrid F_1 from Cucumis hystrix Chakr. × Cucumis sativus L.
18
作者 LUO Xiang-dong DAI Liang-fang +1 位作者 QIAN Chun-tao CHEN Jin-feng 《Agricultural Sciences in China》 CAS CSCD 2006年第11期820-827,共8页
An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. (NC4406) was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for furth... An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. (NC4406) was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1 The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively. Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome configurations were observed in the pollen mother cells (PMCs) of hybrid F1 at metaphase Ⅰ , e.g., univalents, bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains (about 23.3%). Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix x NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus. 展开更多
关键词 cucumis hystrix Chakr. C. sativus l. interspecific hybrid male gametophytes female gametophytes
下载PDF
Growth promotion of interspecific hybrid embryos between Cucumis anguria and Melon(C. melo) by fruit heating
19
作者 Yuichi Matsumoto Mako Tsunamoto 《Horticultural Plant Journal》 SCIE CSCD 2022年第2期215-220,共6页
Wild species of the genus Cucumis, such as C. anguria, are resistant to various melon(C. melo) diseases such as Fusarium wilt race 1.2 and are anticipated as genetic resources of melon. However, in the interspecific c... Wild species of the genus Cucumis, such as C. anguria, are resistant to various melon(C. melo) diseases such as Fusarium wilt race 1.2 and are anticipated as genetic resources of melon. However, in the interspecific crosses between C. anguria and melon, the abortion of the embryos was observed in the early growth stage and could not be regenerated even when cultured. Therefore, for embryo rescue, it is necessary to promote embryo growth during abortion occurrence. This study investigated the effects of fruit heating on embryo growth in interspecific hybrids of C. anguria and melon. In the heating device, the minimum night temperature around the fruit was maintained above 21.5 °C. The ovule grain area of C. anguria × MR-1 with fruit heating was significantly enlarged compared to that without fruit-heating. It was also significantly enlarged compared with the ovule grain area of C. anguria self-pollination. Although, in C. anguria × MR-1 without fruit-heating, no embryo was observed until 10 days after pollination(DAP), and pre-embryos were observed at 13 DAP. Conversely, in C. anguria × MR-1 with fruit-heating, pre-embryos were even observed at 10 DAP. These results suggest that the possibility of embryo or ovule culture regeneration has increased due to embryo growth promotion. 展开更多
关键词 cucumis anguria l. Interspecific cross MElON Night temperature Ovule area
下载PDF
Nodulin 26-like intrinsic protein Cs NIP2;2 is a silicon influx transporter in Cucumis sativus L.
20
作者 DUAN Yao-ke SU Yan +2 位作者 HAN Rong SUN Hao GONG Hai-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第3期685-696,共12页
Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma m... Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of plants.Cucumber is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely unknown.Here,we report the functional characteristics of Cs NIP2;2.It was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter gene.Cs NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix position.Xenopus laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low temperature.Cs NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other tissues.Transient expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the cell.The results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other plants.These findings may be helpful for understanding the functions of NIPs in cucumber plants. 展开更多
关键词 cucumber(cucumis sativus l.) nodulin 26-like intrinsic membrane protein(NIP) silicon influx transporter aromatic/arginine selectivity filter
下载PDF
上一页 1 2 38 下一页 到第
使用帮助 返回顶部