Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to ...Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.展开更多
Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method...Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.展开更多
The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy du...The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color （under light microscopy） and gold particles （under transmission electron microscopy） for detecting caspase 3-like protease was mainly displayed in inner phloem, external phloem and xylem in the region close to procambium. From the results it was considered that caspase 3-like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3-like protease mainly participated in making cytoplasmic streaming cease and in degrading P-protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.展开更多
Objective:To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne(tropical pumpkin)(C.moschata)against human pathogenic bacteria.Methods:In the present study,dichloromethane(DCM),met...Objective:To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne(tropical pumpkin)(C.moschata)against human pathogenic bacteria.Methods:In the present study,dichloromethane(DCM),methanol(MEOH)and aqueous extracts of C.moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e.Bacillus cereus,Burkholderia cepacia,Escherichia coli,Enterococcus faecalis,Staphyloccocus aureus,Pseudomonas aerugenosa,Vibrio alginolyticus,Vibrio parahaemolyticus using Kirby-Bauer disk diffusion susceptibility and broth micro-dilution methods.Results:DCM extract of pumpkin peel exhibited the maximum zone of inhibition against Staphyloccocus aureus(21 mm)whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against Escherichia coli(8 mm).MEOH extract gave maximum zone of inhibition against Pseudomonas aerugenosa(19 mm).Broth micro-dilution method showed minimum inhibitory concentration for the DCM extract against Burkholderia cepacia at 6.25 mg/mL.The minimum bactericidal concentrations were also determined to know the nature of all extracts.DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the Vibrio alginolyticus.The minimum bactericidal concentrations values exhibited bactericidal nature ranging from 3.12 mg/mL to 100.00 mg/mL.The screening of antimicrobial properties of different extracts of C.moschata peel revealed that the DCM extract possessed good antimicrobial efficacy compared to MEOH and aqueous extracts.Conclusions:Peel of C.moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics.展开更多
选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南...选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南瓜的维生素C含量最高,红板栗南瓜次之;德丰银栗的可溶性糖含量最高。可见,德丰银栗和红板栗南瓜在物理性状和营养成分上表现的综合品质较优,是莱阳市值得推广和发展的优良品种。展开更多
基金supported by the National Natural Science Foundation of China (No. 39970872, 30772587)the Natural Science Foundation of Fujian Province (C97052,C0510012)+1 种基金Special Fund of Fujian Development and Reform Commission (2006-34)supported by the National Science Foundation of USA
文摘Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest"Technology Research and Demonstration of Pumpkin Industry"(201303112)"Twelfth Five-Year"National Science and Technology Programin Rural Areas"Heterosis Utilization and New Variety Breeding of Pumpkin"(2012BAD02B03-17)
文摘Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.
基金Supported by the National Natural Science Foundation of China (30470863).
文摘The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color （under light microscopy） and gold particles （under transmission electron microscopy） for detecting caspase 3-like protease was mainly displayed in inner phloem, external phloem and xylem in the region close to procambium. From the results it was considered that caspase 3-like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3-like protease mainly participated in making cytoplasmic streaming cease and in degrading P-protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.
基金Supported by Functional Food and Nutraceutical Research Cluster Unit(Grant No.RU 06),Research Management Center,IIUM,Malaysia.
文摘Objective:To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne(tropical pumpkin)(C.moschata)against human pathogenic bacteria.Methods:In the present study,dichloromethane(DCM),methanol(MEOH)and aqueous extracts of C.moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e.Bacillus cereus,Burkholderia cepacia,Escherichia coli,Enterococcus faecalis,Staphyloccocus aureus,Pseudomonas aerugenosa,Vibrio alginolyticus,Vibrio parahaemolyticus using Kirby-Bauer disk diffusion susceptibility and broth micro-dilution methods.Results:DCM extract of pumpkin peel exhibited the maximum zone of inhibition against Staphyloccocus aureus(21 mm)whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against Escherichia coli(8 mm).MEOH extract gave maximum zone of inhibition against Pseudomonas aerugenosa(19 mm).Broth micro-dilution method showed minimum inhibitory concentration for the DCM extract against Burkholderia cepacia at 6.25 mg/mL.The minimum bactericidal concentrations were also determined to know the nature of all extracts.DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the Vibrio alginolyticus.The minimum bactericidal concentrations values exhibited bactericidal nature ranging from 3.12 mg/mL to 100.00 mg/mL.The screening of antimicrobial properties of different extracts of C.moschata peel revealed that the DCM extract possessed good antimicrobial efficacy compared to MEOH and aqueous extracts.Conclusions:Peel of C.moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics.
文摘选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南瓜的维生素C含量最高,红板栗南瓜次之;德丰银栗的可溶性糖含量最高。可见,德丰银栗和红板栗南瓜在物理性状和营养成分上表现的综合品质较优,是莱阳市值得推广和发展的优良品种。
