Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-...Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B pathway.CYLD is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly expressed.Emerging studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like behaviors.However,the precise role of CYLD in glutamatergic neurons is largely unknown.Here,we first proposed involvement of CYLD in cued fear expression.We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons.Our results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male mice.Further,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male mice.Altogether,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial activation.This may contribute,at least in part,to cued fear expression.展开更多
A search strategy based on the maximal information gain principle is presented for the cued search of phased array radars. First, the method for the determination of the cued search region, arrangement of beam positio...A search strategy based on the maximal information gain principle is presented for the cued search of phased array radars. First, the method for the determination of the cued search region, arrangement of beam positions, and the calculation of the prior probability distribution of each beam position is discussed. And then, two search algorithms based on information gain are proposed using Shannon entropy and Kullback-Leibler entropy, respectively. With the proposed strategy, the information gain of each beam position is predicted before the radar detection, and the observation is made in the beam position with the maximal information gain. Compared with the conventional method of sequential search and confirm search, simulation results show that the proposed search strategy can distinctly improve the search performance and save radar time resources with the same given detection probability.展开更多
A cued search algorithm with uncertain detection performance is proposed for phased array radars. Firstly, a target search model based on the information gain criterion is presented with known detection performance, a...A cued search algorithm with uncertain detection performance is proposed for phased array radars. Firstly, a target search model based on the information gain criterion is presented with known detection performance, and the statistical characteristic of the detection probability is calculated by using the fluctuant model of the target radar cross section （RCS）. Secondly, when the detection probability is completely unknown, its probability density function is modeled with a beta distribution, and its posterior probability distribution with the radar observation is derived based on the Bayesian theory. Finally simulation results show that the cued search algorithm with a known RCS fluctuant model can achieve the best performance, and the algorithm with the detection probability modeled as a beta distribution is better than that with a random selected detection probability because the model parameters can be updated by the radar observation to approach to the real value of the detection probability.展开更多
No study so far has specifically addressed the influence of individual differences in trait-anxiety on aversive classical conditioning as indexed by the startle reflex response. We compared the startle reflex response...No study so far has specifically addressed the influence of individual differences in trait-anxiety on aversive classical conditioning as indexed by the startle reflex response. We compared the startle reflex responses between participants classified as high (n = 25) and low (n = 26) in trait-anxiety while undergoing a single-cue aversive classical conditioning procedure. High trait-anxiety group showed a greater startle response to the CS relative to the ITI at the post-acquisition compared with the pre-acquisition phase. Low trait-anxiety group did not show such a clear pattern of conditioning, and results from this group seem to be concealed by differences in the startle responses to the CS and the ITI during the pre-acquisition phase. However, a post-hoc analysis in which such differences at pre-conditioning were removed showed no conditioning effects in low trait-anxiety participants. Taking together, these results suggest differences between high and low trait-anxiety groups in the acquisition of the CS-US association. However, further research should clarify the unexpected pattern of responses shown by low trait-anxiety group.展开更多
The ventral part of the anteromedial thalamic nucleus(AMv)is in a position to convey information to the cortico-hippocampal-amygdalar circuit involved in the processing of fear memory.Corticotropin-releasing-factor(CR...The ventral part of the anteromedial thalamic nucleus(AMv)is in a position to convey information to the cortico-hippocampal-amygdalar circuit involved in the processing of fear memory.Corticotropin-releasing-factor(CRF)neurons are closely associated with the regulation of stress and fear.However,few studies have focused on the role of thalamic CRF neurons in fear memory.In the present study,using a conditioned fear paradigm in CRF transgenic mice,we found that the c-Fos protein in the AMv CRF neurons was significantly increased after cued fear expression.Chemogenetic activation of AMv CRF neurons enhanced cued fear expression,whereas inhibition had the opposite effect on the cued fear response.Moreover,chemogenetic manipulation of AMv CRF neurons did not affect fear acquisition or contextual fear expression.In addition,anterograde tracing of projections revealed that AMv CRF neurons project to wide areas of the cerebral cortex and the limbic system.These results uncover a critical role of AMv CRF neurons in the regulation of conditioned fear memory.展开更多
基金supported by the National Natural Science Foundation of China,Nos.32371065(to CL)and 32170950(to LY)the Natural Science Foundation of the Guangdong Province,No.2023A1515010899(to CL)the Science and Technology Projects in Guangzhou,Nos.2023A4J0578 and 2024A03J0180(to CW)。
文摘Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B pathway.CYLD is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly expressed.Emerging studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like behaviors.However,the precise role of CYLD in glutamatergic neurons is largely unknown.Here,we first proposed involvement of CYLD in cued fear expression.We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons.Our results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male mice.Further,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male mice.Altogether,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial activation.This may contribute,at least in part,to cued fear expression.
基金the High Technology Research and Development Programme of China (2003AA134030)
文摘A search strategy based on the maximal information gain principle is presented for the cued search of phased array radars. First, the method for the determination of the cued search region, arrangement of beam positions, and the calculation of the prior probability distribution of each beam position is discussed. And then, two search algorithms based on information gain are proposed using Shannon entropy and Kullback-Leibler entropy, respectively. With the proposed strategy, the information gain of each beam position is predicted before the radar detection, and the observation is made in the beam position with the maximal information gain. Compared with the conventional method of sequential search and confirm search, simulation results show that the proposed search strategy can distinctly improve the search performance and save radar time resources with the same given detection probability.
基金supported by the National Natural Science Foundation of China (61372165)the Postdoctoral Science Foundation of China (201150M15462012T50874)
文摘A cued search algorithm with uncertain detection performance is proposed for phased array radars. Firstly, a target search model based on the information gain criterion is presented with known detection performance, and the statistical characteristic of the detection probability is calculated by using the fluctuant model of the target radar cross section （RCS）. Secondly, when the detection probability is completely unknown, its probability density function is modeled with a beta distribution, and its posterior probability distribution with the radar observation is derived based on the Bayesian theory. Finally simulation results show that the cued search algorithm with a known RCS fluctuant model can achieve the best performance, and the algorithm with the detection probability modeled as a beta distribution is better than that with a random selected detection probability because the model parameters can be updated by the radar observation to approach to the real value of the detection probability.
文摘No study so far has specifically addressed the influence of individual differences in trait-anxiety on aversive classical conditioning as indexed by the startle reflex response. We compared the startle reflex responses between participants classified as high (n = 25) and low (n = 26) in trait-anxiety while undergoing a single-cue aversive classical conditioning procedure. High trait-anxiety group showed a greater startle response to the CS relative to the ITI at the post-acquisition compared with the pre-acquisition phase. Low trait-anxiety group did not show such a clear pattern of conditioning, and results from this group seem to be concealed by differences in the startle responses to the CS and the ITI during the pre-acquisition phase. However, a post-hoc analysis in which such differences at pre-conditioning were removed showed no conditioning effects in low trait-anxiety participants. Taking together, these results suggest differences between high and low trait-anxiety groups in the acquisition of the CS-US association. However, further research should clarify the unexpected pattern of responses shown by low trait-anxiety group.
基金This work was supported by the National Natural Science Foundation of China(32000716 and 91732304)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB02030001).
文摘The ventral part of the anteromedial thalamic nucleus(AMv)is in a position to convey information to the cortico-hippocampal-amygdalar circuit involved in the processing of fear memory.Corticotropin-releasing-factor(CRF)neurons are closely associated with the regulation of stress and fear.However,few studies have focused on the role of thalamic CRF neurons in fear memory.In the present study,using a conditioned fear paradigm in CRF transgenic mice,we found that the c-Fos protein in the AMv CRF neurons was significantly increased after cued fear expression.Chemogenetic activation of AMv CRF neurons enhanced cued fear expression,whereas inhibition had the opposite effect on the cued fear response.Moreover,chemogenetic manipulation of AMv CRF neurons did not affect fear acquisition or contextual fear expression.In addition,anterograde tracing of projections revealed that AMv CRF neurons project to wide areas of the cerebral cortex and the limbic system.These results uncover a critical role of AMv CRF neurons in the regulation of conditioned fear memory.
