Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase an...Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.Results Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. Conclusion Human oral keratinocytes have been successfully grown in serial culture.展开更多
Fifteen bioluminescent fungi recently collected from Peninsular Malaysia are analysed herein.The phylogeny of the Malaysian bioluminescent fungi and closely related taxa were evaluated with molecular data from the nuc...Fifteen bioluminescent fungi recently collected from Peninsular Malaysia are analysed herein.The phylogeny of the Malaysian bioluminescent fungi and closely related taxa were evaluated with molecular data from the nuclear ribosomal large subunit(LSU),RNA polymerase 2 second largest subunit(RPB2)and internal transcribed spacers(ITS)gene regions.DNA sequences data support the circumscription of species based on the morphological species concept,but was unable to fully support the current sectional delimitation of genus Mycena.Of the 15 taxa analysed here,four are novel species described herein,viz.Mycena gombakensis,M.nocticaelum,M.coralliformis and Panellus luxfilamentus;while four taxa of Mycena sect.Calodontes were recently described as new.One species(M.noctilucens)represents a new distribution record to Peninsular Malaysia,and six species(Neonothopanus nambi,Filoboletus manipularis,P.luminescens,Roridomyces pruinosoviscidus,M.chlorophos and M.illuminans)have been previously reported.Roridomyces pruinosoviscidus is accepted as a new combination based on morphological and phylogenetic data.Culture morphology data indicate its potential for taxon delimitation as axenic cultures of each species produced unique and distinguishable characteristics.Comprehensive descriptions,illustrations and photographs are provided on basidiome and culture morphology.A key to aid in species identification,comparisons with allied species and data on basidiome and mycelium luminescence are also provided.This study gives the first report on mycelial luminescence for M.noctilucens,P.luminescens,R.pruinosoviscidus as well as all new species described herein.The reports in this study bring the total known luminescent fungi worldwide to 81 species.展开更多
文摘Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.Results Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. Conclusion Human oral keratinocytes have been successfully grown in serial culture.
基金supported by grants PS285/2009B,PV088/2011B and J-21001-76536 by University of Malaya.
文摘Fifteen bioluminescent fungi recently collected from Peninsular Malaysia are analysed herein.The phylogeny of the Malaysian bioluminescent fungi and closely related taxa were evaluated with molecular data from the nuclear ribosomal large subunit(LSU),RNA polymerase 2 second largest subunit(RPB2)and internal transcribed spacers(ITS)gene regions.DNA sequences data support the circumscription of species based on the morphological species concept,but was unable to fully support the current sectional delimitation of genus Mycena.Of the 15 taxa analysed here,four are novel species described herein,viz.Mycena gombakensis,M.nocticaelum,M.coralliformis and Panellus luxfilamentus;while four taxa of Mycena sect.Calodontes were recently described as new.One species(M.noctilucens)represents a new distribution record to Peninsular Malaysia,and six species(Neonothopanus nambi,Filoboletus manipularis,P.luminescens,Roridomyces pruinosoviscidus,M.chlorophos and M.illuminans)have been previously reported.Roridomyces pruinosoviscidus is accepted as a new combination based on morphological and phylogenetic data.Culture morphology data indicate its potential for taxon delimitation as axenic cultures of each species produced unique and distinguishable characteristics.Comprehensive descriptions,illustrations and photographs are provided on basidiome and culture morphology.A key to aid in species identification,comparisons with allied species and data on basidiome and mycelium luminescence are also provided.This study gives the first report on mycelial luminescence for M.noctilucens,P.luminescens,R.pruinosoviscidus as well as all new species described herein.The reports in this study bring the total known luminescent fungi worldwide to 81 species.
