<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. ...<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning </span><span lang="EN-US" style="font-family:;" "="">the </span><span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different <i>in vitro </i>culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant</span><span lang="EN-US" style="font-family:;" "="">,</span><span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (<i>P</i></span><span lang="EN-US" style="font-family:;" "=""> ></span><span lang="EN-US"> </span><span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (<i>P</i> > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (<i>P</i> < 0.05). In contrast, the aforementioned </span><span lang="EN-US" style="font-family:;" "="">result </span><span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (<i>P</i> > 0.05), indicating that the <i>in vitro </i>culture duration is an important factor during <i>in vitro</i> maturation of domestic cat oocytes.</span>展开更多
文摘<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning </span><span lang="EN-US" style="font-family:;" "="">the </span><span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different <i>in vitro </i>culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant</span><span lang="EN-US" style="font-family:;" "="">,</span><span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (<i>P</i></span><span lang="EN-US" style="font-family:;" "=""> ></span><span lang="EN-US"> </span><span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (<i>P</i> > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (<i>P</i> < 0.05). In contrast, the aforementioned </span><span lang="EN-US" style="font-family:;" "="">result </span><span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (<i>P</i> > 0.05), indicating that the <i>in vitro </i>culture duration is an important factor during <i>in vitro</i> maturation of domestic cat oocytes.</span>