This thesis aims at providing an overview of the recent developments and reforms in the college English education. With the employment of multi-media teaching facilities, intercultural competence, i.e., the flexible a...This thesis aims at providing an overview of the recent developments and reforms in the college English education. With the employment of multi-media teaching facilities, intercultural competence, i.e., the flexible adoption of the systems of value outlook and behavior mode of the target language, has indisputably been integrated into the second language learning and teaching curriculum. This thesis compares and analyzes the nature and composition of cultural cognition.展开更多
This paper first summarizes that traditional EFL teaching considers target language isolatedly beyond its culture. It, therefore, stresses the interdependence of language and culture. It analyzes the existing problems...This paper first summarizes that traditional EFL teaching considers target language isolatedly beyond its culture. It, therefore, stresses the interdependence of language and culture. It analyzes the existing problems and their predominant causes in basic EFL teaching, and discusses the possible solutions to be taken to solve such problems. It suggests that there is culture-based instruction for high achievement in classroom acquisition of the target culture. It deems undoubtedly that the systematic permeation of cross-cultural communication aiming at developing cross-cultural awareness is one of the most effective steps toward cultural appropriateness in authentic communication.展开更多
Against the background of a rigorous campaign of globalization of Chinese culture, the global dissemination of local operas has drawn attention from the government, art performers, translators and scholars. This paper...Against the background of a rigorous campaign of globalization of Chinese culture, the global dissemination of local operas has drawn attention from the government, art performers, translators and scholars. This paper takes Wuxi Opera, a precious cultural treasure in Jiangsu province, China, for example. We will discuss the difficulties in spreading Wuxi Opera globally and learn the successful globalizing experience from other operas, such as Peking Opera, Kunqu Opera and Sichuan Opera. Considering the important status and rich resources of Peking Opera, we will analyze Peking Opera thoroughly and the most important aspects of Sichuan Opera and Kunqu Opera. By analyzing their translations and globalization process, we believe that Wuxi Opera should make efforts especially in four aspects to make progress in its globalizing campaign.展开更多
Discourse ideology refers to the position, attitude and guideline employed by a writer or speaker constructing a discourse in relation to the readers/hearers. This paper defines the notion of discourse ideology in the...Discourse ideology refers to the position, attitude and guideline employed by a writer or speaker constructing a discourse in relation to the readers/hearers. This paper defines the notion of discourse ideology in the practice of translation, highlights the relationship between discourse ideology and the translator's subjectivity, and illustrates the translation strategies appropriate to the context of publicity of Chinese minority culture to the readers outside China. A tentative conclusion is drawn that the top-down approach from discourse ideology to translation strategies and the bottom-up approach from translation strategies to discourse ideology complement with each other in the Chinese-to-English translation of publicity on Chinese minority culture.展开更多
It proves to be a tough job to define culture and to draw characteristics of culture or work out the categories of culture. Based on some influential definitions, and achievements made in sociology and anthropology, t...It proves to be a tough job to define culture and to draw characteristics of culture or work out the categories of culture. Based on some influential definitions, and achievements made in sociology and anthropology, this paper makes an attempt to summarize and discuss some characteristics of culture. With regard to the categorization of culture, we put forward the idea that it is closely related to the definition and features of culture, and also that it is closely related with human cognition and different perspectives, and thus culture category is an open system just as culture in general. Cultural development as product of human evolution and development is both a need and reality, going on through cultural transmission. In the process, it is human nature to encounter cultural conflicts like culture shock, the solution of which partially depends on cultural studies, on a close relationship with the main school of culture or dominating culture and on seeking similarities and accepting differences, and in a word, on friendly and cordial contacts and understanding through linguistic communication.展开更多
Today's idea of the so-called fin-de-sibcle is still somewhat inexact and incomplete. This lack of precision originates from a deeply rooted methodological approach which consists of considering the respective develo...Today's idea of the so-called fin-de-sibcle is still somewhat inexact and incomplete. This lack of precision originates from a deeply rooted methodological approach which consists of considering the respective developments of literature, art, and science in isolation, without paying attention to any interference or reciprocal contamination. This defective method also affects the overlaps and contacts between high culture and popular culture. Despite the progress made over recent years, there are few researchers who approach their objective with a real awareness of the complexity of a time period which saw the appearance of mass culture in the current sense and an unprecedented boom in scientific and technological development.1 To this end, the example of the great German philosopher Walter Benjamin and his studies on Paris during the 19th century----collected in the essays on the great French poet Charles Baudelaire and especially in the monumental and unfinished work The Arcades Project--is, without doubt, a model to follow. Taking the path suggested by Benjamin, this article sets out a revision of the novel Bruges-la-Morte (1892) by the Belgian writer Georges Rodenbach, considered one of the major exponents of thefin-de-sikcle decadence, which goes beyond the usual approach of a symbolist reading, by paying special attention to the frictions between literature and technology on one hand, and between literature and popular entertainment on the other.展开更多
A great variety of dairy products with different flavour, texture and health-promoting properties can be obtained from milk using different technologies and starter cultures. Fermented milks are consumed in many parts...A great variety of dairy products with different flavour, texture and health-promoting properties can be obtained from milk using different technologies and starter cultures. Fermented milks are consumed in many parts of the world and are relished for their acidic taste and health benefits. The aim of this study was to compare effects of different ratios of DL-type starter culture: mould Geotrichum candidum grown in milk on its viscosity and sensory properties on the 5th, 10th and 15th day of storage at 4 ~C~ in order to determine the best ratio to prepare fermented milk on basis of Iranian acceptance. We have examined the production of fermented milk by 80: 20, 70: 30, and 60:40 ratios of DL-type starter culture: mould Geotrichum candidum grown in milk. The results obtained from viscosity measurement and sensory evaluation showed no significant difference between these treatments used in the study and were not satisfactory; their viscosity was not accepted and scores attributed to odour and flavor of fermented milks by panelists were low.展开更多
The status and meaning of culture is closely related to the modem China's revolution. Terry Eagleton's cultural derivation provides a basis for redefining culture in the process of Chinese revolution. According to t...The status and meaning of culture is closely related to the modem China's revolution. Terry Eagleton's cultural derivation provides a basis for redefining culture in the process of Chinese revolution. According to this, the new cultural movement in May 4th and the Chinese Marxism aesthetics in Yan'an have taken on the common issues like national and ideological transformation, whereas they have their own historical phase and thus the historical relativity characteristics. As the main body of culture transfer, the intellectuals implement the effective transformation of identity in the process from elite culture to the popularization of culture, and vigorously promote the idea of culture in the context of the Chinese revolution from dilemma to redemption.展开更多
This paper discusses the question of the teaching of culture in an EFL (English as a Foreign Language) class. With the open door policy, an increasing number of people in China are learning English or some other lan...This paper discusses the question of the teaching of culture in an EFL (English as a Foreign Language) class. With the open door policy, an increasing number of people in China are learning English or some other language for various purposes. However, they often experience the frustration of failure in communication with foreigners which can sour relationships or business due to their ignorance of the target culture associated with the language they are learning or have learned. Therefore, teaching culture in foreign language classes and learning a second culture while learning a second language have become new slogans, or to be exact, new tasks in the field of English language teaching in China. But how to teach and what to teach remain problematic. The author holds that "central code" of a culture, i.e., people's way of life should be the focus in EFL teaching. Then choosing appropriate and authentic material containing aspects of culture is of great importance. Greater importance should be attached to mass media and multimedia materials and approaches. At the same time, attention should be paid to the cultural behaviour or belief of EFL teachers so as to make more effective teaching展开更多
The broad and profound Chinese medicine culture is an important part of Chinese traditional culture, which nourishes the vigorous development of modern Chinese medicine. At present, traditional Chinese medicine (TCM...The broad and profound Chinese medicine culture is an important part of Chinese traditional culture, which nourishes the vigorous development of modern Chinese medicine. At present, traditional Chinese medicine (TCM) culture faces challenges and opportunities. As the inheritor of Chinese medicine culture, we bear the burden of inheriting and carrying forward TCM culture. Distinctive community culture is mainly playing value guidance and education transmission function in the process of residents identifying with the culture of Chinese medicine. Therefore, building a distinctive community culture under the guidance of the essence of TCM can effectively inherit and develop TCM culture.展开更多
Observing the effects brought by the universal and individual features of the schema inbred in the different languages and cultures, the author touches on the domesticating and foreignizing methods in the process of t...Observing the effects brought by the universal and individual features of the schema inbred in the different languages and cultures, the author touches on the domesticating and foreignizing methods in the process of translation in cultural background from the perspective of schema theory.展开更多
Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed...Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.展开更多
As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new cul...As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000cells mL^(-1)and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.展开更多
The Five Plague Gods(五瘟神) in the Shuilu(水陆) murals from the Ming and Qing dynasties exemplify a distinctive manifestation of plague gods image developed under the pedigree of Taoist gods.As a significant system f...The Five Plague Gods(五瘟神) in the Shuilu(水陆) murals from the Ming and Qing dynasties exemplify a distinctive manifestation of plague gods image developed under the pedigree of Taoist gods.As a significant system for expelling disasters in Shuilu,the depiction of the Five Plague Gods prominently merges human beings and livestock.This shape form to a large extent,draws on the image of Nuo(傩),a figure reflecting an imagination of the host of plague pathogens.In terms of the numbers of gods,it aligns with the conceptual framework of Wu Xing(五行 the five elements),a foundational principle in traditional Chinese medicine.The internal cultural connection highlights the means of plague prevention and control in China,as well as the external reflection of plague culture.Through multi-dimensional interpretations with the double proof method,this article dissects the formation,core elements,and cultural characteristics of the beliefs in the Five Plague Gods reflected in the Shuilu murals,and transcends mere visual semantics,offering a deeper understanding of these complex cultural symbols.展开更多
Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible...Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.展开更多
Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view...Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view of technology is guided by the concept of he(和)and employs the means and methods of he,thus achieving the purport of he.In Chinese,the character he(和)possesses positive connotations.It originated from the meaning of"to season;to add flavoring to"(调和)and that of flavors being"perfectly harmonious"(和美).From this sensory experience,he gradually extended to the abstract levels of materiality,humanity,sociality,"order"(wei位),and“power,situation,force"(shi势).Finally,he was elevated to the supreme level of"qi of great harmony"(taihe zhi qi太和之气),which is comparable to the concept of dao(道).The philosophy of he has exerted wide impact on such areas as technology,art,national character,cultural psychology,and behavior patterns,and has become an integral part of China's inherent culture.The paradoxes and deviations of he hold their own profound philosophical implications that merit further exploration.As humanity confronts significant challenges,such as how we can coexist with others,with technology,and with nature,the ancient Eastern wisdom embodied in he continues to possess practical characteristics and value.展开更多
The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,an...The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.展开更多
Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound s...Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound system was constructed.The index weights were determined by AHP-entropy method,and the coupling degree,coordinated degree,comprehensive evaluation index and grey correlation degree of MSE system of Mazu Island from 2012 to 2022 were measure.The results showed that:(1)the comprehensive evaluation indexes of the three subsystems was on the rise in general,but the evaluation index of the ecological subsystems increased relatively slowly.(2)The coupling degree was only in the running-in stage in 2012,and the other years were in the coordinated coupling stage.(3)The coupling coordination degree increased from 0.35 in 2012 to 0.82 in 2022,the coupling coordination level was changed from mild imbalance to good coordination.(4)Through the comparison of grey correlation degree,the 24 indexes in the evaluation index system had great influence on the coupling coordination degree of MSE system.The coupling coordination degree was closely related to the development of socio-economy and the spread of Mazu culture.With the rapid development of tourism brought about by the spread of Mazu culture,the pressure on the ecological environment will be increasing.Compared with the rapid growth of tourism and economy,it is equally important to strengthen environmental protection and pay attention to the quality of ecological environment development.展开更多
Based on output-oriented education,the OBE(Outcome-Based Education)concept integrates local red culture into the ideological and political course of environmental disciplines,and is an important part of training appli...Based on output-oriented education,the OBE(Outcome-Based Education)concept integrates local red culture into the ideological and political course of environmental disciplines,and is an important part of training applied talents of environmental disciplines in the new era.This educational model makes an innovation on the traditional educational and teaching concepts and centers on students.This paper analyzes the value of integrating local red culture into the ideological and political course under the OBE concept,and puts forward an effective implementation path.展开更多
The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the ...The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the historical path of archaeological discoveries,from the first Trypillian sanctuaries to the discovery of the largest Nebelivka temple complex in Ukraine in 2012.At the same time,the work partially raises the issue of religious beliefs of the ancient farmers of Central Europe in connection with the discovery of their sacred buildings.Since special scientific works have not comprehensively addressed this issue and are still fragmentary in nature,it is important at the present stage of research to make some coverage and systematization of existing materials on this issue.展开更多
文摘This thesis aims at providing an overview of the recent developments and reforms in the college English education. With the employment of multi-media teaching facilities, intercultural competence, i.e., the flexible adoption of the systems of value outlook and behavior mode of the target language, has indisputably been integrated into the second language learning and teaching curriculum. This thesis compares and analyzes the nature and composition of cultural cognition.
文摘This paper first summarizes that traditional EFL teaching considers target language isolatedly beyond its culture. It, therefore, stresses the interdependence of language and culture. It analyzes the existing problems and their predominant causes in basic EFL teaching, and discusses the possible solutions to be taken to solve such problems. It suggests that there is culture-based instruction for high achievement in classroom acquisition of the target culture. It deems undoubtedly that the systematic permeation of cross-cultural communication aiming at developing cross-cultural awareness is one of the most effective steps toward cultural appropriateness in authentic communication.
文摘Against the background of a rigorous campaign of globalization of Chinese culture, the global dissemination of local operas has drawn attention from the government, art performers, translators and scholars. This paper takes Wuxi Opera, a precious cultural treasure in Jiangsu province, China, for example. We will discuss the difficulties in spreading Wuxi Opera globally and learn the successful globalizing experience from other operas, such as Peking Opera, Kunqu Opera and Sichuan Opera. Considering the important status and rich resources of Peking Opera, we will analyze Peking Opera thoroughly and the most important aspects of Sichuan Opera and Kunqu Opera. By analyzing their translations and globalization process, we believe that Wuxi Opera should make efforts especially in four aspects to make progress in its globalizing campaign.
文摘Discourse ideology refers to the position, attitude and guideline employed by a writer or speaker constructing a discourse in relation to the readers/hearers. This paper defines the notion of discourse ideology in the practice of translation, highlights the relationship between discourse ideology and the translator's subjectivity, and illustrates the translation strategies appropriate to the context of publicity of Chinese minority culture to the readers outside China. A tentative conclusion is drawn that the top-down approach from discourse ideology to translation strategies and the bottom-up approach from translation strategies to discourse ideology complement with each other in the Chinese-to-English translation of publicity on Chinese minority culture.
文摘It proves to be a tough job to define culture and to draw characteristics of culture or work out the categories of culture. Based on some influential definitions, and achievements made in sociology and anthropology, this paper makes an attempt to summarize and discuss some characteristics of culture. With regard to the categorization of culture, we put forward the idea that it is closely related to the definition and features of culture, and also that it is closely related with human cognition and different perspectives, and thus culture category is an open system just as culture in general. Cultural development as product of human evolution and development is both a need and reality, going on through cultural transmission. In the process, it is human nature to encounter cultural conflicts like culture shock, the solution of which partially depends on cultural studies, on a close relationship with the main school of culture or dominating culture and on seeking similarities and accepting differences, and in a word, on friendly and cordial contacts and understanding through linguistic communication.
文摘Today's idea of the so-called fin-de-sibcle is still somewhat inexact and incomplete. This lack of precision originates from a deeply rooted methodological approach which consists of considering the respective developments of literature, art, and science in isolation, without paying attention to any interference or reciprocal contamination. This defective method also affects the overlaps and contacts between high culture and popular culture. Despite the progress made over recent years, there are few researchers who approach their objective with a real awareness of the complexity of a time period which saw the appearance of mass culture in the current sense and an unprecedented boom in scientific and technological development.1 To this end, the example of the great German philosopher Walter Benjamin and his studies on Paris during the 19th century----collected in the essays on the great French poet Charles Baudelaire and especially in the monumental and unfinished work The Arcades Project--is, without doubt, a model to follow. Taking the path suggested by Benjamin, this article sets out a revision of the novel Bruges-la-Morte (1892) by the Belgian writer Georges Rodenbach, considered one of the major exponents of thefin-de-sikcle decadence, which goes beyond the usual approach of a symbolist reading, by paying special attention to the frictions between literature and technology on one hand, and between literature and popular entertainment on the other.
文摘A great variety of dairy products with different flavour, texture and health-promoting properties can be obtained from milk using different technologies and starter cultures. Fermented milks are consumed in many parts of the world and are relished for their acidic taste and health benefits. The aim of this study was to compare effects of different ratios of DL-type starter culture: mould Geotrichum candidum grown in milk on its viscosity and sensory properties on the 5th, 10th and 15th day of storage at 4 ~C~ in order to determine the best ratio to prepare fermented milk on basis of Iranian acceptance. We have examined the production of fermented milk by 80: 20, 70: 30, and 60:40 ratios of DL-type starter culture: mould Geotrichum candidum grown in milk. The results obtained from viscosity measurement and sensory evaluation showed no significant difference between these treatments used in the study and were not satisfactory; their viscosity was not accepted and scores attributed to odour and flavor of fermented milks by panelists were low.
文摘The status and meaning of culture is closely related to the modem China's revolution. Terry Eagleton's cultural derivation provides a basis for redefining culture in the process of Chinese revolution. According to this, the new cultural movement in May 4th and the Chinese Marxism aesthetics in Yan'an have taken on the common issues like national and ideological transformation, whereas they have their own historical phase and thus the historical relativity characteristics. As the main body of culture transfer, the intellectuals implement the effective transformation of identity in the process from elite culture to the popularization of culture, and vigorously promote the idea of culture in the context of the Chinese revolution from dilemma to redemption.
文摘This paper discusses the question of the teaching of culture in an EFL (English as a Foreign Language) class. With the open door policy, an increasing number of people in China are learning English or some other language for various purposes. However, they often experience the frustration of failure in communication with foreigners which can sour relationships or business due to their ignorance of the target culture associated with the language they are learning or have learned. Therefore, teaching culture in foreign language classes and learning a second culture while learning a second language have become new slogans, or to be exact, new tasks in the field of English language teaching in China. But how to teach and what to teach remain problematic. The author holds that "central code" of a culture, i.e., people's way of life should be the focus in EFL teaching. Then choosing appropriate and authentic material containing aspects of culture is of great importance. Greater importance should be attached to mass media and multimedia materials and approaches. At the same time, attention should be paid to the cultural behaviour or belief of EFL teachers so as to make more effective teaching
文摘The broad and profound Chinese medicine culture is an important part of Chinese traditional culture, which nourishes the vigorous development of modern Chinese medicine. At present, traditional Chinese medicine (TCM) culture faces challenges and opportunities. As the inheritor of Chinese medicine culture, we bear the burden of inheriting and carrying forward TCM culture. Distinctive community culture is mainly playing value guidance and education transmission function in the process of residents identifying with the culture of Chinese medicine. Therefore, building a distinctive community culture under the guidance of the essence of TCM can effectively inherit and develop TCM culture.
文摘Observing the effects brought by the universal and individual features of the schema inbred in the different languages and cultures, the author touches on the domesticating and foreignizing methods in the process of translation in cultural background from the perspective of schema theory.
基金partly funded by the Department of Science and Technology Fund for Improvement of S&T Infrastructure (Grant No. SR/FST/LS-I/2018/125)。
文摘Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.
基金supported by the National Natural Science Foundation of China (No.U22A20588)the Sanya Science and Technology Project (No.2022KJCX57)+1 种基金the Qingdao National Laboratory for Marine Science and Technology (No.12-04)the Project supported by the Education Department of Hainan Province (No.Hnjg2024276)。
文摘As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000cells mL^(-1)and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.
基金financed by the grants from Shanxi Province Postgraduate Education Innovation Plan (No. 2023KY126)Humanities and Social Sciences Research Project of The Ministry of Ecuation (No. 23YJAZH182)。
文摘The Five Plague Gods(五瘟神) in the Shuilu(水陆) murals from the Ming and Qing dynasties exemplify a distinctive manifestation of plague gods image developed under the pedigree of Taoist gods.As a significant system for expelling disasters in Shuilu,the depiction of the Five Plague Gods prominently merges human beings and livestock.This shape form to a large extent,draws on the image of Nuo(傩),a figure reflecting an imagination of the host of plague pathogens.In terms of the numbers of gods,it aligns with the conceptual framework of Wu Xing(五行 the five elements),a foundational principle in traditional Chinese medicine.The internal cultural connection highlights the means of plague prevention and control in China,as well as the external reflection of plague culture.Through multi-dimensional interpretations with the double proof method,this article dissects the formation,core elements,and cultural characteristics of the beliefs in the Five Plague Gods reflected in the Shuilu murals,and transcends mere visual semantics,offering a deeper understanding of these complex cultural symbols.
基金support from the National Key Research and Development Program of China(Grant No.2017YFA0700501),and the Innovation Fund of WNLO.
文摘Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.
基金the support of the Center for Cultural Studies on Science and Technology in China at Technische Universitat Berlin。
文摘Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view of technology is guided by the concept of he(和)and employs the means and methods of he,thus achieving the purport of he.In Chinese,the character he(和)possesses positive connotations.It originated from the meaning of"to season;to add flavoring to"(调和)and that of flavors being"perfectly harmonious"(和美).From this sensory experience,he gradually extended to the abstract levels of materiality,humanity,sociality,"order"(wei位),and“power,situation,force"(shi势).Finally,he was elevated to the supreme level of"qi of great harmony"(taihe zhi qi太和之气),which is comparable to the concept of dao(道).The philosophy of he has exerted wide impact on such areas as technology,art,national character,cultural psychology,and behavior patterns,and has become an integral part of China's inherent culture.The paradoxes and deviations of he hold their own profound philosophical implications that merit further exploration.As humanity confronts significant challenges,such as how we can coexist with others,with technology,and with nature,the ancient Eastern wisdom embodied in he continues to possess practical characteristics and value.
基金supported by the National Key Research and Development Program of China(2021YFD2100902-3)the National Natural Science Foundation of China(32072258)+5 种基金Major Science and Technology Program of Heilongjiang(2020ZX08B02)Harbin University of Commerce“Young Innovative Talents”Support Program(2019CX062020CX262020CX27)the Central Financial Support for the Development of Local Colleges and Universities,Graduate Innovation Research Project of Harbin University of Commerce(YJSCX2021-698HSD)Training plan of Young Innovative Talents in Universities of Heilongjiang(UNPYSCT-2020218).
文摘The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.
基金This paper is supported by the National Natural Science Foundation of China(Grant No.31400318)the Fujian Provincial Department of Science and Technology Guided Projects(Grant No.2020Y0089)the STS Project of Fujian Science and Technology Department(Grant Nos.2021T3014,2022T3023).
文摘Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound system was constructed.The index weights were determined by AHP-entropy method,and the coupling degree,coordinated degree,comprehensive evaluation index and grey correlation degree of MSE system of Mazu Island from 2012 to 2022 were measure.The results showed that:(1)the comprehensive evaluation indexes of the three subsystems was on the rise in general,but the evaluation index of the ecological subsystems increased relatively slowly.(2)The coupling degree was only in the running-in stage in 2012,and the other years were in the coordinated coupling stage.(3)The coupling coordination degree increased from 0.35 in 2012 to 0.82 in 2022,the coupling coordination level was changed from mild imbalance to good coordination.(4)Through the comparison of grey correlation degree,the 24 indexes in the evaluation index system had great influence on the coupling coordination degree of MSE system.The coupling coordination degree was closely related to the development of socio-economy and the spread of Mazu culture.With the rapid development of tourism brought about by the spread of Mazu culture,the pressure on the ecological environment will be increasing.Compared with the rapid growth of tourism and economy,it is equally important to strengthen environmental protection and pay attention to the quality of ecological environment development.
基金Supported by Teaching Content and Curriculum System Reform Project of Guizhou Province in 2022(GZJG20220776)Natural Science Research Project of Guizhou Provincial Department of Education(Qianjiaoji[2022]No.067)+1 种基金Research Center for Revolutionary Spirit and Cultural Resources of the Communist Party of China,Zunyi Normal University,Key Research Base of Humanities and Social Sciences,Ministry of Education(22KRIZYPY12)Teaching Content and Curriculum System Reform and Cultivation Project of Zunyi Normal University in 2022(JGPY2022001).
文摘Based on output-oriented education,the OBE(Outcome-Based Education)concept integrates local red culture into the ideological and political course of environmental disciplines,and is an important part of training applied talents of environmental disciplines in the new era.This educational model makes an innovation on the traditional educational and teaching concepts and centers on students.This paper analyzes the value of integrating local red culture into the ideological and political course under the OBE concept,and puts forward an effective implementation path.
文摘The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the historical path of archaeological discoveries,from the first Trypillian sanctuaries to the discovery of the largest Nebelivka temple complex in Ukraine in 2012.At the same time,the work partially raises the issue of religious beliefs of the ancient farmers of Central Europe in connection with the discovery of their sacred buildings.Since special scientific works have not comprehensively addressed this issue and are still fragmentary in nature,it is important at the present stage of research to make some coverage and systematization of existing materials on this issue.