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Indoor Toxicity of Emamectin Benzoate and Chlorantraniliprole Against Spodoptera litura and Their Residues on Lotus Plants
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作者 CUI Kan XU Zhi-de +5 位作者 LI Kai-long CHEN Wu-ying HU Ya-hui XIAO Jian-ping HAO Xiao-bin WEI Lin 《Agricultural Science & Technology》 CAS 2024年第3期44-49,共6页
Spodoptera litura is the most threatening pest in lotus production,seriously affecting the lotus yield and quality.Emamectin benzoate and chlorantraniliprole,the main insecticides for controlling S.litura on vegetable... Spodoptera litura is the most threatening pest in lotus production,seriously affecting the lotus yield and quality.Emamectin benzoate and chlorantraniliprole,the main insecticides for controlling S.litura on vegetables,are widely used by farmers to control S.litura on lotus plants.To determine the application concentrations,control effects,and safety of the two insecticides in lotus fields,indoor experiments were conducted to determine the control effects of 200 g/L chlorantraniliprole(SC)and 5%emamectin benzoate(WDG),and the residues of the two insecticides in the water,lotus leaves,and lotus seeds after field application were determined by HPLC-MS/MS.The indoor experiment results showed that chlorantraniliprole and emamectin benzoate both had good control effects on S.litura,with the median lethal concentrations(LC50)of 17.700 and 1.694 mg/L,respectively.After unmanned aerial vehicle spraying of emamectin benzoate at 20 g/667m^(2),there was no residue of emamectin benzoate in the water or lotus leaves after 5 d.After spraying of chlorantraniliprole at 20 mL/667m^(2),the residual amounts in the water and lotus leaves after 9 d were 0.005 and 0.007 mg/L,respectively.No residue of the two insecticides was detected in lotus seeds(dry and fresh)2 h after spraying.Therefore,it was recommended that chlorantraniliprole and emamectin benzoate can be used to control S.litura in lotus fields during the growth period,while attention should be paid to the application interval for safety.Considering the safe harvesting of lotus seeds and leaves,it was recommended that the preharvest intervals of chlorantraniliprole and 5%emamectin benzoate should be 9 d. 展开更多
关键词 cunsanlian Spodoptera litura Emamectin benzoate CHLORANTRANILIPROLE Insecticide residue
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莲中介导矿质元素胁迫耐受性基因的筛选与功能验证
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作者 胡衡亮 顾天宇 +2 位作者 陈思颖 王垚 彭佳师 《中国农业科学》 CAS CSCD 北大核心 2024年第5期980-988,共9页
【目的】莲是一种传统的药食同源作物,但其蕴藏的丰富基因资源仍缺乏功能鉴定。分离鉴定莲中参与微量矿质营养元素及重金属元素积累或耐受过程的功能基因,丰富莲中介导营养高效以及逆境耐受的基因资源,并为莲的遗传改良提供理论依据。... 【目的】莲是一种传统的药食同源作物,但其蕴藏的丰富基因资源仍缺乏功能鉴定。分离鉴定莲中参与微量矿质营养元素及重金属元素积累或耐受过程的功能基因,丰富莲中介导营养高效以及逆境耐受的基因资源,并为莲的遗传改良提供理论依据。【方法】以湘莲代表品种‘寸三莲’为试验材料。首先构建莲的酵母表达cDNA文库,并将文库转化酿酒酵母后分别在含有过量镉(Cd)、锰(Mn)、锌(Zn)、铜(Cu)、铁(Fe)、铝(Al)胁迫的平板上筛选,分离阳性酵母克隆中介导相关胁迫耐受性的目的基因。结合生物信息学分析和酵母互补试验对筛选出的耐受基因进行功能验证。【结果】构建了库容量超过106个单克隆、重组率100%、平均插入片段大于1 000 bp的酵母文库。通过筛选得到介导Cd耐受的基因13个、Mn耐受基因4个、Zn耐受基因4个、Cu耐受基因3个、Fe耐受基因7个、Al耐受基因1个,其中3个基因能够同时介导Fe和Mn的耐受性。这些基因在莲除6号染色外的其他染色体上都有分布。【结论】本研究构建了高质量的莲酵母表达cDNA文库,并筛选得到29个介导微量矿质营养元素或重金属元素胁迫抗性的基因,为莲的营养高效以及避免重金属污染的遗传改良积累了基因资源。 展开更多
关键词 寸三莲 矿质营养元素 重金属 抗性基因
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莲种子萌发及幼苗生长过程中抗氧化酶活性的变化 被引量:5
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作者 张曼 裴康康 孔德政 《河南科学》 2010年第5期547-549,共3页
以"寸三莲"种子为例,详细分析了其萌发及幼苗生长过程中,子叶和胚中抗氧化酶活性的变化."寸三莲"种子在正常的萌发条件下,SOD活性变化比较平缓,且子叶和胚中SOD活性变化趋势一致,均为先上升后下降,这可能与萌发过... 以"寸三莲"种子为例,详细分析了其萌发及幼苗生长过程中,子叶和胚中抗氧化酶活性的变化."寸三莲"种子在正常的萌发条件下,SOD活性变化比较平缓,且子叶和胚中SOD活性变化趋势一致,均为先上升后下降,这可能与萌发过程中氧自由基含量的变化有关;在整个发芽进程中,POD活性远远高于子叶POD活性;CAT活性相对稳定,而胚中CAT活性变化较大. 展开更多
关键词 “寸三莲”种子 萌发 超氧化物歧化酶 过氧化物酶 过氧化氢酶
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