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我带领的中国足球队——《2002 FIFA World Cup》之旅
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《计算机与网络》 2002年第11期22-24,共3页
转眼间四年又过去了,世界杯这道足球大菜又要“上桌”了。今年的世界杯因为众多球星的受伤而比四年前更加富有悬念性。对于中国球迷来说,因为这界世界杯是中国足球44年来首次打进决赛圈的一届,因而更加受到中国球迷的关注。在世界杯举... 转眼间四年又过去了,世界杯这道足球大菜又要“上桌”了。今年的世界杯因为众多球星的受伤而比四年前更加富有悬念性。对于中国球迷来说,因为这界世界杯是中国足球44年来首次打进决赛圈的一届,因而更加受到中国球迷的关注。在世界杯举办的同时,其他领域的众多商家们各显神通,推出各式各样的产品让球迷来分享世界杯这道大菜。作为体育游戏领域中的“大腕”EA sports不失时机地推出了以世界杯为主题的游戏《2002FIFA World cup》,使那些球迷玩家能提前过一把世界杯的瘾。 展开更多
关键词 《2002 FIFA World cup》 旅游软件 电脑游戏
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Refined Anam-Net:Lightweight Deep Learning Model for Improved Segmentation Performance of Optic Cup and Disc for Glaucoma Diagnosis
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作者 Khursheed Aurangzeb Syed Irtaza Haider Musaed Alhussein 《Computers, Materials & Continua》 SCIE EI 2024年第7期1381-1405,共25页
In this work,we aim to introduce some modifications to the Anam-Net deep neural network(DNN)model for segmenting optic cup(OC)and optic disc(OD)in retinal fundus images to estimate the cup-to-disc ratio(CDR).The CDR i... In this work,we aim to introduce some modifications to the Anam-Net deep neural network(DNN)model for segmenting optic cup(OC)and optic disc(OD)in retinal fundus images to estimate the cup-to-disc ratio(CDR).The CDR is a reliable measure for the early diagnosis of Glaucoma.In this study,we developed a lightweight DNN model for OC and OD segmentation in retinal fundus images.Our DNN model is based on modifications to Anam-Net,incorporating an anamorphic depth embedding block.To reduce computational complexity,we employ a fixed filter size for all convolution layers in the encoder and decoder stages as the network deepens.This modification significantly reduces the number of trainable parameters,making the model lightweight and suitable for resource-constrained applications.We evaluate the performance of the developed model using two publicly available retinal image databases,namely RIM-ONE and Drishti-GS.The results demonstrate promising OC segmentation performance across most standard evaluation metrics while achieving analogous results for OD segmentation.We used two retinal fundus image databases named RIM-ONE and Drishti-GS that contained 159 images and 101 retinal images,respectively.For OD segmentation using the RIM-ONE we obtain an f1-score(F1),Jaccard coefficient(JC),and overlapping error(OE)of 0.950,0.9219,and 0.0781,respectively.Similarly,for OC segmentation using the same databases,we achieve scores of 0.8481(F1),0.7428(JC),and 0.2572(OE).Based on these experimental results and the significantly lower number of trainable parameters,we conclude that the developed model is highly suitable for the early diagnosis of glaucoma by accurately estimating the CDR. 展开更多
关键词 Refined Anam-Net parameter tuning deep learning optic cup optic disc cup-to-disc ratio glaucoma diagnosis
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Study of plasma parameters of coaxial plasma source using triple Langmuir probe and Faraday cup diagnostics
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作者 Sunil KANCHI Rohit SHUKLA Archana SHARMA 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第4期94-100,共7页
Coaxial plasma guns are a type of plasma source that produces plasma which propagates radially and axially controlled by the shape of the ground electrode, which has attracted much interest in several applications. In... Coaxial plasma guns are a type of plasma source that produces plasma which propagates radially and axially controlled by the shape of the ground electrode, which has attracted much interest in several applications. In this work, a 120° opening angle of CPG nozzle is used as a plasma gun configuration that operates at the energy of 150 J. The ionization of polyethylene insulator between the electrodes of the gun produces a cloud of hydrogen and carbon plasma.The triple Langmuir probe and Faraday cup are used to measure plasma density and plasma temperature. These methods are used to measure the on-axis and off-axis plasma divergence of the coaxial plasma gun. The peak values of ion densities measured at a distance of 25 mm on-axis from the plasma gun are(1.6±0.5)×10^(19)m^(-3)and(2.8±0.6)×10^(19)m^(-3)for hydrogen and carbon plasma respectively and the peak temperature is 3.02±0.5 eV. The mean propagation velocity of plasma is calculated using the transit times of plasma at different distances from the plasma gun and is found to be 4.54±0.25 cm/μs and 1.81±0.18 cm/μs for hydrogen and carbon plasma respectively. The Debye radius is obtained from the measured experimental data that satisfies the thin sheath approximation. The shot-to-shot stability of plasma parameters facilitates the use of plasma guns in laboratory experiments. These types of plasma sources can be used in many applications like plasma opening switches, plasma devices, and as plasma sources. 展开更多
关键词 coaxial plasma source triple Langmuir probe Faraday cup plasma density plasma temperature
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Mid-term clinical outcomes of the uncemented Robert Mathys pressfit cup
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作者 Kishan Ritek Ramsodit Inger N Sierevelt +2 位作者 Esther R C Janssen Sjoerd Kaarsemaker Daniël Haverkamp 《World Journal of Clinical Cases》 SCIE 2024年第19期3684-3691,共8页
BACKGROUND The use of uncemented cups during total hip arthroplasty(THA)has gained popularity in recent years.The Robert Mathys(RM)pressfit cup,an uncemented monoblock implant is expected to preserve bone density due ... BACKGROUND The use of uncemented cups during total hip arthroplasty(THA)has gained popularity in recent years.The Robert Mathys(RM)pressfit cup,an uncemented monoblock implant is expected to preserve bone density due to its composition and external surface,while reducing backside wear with its monoblock construction.These factors should lead to a high survival rate of the implant.AIM To evaluate the mid-term survival and functional outcome of the RM Pressfit cup in a large study population.METHODS Between 2011 and 2020,we included 1324 patients receiving a primary THA using the RM pressfit cup.Final clinical follow-up was performed at 2 years postoperatively with the Dutch arthroplasty register used to assess implant status thereafter.Revision for acetabular failure and reason for revision were reported to evaluate implant survival,while the hip disability and osteoarthritis outcome score(HOOS)scores were used to assess functional outcome.RESULTS The mean age at surgery was 64.9 years.The mean follow-up was 4.6 years.Of the 1324 THAs performed,13 needed cup revisions within 5 years after index THA:5 due to aseptic loosening,6 due to infection,2 due to dislocation and 2 due to other causes.This resulted in a 5-year cup survival of 98.8%(95%CI:98.1-99.5).Nine of the cup revisions occurred within the first year after index THA.HOOS scores increased significantly in all domains during the first year and levelled out during the second year.CONCLUSION In the present study,the RM pressfit cup demonstrated excellent clinical outcomes at mid-term follow-up;however,future studies are needed to assess the long-term outcomes of this acetabular implant. 展开更多
关键词 Total hip arthroplasty Uncemented RM pressfit cup Hip osteoarthritis cup revision Pressfit Aseptic loosening
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Enhancing the Decomposition of Paper Cups Using Galleria Mellonella and Eisenia Fetida
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作者 Shadi Moqbel Habib Al-Ghoul +1 位作者 Abd Al-Majeed Al-Ghzawi Rami Mukbel 《Journal of Renewable Materials》 EI CAS 2024年第2期349-367,共19页
The composition of paper cups creates a challenge for the recycling industry,as the paperboard–plastic film composite is hard to separate.Therefore,paper cups are sent to landfills or waste incinerators.This study ex... The composition of paper cups creates a challenge for the recycling industry,as the paperboard–plastic film composite is hard to separate.Therefore,paper cups are sent to landfills or waste incinerators.This study explores the combined use of red worms(Eisenia fetida)and Greater wax moth(Galleria mellonella)in the biodegradation of paper cups.The study investigates the conditions and combinations that promote using Eisenia fetida and Galleria mellonella for degrading paper cups.The study considered the influence of environmental temperature,the presence of food waste,varying the number of Eisenia fetida worms,and the presence of a Galleria mellonella growth-slowing agent on the degradation process.To achieve the study objectives,the study followed a quantitative approach.The study monitored the degradation of paper cup cuts that were placed in jars containing different combinations of Eisenia fetida worms,Galleria mellonella larvae,food waste,bedding material,and Galleria mellonella growth-slowing agents.The study found that the best operating temperature is 30oC.The study found that using food waste improves the performance of Eisenia fetida worms and Galleria mellonella larvae significantly.The study found that adding a Galleria mellonella growth-slowing agent slightly enhances the degradation of the paper cup.Finally,a numerical model was obtained to simulate the paper cup degradation efficiency. 展开更多
关键词 Paper cups resource recovery RECYCLING circular economy Galleria mellonella Eisenia fetida
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Acetabular cup size trends in total hip arthroplasty
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作者 Daniel Patrick McKenna Alex Price +3 位作者 Timothy McAleese Darren Dahly Paul McKenna May Cleary 《World Journal of Orthopedics》 2024年第1期39-44,共6页
BACKGROUND Total hip arthroplasty(THA)is a common procedure for end stage osteoarthritis.The learning curve for THA is complex and challenging.One of the most difficult skills to master is acetabular reaming.We wish t... BACKGROUND Total hip arthroplasty(THA)is a common procedure for end stage osteoarthritis.The learning curve for THA is complex and challenging.One of the most difficult skills to master is acetabular reaming.We wish to identify if experience in arthroplasty leads to preservation of more bone stock.AIM To investigate if increasing surgeon experience will predict an ever decreasing acetabular cup size.METHODS A retrospective case series of four attending orthopaedic surgeons was completed.All uncemented elective total hip arthroplasties since appointment were selected for inclusion.The size of acetabular cup used was noted and logistic regression was used to identify if a trend to smaller cups existed.RESULTS A total of 1614 subjects were included with a mean age of 64 years.Overall cups were on average 0.18mm smaller per year(95%confidence interval-0.25 to-0.11,P<0.001).Individual surgeon trends showed cup sizes to decrease 0.27 mm/year for surgeon A,0.02 mm/year for surgeon B,0.15 mm/year for surgeon C and 0.29 mm/year for surgeon D.Three of the four surgeons had a more pronounced trend to smaller cups for male subjects than their female counterparts.CONCLUSION We found increasing surgeon experience to be associated with an ever-decreasing acetabular cup size.Smaller acetabular cup size may act as a surrogate marker of surgical proficiency by virtue of decreased acetabular reaming. 展开更多
关键词 HIP ARTHROPLASTY ACETABULUM cup Learning
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Physical and Chemical Characterizations of Rubber Latex Cup Bottom Oil
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作者 Kouassi Konan Edmond Abolle Abollé +3 位作者 Aketchi Tanoé Lucien Konan Affoué Tindo Sylvie Boa David Yao Kouassi Benjamin 《Advances in Bioscience and Biotechnology》 CAS 2024年第9期511-521,共11页
Rubber latex is an important economic resource. However, the residues from its harvesting are thrown away, even though they contain lipids that can be recycled. This recovery of the residues from the bottom of the cup... Rubber latex is an important economic resource. However, the residues from its harvesting are thrown away, even though they contain lipids that can be recycled. This recovery of the residues from the bottom of the cup requires first and foremost their characterization. The aim of this study is therefore to determine the main physical and chemical characteristics of rubber latex cup bottom oil. Oil’s physical parameters determination shows that it has a density of 951 kg∙m−3, a kinematic viscosity of 48.57 cSt and a water content of 0.0845%. Chemical parameters, meanwhile, indicate that this cup bottom residue has a fat content of 95.96%, an acid number of 2.805 mg KOH/g and an iodine number of 92.42 g I2/100g. Therefore, rubber latex cup bottom oil can be used in the formulation of biofuels, biolubricants, paints, varnishes, alkyd resins, polishing oils, soaps, and insecticides. 展开更多
关键词 Rubber Latex cup Bottoms Oil Density Viscosity CHARACTERIZATIONS
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基于CUPS的国产操作系统打印管控的应用研究
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作者 张青岭 张艳 +1 位作者 石春刚 张众维 《天津城建大学学报》 CAS 2024年第1期66-71,共6页
随着信息技术的快速发展和信息安全防护工作要求的不断提高,我国一些企事业单位正在逐步使用国产操作系统,在其上的打印管控已成为不可缺少的一部分,但目前适用于国产操作系统的打印系统尚未成熟.针对此问题,首先研究了通用Unix打印系统... 随着信息技术的快速发展和信息安全防护工作要求的不断提高,我国一些企事业单位正在逐步使用国产操作系统,在其上的打印管控已成为不可缺少的一部分,但目前适用于国产操作系统的打印系统尚未成熟.针对此问题,首先研究了通用Unix打印系统(common unix printing system,CUPS)原理,然后针对国产操作系统提出基于CUPS架构的打印系统,设计打印机驱动,并将设计的打印系统部署在国产操作系统中,实现了在国产中科方德操作系统上的打印管控.测试结果显示其效率高、体积小、方便快捷,具有较强的应用价值. 展开更多
关键词 cupS 国产操作系统 打印机驱动 打印管控
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The European Cup Has Limited Effect to “Save” Europe
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作者 Li Si 《China's Foreign Trade》 2024年第4期52-53,共2页
On June 15th,the one-month European Football Championship(hereinafter referred to as the European Cup)kicked off in Germany.In 2023,Germany’s GDP shrank by 0.3%,which was the worst performance among the world’s majo... On June 15th,the one-month European Football Championship(hereinafter referred to as the European Cup)kicked off in Germany.In 2023,Germany’s GDP shrank by 0.3%,which was the worst performance among the world’s major economies.This year,Germany is pinning its hopes on the European Championship to stimulate consumption and boost the economy. 展开更多
关键词 European cup BOOST
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A meta-analysis on the clinical efficacy of fire dragon cupping in the treatment of lumbar disc herniation
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作者 Hai-Xia Ma Dan Ma +1 位作者 Bin Lin Juan-Xia Zheng 《Integrative Medicine Discovery》 2024年第11期1-7,共7页
Background:The incidence of lumbar disc herniation(LDH)is notably high.Consensus among experts highlights non-surgical treatments as the primary therapeutic approach for LDH.Contemporary medicine frequently employs ph... Background:The incidence of lumbar disc herniation(LDH)is notably high.Consensus among experts highlights non-surgical treatments as the primary therapeutic approach for LDH.Contemporary medicine frequently employs pharmacotherapy and epidural injections in such treatments,which are associated with numerous adverse effects.Prolonged use can severely impair the liver and kidney functions of patients.Hence,the role of safe and effective traditional Chinese medicine techniques becomes pivotal.Among various traditional Chinese medicine approaches for treating LDH,fire dragon cupping,renowned for its remarkable efficacy,cost-effectiveness,and ease of application,is extensively utilised in clinical settings for managing LDH.Nonetheless,there is a scarcity of systematic and standardised evidence from evidence-based medicine studies.Therefore,conducting a meta-analysis is imperative.Methods:A comprehensive computerised search was conducted in databases including China National Knowledge Infrastructure,WanFang Data,VIP,China Biology Medicine disc,PubMed,EMbase,The Cochrane Library,Web of Science,and CINAHL.The search aimed to gather randomised controlled trials on fire dragon cupping therapy for LDH,spanning from the inception of these databases until December 2023.Two researchers independently screened the literature according to inclusion and exclusion criteria,extracted data,and assessed the methodological quality of the studies included,utilising RevMan 5.3 software for meta-analysis.Results:The results show that the fire dragon cupping therapy group had better clinical effectiveness(relative risk=1.23,95%confidence interval(CI)(1.14,1.33),P<0.00001),less pain(standardized mean difference=–1.33,95%CI(–1.49,–1.16),P<0.00001),and some improvement in lumbar function(Japanese Orthopaedic Association scores:mean difference=3.37,95%CI(2.31,4.43),P<0.00001.Conclusion:The fire dragon cupping therapy significantly alleviates LDH,warranting its extensive application.However,considering the limitations in the number and quality of studies included,the aforementioned conclusion necessitates further validation through more high-quality research. 展开更多
关键词 fire dragon cupping therapy lumbar disc herniation lumbar spine META-ANALYSIS randomized controlled trial
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Observation on Therapeutic Effect of Lotus Needle Cupping Therapy for Removing Blood Stasis in Zhuang Medicine on PHN and Its Influ-ence on Inflammatory Mediators
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作者 Liping QIN Yulu WEI +4 位作者 Faming SHU Mingfu PAN Zujie QIN Da WEI Jiaojiao HUANG 《Medicinal Plant》 2024年第5期61-65,共5页
[Objectives]To observe the clinical effect of the lotus needle cupping therapy for removing blood stasis in Zhuang medicine in treating PHNand its influence on inflammatory factors.[Methods]96 patients with PHN were r... [Objectives]To observe the clinical effect of the lotus needle cupping therapy for removing blood stasis in Zhuang medicine in treating PHNand its influence on inflammatory factors.[Methods]96 patients with PHN were randomly divided into three groups:lotus nee-dle cupping therapy group,TCM surrounding acupuncture group and gabapentin group.Venous blood and acupoint blood were collected at 0,21 and 42 d of treatment,and the expression levels of 5-HT,SP and CGRPinflammatory mediators were detected before and after treatment.The changes of VAS scores before,during and after treatment and the clinical efficacy were observed.[Results]The total effective rate of the lotus needle cupping group was 87.50%,which was better than that of the TCM acupuncture group(81.25%)and the gabapentin group(62.50%);after treatment,the VAS scores and the expression of inflammatory mediators in the three groups of patients were lower than those before treatment,and the decrease was more significant in the treatment group(P<0.05).[Conclusions]The lotus needle cupping therapy for removing blood stasis in Zhuang medicine is effective in treating PHN,and its mechanism is to reduce the release of inflammatory media-tors,reduce hyperalgesia,relieve pain and improve the quality of life of patients. 展开更多
关键词 Lotus needle cupping therapy for removing blood stasis in Zhuang medicine Postherpetic neuralgia Inflammatory mediator
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Subconjunctival hemorrhage after wet cupping therapy: a case report
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作者 Zainab AlMusleh Ahmad Alrawashdeh Tamer Aboushanab 《Clinical Research Communications》 2024年第2期27-29,共3页
Introduction:Cupping therapy is an integrative and complementary medicine therapy that practiced worldwide by several civilizations for thousands of years.Cupping therapy is reported to be a relatively safe practice e... Introduction:Cupping therapy is an integrative and complementary medicine therapy that practiced worldwide by several civilizations for thousands of years.Cupping therapy is reported to be a relatively safe practice especially if performed by qualified therapists.Subconjunctival hemorrhage(SCH)is a common cause of eye redness and can be classified into traumatic and spontaneous.Case presentation:This case report discussed an adverse event related to wet cupping therapy.The patient developed SCH one day after performing cupping therapy.He was completely recovered after 2 weeks.This case report should increase the knowledge of cupping therapists regarding increasing safety of patient when performing wet cupping therapy on specific body parts especially,head,neck and cervical areas.Furthermore,it highlighted the importance of conducting research regarding the mechanical and physiological effects of cupping therapy.Conclusion:Adverse events related to cupping therapy should be reported with every patient.Cupping therapists should be aware of this possible mild adverse event.To the best of our knowledge,this is the first published adult case of SCH related to wet cupping therapy session.More studies are critically needed for evaluation of cupping therapy effects and adverse events especially when performed on head and neck. 展开更多
关键词 cupping therapy wet cupping Hijama subconjunctival hemorrhage adverse event
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GRINDING FORCE IN PRECISION MACHINING OF WC-Co COATING BY CUP WHEEL 被引量:5
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作者 吴琦 罗玉梅 +2 位作者 刘红斌 胡德金 徐鸿钧 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2007年第2期134-138,共5页
High machining precision and machining efficiency can be obtained in grinding WC-Co coating by cup wheel. The conventional model of the grinding force is not suitable for the grinding of the cup wheel due to the diffe... High machining precision and machining efficiency can be obtained in grinding WC-Co coating by cup wheel. The conventional model of the grinding force is not suitable for the grinding of the cup wheel due to the difference in grinding style between the cup wheel and the conventional external wheel. So the grit grinding process of the cup wheel is studied, and a new concept of the effective grinding width of the cup wheel is presented. Then the grinding force in grinding WC-Co coating materials by cup wheel is analyzed and a theoretical formula is deduced. Finally, experimental results of the grinding force verify the correctness and the precision of the theoretical formula. 展开更多
关键词 cup wheel WC-Co coating grinding force theoretical formula
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影响图及其在Robocup中的应用 被引量:6
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作者 张润梅 王浩 +1 位作者 姚宏亮 方宝富 《系统仿真学报》 EI CAS CSCD 北大核心 2005年第1期134-137,共4页
影响图是决策问题的图形表示。它被认为是对动态贝叶斯网络的扩展,是在贝叶斯网络的基础上增加了决策结点和效用结点。探讨影响图在Robocup中的应用,用分层影响图表示了截球问题的模型,并给出一种实现行为动态调整的算法。仿真结果表明... 影响图是决策问题的图形表示。它被认为是对动态贝叶斯网络的扩展,是在贝叶斯网络的基础上增加了决策结点和效用结点。探讨影响图在Robocup中的应用,用分层影响图表示了截球问题的模型,并给出一种实现行为动态调整的算法。仿真结果表明该算法可行的。 展开更多
关键词 影响图 贝叶斯网络 ROBOcup 多智能体系统
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基于CUPS的网络打印审计技术研究 被引量:5
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作者 张闯 郭燕慧 《软件》 2015年第12期140-145,共6页
随着信息技术的发展,类Unix操作系统的使用越来越广泛。针对目前没有支持多操作系统的打印审计系统的现状,本文提出了一种基于CUPS的打印审计技术。本技术从网络传输层入手,通过CUPS实现了打印数据的拦截,并在此基础实现了身份认证、张... 随着信息技术的发展,类Unix操作系统的使用越来越广泛。针对目前没有支持多操作系统的打印审计系统的现状,本文提出了一种基于CUPS的打印审计技术。本技术从网络传输层入手,通过CUPS实现了打印数据的拦截,并在此基础实现了身份认证、张数限制等打印审计功能。实验结果表明本技术可以在用户不安装附加软件和驱动的前提下实现支持多操作系统的打印审计过程,具有较高的实用价值。 展开更多
关键词 网络打印 审计 cupS UNIX
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酵母耐铜基因CUP1改良荧光假单胞菌耐铜性研究初报 被引量:3
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作者 姜伯乐 吴圣进 何勇强 《中国农学通报》 CSCD 2007年第9期96-99,共4页
利用根际促生菌(PGPR)荧光假单胞菌(Pseudomonas fluorescens)等微生物的生物修复作用可治理土壤重金属污染。酿酒酵母耐铜基因CUP1编码铜金属硫蛋白(Cu-MT),对铜有很高的亲和性,可拮抗重金属铜毒性。为提高荧光假单胞菌的生物修复能力... 利用根际促生菌(PGPR)荧光假单胞菌(Pseudomonas fluorescens)等微生物的生物修复作用可治理土壤重金属污染。酿酒酵母耐铜基因CUP1编码铜金属硫蛋白(Cu-MT),对铜有很高的亲和性,可拮抗重金属铜毒性。为提高荧光假单胞菌的生物修复能力,将酿酒酵母耐铜基因CUP1克隆至荧光假单胞菌。通过引物设计,将SD序列添加至酵母耐铜基因CUP1编码序列前端,PCR扩增得到约200bp的CUP1片段,克隆至测序载体pUC19,经测序证明正确后,胶回收CUP1片段连接到具有广泛宿主范围的柯斯质粒pLAFR3上,经三亲接合转移至荧光假单胞菌AS1.1381,得到重组菌株AS1.1381/pL3CUP1。铜实验表明,重组菌株AS1.1381/pL3CUP1的抗铜能力(6mM Cu2+)显著高于AS1.1381/pLAFR3(4.5mMCu2+),重组菌株抗铜性得到提高。 展开更多
关键词 生物修复 重金属污染 荧光假单胞菌 金属硫蛋白 cup1基因
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pSH-CUP重组质粒的构建及面包酵母酸性海藻糖酶基因的敲除 被引量:4
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作者 和东芹 肖冬光 吕烨 《微生物学报》 CAS CSCD 北大核心 2008年第2期147-151,共5页
设计含有与面包酵母(Saccharomyces cerevisiae BY-6)编码酸性海藻糖酶ATH基因内部部分序列同源的长引物,以质粒pUG6为模板进行PCR构建带有Cre/loxP系统的敲除单元,转化面包酵母获得G418阳性克隆。将铜抗性基因(CUP1-MT1)导入Cre重组酶... 设计含有与面包酵母(Saccharomyces cerevisiae BY-6)编码酸性海藻糖酶ATH基因内部部分序列同源的长引物,以质粒pUG6为模板进行PCR构建带有Cre/loxP系统的敲除单元,转化面包酵母获得G418阳性克隆。将铜抗性基因(CUP1-MT1)导入Cre重组酶表达质粒pSH47,得到重组质粒pSH-CUP,并转化阳性克隆,以铜抗性筛选面包酵母转化子。半乳糖诱导表达Cre酶切除Kan^r基因。重组质粒pSH-CUP的构建,不仅解决了酵母转化子筛选标记问题和非酵母基因的引入,而且使LoxP-kanMX-loxP基因敲除体系在进行真核生物基因敲除时更加方便可行。 展开更多
关键词 面包酵母 基因敲除 铜抗性 pSH-cup重组质粒构建 酸性海藻糖酶
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酿酒酵母耐铜基因CUP1的克隆及其植物表达载体的构建 被引量:1
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作者 何勇强 陶勤南 +2 位作者 唐纪良 臧宁 小畑仁 《广西农业生物科学》 CAS CSCD 2000年第4期233-238,共6页
酿酒酵母铜金属硫蛋白 ( copper metallothionein,Cu MT) ,属于第 II型金属硫蛋白 ,Cu MT由酿酒酵母耐铜基因 CUP1编码 ,由 61个氨基酸残基组成 ,其中半胱氨酸残基 1 3个 ,占 Cu MT总氨基酸数的 2 1 .3%。 Cu MT与铜离子有较强的结合力 ... 酿酒酵母铜金属硫蛋白 ( copper metallothionein,Cu MT) ,属于第 II型金属硫蛋白 ,Cu MT由酿酒酵母耐铜基因 CUP1编码 ,由 61个氨基酸残基组成 ,其中半胱氨酸残基 1 3个 ,占 Cu MT总氨基酸数的 2 1 .3%。 Cu MT与铜离子有较强的结合力 ,在酵母细胞中主要参与过量铜的解毒作用。本研究通过PCR方法 ,从酵母基因组 DNA中扩增出了 CUP1基因 ,克隆于 p UC1 9的多克隆位点。扩增片段长 4 86bp,包括 ORF及其部分下游序列。所得克隆与发表序列的 ORF一致 ,推导出的氨基酸序列与发表序列完全一样。用于植物转 CUP1基因用的表达载体已构建完毕 ,分别命名为 p BI CUP1和 p CM CUP1。对植物 (如苎麻、水稻、水生植物等 ) 展开更多
关键词 植物表达载体 cup1基因 克隆 土壤 重金属污染
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优势关系粗糙集在RoboCup中的决策分析 被引量:3
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作者 李艳 杨习贝 杨静宇 《计算机科学》 CSCD 北大核心 2009年第4期232-234,242,共4页
截球能力是RoboCup(Robot World Cup)仿真比赛中个体智能体的核心技能之一。为提高截球的成功率,将解析法和经验法相结合,提出了一种基于优势关系粗糙集的截球策略。着重讨论了基于优势关系的知识约简和截球决策规则的提取,研究了RoboCu... 截球能力是RoboCup(Robot World Cup)仿真比赛中个体智能体的核心技能之一。为提高截球的成功率,将解析法和经验法相结合,提出了一种基于优势关系粗糙集的截球策略。着重讨论了基于优势关系的知识约简和截球决策规则的提取,研究了RoboCup运动模型中截球点的二分法求解。最后进行了仿真平台中的决策分析,验证了该策略的有效性。 展开更多
关键词 机器人世界杯足球锦标赛 粗糙集 优势关系 截球策略
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LALR(1)解析器的再工程:YACC和CUP剖析 被引量:1
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作者 张昱 张磊 《计算机工程》 EI CAS CSCD 北大核心 2005年第15期50-52,共3页
现有的前端分析多数是通过编写相应的可能含有二义的LALR(1)文法规范,利用YACC或其变种自动生成的。在这些解析器的Java再工程中,可以用CUP去替代YACC,这样再工程的焦点转移到对文法规范的变换。由于YACC及CUP在二义的解决、解析器的构... 现有的前端分析多数是通过编写相应的可能含有二义的LALR(1)文法规范,利用YACC或其变种自动生成的。在这些解析器的Java再工程中,可以用CUP去替代YACC,这样再工程的焦点转移到对文法规范的变换。由于YACC及CUP在二义的解决、解析器的构造等有细微的差别,因此对复杂的文法规范的变换并不是一件容易的事。该文通过剖析YACC和CUP,指出了它们的不同之处,并总结出有二义的YACC文法到CUP文法变换的基本原则和几个变换法则。 展开更多
关键词 LALR(1) 解析器 YACC cup 二义性 冲突
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