A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were wel...A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ? 8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.展开更多
A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liq...A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liquorice (Glycyrrhiza) extracts.The quantitative chemical fingerprint from 50 batches of turmeric and liquorice extracts was established using high performance liquid chromatography hyphenated to an ultraviolet visible detector.Qualitative results were obtained using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.A total of 46 peaks (peaks 1–15 from turmeric and 16–46 from liquorice) were selected as "common peaks" for analysis.The inhibitory effect of the combined extracts on He La cells was measured by MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.It was found that 15 compounds (peaks:8,12,30,24,46,11,14,9,3,1,44,18,7,45 and 43)possessing high absolute MIV exhibited a significant correlation with the cytotoxicity against He La cells; most of these have already been confirmed with potential cytotoxicity in previous research.The important potential application of the present model can be extended to help discover active compounds from complex herbal medicine prior to traditional bioassay-guided separation.It is considered that this could be a useful tool for redeveloping herbal medicine based on the use of these active compounds.展开更多
High blood pressure is the main risk factor for cardiovascular diseases, it affects many people worldwide and is a public health problem. This study explored the acute and chronic actions of a mixture of turmeric (95%...High blood pressure is the main risk factor for cardiovascular diseases, it affects many people worldwide and is a public health problem. This study explored the acute and chronic actions of a mixture of turmeric (95% curcumin) plus black pepper (95% piperine) extracts, in the blood pressure pattern in spontaneously hypertensive rats (SHR) along they age. For the acute study male adult (4 - 7 months old) SHR and their control Wistar Kyoto rats (WKY) were used. A single oral dose of the mixture of turmeric/black pepper extracts (200 mg/2mg, respectively) suspension was administered. Tail-cuff was used to determine blood pressure during 180 min. For the chronic study, young (1-month-old) male SHR and their control WKY rats were fed with standard chow, or standard chow combined with cocoa, or combined with cocoa plus the mixture of turmeric/black pepper extracts;tail-cuff was used to determine blood pressure once a week, along 12 weeks. In a second chronic assay adult (5 months old) male SHR and their control WKY rats were fed with standard chow, or standard chow combined with cocoa, or combined with cocoa plus the mixture of turmeric/black pepper extracts;tail-cuff was used to determine blood pressure once a week, along 12 weeks. In all three studies, a decrease in systolic, diastolic and mean blood pressure was observed, being higher in SHR and negligible in WKY rats. The mixture of turmeric/black pepper extracts showed antihypertensive actions in SHR rats with no effect on WKY rats. The mixture delayed the onset of hypertension in young SHR rats.展开更多
Turmeric (<i>Curcuma</i><span> <i>longa</i> L</span><span><span><span style="font-family:;" "="">.</span></span></span>&...Turmeric (<i>Curcuma</i><span> <i>longa</i> L</span><span><span><span style="font-family:;" "="">.</span></span></span><span><span><span style="font-family:;" "="">) </span></span></span><span><span><span style="font-family:;" "="">is one of the essential condiments all over the world. Turmeric is an important medicine in the eastern medical systems and its therapeutic properties have been well recorded. Curcumin, commonly called diferuloyl methane (hydrophobic polyphenol), was studied extensively for its biological properties. Sri Lankan turmeric records relatively higher percentage (up to 9%) of curcumin. The bioactive compounds with insecticidal or pesticidal activity w</span></span></span><span><span><span style="font-family:;" "="">ere</span></span></span><span><span><span style="font-family:;" "=""> studied from the Sri Lankan turmeric as there is an increasing demand for natural products to control ticks and fleas in dogs against the chemicals that induce some secondary effects. Fifty grams (50 g) of dried coarsely ground turmeric (<i>Curcuma</i><span> <i>longa</i> L</span></span></span></span><span><span><span style="font-family:;" "="">.</span></span></span><span><span><span style="font-family:;" "="">)</span></span></span><span><span><span style="font-family:;" "=""> was subjected to sequential extractions with four different solvents in ascending order of polarity <span>using Soxhlet apparatus.</span></span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">The treatments consisted of solvent-fractionated tur</span></span></span><span><span><span style="font-family:;" "="">meric extracts from hexane, dichloromethane (CH<sub>2</sub>Cl<sub>2</sub>), ethyl acetate (EtOAc) and Methanol (CH<sub>3</sub>OH) dissolved in the water to 100 ppm. The test carried out using a modified olfactometer and the degree of repellency of common tick species <i>Rhipicephalus</i></span></span></span><span><span><i><span style="font-family:;" "=""> </span></i></span></span><span><span><i><span style="font-family:;" "="">sanguineus</span></i></span></span><span><span><span style="font-family:;" "=""> was recorded. The hexane extract from dried turmeric powder showed the highest repellence of 80% whereas other fractions showed lesser repellence. Further studies in the direction of purification, dose dependent repellency and persistency.</span></span></span>展开更多
Objective:To study the chemical constituents from the roots of Curcuma longa.Methods:The structures of the compounds were elucidated based on extensive spectral analysis,including 1D and 2D NMR,MS,UV,and CD analysis.R...Objective:To study the chemical constituents from the roots of Curcuma longa.Methods:The structures of the compounds were elucidated based on extensive spectral analysis,including 1D and 2D NMR,MS,UV,and CD analysis.Results:Two new sesquiterpene compounds(1S,2R,5R,7S,8R)-2,8-epoxy-5-hydroxybisabola-3,10-dioen-9-one(1),(1R,2R,5R,7S,8R)-2,8-epoxy-5-hydroxybisabola-3,10-dioen-9-one(2),and a new natural product 6-(4-Hydroxymethylphenyl)-2-methyl-hept-2-ene-4-one(3)together with three known compounds ar-turmerone(4),2-methyl-6-(4-hydroxyphenyl-3-methyl)-2-hepten-4-one(5)and 2-methyl-6-(4-hydroxyphenyl)-2-hepten-4-one(6)were isolated from C.longa root extract with 95%ethanol.Conclusion:In the study,three new compounds were isolated from C.longa,and their absolute configurations were determined.展开更多
基金supported by the National Scientific Foundation of China (Grant nos.21375101,90817103,and 30973672)Doctroral Fund of Ministry of Education of China (No.20110141110024)Innovation Seed Fund and Translational Medical Research Fund of Wuhan University School of Medicine
文摘A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ? 8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.
基金financial support provided by the National Natural Science Foundation of China(No.81102900)
文摘A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liquorice (Glycyrrhiza) extracts.The quantitative chemical fingerprint from 50 batches of turmeric and liquorice extracts was established using high performance liquid chromatography hyphenated to an ultraviolet visible detector.Qualitative results were obtained using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.A total of 46 peaks (peaks 1–15 from turmeric and 16–46 from liquorice) were selected as "common peaks" for analysis.The inhibitory effect of the combined extracts on He La cells was measured by MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.It was found that 15 compounds (peaks:8,12,30,24,46,11,14,9,3,1,44,18,7,45 and 43)possessing high absolute MIV exhibited a significant correlation with the cytotoxicity against He La cells; most of these have already been confirmed with potential cytotoxicity in previous research.The important potential application of the present model can be extended to help discover active compounds from complex herbal medicine prior to traditional bioassay-guided separation.It is considered that this could be a useful tool for redeveloping herbal medicine based on the use of these active compounds.
文摘High blood pressure is the main risk factor for cardiovascular diseases, it affects many people worldwide and is a public health problem. This study explored the acute and chronic actions of a mixture of turmeric (95% curcumin) plus black pepper (95% piperine) extracts, in the blood pressure pattern in spontaneously hypertensive rats (SHR) along they age. For the acute study male adult (4 - 7 months old) SHR and their control Wistar Kyoto rats (WKY) were used. A single oral dose of the mixture of turmeric/black pepper extracts (200 mg/2mg, respectively) suspension was administered. Tail-cuff was used to determine blood pressure during 180 min. For the chronic study, young (1-month-old) male SHR and their control WKY rats were fed with standard chow, or standard chow combined with cocoa, or combined with cocoa plus the mixture of turmeric/black pepper extracts;tail-cuff was used to determine blood pressure once a week, along 12 weeks. In a second chronic assay adult (5 months old) male SHR and their control WKY rats were fed with standard chow, or standard chow combined with cocoa, or combined with cocoa plus the mixture of turmeric/black pepper extracts;tail-cuff was used to determine blood pressure once a week, along 12 weeks. In all three studies, a decrease in systolic, diastolic and mean blood pressure was observed, being higher in SHR and negligible in WKY rats. The mixture of turmeric/black pepper extracts showed antihypertensive actions in SHR rats with no effect on WKY rats. The mixture delayed the onset of hypertension in young SHR rats.
文摘Turmeric (<i>Curcuma</i><span> <i>longa</i> L</span><span><span><span style="font-family:;" "="">.</span></span></span><span><span><span style="font-family:;" "="">) </span></span></span><span><span><span style="font-family:;" "="">is one of the essential condiments all over the world. Turmeric is an important medicine in the eastern medical systems and its therapeutic properties have been well recorded. Curcumin, commonly called diferuloyl methane (hydrophobic polyphenol), was studied extensively for its biological properties. Sri Lankan turmeric records relatively higher percentage (up to 9%) of curcumin. The bioactive compounds with insecticidal or pesticidal activity w</span></span></span><span><span><span style="font-family:;" "="">ere</span></span></span><span><span><span style="font-family:;" "=""> studied from the Sri Lankan turmeric as there is an increasing demand for natural products to control ticks and fleas in dogs against the chemicals that induce some secondary effects. Fifty grams (50 g) of dried coarsely ground turmeric (<i>Curcuma</i><span> <i>longa</i> L</span></span></span></span><span><span><span style="font-family:;" "="">.</span></span></span><span><span><span style="font-family:;" "="">)</span></span></span><span><span><span style="font-family:;" "=""> was subjected to sequential extractions with four different solvents in ascending order of polarity <span>using Soxhlet apparatus.</span></span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">The treatments consisted of solvent-fractionated tur</span></span></span><span><span><span style="font-family:;" "="">meric extracts from hexane, dichloromethane (CH<sub>2</sub>Cl<sub>2</sub>), ethyl acetate (EtOAc) and Methanol (CH<sub>3</sub>OH) dissolved in the water to 100 ppm. The test carried out using a modified olfactometer and the degree of repellency of common tick species <i>Rhipicephalus</i></span></span></span><span><span><i><span style="font-family:;" "=""> </span></i></span></span><span><span><i><span style="font-family:;" "="">sanguineus</span></i></span></span><span><span><span style="font-family:;" "=""> was recorded. The hexane extract from dried turmeric powder showed the highest repellence of 80% whereas other fractions showed lesser repellence. Further studies in the direction of purification, dose dependent repellency and persistency.</span></span></span>
基金Sub-project of National Basic Science Talent Training Fund(No.J1103606)。
文摘Objective:To study the chemical constituents from the roots of Curcuma longa.Methods:The structures of the compounds were elucidated based on extensive spectral analysis,including 1D and 2D NMR,MS,UV,and CD analysis.Results:Two new sesquiterpene compounds(1S,2R,5R,7S,8R)-2,8-epoxy-5-hydroxybisabola-3,10-dioen-9-one(1),(1R,2R,5R,7S,8R)-2,8-epoxy-5-hydroxybisabola-3,10-dioen-9-one(2),and a new natural product 6-(4-Hydroxymethylphenyl)-2-methyl-hept-2-ene-4-one(3)together with three known compounds ar-turmerone(4),2-methyl-6-(4-hydroxyphenyl-3-methyl)-2-hepten-4-one(5)and 2-methyl-6-(4-hydroxyphenyl)-2-hepten-4-one(6)were isolated from C.longa root extract with 95%ethanol.Conclusion:In the study,three new compounds were isolated from C.longa,and their absolute configurations were determined.