Precipitates Generation Mechanism and Surface Quality Improvement for Aluminum Alloy 6061 in Diamond Cutting

To improve the surface quality for aluminum alloy 6061(Al6061) in ultra-precision machining, we investigated the factors affecting the surface finish in single point diamond turning(SPDT)by studying influence of the precipitates generation of Al6061 on surface integrity and surface roughness.Based on the Johnson-Mehl-Avrami solid phase transformation kinetics equation, theoretical and experimental studies were conducted to build the relationship between the aging condition and the type, size and number of the precipitates for Al6061. Diamond cutting experiments were conducted to machine Al6061 samples under different aging conditions. The experimental results show that, the protruding on the chip surface is mainly Mg_(2)Si and the scratches on the machined surface mostly come from the iron-containing phase(α-, β-AlFeSi).Moreover, the generated Mg_(2)Si and α-, β-AlFeSi affect the surface integrity and the diamond turned surface roughness. Especially, the achieved surface roughness in SPDT is consistent with the variation of the number of AlFeSi and Mg_(2)Si with the medium size(more than 1 μm and less than 2 μm) in Al6061.

Instability mechanism of mining roadway passing through fault at different angles in kilometre-deep mine and control measures of roof cutting and NPR cables

The angle α between the fault strike and the axial direction of the roadway produces different damage characteristics. In this paper, the research methodology includes theoretical analyses, numerical simulations and field experiments in the context of the Daqiang coal mine located in Shenyang, China. The stability control countermeasure of "pre-splitting cutting roof + NPR anchor cable"(PSCR-NPR) is simultaneously proposed. According to the different deformation characteristics of the roadway, the faults are innovatively classified into three types, with α of type I being 0°-30°, α of type II being 30°-60°, and α of type III being 60°-90°. The full-cycle stress evolution paths during mining roadway traverses across different types of faults are investigated by numerical simulation. Different pinch angles α lead to high stress concentration areas at different locations in the surrounding rock. The non-uniform stress field formed in the shallow surrounding rock is an important reason for the instability of the roadway. The pre-cracked cut top shifted the high stress region to the deep rock mass and formed a low stress region in the shallow rock mass. The high prestressing NPR anchor cable transforms the non-uniform stress field of the shallow surrounding rock into a uniform stress field. PSCR-NPR is applied in the fault-through roadway of Daqiang mine. The low stress area of the surrounding rock was enlarged by 3-7 times, and the cumulative convergence was reduced by 45%-50%. It provides a reference for the stability control of the deep fault-through mining roadway.

Endoscopic radial incision and cutting method for adult congenital duodenal webs:A case report

BACKGROUND Congenital duodenal webs are rare in adults and can lead to various symptoms such as nausea,vomiting,and postprandial fullness.The treatment for this disease is mostly surgical.Endoscopic treatment techniques have been developed and attempted for this disease.Endoscopic radial incision and cutting(RIC)techniques are reportedly very effective in benign anastomotic stricture.This case report highlights the effectiveness and safety of endoscopic RIC as a minimally invasive treatment for adult congenital duodenal webs.CASE SUMMARY A 23-year-old female patient with indigestion was referred to a tertiary hospital.The patient complained of postprandial fullness in the epigastric region.Previous physical examinations or blood tests indicated no abnormalities.Computed tomography revealed an eccentric broad-based delayed-enhancing mass-like lesion in the second portion of the duodenum.Endoscopy showed an enlarged gastric cavity and a significantly dilated duodenal bulb;a very small hole was observed in the distal part of the second portion,and scope passage was not possible.Gastrografin upper gastrointestinal series was performed,revealing an intraduodenal barium contrast-filled sac with a curvilinear narrow radiolucent rim,a typical"windsock"sign.Endoscopic RIC was performed on the duodenal web.The patient recovered uneventfully.Follow-up endoscopy showed a patent duodenal lumen without any residual stenosis.The patient reported complete resolution of symptoms at the 18-month follow-up.CONCLUSION Endoscopic RIC may be an effective treatment for congenital duodenal webs in adults.

ONECUT2高表达介导胃癌复发患者5-FU化疗耐药

目的:探究One cut结构域家族成员2(ONECUT2)是否可以作为对5-FU耐药的胃癌复发患者的治疗靶点。方法:利用癌症基因组图谱(TCGA)数据库分析ONECUT2基因在胃癌患者及胃癌复发患者的表达情况。利用基因集富集分析(GSEA)评估TCGA数据库中ONECUT2表达量与药物代谢相关生物学过程的相关性。利用CCK-8细胞毒性实验评估胃癌细胞敲低或过表达ONECUT2对5-FU的耐药情况。分析30例胃癌术后复发患者ONECUT2表达量与5-FU治疗效果的相关性。结果:TCGA数据库分析显示,在经受过化疗且复发的胃癌患者中,ONECUT2高表达的患者无病间隔期(disease-free interval,DFI)更短。GSEA分析结果表明,ONECUT2高表达与异生药物代谢通路呈正相关,这提示可能与胃癌患者的耐药相关。体外实验中,敲低ONECUT2降低胃癌细胞对5-FU的半数抑制浓度(IC_(50))值;反之,过表达ONECUT2增加细胞对5-FU的IC_(50)值。30例胃癌复发患者对5-FU的药物敏感性与ONECUT2表达量相关。结论:胃癌复发患者的ONECUT2表达量更高,ONECUT2高表达与胃癌复发患者的5-FU耐药相关,可能是潜在的治疗靶点。

Stability control measures for roof cutting and NPR supporting of mining roadways in fault areas of kilometre-deep coal mine

The study focuses on the stability control measures for mining roadways in fault zones of deep mines,using Daqiang Coal Mine as a case study.The control system under consideration,referred to as"pre-splitting cutting roof+NPR anchor cable"(PSCR-NPR),is subjected to scrutiny through theoretical analysis,numerical modelling,and field trials.Furthermore,a comprehensive analysis is undertaken to evaluate the stability control mechanism of this particular technology.The study provides evidence that the utilization of deep-hole directional energy-concentrated blasting facilitates the attainment of directional roof cutting in roadways.The aforementioned procedure leads to the formation of a uniform structural surface on the roof of the roadway and causes modifications in the surrounding geological formation.The examination of the lateral abutment pressure and shear stress distribution,both prior to and subsequent to roof cutting,indicates that the implementation of pre-splitting techniques leads to a noteworthy reduction in pressure.The proposition of incorporating the safety factor Q for roof cutting height is suggested as a method to augment comprehension of the pressure relief phenomenon in the field of engineering.The analysis of numerical simulation has indicated that the optimal pressure relief effect of a mining roadway in a fault area is attained when the value of Q is 1.8.The NPR anchor cable exhibits noteworthy characteristics,including a high level of prestress,continuous resistance,and substantial deformation.After the excavation of the roadway,a notable reduction in radial stress occurs,leading to the reinstatement of the three-phase stress state in the surrounding rock.This restoration is attributed to the substantial prestress exerted on the radial stress.The termination point of the NPR anchor cable is strategically positioned within a stable rock formation,allowing for the utilization of the mechanical characteristics of the deep stable rock mass.This positioning serves to improve the load-bearing capacity of the surrounding rock.The mining roadway within the fault region of Daqiang Coal Mine is outfitted with the PSCR-NPR technology.The drop in shear stress experienced by the rock surrounding the roadway is estimated to be around 30%,whilst the low-stress region of the mining roadway extends by a factor of approximately 5.5.The magnitude of surface displacement convergence experiences a decrease of approximately 45%-50%.The study’s findings provide useful insights regarding the stable of mining roadway in characterized by fault zones.

Performances of a Stinger PDC cutter breaking granite: Cutting force and mechanical specific energy in single cutter tests

The Stinger PDC cutter has high rock-breaking efficiency and excellent impact and wear resistance, which can significantly increase the rate of penetration (ROP) and extend PDC bit life for drilling hard and abrasive formation. The knowledge of force response and mechanical specific energy (MSE) for the Stinger PDC cutter is of great importance for improving the cutter's performance and optimizing the hybrid PDC bit design. In this paper, 87 single cutter tests were conducted on the granite. A new method for precisely obtaining the rock broken volume was proposed. The influences of cutting depth, cutting angle, and cutting speed on cutting force and MSE were analyzed. Besides, a phenomenological cutting force model of the Stinger PDC cutter was established by regression of experimental data. Moreover, the surface topography and fracture morphology of the cutting groove and large size cuttings were measured by a 3D profilometer and a scanning electron microscope (SEM). Finally, the rock-breaking mechanism of the Stinger PDC cutter was illustrated. The results indicated that the cutting depth has the greatest influence on the cutting force and MSE, while the cutting speed has no obvious effects, especially at low cutting speeds. As the increase of cutting depth, the cutting force increases linearly, and MSE reduces with a quadratic polynomial relationship. When the cutting angle raises from 10° to 30°, the cutting force increases linearly, and the MSE firstly decreases and then increases. The optimal cutting angle for breaking rock is approximately 20°. The Stinger PDC cutter breaks granite mainly by high concentrated point loading and tensile failure, which can observably improve the rock breaking efficiency. The key findings of this work will help to reveal the rock-breaking mechanisms and optimize the cutter arrangement for the Stinger PDC cutter.

Numerical simulation of materials-oriented ultra-precision diamond cutting:review and outlook

Ultra-precision diamond cutting is a promising machining technique for realizing ultra-smooth surface of different kinds of materials.While fundamental understanding of the impact of workpiece material properties on cutting mechanisms is crucial for promoting the capability of the machining technique,numerical simulation methods at different length and time scales act as important supplements to experimental investigations.In this work,we present a compact review on recent advancements in the numerical simulations of material-oriented diamond cutting,in which representative machining phenomena are systematically summarized and discussed by multiscale simulations such as molecular dynamics simulation and finite element simulation:the anisotropy cutting behavior of polycrystalline material,the thermo-mechanical coupling tool-chip friction states,the synergetic cutting responses of individual phase in composite materials,and the impact of various external energetic fields on cutting processes.In particular,the novel physics-based numerical models,which involve the high precision constitutive law associated with heterogeneous deformation behavior,the thermo-mechanical coupling algorithm associated with tool-chip friction,the configurations of individual phases in line with real microstructural characteristics of composite materials,and the integration of external energetic fields into cutting models,are highlighted.Finally,insights into the future development of advanced numerical simulation techniques for diamond cutting of advanced structured materials are also provided.The aspects reported in this review present guidelines for the numerical simulations of ultra-precision mechanical machining responses for a variety of materials.

Friction behaviors in the metal cutting process:state of the art and future perspectives

Material removal in the cutting process is regarded as a friction system with multiple input and output variables.The complexity of the cutting friction system is caused by the extreme conditions existing on the tool–chip and tool–workpiece interfaces.The critical issue is significant to use knowledge of cutting friction behaviors to guide researchers and industrial manufacturing engineers in designing rational cutting processes to reduce tool wear and improve surface quality.This review focuses on the state of the art of research on friction behaviors in cutting procedures as well as future perspectives.First,the cutting friction phenomena under extreme conditions,such as high temperature,large strain/strain rates,sticking–sliding contact states,and diverse cutting conditions are analyzed.Second,the theoretical models of cutting friction behaviors and the application of simulation technology are discussed.Third,the factors that affect friction behaviors are analyzed,including material matching,cutting parameters,lubrication/cooling conditions,micro/nano surface textures,and tool coatings.Then,the consequences of the cutting friction phenomena,including tool wear patterns,tool life,chip formation,and the machined surface are analyzed.Finally,the research limitations and future work for cutting friction behaviors are discussed.This review contributes to the understanding of cutting friction behaviors and the development of high-quality cutting technology.

Experimental research and application of cold cutting to hot stamping parts based on multiphase microstructure

Among the bottlenecks that hinder the improvement of the production efficiency of hot stamping are high strength and difficulty in edge cutting and hole punching.Starting from the preparation of hot stamping multiphase microstructure materials,this paper developed a plate quenching die system with controllable surface temperature and prepared four types of hot stamping plates with different martensite volume fractions.Then,straight edge cold cutting experiments were performed to study the influence of cutting clearance and cutting force on fracture quality.The results show that the bright zone is the largest when the cutting clearance is 0.14 mm,and the cutting experience coefficient of the hot stamping sheet with each martensite volume fraction is obtained when the cutting clearance is 0.14 mm.The research results of this paper were applied to the production of hot stamping parts.

Experimental investigation on the cuttings formation process and its relationship with cutting force in single PDC cutter tests

The single polycrystalline diamond compact(PDC)cutter test is widely used to investigate the mecha-nism of rock-breaking.The generated cuttings and cutting force are important indexes reflecting the rock failure process.However,they were treated as two separate parameters in previous publications.In this study,through a series of rock block cutting tests,the relationship between them was investigated to obtain an in-depth understanding of the formation of cuttings.In addition,to validate the standpoints obtained in the aforementioned experiments,rock sheet cutting tests were conducted and the rock failure process was monitored by a high-speed camera frame by frame.The cutting force was recorded with the same sampling rate as the camera.By this design,every sampled point of cutting force can match a picture captured by the camera,which reflects the interaction between the rock and the cutter.The results indicate that the increase in cutting depth results in a transition of rock failure modes.At shallow cutting depth,ductile failure dominates and all the cuttings are produced by the compression of the cutter.The corresponding cutting force fluctuates slightly.However,beyond the critical depth,brittle failure occurs and chunk-like cuttings appear,which leads to a sharp decrease in cutting force.After that,the generation of new surface results in a significant decrease in actual cutting depth,a parameter proposed to reflect the interaction between the rock and the cutter.Consequently,ductile failure dominates again and a slight fluctuation of cutting force can be detected.As the cutter moves to the rock,the actual cutting depth gradually increases,which results in the subsequent generation of chunk-like cuttings.It is accompanied by an obvious cutting force drop.That is,ductile failure and brittle failure,one following another,present at large cutting depth.The transition of rock failure mode can be correlated with the variation of cutting force.Based on the results of this paper,the real-time monitoring of torque may be helpful to determine the efficiency of PDc bits in the downhole.

Effects of carcass weight, sex and breed composition on meat cuts and carcass trait in finishing pigs

Pork cutting is a very important processing in promoting economic appreciation across the swine business chain. The goal of this research is to determine the proportion and weight of meat cuts, as well as to analyze the effects of carcass weight, sex and breed composition on meat cuts. Simultaneously, we investigate the correlation between meat cuts, carcass traits and meat quality traits. To assess 17 meat cut traits, 12 carcass traits and 6 meat quality traits, we sample 2 012 pigs from four breeds, including Landrace(LD), Yorkshire(YK), Landrace Yorkshire(LY), and Duroc Landrace Yorkshire(DLY). The results showed that carcass weight, sex and breed composition have significant effects on the weight and proportion of most meat cuts. The proportion of cuts for muscle and bone decrease as carcass weight grows, whereas the proportion of cuts for fat increases. Moreover, the thickness of four-point backfat was significantly increasing(P<0.001) with increase of carcass weights, indicating that large amount of intaking energy in the late finishing stage was used for fat deposition. Besides, the proportion of Shoulder cut(SC) and Back fat(BF) in barrows was significantly higher(P<0.001) than that in sows, whereas the Leg cut(LC) showed the opposite trend. The Loin(LO) proportion and Loin muscle area(LMA) of barrows were significantly lower(P<0.001), but the proportion of fat areas in the image(PFAI) and visual marbling score(VMS) were significantly higher(P<0.001) than those of sows, respectively. In terms of breeds, LD had the longest straight carcass length, significantly longer(P<0.001) than the other three breeds, which partially explains why LD had the largest proportion of the Middle cut(MC). Moreover, the proportion of SC in DLY was the highest. Last but not least, the correlations between the proportions of most meat cuts, and also between meat cuts and meat quality or carcass traits were low or not significant(P>0.05). The effects of carcass weight, sex and breed composition on the meat cuts, meat quality and carcass traits are breed and growth stage dependent. It also reflects the asynchrony of the growth curve between different sexes. Our results laid an important foundation for breeding pig carcass cuts and composition.

Genetic dissection and genomic prediction for pork cuts and carcass morphology traits in pig

Background As pre-cut and pre-packaged chilled meat becomes increasingly popular,integrating the carcasscutting process into the pig industry chain has become a trend.Identifying quantitative trait loci(QTLs)of pork cuts would facilitate the selection of pigs with a higher overall value.However,previous studies solely focused on evaluating the phenotypic and genetic parameters of pork cuts,neglecting the investigation of QTLs influencing these traits.This study involved 17 pork cuts and 12 morphology traits from 2,012 pigs across four populations genotyped using CC1 PorcineSNP50 BeadChips.Our aim was to identify QTLs and evaluate the accuracy of genomic estimated breed values(GEBVs)for pork cuts.Results We identified 14 QTLs and 112 QTLs for 17 pork cuts by GWAS using haplotype and imputation genotypes,respectively.Specifically,we found that HMGA1,VRTN and BMP2 were associated with body length and weight.Subsequent analysis revealed that HMGA1 primarily affects the size of fore leg bones,VRTN primarily affects the number of vertebrates,and BMP2 primarily affects the length of vertebrae and the size of hind leg bones.The prediction accuracy was defined as the correlation between the adjusted phenotype and GEBVs in the validation population,divided by the square root of the trait’s heritability.The prediction accuracy of GEBVs for pork cuts varied from 0.342 to 0.693.Notably,ribs,boneless picnic shoulder,tenderloin,hind leg bones,and scapula bones exhibited prediction accuracies exceeding 0.600.Employing better models,increasing marker density through genotype imputation,and pre-selecting markers significantly improved the prediction accuracy of GEBVs.Conclusions We performed the first study to dissect the genetic mechanism of pork cuts and identified a large number of significant QTLs and potential candidate genes.These findings carry significant implications for the breeding of pork cuts through marker-assisted and genomic selection.Additionally,we have constructed the first reference populations for genomic selection of pork cuts in pigs.

Cutting Force Fluctuation Suppression and Error Homogenization of Noncircular Gear Hobbing Based on the Tool Shifting Method

The current research on noncircular hobbing mainly focuses on the linkage model and motion realization.However,the intermittent cutting characteristics of hobbing would increase uncertainties in the manufacturing process.In this paper,a hobbing machining model with tool-shifting characteristics was proposed to solve the problems of cutting force fluctuation and inconsistency of tooth profile envelope accuracy at different positions of the pitch curve in noncircular gear hobbing.Based on the unit cutting force coefficient method,the undeformed chip volume generated by interrupted cutting was used to characterize the fluctuation trend of the hobbing force.The fluctuation characteristics of the cutting force generated by different hobbing models were compared and analyzed.Using the equivalent gear tooth and hob slotting numbers,an analysis model of the tooth profile envelope error of the noncircular gear was constructed.Subsequently,the tooth profile envelope errors at different positions of the pitch curve were compared and analyzed based on the constructed model.The transmission structure of the electronic gearbox was constructed based on the proposed hobbing model,and the hobbing experiment was conducted based on the selfdeveloped noncircular gear CNC hobbing system.This paper proposes a hobbing method that can effectively suppress the fluctuation of the peak and whole circumference cutting force and reduce the maximum envelope error of the whole circumference gear teeth.

Theoretical and numerical simulation investigation of deep hole dispersed charge cut blasting

Drilling and blasting methods have been used as a common driving technique for shallow-hole driving and blasting in rock roadways.With the advent of digital electronic detonators and the need for increased production efciency,the traditional blasting design is no longer suitable for deep hole blasting.In this paper,a disperse charge cut blasting method was proposed to address the issues of low excavation depth and high block rate in deep hole undercut blasting.First,a blasting model was used to illustrate the mechanism of the deep hole dispersive charge cut blasting process.Then,continuous charge and dispersed charge blasting models were developed using the smooth particle hydrodynamics-fnite element method(SPHFEM).The cutting parameters were determined theoretically,and the cutting efciency was introduced to evaluate the cutting efect.The blasting efects of the two charging models were analyzed utilizing the evolution law of rock damage,the number of rock particles thrown,and the cutting efciency.The results show that using a dispersed charge improves the cutting efciency by about 20%and the rock breakage for the deep hole cut blasting compared to the traditional continuous charge.In addition,important parameters such as cutting hole spacing,cutting hole depth and upper charge proportion also have a signifcant impact on the cutting efect.Finally,the deep hole dispersed charge cut blasting technology is combined with the digital electronic detonator through the feld engineering practice.It provides a reference for the subsequent deep hole cutting blasting and the use of electronic detonators in rock roadways.

Microstructure Transformation and Refinement Mechanism of Undercooled Cu-Ni-Co Alloy Based on Simulation of Critical Cutting Speed in Ultrasonic Machining

Both Cu60Ni38Co2 and Cu60Ni40 alloy were naturally cooled after rapid solidification from the liquid phase.The transformation law of the microstructure characteristics of the rapidly solidified alloy with the change of undercooling(ΔT)was systematically studied.It is found that the two alloys experience the same transformation process.The refinement structures under different undercoolings were characterized by electron backscatter diffraction(EBSD).The results show that the characteristics of the refinement structure of the two alloys with low undercooling are the same,but the characteristics of the refinement structure with high undercooling are opposite.The transmission electron microscopy(TEM)results of Cu60Ni38Co2 alloy show that the dislocation network density of low undercooled microstructure is lower than that of high undercooled microstructure.By combining EBSD and TEM,it could be confirmed that the dendrite remelting fracture is the reason for the refinement of the low undercooled structure,while the high undercooled structure is refined due to recrystallization.On this basis,in the processing of copper base alloys,there will be serious work hardening phenomenon and machining hard problem of consciousness problems caused by excessive cutting force.A twodimensional orthogonal turning finite element model was established using ABAQUS software to analyze the changes in cutting speed and tool trajectory in copper based alloy ultrasonic elliptical vibration turning.The results show that in copper based alloy ultrasonic elliptical vibration turning,cutting process parameters have a significant impact on cutting force.Choosing reasonable process parameters can effectively reduce cutting force and improve machining quality.

Hydraulic modeling and optimization of jet mill bit considering the characteristics of depressurization and cuttings cleaning

A jet mill bit(JMB)is proposed to increase the drilling efficiency and safety of horizontal wells,which has the hydraulic characteristics of depressurization and cuttings cleaning.This paper fills the gap in the hydraulic study of the JMB by focusing on the hydraulic modeling and optimization of the JMB and considering these two hydraulic characteristics.First,the hydraulic depressurization model and the hydraulic cuttings cleaning model of the JMB are developed respectively.In the models,the pressure ratio and efficiency are chosen as the evaluation parameters of the depressurization capacity of the JMB,and the jet hydraulic power and jet impact force are chosen as the evaluation parameters of cuttings cleaning capacity of the JMB.Second,based on the hydraulic models,the effects of model parameters[friction loss coefficient,target inclination angle,rate of penetration(ROP),flow ratio,and well depth]on the hydraulic performance of the JMB are investigated.The results show that an increase in the friction loss coefficient and target inclination angle cause a significant reduction in the hydraulic depressurization capacity,and the effect of ROP is negligible.The flow ratio is positively related to the hydraulic cuttings cleaning capacity,and the well depth determines the maximum hydraulic cuttings cleaning capacity.Finally,by combining the hydraulic depressurization model and hydraulic cuttings cleaning model,an optimization method of JMB hydraulics is proposed to simultaneously maximize the jet depressurization capacity and the cuttings cleaning capacity.According to the drilling parameters given,the optimal values of the drilling fluid flow rate,backward nozzle diameter,forward nozzle diameter,and throat diameter can be determined.Moreover,a case study is conducted to verify the effectiveness of the optimization method.

Evaluation of the treatment effect of rear slope cutting on hydrodynamic pressure landslides:A case study

After the impoundment of the Three Gorges Reservoir,some huge ancient landslides were reactivated and deformed,showing typical hydrodynamic pressure landslide characteristics.The Baishuihe landslide was a typical hydrodynamic pressure landslide.The management department conducted slope cutting treatments from 2018 to 2019.To evaluate the treatment effect of rear slope cutting,this study analyzed the data of the surface deformation survey and field monitoring over the past 20 years and the characteristics of the reservoir water-triggered Baishuihe landslide deformation,and calculated the seepage field,displacement field,and stability coefficient before and after landslide treatment.The results showed that the deformation of the Baishuihe landslide was primarily related to a decrease in the reservoir water level.Owing to the poor permeability of the landslide soil,the decrease in the reservoir water level produced a seepage force pointing to the outside of the landslide body,leading to the step deformation of the landslide displacement.The landslide was treated by rear slope cutting,and the“step”deformation of the landslide disappeared after treatment.The hydrodynamic pressure caused by the change in reservoir water after cutting the slope did not disappear.However,as the slope cutting greatly reduced the overall sliding force of the landslide,its stability was greatly improved.Notably,high stability can still be ensured under extreme rainfall after treatment.Slope cutting is effective for treating hydrodynamic pressure landslides.This study can provide effective technical support for the treatment of reservoir landslides.

Effects of Root Cutting on Morphological Characteristics and Endogenous Hormone Levels of Quercus variabilis Seedlings

The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots,and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term.The morphological characteristics and endogenous hormone contents of normal roots(no root cutting,CK)and cut roots(cut by 1/3 of the length of the main root,RP)were determined by liquid chromatography,which was combined with mass spectrometry at different levels of different developmental stages.The results showed that the root growth indexes and root endogenous hormones in the RP group were superior to those in the CK group.Through comprehensive analysis of endogenous hormones,it was found that the crosstalk of IAA,JA,ABA and SA could activate the root growth defense.After the root cutting treatment,the root growth of Quercus variabilis seedlings could compensate for the inhibition of taproot growth by promoting lateral root growth.The growth and development of compensatory lateral roots contribute to the increase the total root length of plants,thus promoting the absorption of water and nutrients.It is speculated that plant hormones may be the key factors affecting their development,but this is not only related to the content of a single hormone,but more importantly,it is the interaction between various hormones.

Analysis of Friction and Heat Transfer Characteristics of Tubes with Trapezoidal Cut Twisted Tape Inserts

The thermo-hydraulic properties of circular tubes with a twisted tape inside(used accordingly to induce turbulence and enhance heat transfer through the tube wall)are described for Reynolds Numbers ranging from 830 to 1990.Tapes twisted with the three distinct twist ratios are considered,namely,6,4.4 and 3.Air is used as the working fluid in several tests.For the sake of comparison,the standard tube with no insert is also examined.It is shown that in the presence of the twisted tape,the‘frictional factor’,‘Nusselt Number’and the‘thermal performance factor’are much higher than those obtained for the plain tube.Moreover,the tapes having the lowest twist ratio,i.e.,3,are more effective than the others in terms of heat transfer augmentation.The‘thermal performance factor’is greater than one for all the twisted tapes used in the experiments,which confirms the enhanced performances of the heat exchanger and the related savings in terms of total energy.

Physiological Dynamics and Transcriptomic Analysis of Cut Roses‘Carola’Treated with KNO_(3)

The consumption of cut roses(Rosa hybrida)has always ranked first in the world.However,it is vulnerable to rapid petal and leaf wilting due to leaf stomatal water loss,which seriously affects its ornamental quality and economic value.Stomatal movement,a key in plant physiological processes,is influenced by potassium and nitrate.Advancing comprehension of its physiological and molecular mechanism holds promise for preserving the freshness of cut roses.This study observed the impacts of different concentrations of KNO_(3) vase treatments on stomatal opening and water loss in cut rose‘Carola’leaves,as well as their transcriptional responses to KNO_(3).Water loss rates were influenced by KNO_(3) concentrations,with the 25 and 75 mmol/L treatments exhibiting the highest water loss rates.The stomatal aperture reached its widest value when treated with 75 mmol/L KNO_(3).Transcriptional sequencing analysis was performed to identify differentially expressed genes(DEGs)of which 5456 were up-regulated,and 6607 were down-regulated associated with photosynthesis,starch and sucrose metabolism,metabolic pathways,plant-pathogen interaction,plant hormone signal transduction,and related pathways.246 DEGs were selected related to response to KNO_(3) treatment,of which gene ontology(GO)enrichment were nitrate and terpenoid metabolism,ion transport,and response to stimuli.Further heatmap analysis revealed that several genes related to nitrate transport a metabolism,K+transport,vacuoles,and aquaporin were in close association with the response to KNO_(3) treatment.Weighted gene co-expression network analysis(WGCNA)revealed that hub genes,including LAX2,TSJT1,and SCPL34 were identified in turquoise,black,and darkgreen module.Transcription factors such as NAC021,CDF3,ERF053,ETR2,and ARF6 exhibited regulatory roles in the response to KNO_(3) treatment under light conditions.These findings provide valuable insights into the physiological and molecular mechanisms underlying the response of cut rose leaves to KNO_(3) treatment.