Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ（PSⅡ...Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ（PSⅡ） in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited.We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp.PCC 6803. Here, we report that Slr0151 is involved in PSⅡ repair of the organism. We generated a mutant strain（Dslr0151）lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSⅡ activity in Synechocystis. Moreover,the mutant exhibited increased photoinhibition due to impairment of PSⅡ repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSⅡ repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSⅡ in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together,these results indicate that Slr0151 plays an important role in regulating PSⅡ repair in the organism under high-light stress condition.展开更多
基金supported by the National Natural Science Foundation of China (31000018 and 30770037)the Ministry of Science and Technology of China (2009CB220000)+1 种基金the Chinese Academy of Sciencesby grant (M408030) from NTU, Singapore (to B. N.)
文摘Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ（PSⅡ） in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited.We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp.PCC 6803. Here, we report that Slr0151 is involved in PSⅡ repair of the organism. We generated a mutant strain（Dslr0151）lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSⅡ activity in Synechocystis. Moreover,the mutant exhibited increased photoinhibition due to impairment of PSⅡ repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSⅡ repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSⅡ in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together,these results indicate that Slr0151 plays an important role in regulating PSⅡ repair in the organism under high-light stress condition.
