Effect of Different Size of Ground Cyanotis arachnoidea Particles on the Release of β-ecdysone

[Objective] The aim was to investigate the influence of different size of ground Cyanotis arachnoidea C.B.Clarke particles on the release of β-ecdysone.[Method] The content of β-ecdysone extracted from Cyanotis arachnoidea powders of different sizes was detected through HPLC,under the chromatographic conditions：GRACE ODS C18 column（250 mm × 4.6 mm,5 μm）,mobile phase methanol：water= 40：60（V/V）,column temperature 26 ℃,flow rate 1.0 ml/min and detection wavelength 248 nm.[Result] The extracted β-ecdysone from ultra-fine Cyanotis arachnoidea powder（particle size 10,30 and 50 μm） was as more than two folds as common powder（particle size 180 μm）.[Conclusion] The β-ecdysone in ultra-fine powder of Cyanotis arachnoidea was more easily soluble in methanol.The extraction rate of β-ecdysone was significantly higher than that of common powder.The result showed that the technology of ultra-fine pulverization markedly improved the dissolution of the β-ecdysone for increasing the cell breakage rate,and then the active ingredients could be extracted more easily.

A New Phytosterone from Cyanotis arachnoidea

A new phytosterone named cyanosterone B (1) has been isolated from Cyanotis arachnoidea C. B. Clarke. The structure of cyanosterone B was elucidated as 3 b, 5 b, 14 a, 20 R, 22 R-pentahydroxy-cholest-7-en-6-one on the basis of spectroscopic analysis.

露水草加工粒径和其β-蜕皮激素释放的关系

[目的]比较不同粒径露水草(Cyanotis arachnoidea C.B.Clarke)对所含β-蜕皮激素释放的影响。[方法]采用GRACE ODS C18色谱柱(250 mm×4.6 mm,5μm),流动相甲醇∶水=40∶60(V/V,%)洗脱。柱温26℃,流速1.0 ml/min,检测波长248 nm。[结果]相同来源的露水草粉体(粒径10、30和50μm)β-蜕皮激素含量检测值和普通细粉(粒径180μm)相比,粉体中蜕皮激素激素的检测值平均高1倍多。[结论]露水草粉体中的蜕皮激素更容易溶于甲醇,其提取率明显高于普通细粉。表明露水草经过超微粉碎后,细胞破壁率到得明显提高,更有利于有效成分的溶出。