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Ethanol extract of Cyathulae Radix inhibits osteoclast differentiation and bone loss 被引量:1
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作者 SHI Liying REN Liuyi +7 位作者 LI Jinping LIU Xin LU Jingjing JIA Lujuan XIE Baoping TANG Siyuan LIU Wei ZHANG Jie 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2024年第3期212-223,共12页
Cyathulae Radix,a traditional Chinese medicine and a common vegetable,boasts a history spanning millennia.It enhances bone density,boosts metabolism,and effectively alleviates osteoporosis-induced pain.Despite its his... Cyathulae Radix,a traditional Chinese medicine and a common vegetable,boasts a history spanning millennia.It enhances bone density,boosts metabolism,and effectively alleviates osteoporosis-induced pain.Despite its historical use,the molecular mechanisms behind Cyathulae Radix’s impact on osteoporosis remain unexplored.In this study,we investigated the effects and mechanisms of Cyathulae Radix ethanol extract(CEE)in inhibiting osteoporosis and osteoclastogenesis.Eight-week-old female mice underwent ovariectomy and were treated with CEE for eight weeks.Micro-computed tomography(micro-CT)assessed histomorphometric parameters,bone tissue staining observed distal femur histomorphology,and three-point bending tests evaluated tibia mechanical properties.Enzyme-linked immunosorbent assay(ELISA)measured serum estradiol(E2),receptor activator for nuclear factor B ligand(RANKL),and osteoprotegerin(OPG)levels.Osteoclastogenesis-related markers were analyzed via Western blotting(WB)and quantitative real-time polymerase chain reaction(qRT-PCR).Additionally,CEE effects on RANKL-induced osteoclast formation and bone resorption were investigated in vitro using tartrate-resistant acid phosphatase(TRAP)staining,qRT-PCR,and WB assay.Compared with the ovariectomy(OVX)group,CEE treatment enhanced trabecular bone density,maximal load-bearing capacity,and various histomorphometric parameters.Serum E2 and OPG levels significantly increased,while Receptor activator of nuclear factor-κB(RANK)decreased in the CEE group.CEE downregulated matrix metallopeptidase 9(MMP-9),Cathepsin K(CTSK),and TRAP gene and protein expression.In bone marrow macrophages(BMMs),CEE reduced mature osteoclasts,bone resorption pit areas,and MMP-9,CTSK,and TRAP expression during osteoclast differentiation.Compared with DMSO treatment,CEE markedly inhibited RANK,TNF receptor associated factor 6(TRAF6),Proto-oncogene c-Fos(c-Fos),Nuclear factor of activated T-cells cytoplasmic 1(NFATc1)expressions,and Extracellular regulated protein kinases(ERK),c-Jun N-terminal kinase(JNK),NF-kappa B-p65(p65)phosphorylation in osteoclasts.In conclusion,CEE significantly inhibits OVX-induced osteoporosis and RANKL-induced osteoclastogenesis,potentially through modulating the Estrogen Receptor(ER)/RANK/NFATc1 signaling pathway. 展开更多
关键词 OSTEOPOROSIS OSTEOCLAST BMMs cyathulae radix RANKL ER/RANK/NFATc1 signaling pathway
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川牛膝片产地趁鲜加工及其可行性
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作者 郭晓亮 穆森 +2 位作者 段媛媛 胡昌强 黄浩 《湖北农业科学》 2024年第11期114-121,共8页
为了优化川牛膝(Cyathulae Radix)片产地趁鲜切制加工工艺并验证其可行性,采用正交试验设计,考察初干温度、初干程度、发汗时间及复干温度对川牛膝片质量与干燥时长的影响,并通过质量评价与代谢组分析验证工艺的可行性。结果表明,4个因... 为了优化川牛膝(Cyathulae Radix)片产地趁鲜切制加工工艺并验证其可行性,采用正交试验设计,考察初干温度、初干程度、发汗时间及复干温度对川牛膝片质量与干燥时长的影响,并通过质量评价与代谢组分析验证工艺的可行性。结果表明,4个因素均对干燥时长、浸出物含量存在显著或极显著影响;初干温度对杯苋甾酮含量、前杯苋甾酮含量存在显著影响;复干温度对前杯苋甾酮含量存在显著影响。川牛膝片产地趁鲜切制加工最佳工艺:川牛膝鲜品切为单支后,先于50℃干燥至含水量41.2%,再发汗15~30 h、切片,后于70℃干燥至含水量不高于12.0%。趁鲜川牛膝片质量优于市售饮片(SSP)与传统川牛膝片(CTP)。趁鲜川牛膝片(CXP)与传统川牛膝片的化学成分相似性很高;相对于CTP,筛选出的36种差异代谢物中,CXP中有7种黄酮类化合物、5种皂苷类化合物含量上调;有20种黄酮类化合物、2种皂苷类化合物和2种甾酮类化合物含量下调。因此,川牛膝片产地趁鲜加工取代传统方法在一定程度上是可行的。 展开更多
关键词 川牛膝(cyathulae radix)片 产地趁鲜加工 质量 代谢组 可行性
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