Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culici...Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.展开更多
Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could f...Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.展开更多
Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of ...Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.展开更多
In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of lea...In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.展开更多
In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. ...In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.展开更多
To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination...To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination of varieties of different colors were investigated. The main results indicate that the heredity of Cyclamen persicum color follows both quantitative and qualitative hereditary regularity. All genes can be classified as two groups of the throat-gene and coronal-gene occupying different spots on the chromosome. The colored gene is the dominant gene, while the white gene is the recessive one. The gene groups and their quantity determine the color of cyclamen persicum. And sometimes the throat- gene and the coronal-gene take a recombination, forming a new type of flower.展开更多
在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其...在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其中1.0 m g/L TDZ对叶片再生芽的诱导效果最好,而子叶以0.5 m g/LTDZ、子叶节和叶柄以0.2 m g/L TDZ诱导再生芽的效果较好。展开更多
基金the Scientific Projects Administration Unit of Akdeniz University.Turkey for providing financial support
文摘Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.
文摘Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.
文摘Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.
基金Supported by"Introduction of Germplasm Resources and the Distant Hybridization Techniques of Cyclamen Hederifolium"of"948"Introduced Project of State Forestry Bureau(2013-4-42)
文摘In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.
文摘In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.
基金The project was funded by Hebei Forestry Bureau (0008261)
文摘To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination of varieties of different colors were investigated. The main results indicate that the heredity of Cyclamen persicum color follows both quantitative and qualitative hereditary regularity. All genes can be classified as two groups of the throat-gene and coronal-gene occupying different spots on the chromosome. The colored gene is the dominant gene, while the white gene is the recessive one. The gene groups and their quantity determine the color of cyclamen persicum. And sometimes the throat- gene and the coronal-gene take a recombination, forming a new type of flower.
文摘在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其中1.0 m g/L TDZ对叶片再生芽的诱导效果最好,而子叶以0.5 m g/LTDZ、子叶节和叶柄以0.2 m g/L TDZ诱导再生芽的效果较好。
