Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could f...Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.展开更多
Objective:To evaluate the effect of Heracleum persicum L.against gentamicin-induced nephrotoxicity in rats.Methods:Thirty-six Wistar rats were divided into 6 groups including control(normal saline),gentamicin(80 mg/kg...Objective:To evaluate the effect of Heracleum persicum L.against gentamicin-induced nephrotoxicity in rats.Methods:Thirty-six Wistar rats were divided into 6 groups including control(normal saline),gentamicin(80 mg/kg/d for 10 d),Heracleum persicum(750 mg/kg/d),and gentamicin(10 d)+Heracleum persicum extract at three different doses(250,500,and 750 mg/kg/d for 40 d).Urine creatinine,urea,protein,and albumin levels were determined.In addition,serum urea,creatinine,sodium,potassium,cytokines(TNF-α,IL-1β,IL-6,and IL-10),glutathione peroxidase activity,total antioxidant capacity,kidney malondialdehyde,stereological parameters,and expressions of apoptosis-related genes(p53,Bax,Bcl-2,and caspase-3)were measured.The LD50 of Heracleum persicum extract was determined based on Lorke’s method.Histopathological evaluation was also performed.Results:In addition to decreased urine protein and albumin,and increased creatinine and urea,co-treatment with gentamicin and Heracleum persicum significantly reduced levels of creatinine and urea,and increased sodium and potassium in serum.Heracleum persicum treatment also improved stereological parameters and serum inflammatory cytokines.There was a significant increase in serum glutathione peroxidase activity and total antioxidant capacity as well as a reduction in malondialdehyde level.Furthermore,treatment with Heracleum persicum extracts downregulated p53,caspase-3,and Bax and upregulated Bcl-2 expressions.In histopathological evaluation,Heracleum persicum extracts showed protection against gentamicin-induced renal damages.Conclusions:Heracleum persicum exhibits protective effects against gentamicin-induced structural and functional renal impairments.展开更多
Competition,spatial pattern,and regeneration are important factors affecting community composition,structure,and dynamics.In this study,we surveyed 300 quadrats from three dunes(i.e.,fixed dunes,semifixed dunes,and mo...Competition,spatial pattern,and regeneration are important factors affecting community composition,structure,and dynamics.In this study,we surveyed 300 quadrats from three dunes(i.e.,fixed dunes,semifixed dunes,and mobile dunes)in the Gurbantunggut Desert,Northwest China,from late May to early June in 2021.The intraspecific and interspecific competition,spatial pattern,and regeneration of Haloxylon ammodendron and Haloxylon persicum were studied using the Hegyi competition index and point pattern analysis methods.The results showed that the optimal competition distance of the objective tree in the H.ammodendron and H.persicum communities was 6 m.The intraspecific and interspecific competition of H.ammodendron was the greatest in fixed dunes,while the competition intensity of H.persicum in semifixed dunes and mobile dunes was greater than that in fixed dunes.The order of competition intensity of the two populations was seedlings>saplings>adults,and the competition intensity gradually decreased with the increase in plant diameter.The spatial distribution pattern of the three life stages of H.ammodendron and H.persicum was random,and there were no correlations between seedlings and saplings,adults and saplings,and seedlings and adults.The density of regenerated seedlings and saplings of H.ammodendron in the three dunes followed the order of fixed dunes>semifixed dunes>mobile dunes,and that of H.persicum in the three dunes followed the order of mobile dunes>semifixed dunes>fixed dunes.Therefore,when artificially planting H.ammodendron and H.persicum for sand control,the planting interval should be 6 m,and seedlings should be planted next to adults to minimize the competition between plants,which can promote the renewal of H.ammodendron and H.persicum and the stabilization of the ecosystem.展开更多
Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culici...Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.展开更多
Objective:To develop a protocol lor breaking of seed dormancy and increasing the seed germination rate of Bunium persicum.Methods:The seeds were treated with 3.1.6.3.12.5.25.50 and 100 μmol/L of benzyl aminopurine.gi...Objective:To develop a protocol lor breaking of seed dormancy and increasing the seed germination rate of Bunium persicum.Methods:The seeds were treated with 3.1.6.3.12.5.25.50 and 100 μmol/L of benzyl aminopurine.gibberellic acid(GA,),thidiazuron(TDZ) and forchlorlenuron.Then,seeds were transferred to two different temperature conditions including room temperature(25 ℃) and chilling temperature(2-5℃).Results:The treatment of moist seeds with chilling temperature(2—5℃) broke seed dormancy and showed maximum germination,which was 54.7%after 60 d treatment.Also,the treatment of dn seeds with chilling temperature broke seed dormancy with 9.3%germination rate after 120 d.Treatment of seeds with different level of plant growth regulators showed that under moistroom condition,there was evidence ol higher and lower seed germination rate:GA,(100 μmol/L)with 46.7%and TDZ(50 μmol/L) with 6.67%respectively.In addition,the results showed that under moist-chilling condition.TDZ(6.3 μmol/L) with 53.3%seed germination rate had higher influence on breaking seed dormancy.Treatment of seeds with combination of TDZ and GA_3 under moistchilling condition revealed higher rale of breaking of seed dormancy when 6.3 μmol/L TDZ was combined with 100 μmol/L GA,.showing 93.7%genninatiou rate.Conclusions:The effect of plant growth regulators coupled with chilling temperature on breaking of seed dormancy could provide a large number of seedlings while the long juvenile time which is the next restricting factor of plantation still remained.Thus,the subsequent growth of seedlings to provide a large number of corms is necessary for successful plantation.展开更多
Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of ...Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.展开更多
In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of lea...In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.展开更多
In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. ...In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.展开更多
文摘Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.
基金financially supported by Kermanshah University of Medical Science(grant number 981004)
文摘Objective:To evaluate the effect of Heracleum persicum L.against gentamicin-induced nephrotoxicity in rats.Methods:Thirty-six Wistar rats were divided into 6 groups including control(normal saline),gentamicin(80 mg/kg/d for 10 d),Heracleum persicum(750 mg/kg/d),and gentamicin(10 d)+Heracleum persicum extract at three different doses(250,500,and 750 mg/kg/d for 40 d).Urine creatinine,urea,protein,and albumin levels were determined.In addition,serum urea,creatinine,sodium,potassium,cytokines(TNF-α,IL-1β,IL-6,and IL-10),glutathione peroxidase activity,total antioxidant capacity,kidney malondialdehyde,stereological parameters,and expressions of apoptosis-related genes(p53,Bax,Bcl-2,and caspase-3)were measured.The LD50 of Heracleum persicum extract was determined based on Lorke’s method.Histopathological evaluation was also performed.Results:In addition to decreased urine protein and albumin,and increased creatinine and urea,co-treatment with gentamicin and Heracleum persicum significantly reduced levels of creatinine and urea,and increased sodium and potassium in serum.Heracleum persicum treatment also improved stereological parameters and serum inflammatory cytokines.There was a significant increase in serum glutathione peroxidase activity and total antioxidant capacity as well as a reduction in malondialdehyde level.Furthermore,treatment with Heracleum persicum extracts downregulated p53,caspase-3,and Bax and upregulated Bcl-2 expressions.In histopathological evaluation,Heracleum persicum extracts showed protection against gentamicin-induced renal damages.Conclusions:Heracleum persicum exhibits protective effects against gentamicin-induced structural and functional renal impairments.
基金the Open Project of Xinjiang Laboratory of Lake Environment and Resources in Arid Zone(XJDX0909-2022-4)the PhD Early Development Program of Xinjiang Normal University(XJNUBS2113).
文摘Competition,spatial pattern,and regeneration are important factors affecting community composition,structure,and dynamics.In this study,we surveyed 300 quadrats from three dunes(i.e.,fixed dunes,semifixed dunes,and mobile dunes)in the Gurbantunggut Desert,Northwest China,from late May to early June in 2021.The intraspecific and interspecific competition,spatial pattern,and regeneration of Haloxylon ammodendron and Haloxylon persicum were studied using the Hegyi competition index and point pattern analysis methods.The results showed that the optimal competition distance of the objective tree in the H.ammodendron and H.persicum communities was 6 m.The intraspecific and interspecific competition of H.ammodendron was the greatest in fixed dunes,while the competition intensity of H.persicum in semifixed dunes and mobile dunes was greater than that in fixed dunes.The order of competition intensity of the two populations was seedlings>saplings>adults,and the competition intensity gradually decreased with the increase in plant diameter.The spatial distribution pattern of the three life stages of H.ammodendron and H.persicum was random,and there were no correlations between seedlings and saplings,adults and saplings,and seedlings and adults.The density of regenerated seedlings and saplings of H.ammodendron in the three dunes followed the order of fixed dunes>semifixed dunes>mobile dunes,and that of H.persicum in the three dunes followed the order of mobile dunes>semifixed dunes>fixed dunes.Therefore,when artificially planting H.ammodendron and H.persicum for sand control,the planting interval should be 6 m,and seedlings should be planted next to adults to minimize the competition between plants,which can promote the renewal of H.ammodendron and H.persicum and the stabilization of the ecosystem.
基金the Scientific Projects Administration Unit of Akdeniz University.Turkey for providing financial support
文摘Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.
基金Supported by Universiti Putra Malaysia.the project via Research University Grant Scheme(RUGS)(Vote No.9322400)
文摘Objective:To develop a protocol lor breaking of seed dormancy and increasing the seed germination rate of Bunium persicum.Methods:The seeds were treated with 3.1.6.3.12.5.25.50 and 100 μmol/L of benzyl aminopurine.gibberellic acid(GA,),thidiazuron(TDZ) and forchlorlenuron.Then,seeds were transferred to two different temperature conditions including room temperature(25 ℃) and chilling temperature(2-5℃).Results:The treatment of moist seeds with chilling temperature(2—5℃) broke seed dormancy and showed maximum germination,which was 54.7%after 60 d treatment.Also,the treatment of dn seeds with chilling temperature broke seed dormancy with 9.3%germination rate after 120 d.Treatment of seeds with different level of plant growth regulators showed that under moistroom condition,there was evidence ol higher and lower seed germination rate:GA,(100 μmol/L)with 46.7%and TDZ(50 μmol/L) with 6.67%respectively.In addition,the results showed that under moist-chilling condition.TDZ(6.3 μmol/L) with 53.3%seed germination rate had higher influence on breaking seed dormancy.Treatment of seeds with combination of TDZ and GA_3 under moistchilling condition revealed higher rale of breaking of seed dormancy when 6.3 μmol/L TDZ was combined with 100 μmol/L GA,.showing 93.7%genninatiou rate.Conclusions:The effect of plant growth regulators coupled with chilling temperature on breaking of seed dormancy could provide a large number of seedlings while the long juvenile time which is the next restricting factor of plantation still remained.Thus,the subsequent growth of seedlings to provide a large number of corms is necessary for successful plantation.
文摘Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.
基金Supported by"Introduction of Germplasm Resources and the Distant Hybridization Techniques of Cyclamen Hederifolium"of"948"Introduced Project of State Forestry Bureau(2013-4-42)
文摘In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.
文摘In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.
