The Impact Heme Oxygenase -1 on the Regulating Factors of Hepatoma Cells＇ Cell Cycle

It aims to analyze the impact heme oxygenase -1 （heme oxygenase 1, HO-1） on regulating factors of human hepatoma cell HepG2＇s cell cycle, through constructing recombinant vector of pcDNA3.1 containing wild-type and mutant HO-1 gene （＋）-wtHO-1 and pcDNA3.1 （＋）-mHO-1G143H. By using the method of liposome-mediated, the recombinant vector was transfected hepatoma cell line HepG2. And the transfected one with empty vector was treated as a control group. By the selection of G418, stable expression of wild-type and mutant HO-1 in HepG2 liver cancer cell lines were established. Use the blot of semi-quantitative RT-PCR and Western to test transfected cell lines expressing levels of riO-1 mRNA and protein. As HO-1 expression in stably transfected cell lines altered, we use Western blot to test transfected cell lines P21, P27 protein expression levels. As result shows, we got 1 HO-over-expression of wild-type and mutant in HepG2 cells; wild- type and mutant＇s over expression of HO-1 can induce the expression of tumor suppressor genes p21 and p27.we got the conclusion that HO-l＇s over-expression of tumor suppressor genes p21 and p27 is unrelated to the expression of heme decomposition products. HO-1 may regulate the expression of p21 and p27 through other mechanisms.

<i>Supereulerian Digraph</i>Strong Products

A vertex cycle cover of a digraph H is a collection C = {C1, C2, …, Ck} of directed cycles in H such that these directed cycles together cover all vertices in H and such that the arc sets of these directed cycles induce a connected subdigraph of H. A subdigraph F of a digraph D is a circulation if for every vertex in F, the indegree of v equals its out degree, and a spanning circulation if F is a cycle factor. Define f (D) to be the smallest cardinality of a vertex cycle cover of the digraph obtained from D by contracting all arcs in F, among all circulations F of D. Adigraph D is supereulerian if D has a spanning connected circulation. In [International Journal of Engineering Science Invention, 8 (2019) 12-19], it is proved that if D1 and D2 are nontrivial strong digraphs such that D1 is supereulerian and D2 has a cycle vertex cover C’ with |C’| ≤ |V (D1)|, then the Cartesian product D1 and D2 is also supereulerian. In this paper, we prove that for strong digraphs D1 and D2, if for some cycle factor F1 of D1, the digraph formed from D1 by contracting arcs in F1 is hamiltonian with f (D2) not bigger than |V (D1)|, then the strong product D1 and D2 is supereulerian.

COMBINED DETECTION OF CYCLIN D1,P27 AND DNA CONTENT IN ESOPHAGEAL CANCER

Objective： To investigate the expressions of cyclin D1 and p27 and DNA content in esophageal cancer and adjacent normal tissues, and to discuss the relationship between them. Methods： The cyclinD1 and p27 were detected by immunohistochernical staining; DNA content was measured by flow cytometry. Results： The positive expression rates of cyclinD1 and p27 in cancer were 45.8% and 33.3% respectively, the DNA content in the positive group of cyclinD1 was higher than that in the negative group of cyclinDl（1.54±0.21 versus 1.08i-0.43, P〈0.05）, while the DNA content and SPF （S-phase fraction） in the positive group of p27 were lower than those in the negative group （1.10±0.19 and 5.56%±5.18% versus 1.66±0.28 and 19.78%±6.12%, P〈0.05）. Conclusion： The data show that the expression of cyclinD1 and p27 are related to the ontogenesis and progression of esophageal cancer. The combined detection of cyclinD1, p27 and DNA content may be indicators of diagnosis and assessment of esophageal cancer.

An Experimental Study on Constant Current Load Test of Reinforced Concrete based on 3D Paraffin Isolation

Aiming at the complex corrosion degradation factors of reinforced concrete and clearing the deterioration mechanism in the constant stress state,a new type of constant current accelerated corrosion method in the saline soil environment was developed.The three-dimensional paraffin isolation specimens and the three-dimensional penetration specimens were taken as the research objects,and the Cl−content and AC impedance Bode diagram were measured.The macro morphology and micro analysis were also used to evaluate the corrosion degradation laws of the two groups of specimens.A constant current three-factor system accelerated model was established for the current acceleration factor,chloride ion,and sulfate ion acceleration factor.The experimental results show that,in the constant stress test of the saline soil environmental conditions,the paraffin isolation layer can effectively isolate corrosive chloride ions,which is a brand-new research method of single factor variable control in the constant stress test.According to the basic corrosion data,the law of constant current acceleration test is summarized and divided into five corrosion degradation stages,and each stage has significant changes in the accelerated corrosion efficiency.The corrosion degradation of a constant stress test is the combined effect of constant current,positive and negative penetration of chloride ions and sulfate ions.

Effects of insulin-like growth factor binding protein 7 on cell cycle and proliferation of gastric cancer in vitro and in vivo

Objective To investigate the effects of insulin-like growth factor binding protein 7(IGFBP7)on the proliferation,cell cycle of gastric cancer cell and the expression of cynlin D1,cyclin-dependent kinase(CDK)4,and to observe the effects of IGFBP7 on the growth of gastric tumor xenografts in nude mice.Methods The MKN-28cell line was interfered by small interfere ribonucleic acid(siRNA)(interfered group),and blank control group,