The development of sustainable techniques to produce high-performance zeolite is essential to achieve green production in industry.Herein,we report an eco-friendly route to synthesizing hierarchical Beta zeolite from ...The development of sustainable techniques to produce high-performance zeolite is essential to achieve green production in industry.Herein,we report an eco-friendly route to synthesizing hierarchical Beta zeolite from kaolinite and recycled mother liquor.The results reveal that the unutilized species(such as silicon species and Na+)in mother liquor stayed in a stable concentration during eleven recycled experiments.Moreover,the synthesized Beta zeolites still have comparable physicochemical properties and catalytic performance in the esterification of levulinic acid with ethanol over the initial zeolite although eleven recycled experiments.Life cycle assessment exhibits that the synthesis of Beta zeolite with recycled mother liquor can reduce global warming potential by 23%and resource depletion-water use by 36%compared to that without recycled mother liquor.This quantitatively demonstrates that the approach proposed in this work is really a sustainable one,extremely increasing the utilization efficiency of raw materials and decreasing the environmental burden.展开更多
The reuse of waste recycled concrete from harsh environments has become a research hotspot in the field of construction.This study investigated the repair effect of carbonation treatment on second-generation recycled ...The reuse of waste recycled concrete from harsh environments has become a research hotspot in the field of construction.This study investigated the repair effect of carbonation treatment on second-generation recycled fine aggregate(SRFA)obtained from recycled fine aggregate concrete(RFAC)subjected to freeze-thaw(FT)cycles.Before and after carbonation,the properties of SRFA were evaluated.Carbonated second-generation recycled fine aggregate(CSRFA)at five substitution rates(0%,25%,50%,75%,100%)to replace SRFA was used to prepare carbonated second-generation recycled fine aggregate concrete(CSRFAC).The water absorption,porosity and mechanical properties of CSRFAC were tested,and its frost-resisting durability was evaluated.The results showed after carbonation treatment,the physical properties of SRFA was improved and met the requirements of II aggregate.The micro-hardness of the interfacial transition zone and attached mortar in CSRFA was 50.5%and 31.2%higher than that in SRFA,respectively.With the increase of CSRFA replacement rate,the water absorption and porosity of CSRFAC gradually decreased,and the mechanical properties and frost resistance of CSRFAC were gradually improved.Carbonation treatment effectively repairs the damage of SRFA caused by FT cycles and improves its application potential.展开更多
With the emphasis on environmental issues,the recycling of waste concrete,even recycled concrete,has become a hot spot in the field of architecture.But the repeated recycling of waste concrete used in harsh environmen...With the emphasis on environmental issues,the recycling of waste concrete,even recycled concrete,has become a hot spot in the field of architecture.But the repeated recycling of waste concrete used in harsh environments is still a complex problem.This paper discusses the durability and recyclability of recycled aggregate concrete(RAC)as a prefabricated material in the harsh environment,the effect of high-temperature curing(60℃,80℃,and 100℃)on the frost resistance of RAC and physical properties of the second generation recycled coarse aggregate(RCA_(2))of RAC after 300 freeze-thaw cycles were studied.The frost resistance of RAC was characterized by compressive strength,relative dynamic elastic modulus,and mass loss.As the physical properties of RCA_(2),the apparent density,water absorption,and crushing value were measured.And the SEM images of RAC after 300 freeze-thaw cycles were shown.The results indicated that the frost resistance of RAC cured at 80℃ for 7 days was comparable to that cured in the standard condition(cured for 28 days at 20℃±2℃ and 95%humidity),and the RAC cured at 100℃ was slightly worse.However,the frost resistance of RAC cured at 60℃ deteriorated seriously.The RAC cured at 80℃ for 7 days is the best.Whether after the freeze-thaw cycle or not,the RCA that curd at 60℃,80℃,and 100℃ for 7 days can also meet the requirements of Grade III RCA and be used as the aggregate of non-bearing part of prefabricated concrete components.RCA_(2) which is cured at 80℃ for 7 days had the best physical properties.展开更多
1 Introduction Low-dimensional nanostructures, such as nanorods, nanowires, and nanotubes, have received much attention for their superior optical, electrical, catalytic and magnetic properties. Owing to their low di...1 Introduction Low-dimensional nanostructures, such as nanorods, nanowires, and nanotubes, have received much attention for their superior optical, electrical, catalytic and magnetic properties. Owing to their low dimensionality and quantum continement effect, low-dimensional nanoscale materials can be exploited as fundamental building blocks for nanoscience and nanodevices^[1-3]. In recent years, efforts have been devoted to develop new approaches to synthesize one-dimensional(lD) nanostructrued vanadium oxides or vanadates materials, such as V205, NaV2Os, and CuV206, which have been widely investigated in catalytic or electrochemical fields due to their outstanding structural flexibility^[4-6].展开更多
The degradation progress of recycled aggregate concrete(RAC) subjected to sulfate attack under wetting-drying cycles and flexural loading is studied. Three different stress ratios(0, 0.3 and 0.5) were applied in this ...The degradation progress of recycled aggregate concrete(RAC) subjected to sulfate attack under wetting-drying cycles and flexural loading is studied. Three different stress ratios(0, 0.3 and 0.5) were applied in this test. The variations of relative dynamic elastic modulus Erd and water-soluble SO2-4 contents in RAC were used to evaluate degradation progress. The changes in mineral products and microstructures of interior concrete were investigated by means of X-ray diffraction(XRD), the environmental scanning electron microscope(ESEM) and X-ray computed tomography(X-CT). The results indicate that flexural loading accelerates the degradation of RAC under sulfate attack and wetting-drying cycles by expediting the transmission of SO2-4 into interior concrete. Furthermore, the accelerated effect of flexural loading is more obvious with the increase of stress ratio, that is because higher stress ratios can accelerate the extension of microcracks and generate more channels for the transmission of SO2-4. Also, more expansive products(gypsum and ettringite) are generated by the reaction of Ca(OH)2 with SO2-4, which can further accelerate the degradation of RAC.展开更多
This work examines the environmental and geochemical impact of recycled aggregate concrete production with properties representative for structural applications.The environmental influence of cement content,aggregate ...This work examines the environmental and geochemical impact of recycled aggregate concrete production with properties representative for structural applications.The environmental influence of cement content,aggregate production,transportation,and waste landfilling is analysed by undertaking a life cycle assessment and considering a life cycle inventory largely specific for the region.To obtain a detailed insight into the optimum life cycle parameters,a sensitivity study is carried out in which supplementary cementitious materials,different values of natural-to-recycled aggregate content ratio and case-specific transportation distances were considered.The results show that carbon emissions were between 323 and 332 kgCO_(2)e per cubic metre of cement only natural aggregate concrete.These values can be reduced by up to 17%by replacing 25%of the cement with fly ash.By contrast,carbon emissions can increase when natural coarse aggregates are replaced by recycled aggregates in proportions of 50%and 100%,and transportation is not included in analysis.However,the concrete with 50%recycled aggregate presented lower increase,only 0.3%and 3.4%for normal and high strength concrete,respectively.In some cases,the relative contribution of transportation to the total carbon emissions increased when cement was replaced by fly ash in proportions of 25%,and case-specific transportation distances were considered.In absolute values,the concrete mixes with 100%recycled aggregates and 25%fly ash had lower carbon emissions than concrete with cement and natural aggregates only.Higher environmental benefits can be obtained when the transportation distances of fly ash are relatively short(15–25 km)and the cement replacement by fly ash is equal or higher than 25%,considering that the mechanical properties are adequate for practical application.The observations from this paper show that recycled aggregate concrete with strength characteristics representative for structural members can have lower carbon emissions than conventional concrete,recommending them as an alternative to achieving global sustainability standards in construction.展开更多
The objective of this project was to characterize the freeze-thaw properties of recycled concrete(RCA)and asphalt(RAP)as unbound base and to assess how they behaved in the field for nearly 8 years.This paper includes ...The objective of this project was to characterize the freeze-thaw properties of recycled concrete(RCA)and asphalt(RAP)as unbound base and to assess how they behaved in the field for nearly 8 years.This paper includes an examination of existing information,laboratory studies of freeze-thaw behavior,and evaluation of data from Mn ROAD field-test sections in a seasonally cold region,i.e.,in Minnesota,USA.Test sections were constructed using recycled materials in the granular base layers at the Mn ROAD test facility.One test section included 100%RAP,another 100%RCA,a third one a 50/50blend of RCA/natural aggregate,and a fourth one only natural aggregate(Class 5)as a control.The stiffness(i.e.,elastic modulus)was monitored during construction and throughout the pavement life by the Minnesota Department of Transportation,along with the variation of temperatures and moisture regimes in the pavement to determine their effects on pavement performance.The resilient modulus of each material was determined by bench-scale testing in accordance with NCHRP 1-28a,as well as by field-scale tests incorporating a falling-weight deflectometer.Specimens were subjected to as many as 20 cycles of freeze-thaw in the laboratory,and the change in their resilient modulus was measured.In the field-test sections constructed with the same materials as the base course,temperature,moisture,and field modulus(from fallingweight deflectometer tests)were monitored seasonally for nearly 8 years.From the temperatures in the base course layer,the number of freeze-thaw cycles experienced in the field was determined for each test section.Inferences were made relative to modulus change versus freeze-thaw cycles.Conclusions were drawn for long-term field performances of the recycled base(RAB)in comparison to natural aggregate.展开更多
The paper studied the relationship between microstructure and shape recovery characteristics by using colored microstructure analysis under polarized light on the thermomechanical cycled CuAlNi single crystals. The tw...The paper studied the relationship between microstructure and shape recovery characteristics by using colored microstructure analysis under polarized light on the thermomechanical cycled CuAlNi single crystals. The two-way shape memory effect in quenched thin bar resulted from the preferential formation/extinction of martensite variant due to the internal quench stress, and the variant was formed at an angle of about 45 deg. with the tension direction ([001] of the βphase). Initial thermomechanical cycling under relatively low stress single variant stress-induced martensite was formed at an angle of 45 deg. with the tension and its morphology was a lath of parallel twins. More than one group of variants were formed after several training cycles and such variants also caused tilting of some thermally formed accommodated martensite. By overheating the trained sample containing stabilized multi-variants of stress-induced martensite, very coarse martensite structure with a strong asymmetry was produced, which caused the reverse two-way shape memory effect.展开更多
A new LiCoO2 recovery technology for Li-ion batteries was studied in this paper. LiCoO2 was peeled from the Al foil with dimethyl acetamide (DMAC), and then polyvinylidene fluoride (PVDF) and carbon powders in the...A new LiCoO2 recovery technology for Li-ion batteries was studied in this paper. LiCoO2 was peeled from the Al foil with dimethyl acetamide (DMAC), and then polyvinylidene fluoride (PVDF) and carbon powders in the active material were eliminated by high temperature calcining. Subsequently, Li2CO3, LiOH-H20 and LiAc-2H2O were added into the recycled powders to adjust the Li/Co molar ratio to 1.00. The new LiCoO2 was obtained by calcining the mixture at 850℃ for 12 h in air. The structure and morphology of the recycled powders and resulting samples were studied by XRD and SEM techniques, respectively. The layered structure of LiCoO2 synthesized by adding Li2CO3 is the best, and it is found to have the best characteristics as a cathode material in terms of charge-discharge capacity and cycling performance. The first discharge capacity is 160 mAh·g^-1 between 3.0-4.3 V. The discharge capacity after cycling for 50 times is still 145.2 mAh·g^-1.展开更多
Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular compone...Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular components in the circulating water. The objective of the simulation is to determine the maximum allowable concentrations of ions and molecules as well as the choice of conditions for deposition or adsorption.First of all, our examinations decrease the concentration of copper ions and fatty acids in the circulating water. By pre-mixing water with the highest concentration of these ions, a reduction of copper ion and fatty acid concentrations in the recycled water occurs. The results do not only ensure the achievement of the maximum permitted concentration(MPC) of copper and iron, significantly reducing the amount of oxidized copper, they also make it possible to use the united sewage as current water for the flotation process. Mixing and adding filtrate of tailings, discharges of urban wastewater treatment and effluent of ash pit of thermal power stations(TPS) to recycled water causes an increase in the capacity of the enrichment plant by 15–17%.展开更多
The mechanical properties of polyvinyl alcohol (PVA) films prepared by evaporating water from freeze/thaw cycled gel were investigated as a function of the number of freeze/thaw cycles. The maximum stress of the PVA f...The mechanical properties of polyvinyl alcohol (PVA) films prepared by evaporating water from freeze/thaw cycled gel were investigated as a function of the number of freeze/thaw cycles. The maximum stress of the PVA film prepared by freeze/thaw cycling was larger than that prepared without the freeze/thaw cycle process. The largest maximum stress was 46.2 MPa for a film prepared with 10 freeze/thaw cycles, which was twice as large as that for a cast PVA film without freeze/thaw cycling (22.3 MPa). This is due to the formation of small crystallites during the freeze/thaw cycle process. Furthermore, when the film was annealed at 130°C, the maximum stress was as high as 181 MPa which was comparable to that for PVA films prepared using additives. The crystallinity is not the main factor that determines the maximum stress for either the non-annealed or annealed freeze/thaw cycled films, but the glass transition temperature is well correlated with the maximum stress, irrespective of the annealing process. This is due to the different molecular morphology;the non-annealed freeze/thaw cycled film consists of many small crystallites, but the annealed film consists of larger crystallites formed during the annealing process.展开更多
Wake-up radio (WuR) system is often presented as the best candidate for replacing traditional duty cycled Medium Access Control (MAC) protocols in Wireless Sensor Networks (WSNs). The Double Radio (DoRa) protocol is a...Wake-up radio (WuR) system is often presented as the best candidate for replacing traditional duty cycled Medium Access Control (MAC) protocols in Wireless Sensor Networks (WSNs). The Double Radio (DoRa) protocol is a new MAC protocol for in-band WuR system with addressing capabilities. While the DoRa protocol improves the WSNs energy efficiency, it still suffers from an overhearing problem when the WuR system is very often requested. The WuR wastes a noticeable amount of energy when overhearing to wake-up demand intended to other nodes, but it is neither measured nor solved in other works. In this paper, an adaptive duty-cycled DoRa (DC-DoRa) is then proposed to solve the overhearing problem. The primary concept of the work is to enable the WuR functionality before the node is addressed and to disable the WuR after the node sent data. Extensive simulations under OMNeT++ using real input parameters are then performed to show the significant energy-savings through the two protocols and the nearly suppression of overhearing with DC-DoRa. In fact, the mean power consumption is three-order below using the DoRa protocol compared to traditional MAC protocols. While overhearing can represent up to 93% of the WuR energy consumption with the DoRa protocol, it is reduced to only 1% with the DC-DoRa protocol.展开更多
Optimized macroscopic tribological behavior can be anticipated in metallic glasses(MGs)by cryogenic cycling treatment(CCT),which is attributed to enhanced plasticity.However,the intrinsic friction mechanisms of MGs in...Optimized macroscopic tribological behavior can be anticipated in metallic glasses(MGs)by cryogenic cycling treatment(CCT),which is attributed to enhanced plasticity.However,the intrinsic friction mechanisms of MGs induced by cryogenic rejuvenation are still poorly understood.In the present study,nanoscopic wear tests were conducted on the Zr-based MGs surface with different CCT cycles using atomic force microscopy(AFM).After CCT treatment with 100 cycles,the MG displays the highest adhesion and ploughing frictions,but significantly improved anti-wear properties.Adhesion tests and molecular dynamics simulations disclose that the increased adhesion is attributed to the dominance of liquid-like regions in the CCT-treated MGs,and the impact of reduced hardness and weak elastic recovery results in the deteriorated ploughing friction.The enhanced plasticity effectively dissipates the strain from the AFM tip through multiple shear bands and weakens the adhesion during deformation,giving rise to excellent wear resistance.This study elucidates the promoting effect of CCT on the outstanding antiwear performance of MGs,and is helpful for the development of novel alloys.展开更多
The indiscriminate utilization of nondegradable polyethylene terephthalate(PET)-based products has triggered serious environmental pollution that has to be resolved vigorously.A simple synthesis of N-doped carbon nano...The indiscriminate utilization of nondegradable polyethylene terephthalate(PET)-based products has triggered serious environmental pollution that has to be resolved vigorously.A simple synthesis of N-doped carbon nanotubes from recycled PET(NCNTs_(r-PET))was developed by a nitric acid-assisted hydrothermal method.Experimental results and theoretical calculations show that the intrinsic defects in CNTs_(r-PET)would induce N-doping by NH_(3)generated from nitric acid during the hydrothermal process,thus producing the NCNTs_(r-PET).The life cycle assessment proves that the developed method for N-doped CNTs using r-PET as the carbon source is more environmentally friendly than the conventional chemical vapor deposition using acetylene as the carbon source.As a typical application,the NCNTs_(r-PET)delivered an impressive sodium storage capacity with an ultralong lifespan.This work not only provides a new route to upcycling waste plastics into valuable carbonaceous materials in an ecofriendly manner,but also reveals a basic understanding of the N-doping mechanism in carbonaceous materials.展开更多
Enzyme-induced carbonate precipitation(EICP)is an emanating,eco-friendly and potentially sound technique that has presented promise in various geotechnical applications.However,the durability and microscopic character...Enzyme-induced carbonate precipitation(EICP)is an emanating,eco-friendly and potentially sound technique that has presented promise in various geotechnical applications.However,the durability and microscopic characteristics of EICP-treated specimens against the impact of drying-wetting(D-W)cycles is under-explored yet.This study investigates the evolution of mechanical behavior and pore charac-teristics of EICP-treated sea sand subjected to D-W cycles.The uniaxial compressive strength(UCS)tests,synchrotron radiation micro-computed tomography(micro-CT),and three-dimensional(3D)recon-struction of CT images were performed to study the multiscale evolution characteristics of EICP-reinforced sea sand under the effect of D-W cycles.The potential correlations between microstructure characteristics and macro-mechanical property deterioration were investigated using gray relational analysis(GRA).Results showed that the UCS of EICP-treated specimens decreases by 63.7% after 15 D-W cycles.The proportion of mesopores gradually decreases whereas the proportion of macropores in-creases due to the exfoliated calcium carbonate with increasing number of D-W cycles.The micro-structure in EICP-reinforced sea sand was gradually disintegrated,resulting in increasing pore size and development of pore shape from ellipsoidal to columnar and branched.The gray relational degree suggested that the weight loss rate and UCS deterioration were attributed to the development of branched pores with a size of 100-1000 m m under the action of D-W cycles.Overall,the results in this study provide a useful guidancee for the long-term stability and evolution characteristics of EICP-reinforced sea sand under D-W weathering conditions.展开更多
A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the mol...A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the molten salt reactor and power system.This model considers the impact of thermal properties of fluid variation on accuracy and has been validated with Simulink.This study reveals the capability of the control system to compensate for anomalous situations and maintain shaft stability in the event of perturbations occurring in high-temperature molten salt tank outlet parameters.Meanwhile,the control system’s impact on the system’s dynamic characteristics under molten salt disturbance is also analyzed.The results reveal that after the disturbance occurs,the controlled system benefits from the action of the control,and the overshoot and disturbance amplitude are positively correlated,while the system power and frequency eventually return to the initial values.This simulation model provides a basis for utilizing molten salt reactors for power generation and maintaining grid stability.展开更多
To meet the goal of worldwide decarbonization,the transformation process toward clean and green energy structures has accelerated.In this context,coal-fired power plant(CFPP)and large-scale energy storage represented ...To meet the goal of worldwide decarbonization,the transformation process toward clean and green energy structures has accelerated.In this context,coal-fired power plant(CFPP)and large-scale energy storage represented by compressed air energy storage(CAES)technology,are tasked with increasing renewable resource accommodation and maintaining the power system security.To achieve this,this paper proposes the concept of a CFPP-CAES combined cycle and a trigenerative system based on that.Considering the working conditions of the CFPP,thermal characteristics of three typical operation modes were studied and some general regularities were identified.The results of various potential integration schemes discussion indicated that extracting water from low-temperature points in the feedwater system to cool pressurized air and simultaneously increase the backwater temperature is beneficial for improving performance.In addition,preheating the pressurized air before the air expanders via lowgrade water in the feedwater system as much as possible and reducing extracted steam contribute to increasing the efficiency.With the optimal integration scheme,2.85 tonnes of coal can be saved per cycle and the round-trip efficiency can be increased by 2.24%.Through the cogeneration of heat and power,the system efficiency can reach 77.5%.In addition,the contribution degree of the three compression heat utilization methods to the performance improvement ranked from high to low,is preheating the feedwater before the boiler,supplying heat,and flowing into the CFPP feedwater system.In the cooling energy generation mode,the system efficiency can be increased to over 69%.Regardless of the operation mode,the benefit produced by integration is further enhanced when the CFPP operates at higher operating conditions because the coupling points parameters are changed.In addition,the dynamic payback period can be shortened by 11.33 years and the internal rate of return increases by 5.20%under a typical application scenario.Regarding the effect of different application scenarios in terms of economics,investing in the proposed system is more appropriate in regions with multiple energy demands,especially heating demand.These results demonstrate the technical advantages of the proposed system and provide guiding principles for its design,operation,and project investment.展开更多
The supercritical CO_(2) Brayton cycle is considered a promising energy conversion system for Generation IV reactors for its simple layout,compact structure,and high cycle efficiency.Mathematical models of four Brayto...The supercritical CO_(2) Brayton cycle is considered a promising energy conversion system for Generation IV reactors for its simple layout,compact structure,and high cycle efficiency.Mathematical models of four Brayton cycle layouts are developed in this study for different reactors to reduce the cost and increase the thermohydraulic performance of nuclear power generation to promote the commercialization of nuclear energy.Parametric analysis,multi-objective optimizations,and four decision-making methods are applied to obtain each Brayton scheme’s optimal thermohydraulic and economic indexes.Results show that for the same design thermal power scale of reactors,the higher the core’s exit temperature,the better the Brayton cycle’s thermo-economic performance.Among the four-cycle layouts,the recompression cycle(RC)has the best overall performance,followed by the simple recuperation cycle(SR)and the intercooling cycle(IC),and the worst is the reheating cycle(RH).However,RH has the lowest total cost of investment(C_(tot))of$1619.85 million,and IC has the lowest levelized cost of energy(LCOE)of 0.012$/(kWh).The nuclear Brayton cycle system’s overall performance has been improved due to optimization.The performance of the molten salt reactor combined with the intercooling cycle(MSR-IC)scheme has the greatest improvement,with the net output power(W_(net)),thermal efficiencyη_(t),and exergy efficiency(η_(e))improved by 8.58%,8.58%,and 11.21%,respectively.The performance of the lead-cooled fast reactor combined with the simple recuperation cycle scheme was optimized to increase C_(tot) by 27.78%.In comparison,the internal rate of return(IRR)increased by only 7.8%,which is not friendly to investors with limited funds.For the nuclear Brayton cycle,the molten salt reactor combined with the recompression cycle scheme should receive priority,and the gas-cooled fast reactor combined with the reheating cycle scheme should be considered carefully.展开更多
Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal mus...Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal muscular atrophy-like clinical phenotype.The aims of this study were to determine the mechanism of the severe phenotype caused by the MORC2 p.S87L mutation and to explore potential treatment strategies.Epithelial cells were isolated from urine samples from a spinal muscular atrophy(SMA)-like patient[MORC2 p.S87L),a CMT2Z patient[MORC2 p.Q400R),and a healthy control and induced to generate pluripotent stem cells,which were then differentiated into motor neuron precursor cells.Next-generation RNA sequencing followed by KEGG pathway enrichment analysis revealed that differentially expressed genes involved in the PI3K/Akt and MAP K/ERK signaling pathways were enriched in the p.S87L SMA-like patient group and were significantly downregulated in induced pluripotent stem cells.Reduced proliferation was observed in the induced pluripotent stem cells and motor neuron precursor cells derived from the p.S87L SMA-like patient group compared with the CMT2Z patient group and the healthy control.G0/G1 phase cell cycle arrest was observed in induced pluripotent stem cells derived from the p.S87L SMA-like patient.MORC2 p.S87Lspecific antisense oligonucleotides(p.S87L-ASO-targeting)showed significant efficacy in improving cell prolife ration and activating the PI3K/Akt and MAP K/ERK pathways in induced pluripotent stem cells.Howeve r,p.S87L-ASO-ta rgeting did not rescue prolife ration of motor neuron precursor cells.These findings suggest that downregulation of the PI3K/Akt and MAP K/ERK signaling pathways leading to reduced cell proliferation and G0/G1 phase cell cycle arrest in induced pluripotent stem cells might be the underlying mechanism of the severe p.S87L SMA-like phenotype.p.S87L-ASO-targeting treatment can alleviate disordered cell proliferation in the early stage of pluripotent stem cell induction.展开更多
Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and th...Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and the AsA–GSH cycle under drought stress in wheat has not been studied.In this study,a hydroponic experiment was conducted in wheat seedlings subjected to 15%polyethylene glycol(PEG)6000–induced dehydration.Drought stress caused the rapid accumulation of endogenous ABA and H_(2)O_(2) and significantly decreased the number of root tips compared with the control.The application of ABA significantly increased the number of root tips,whereas the application of H_(2)O_(2) markedly reduced the number of root tips,compared with that under 15%PEG-6000.In addition,drought stress markedly increased the DHA,GSH and GSSG levels,but decreased the AsA levels,AsA/DHA and GSH/GSSG ratios compared with those in the control.The activities of the four enzymes in the AsA–GSH cycle were also markedly increased under drought stress,including glutathione reductase(GR),ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate reductase(DHAR),compared with those in the control.However,the application of an ABA inhibitor significantly inhibited GR,DHAR and APX activities,whereas the application of an H_(2)O_(2) inhibitor significantly inhibited DHAR and MDHAR activities.Furthermore,the application of ABA inhibitor significantly promoted the increases of H_(2)O_(2) and the application of H_(2)O_(2) inhibitor significantly blocked the increases of ABA,compared with those under 15% PEG-6000.Taken together,the results indicated that ABA and H_(2)O_(2) probably interact under drought stress in wheat;and both of them can mediate drought stress by modulating the enzymes in AsA–GSH cycle,where ABA acts as the main regulator of GR,DHAR,and APX activities,and H_(2)O_(2) acts as the main regulator of DHAR and MDHAR activities.展开更多
基金funded by the National Natural Science Foundation of China(grant Nos.22322803,22178059,22208054,and 22221005)Key Technologies Innovation and Industrialization Projects of Fujian Province(grant No.2022G031)Qingyuan Innovation Laboratory(grant Nos.00121002 and 00523005)
文摘The development of sustainable techniques to produce high-performance zeolite is essential to achieve green production in industry.Herein,we report an eco-friendly route to synthesizing hierarchical Beta zeolite from kaolinite and recycled mother liquor.The results reveal that the unutilized species(such as silicon species and Na+)in mother liquor stayed in a stable concentration during eleven recycled experiments.Moreover,the synthesized Beta zeolites still have comparable physicochemical properties and catalytic performance in the esterification of levulinic acid with ethanol over the initial zeolite although eleven recycled experiments.Life cycle assessment exhibits that the synthesis of Beta zeolite with recycled mother liquor can reduce global warming potential by 23%and resource depletion-water use by 36%compared to that without recycled mother liquor.This quantitatively demonstrates that the approach proposed in this work is really a sustainable one,extremely increasing the utilization efficiency of raw materials and decreasing the environmental burden.
基金financially sponsored by Qing Lan Project in Jiangsu Province of China(2023)Scientific Research Project of Taizhou Polytechnic College(TZYKY-22-4).
文摘The reuse of waste recycled concrete from harsh environments has become a research hotspot in the field of construction.This study investigated the repair effect of carbonation treatment on second-generation recycled fine aggregate(SRFA)obtained from recycled fine aggregate concrete(RFAC)subjected to freeze-thaw(FT)cycles.Before and after carbonation,the properties of SRFA were evaluated.Carbonated second-generation recycled fine aggregate(CSRFA)at five substitution rates(0%,25%,50%,75%,100%)to replace SRFA was used to prepare carbonated second-generation recycled fine aggregate concrete(CSRFAC).The water absorption,porosity and mechanical properties of CSRFAC were tested,and its frost-resisting durability was evaluated.The results showed after carbonation treatment,the physical properties of SRFA was improved and met the requirements of II aggregate.The micro-hardness of the interfacial transition zone and attached mortar in CSRFA was 50.5%and 31.2%higher than that in SRFA,respectively.With the increase of CSRFA replacement rate,the water absorption and porosity of CSRFAC gradually decreased,and the mechanical properties and frost resistance of CSRFAC were gradually improved.Carbonation treatment effectively repairs the damage of SRFA caused by FT cycles and improves its application potential.
基金This research was funded by the National Natural Science Foundation of China(52078068)Practice Innovation Program of Jiangsu Province(KYCX22_3082).
文摘With the emphasis on environmental issues,the recycling of waste concrete,even recycled concrete,has become a hot spot in the field of architecture.But the repeated recycling of waste concrete used in harsh environments is still a complex problem.This paper discusses the durability and recyclability of recycled aggregate concrete(RAC)as a prefabricated material in the harsh environment,the effect of high-temperature curing(60℃,80℃,and 100℃)on the frost resistance of RAC and physical properties of the second generation recycled coarse aggregate(RCA_(2))of RAC after 300 freeze-thaw cycles were studied.The frost resistance of RAC was characterized by compressive strength,relative dynamic elastic modulus,and mass loss.As the physical properties of RCA_(2),the apparent density,water absorption,and crushing value were measured.And the SEM images of RAC after 300 freeze-thaw cycles were shown.The results indicated that the frost resistance of RAC cured at 80℃ for 7 days was comparable to that cured in the standard condition(cured for 28 days at 20℃±2℃ and 95%humidity),and the RAC cured at 100℃ was slightly worse.However,the frost resistance of RAC cured at 60℃ deteriorated seriously.The RAC cured at 80℃ for 7 days is the best.Whether after the freeze-thaw cycle or not,the RCA that curd at 60℃,80℃,and 100℃ for 7 days can also meet the requirements of Grade III RCA and be used as the aggregate of non-bearing part of prefabricated concrete components.RCA_(2) which is cured at 80℃ for 7 days had the best physical properties.
基金Supported by the Special Funds for Major State Basic Research Project of China(No.2009CB220104)the Science and Technology Bureau of Shenyang, China(Nos.108149-2-00, 1091242-6-00)
文摘1 Introduction Low-dimensional nanostructures, such as nanorods, nanowires, and nanotubes, have received much attention for their superior optical, electrical, catalytic and magnetic properties. Owing to their low dimensionality and quantum continement effect, low-dimensional nanoscale materials can be exploited as fundamental building blocks for nanoscience and nanodevices^[1-3]. In recent years, efforts have been devoted to develop new approaches to synthesize one-dimensional(lD) nanostructrued vanadium oxides or vanadates materials, such as V205, NaV2Os, and CuV206, which have been widely investigated in catalytic or electrochemical fields due to their outstanding structural flexibility^[4-6].
基金The National Natural Science Foundation of China(No.51578141)the Major State Basic Research Development Program of China(No.2015CB655102)China-Japanese Research Cooperative Program-Ministry of Science and Technology in China(No.2016YFE0118200)
文摘The degradation progress of recycled aggregate concrete(RAC) subjected to sulfate attack under wetting-drying cycles and flexural loading is studied. Three different stress ratios(0, 0.3 and 0.5) were applied in this test. The variations of relative dynamic elastic modulus Erd and water-soluble SO2-4 contents in RAC were used to evaluate degradation progress. The changes in mineral products and microstructures of interior concrete were investigated by means of X-ray diffraction(XRD), the environmental scanning electron microscope(ESEM) and X-ray computed tomography(X-CT). The results indicate that flexural loading accelerates the degradation of RAC under sulfate attack and wetting-drying cycles by expediting the transmission of SO2-4 into interior concrete. Furthermore, the accelerated effect of flexural loading is more obvious with the increase of stress ratio, that is because higher stress ratios can accelerate the extension of microcracks and generate more channels for the transmission of SO2-4. Also, more expansive products(gypsum and ettringite) are generated by the reaction of Ca(OH)2 with SO2-4, which can further accelerate the degradation of RAC.
文摘This work examines the environmental and geochemical impact of recycled aggregate concrete production with properties representative for structural applications.The environmental influence of cement content,aggregate production,transportation,and waste landfilling is analysed by undertaking a life cycle assessment and considering a life cycle inventory largely specific for the region.To obtain a detailed insight into the optimum life cycle parameters,a sensitivity study is carried out in which supplementary cementitious materials,different values of natural-to-recycled aggregate content ratio and case-specific transportation distances were considered.The results show that carbon emissions were between 323 and 332 kgCO_(2)e per cubic metre of cement only natural aggregate concrete.These values can be reduced by up to 17%by replacing 25%of the cement with fly ash.By contrast,carbon emissions can increase when natural coarse aggregates are replaced by recycled aggregates in proportions of 50%and 100%,and transportation is not included in analysis.However,the concrete with 50%recycled aggregate presented lower increase,only 0.3%and 3.4%for normal and high strength concrete,respectively.In some cases,the relative contribution of transportation to the total carbon emissions increased when cement was replaced by fly ash in proportions of 25%,and case-specific transportation distances were considered.In absolute values,the concrete mixes with 100%recycled aggregates and 25%fly ash had lower carbon emissions than concrete with cement and natural aggregates only.Higher environmental benefits can be obtained when the transportation distances of fly ash are relatively short(15–25 km)and the cement replacement by fly ash is equal or higher than 25%,considering that the mechanical properties are adequate for practical application.The observations from this paper show that recycled aggregate concrete with strength characteristics representative for structural members can have lower carbon emissions than conventional concrete,recommending them as an alternative to achieving global sustainability standards in construction.
基金supported by the TPF-5 (129) Recycled Unbound Materials Pool Fund administered by the Minnesota Department of Transportation and the Recycled Materials Resource Center (RMRC)supported by the U.S. Federal Highway Administration
文摘The objective of this project was to characterize the freeze-thaw properties of recycled concrete(RCA)and asphalt(RAP)as unbound base and to assess how they behaved in the field for nearly 8 years.This paper includes an examination of existing information,laboratory studies of freeze-thaw behavior,and evaluation of data from Mn ROAD field-test sections in a seasonally cold region,i.e.,in Minnesota,USA.Test sections were constructed using recycled materials in the granular base layers at the Mn ROAD test facility.One test section included 100%RAP,another 100%RCA,a third one a 50/50blend of RCA/natural aggregate,and a fourth one only natural aggregate(Class 5)as a control.The stiffness(i.e.,elastic modulus)was monitored during construction and throughout the pavement life by the Minnesota Department of Transportation,along with the variation of temperatures and moisture regimes in the pavement to determine their effects on pavement performance.The resilient modulus of each material was determined by bench-scale testing in accordance with NCHRP 1-28a,as well as by field-scale tests incorporating a falling-weight deflectometer.Specimens were subjected to as many as 20 cycles of freeze-thaw in the laboratory,and the change in their resilient modulus was measured.In the field-test sections constructed with the same materials as the base course,temperature,moisture,and field modulus(from fallingweight deflectometer tests)were monitored seasonally for nearly 8 years.From the temperatures in the base course layer,the number of freeze-thaw cycles experienced in the field was determined for each test section.Inferences were made relative to modulus change versus freeze-thaw cycles.Conclusions were drawn for long-term field performances of the recycled base(RAB)in comparison to natural aggregate.
文摘The paper studied the relationship between microstructure and shape recovery characteristics by using colored microstructure analysis under polarized light on the thermomechanical cycled CuAlNi single crystals. The two-way shape memory effect in quenched thin bar resulted from the preferential formation/extinction of martensite variant due to the internal quench stress, and the variant was formed at an angle of about 45 deg. with the tension direction ([001] of the βphase). Initial thermomechanical cycling under relatively low stress single variant stress-induced martensite was formed at an angle of 45 deg. with the tension and its morphology was a lath of parallel twins. More than one group of variants were formed after several training cycles and such variants also caused tilting of some thermally formed accommodated martensite. By overheating the trained sample containing stabilized multi-variants of stress-induced martensite, very coarse martensite structure with a strong asymmetry was produced, which caused the reverse two-way shape memory effect.
基金supported by the National Natural Science Foundation of China (Nos. 50762004 and 50864004)
文摘A new LiCoO2 recovery technology for Li-ion batteries was studied in this paper. LiCoO2 was peeled from the Al foil with dimethyl acetamide (DMAC), and then polyvinylidene fluoride (PVDF) and carbon powders in the active material were eliminated by high temperature calcining. Subsequently, Li2CO3, LiOH-H20 and LiAc-2H2O were added into the recycled powders to adjust the Li/Co molar ratio to 1.00. The new LiCoO2 was obtained by calcining the mixture at 850℃ for 12 h in air. The structure and morphology of the recycled powders and resulting samples were studied by XRD and SEM techniques, respectively. The layered structure of LiCoO2 synthesized by adding Li2CO3 is the best, and it is found to have the best characteristics as a cathode material in terms of charge-discharge capacity and cycling performance. The first discharge capacity is 160 mAh·g^-1 between 3.0-4.3 V. The discharge capacity after cycling for 50 times is still 145.2 mAh·g^-1.
文摘Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular components in the circulating water. The objective of the simulation is to determine the maximum allowable concentrations of ions and molecules as well as the choice of conditions for deposition or adsorption.First of all, our examinations decrease the concentration of copper ions and fatty acids in the circulating water. By pre-mixing water with the highest concentration of these ions, a reduction of copper ion and fatty acid concentrations in the recycled water occurs. The results do not only ensure the achievement of the maximum permitted concentration(MPC) of copper and iron, significantly reducing the amount of oxidized copper, they also make it possible to use the united sewage as current water for the flotation process. Mixing and adding filtrate of tailings, discharges of urban wastewater treatment and effluent of ash pit of thermal power stations(TPS) to recycled water causes an increase in the capacity of the enrichment plant by 15–17%.
文摘The mechanical properties of polyvinyl alcohol (PVA) films prepared by evaporating water from freeze/thaw cycled gel were investigated as a function of the number of freeze/thaw cycles. The maximum stress of the PVA film prepared by freeze/thaw cycling was larger than that prepared without the freeze/thaw cycle process. The largest maximum stress was 46.2 MPa for a film prepared with 10 freeze/thaw cycles, which was twice as large as that for a cast PVA film without freeze/thaw cycling (22.3 MPa). This is due to the formation of small crystallites during the freeze/thaw cycle process. Furthermore, when the film was annealed at 130°C, the maximum stress was as high as 181 MPa which was comparable to that for PVA films prepared using additives. The crystallinity is not the main factor that determines the maximum stress for either the non-annealed or annealed freeze/thaw cycled films, but the glass transition temperature is well correlated with the maximum stress, irrespective of the annealing process. This is due to the different molecular morphology;the non-annealed freeze/thaw cycled film consists of many small crystallites, but the annealed film consists of larger crystallites formed during the annealing process.
文摘Wake-up radio (WuR) system is often presented as the best candidate for replacing traditional duty cycled Medium Access Control (MAC) protocols in Wireless Sensor Networks (WSNs). The Double Radio (DoRa) protocol is a new MAC protocol for in-band WuR system with addressing capabilities. While the DoRa protocol improves the WSNs energy efficiency, it still suffers from an overhearing problem when the WuR system is very often requested. The WuR wastes a noticeable amount of energy when overhearing to wake-up demand intended to other nodes, but it is neither measured nor solved in other works. In this paper, an adaptive duty-cycled DoRa (DC-DoRa) is then proposed to solve the overhearing problem. The primary concept of the work is to enable the WuR functionality before the node is addressed and to disable the WuR after the node sent data. Extensive simulations under OMNeT++ using real input parameters are then performed to show the significant energy-savings through the two protocols and the nearly suppression of overhearing with DC-DoRa. In fact, the mean power consumption is three-order below using the DoRa protocol compared to traditional MAC protocols. While overhearing can represent up to 93% of the WuR energy consumption with the DoRa protocol, it is reduced to only 1% with the DC-DoRa protocol.
基金supported by the National Natural Science Foundation of China(Grant Nos.52175188,and 52201087)the Key Research and Development Program of Shaanxi Province(Grant No.2023-YBGY-434)+3 种基金the Natural Science Foundation of Shaanxi Province(Grant No.2022JM-253)the Open Fund of Liaoning Provincial Key Laboratory of Aero-engine Materials Tribology(Grant No.LKLAMTF202301)the Science and Technology on Reactor System Design Technology Laboratorythe Fundamental Research Funds for the Central Universities。
文摘Optimized macroscopic tribological behavior can be anticipated in metallic glasses(MGs)by cryogenic cycling treatment(CCT),which is attributed to enhanced plasticity.However,the intrinsic friction mechanisms of MGs induced by cryogenic rejuvenation are still poorly understood.In the present study,nanoscopic wear tests were conducted on the Zr-based MGs surface with different CCT cycles using atomic force microscopy(AFM).After CCT treatment with 100 cycles,the MG displays the highest adhesion and ploughing frictions,but significantly improved anti-wear properties.Adhesion tests and molecular dynamics simulations disclose that the increased adhesion is attributed to the dominance of liquid-like regions in the CCT-treated MGs,and the impact of reduced hardness and weak elastic recovery results in the deteriorated ploughing friction.The enhanced plasticity effectively dissipates the strain from the AFM tip through multiple shear bands and weakens the adhesion during deformation,giving rise to excellent wear resistance.This study elucidates the promoting effect of CCT on the outstanding antiwear performance of MGs,and is helpful for the development of novel alloys.
基金National Natural Science Foundation of China,Grant/Award Numbers:22109023,22179022,22209027Industry-University-Research Joint Innovation Project of Fujian Province,Grant/Award Number:2021H6006+2 种基金FuXiaQuan National Independent Innovation Demonstration Zone Collaborative Innovation Platform,Grant/Award Number:2022-P-027Youth Innovation Fund of Fujian Province,Grant/Award Numbers:2021J05043,2022J05046Science and Technology。
文摘The indiscriminate utilization of nondegradable polyethylene terephthalate(PET)-based products has triggered serious environmental pollution that has to be resolved vigorously.A simple synthesis of N-doped carbon nanotubes from recycled PET(NCNTs_(r-PET))was developed by a nitric acid-assisted hydrothermal method.Experimental results and theoretical calculations show that the intrinsic defects in CNTs_(r-PET)would induce N-doping by NH_(3)generated from nitric acid during the hydrothermal process,thus producing the NCNTs_(r-PET).The life cycle assessment proves that the developed method for N-doped CNTs using r-PET as the carbon source is more environmentally friendly than the conventional chemical vapor deposition using acetylene as the carbon source.As a typical application,the NCNTs_(r-PET)delivered an impressive sodium storage capacity with an ultralong lifespan.This work not only provides a new route to upcycling waste plastics into valuable carbonaceous materials in an ecofriendly manner,but also reveals a basic understanding of the N-doping mechanism in carbonaceous materials.
基金The authors gratefully acknowledge the financial support of National NaturalScience Foundation of China(Grant No.41972276)Natural Science Foundation of Fujian Province,China(Grant No.2020J06013)"Foal Eagle Program"Youth Top-notch Talent Project of Fujian Province,China(Grant No.00387088).
文摘Enzyme-induced carbonate precipitation(EICP)is an emanating,eco-friendly and potentially sound technique that has presented promise in various geotechnical applications.However,the durability and microscopic characteristics of EICP-treated specimens against the impact of drying-wetting(D-W)cycles is under-explored yet.This study investigates the evolution of mechanical behavior and pore charac-teristics of EICP-treated sea sand subjected to D-W cycles.The uniaxial compressive strength(UCS)tests,synchrotron radiation micro-computed tomography(micro-CT),and three-dimensional(3D)recon-struction of CT images were performed to study the multiscale evolution characteristics of EICP-reinforced sea sand under the effect of D-W cycles.The potential correlations between microstructure characteristics and macro-mechanical property deterioration were investigated using gray relational analysis(GRA).Results showed that the UCS of EICP-treated specimens decreases by 63.7% after 15 D-W cycles.The proportion of mesopores gradually decreases whereas the proportion of macropores in-creases due to the exfoliated calcium carbonate with increasing number of D-W cycles.The micro-structure in EICP-reinforced sea sand was gradually disintegrated,resulting in increasing pore size and development of pore shape from ellipsoidal to columnar and branched.The gray relational degree suggested that the weight loss rate and UCS deterioration were attributed to the development of branched pores with a size of 100-1000 m m under the action of D-W cycles.Overall,the results in this study provide a useful guidancee for the long-term stability and evolution characteristics of EICP-reinforced sea sand under D-W weathering conditions.
基金This work was supported by the Chinese TMSR Strategic Pioneer Science and Technology Project(No.XDA02010300).
文摘A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the molten salt reactor and power system.This model considers the impact of thermal properties of fluid variation on accuracy and has been validated with Simulink.This study reveals the capability of the control system to compensate for anomalous situations and maintain shaft stability in the event of perturbations occurring in high-temperature molten salt tank outlet parameters.Meanwhile,the control system’s impact on the system’s dynamic characteristics under molten salt disturbance is also analyzed.The results reveal that after the disturbance occurs,the controlled system benefits from the action of the control,and the overshoot and disturbance amplitude are positively correlated,while the system power and frequency eventually return to the initial values.This simulation model provides a basis for utilizing molten salt reactors for power generation and maintaining grid stability.
文摘To meet the goal of worldwide decarbonization,the transformation process toward clean and green energy structures has accelerated.In this context,coal-fired power plant(CFPP)and large-scale energy storage represented by compressed air energy storage(CAES)technology,are tasked with increasing renewable resource accommodation and maintaining the power system security.To achieve this,this paper proposes the concept of a CFPP-CAES combined cycle and a trigenerative system based on that.Considering the working conditions of the CFPP,thermal characteristics of three typical operation modes were studied and some general regularities were identified.The results of various potential integration schemes discussion indicated that extracting water from low-temperature points in the feedwater system to cool pressurized air and simultaneously increase the backwater temperature is beneficial for improving performance.In addition,preheating the pressurized air before the air expanders via lowgrade water in the feedwater system as much as possible and reducing extracted steam contribute to increasing the efficiency.With the optimal integration scheme,2.85 tonnes of coal can be saved per cycle and the round-trip efficiency can be increased by 2.24%.Through the cogeneration of heat and power,the system efficiency can reach 77.5%.In addition,the contribution degree of the three compression heat utilization methods to the performance improvement ranked from high to low,is preheating the feedwater before the boiler,supplying heat,and flowing into the CFPP feedwater system.In the cooling energy generation mode,the system efficiency can be increased to over 69%.Regardless of the operation mode,the benefit produced by integration is further enhanced when the CFPP operates at higher operating conditions because the coupling points parameters are changed.In addition,the dynamic payback period can be shortened by 11.33 years and the internal rate of return increases by 5.20%under a typical application scenario.Regarding the effect of different application scenarios in terms of economics,investing in the proposed system is more appropriate in regions with multiple energy demands,especially heating demand.These results demonstrate the technical advantages of the proposed system and provide guiding principles for its design,operation,and project investment.
基金This work was supported of National Natural Science Foundation of China Fund(No.52306033)State Key Laboratory of Engines Fund(No.SKLE-K2022-07)the Jiangxi Provincial Postgraduate Innovation Special Fund(No.YC2022-s513).
文摘The supercritical CO_(2) Brayton cycle is considered a promising energy conversion system for Generation IV reactors for its simple layout,compact structure,and high cycle efficiency.Mathematical models of four Brayton cycle layouts are developed in this study for different reactors to reduce the cost and increase the thermohydraulic performance of nuclear power generation to promote the commercialization of nuclear energy.Parametric analysis,multi-objective optimizations,and four decision-making methods are applied to obtain each Brayton scheme’s optimal thermohydraulic and economic indexes.Results show that for the same design thermal power scale of reactors,the higher the core’s exit temperature,the better the Brayton cycle’s thermo-economic performance.Among the four-cycle layouts,the recompression cycle(RC)has the best overall performance,followed by the simple recuperation cycle(SR)and the intercooling cycle(IC),and the worst is the reheating cycle(RH).However,RH has the lowest total cost of investment(C_(tot))of$1619.85 million,and IC has the lowest levelized cost of energy(LCOE)of 0.012$/(kWh).The nuclear Brayton cycle system’s overall performance has been improved due to optimization.The performance of the molten salt reactor combined with the intercooling cycle(MSR-IC)scheme has the greatest improvement,with the net output power(W_(net)),thermal efficiencyη_(t),and exergy efficiency(η_(e))improved by 8.58%,8.58%,and 11.21%,respectively.The performance of the lead-cooled fast reactor combined with the simple recuperation cycle scheme was optimized to increase C_(tot) by 27.78%.In comparison,the internal rate of return(IRR)increased by only 7.8%,which is not friendly to investors with limited funds.For the nuclear Brayton cycle,the molten salt reactor combined with the recompression cycle scheme should receive priority,and the gas-cooled fast reactor combined with the reheating cycle scheme should be considered carefully.
基金supported by the National Natural Science Foundation of China,Nos.82171172(to RZ)and 81771366(to RZ)Fundamental Research Funds for the Central Universities of Central South University,Nos.2021zzts1095(to SZ)and 2022zzts0832(to HY)。
文摘Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal muscular atrophy-like clinical phenotype.The aims of this study were to determine the mechanism of the severe phenotype caused by the MORC2 p.S87L mutation and to explore potential treatment strategies.Epithelial cells were isolated from urine samples from a spinal muscular atrophy(SMA)-like patient[MORC2 p.S87L),a CMT2Z patient[MORC2 p.Q400R),and a healthy control and induced to generate pluripotent stem cells,which were then differentiated into motor neuron precursor cells.Next-generation RNA sequencing followed by KEGG pathway enrichment analysis revealed that differentially expressed genes involved in the PI3K/Akt and MAP K/ERK signaling pathways were enriched in the p.S87L SMA-like patient group and were significantly downregulated in induced pluripotent stem cells.Reduced proliferation was observed in the induced pluripotent stem cells and motor neuron precursor cells derived from the p.S87L SMA-like patient group compared with the CMT2Z patient group and the healthy control.G0/G1 phase cell cycle arrest was observed in induced pluripotent stem cells derived from the p.S87L SMA-like patient.MORC2 p.S87Lspecific antisense oligonucleotides(p.S87L-ASO-targeting)showed significant efficacy in improving cell prolife ration and activating the PI3K/Akt and MAP K/ERK pathways in induced pluripotent stem cells.Howeve r,p.S87L-ASO-ta rgeting did not rescue prolife ration of motor neuron precursor cells.These findings suggest that downregulation of the PI3K/Akt and MAP K/ERK signaling pathways leading to reduced cell proliferation and G0/G1 phase cell cycle arrest in induced pluripotent stem cells might be the underlying mechanism of the severe p.S87L SMA-like phenotype.p.S87L-ASO-targeting treatment can alleviate disordered cell proliferation in the early stage of pluripotent stem cell induction.
基金This research was funded by the National Key Research and Development Program of China(2023YFD2301505).
文摘Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and the AsA–GSH cycle under drought stress in wheat has not been studied.In this study,a hydroponic experiment was conducted in wheat seedlings subjected to 15%polyethylene glycol(PEG)6000–induced dehydration.Drought stress caused the rapid accumulation of endogenous ABA and H_(2)O_(2) and significantly decreased the number of root tips compared with the control.The application of ABA significantly increased the number of root tips,whereas the application of H_(2)O_(2) markedly reduced the number of root tips,compared with that under 15%PEG-6000.In addition,drought stress markedly increased the DHA,GSH and GSSG levels,but decreased the AsA levels,AsA/DHA and GSH/GSSG ratios compared with those in the control.The activities of the four enzymes in the AsA–GSH cycle were also markedly increased under drought stress,including glutathione reductase(GR),ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate reductase(DHAR),compared with those in the control.However,the application of an ABA inhibitor significantly inhibited GR,DHAR and APX activities,whereas the application of an H_(2)O_(2) inhibitor significantly inhibited DHAR and MDHAR activities.Furthermore,the application of ABA inhibitor significantly promoted the increases of H_(2)O_(2) and the application of H_(2)O_(2) inhibitor significantly blocked the increases of ABA,compared with those under 15% PEG-6000.Taken together,the results indicated that ABA and H_(2)O_(2) probably interact under drought stress in wheat;and both of them can mediate drought stress by modulating the enzymes in AsA–GSH cycle,where ABA acts as the main regulator of GR,DHAR,and APX activities,and H_(2)O_(2) acts as the main regulator of DHAR and MDHAR activities.