Objective To investigate the Hela cells growth inhibition and apoptosis possible molecular mechanisms. Methods Hela cells were treated with various concentrations （100μmol/L,200μmol/L, 300 μmol/L, 400 μmol/L） of...Objective To investigate the Hela cells growth inhibition and apoptosis possible molecular mechanisms. Methods Hela cells were treated with various concentrations （100μmol/L,200μmol/L, 300 μmol/L, 400 μmol/L） of NS-398 （selective for COX-2 inhibition）. Cell growth was measured by MTT （Thiazolyl blue）. Apoptosis was detected by double staining flow cytometry （FCM）. Levels of PGE： were measured by radioimmunoassay. The expressions of COX-2 protein were also examined by Western blot analysis. Results After treated with different concentrations of NS-398, the growth of Hela cells was suppressed significantly in a dose-and time-dependent manner （P〈0.01）. The NS-398 can induce apoptosis with the apoptosis rates at 8.53%-43.46% by FCM in a dose-dependent manner. The release of PGE2 was reduced in Hela cells with the values of 69.26 ± 2.13, 47.46 ± 2.18, 28.15 ± 1.64 and 17.01 ± 1.12, respectively, there was significant difference compared with control group （83.78 ± 1.11） （P〈0.01）. The NS-398 could inhibit the activity and expression of COX-2 in a dose- dependent manner and down-regulated the expression of COX-2 protein greatly. Conclusion NS-398 could inhibit the proliferation and increase apoptosis in human Hela cells. These effects may be depended on the inhibition of the expression of COX-2 and PGE2 by NS-398.展开更多
基金Hubei Provincial Department of Education Funds (D200511008)
文摘Objective To investigate the Hela cells growth inhibition and apoptosis possible molecular mechanisms. Methods Hela cells were treated with various concentrations （100μmol/L,200μmol/L, 300 μmol/L, 400 μmol/L） of NS-398 （selective for COX-2 inhibition）. Cell growth was measured by MTT （Thiazolyl blue）. Apoptosis was detected by double staining flow cytometry （FCM）. Levels of PGE： were measured by radioimmunoassay. The expressions of COX-2 protein were also examined by Western blot analysis. Results After treated with different concentrations of NS-398, the growth of Hela cells was suppressed significantly in a dose-and time-dependent manner （P〈0.01）. The NS-398 can induce apoptosis with the apoptosis rates at 8.53%-43.46% by FCM in a dose-dependent manner. The release of PGE2 was reduced in Hela cells with the values of 69.26 ± 2.13, 47.46 ± 2.18, 28.15 ± 1.64 and 17.01 ± 1.12, respectively, there was significant difference compared with control group （83.78 ± 1.11） （P〈0.01）. The NS-398 could inhibit the activity and expression of COX-2 in a dose- dependent manner and down-regulated the expression of COX-2 protein greatly. Conclusion NS-398 could inhibit the proliferation and increase apoptosis in human Hela cells. These effects may be depended on the inhibition of the expression of COX-2 and PGE2 by NS-398.
