Effects of Different Fertilization Modes on Growth of Cymbidium sinense ‘Qihei'

[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.

墨兰AP3基因过表达载体构建及遗传转化铁皮石斛

为探讨墨兰(Cymbidium sinense)AP3基因在兰花唇瓣中的功能,基于前期从墨兰转录组中得到的AP3基因序列,通过PCR技术构建墨兰AP3基因过表达载体,利用农杆菌EHA105转化铁皮石斛(Dendrobium officinale)原球茎,建立遗传转化体系。经PCR验证,20棵抗性苗中获得9棵阳性苗,其阳性苗率达45%,成功将墨兰AP3基因导入铁皮石斛。

墨兰品种的观赏性状及聚类分析

为了探究墨兰品种间的观赏性状差异和遗传多样性,本研究以13个品种的墨兰为试验材料,用SPSS软件对墨兰品种的8个性状进行系统的聚类分析。结果表明:13个墨兰品种中,‘万代福’、‘江南白墨’和‘企黑爪’植株壮硕、叶片厚实,在花枝等方面具有较高的观赏价值;‘吴字翠’、‘企黑爪’和‘江南白墨’在开花性状上表现最佳。SPSS分析将13个供试品种分成3个群集,类群Ⅰ:‘阳明锦’、‘招财进宝’、‘瑞玉’、‘金鸟’、‘大勋冠’和‘大勋爪’;类群Ⅱ:‘新娘’和‘玉妃’;类群Ⅲ:‘企黑爪’、‘万代福’、‘金华山’、‘吴字翠’和‘江南白墨’,其中各个性状表现最好的是类群Ⅲ,‘企黑爪’、‘万代福’、‘金华山’、‘吴字翠’和‘江南白墨’在8个观赏性状中都表现最佳,这与差异分析结果相一致。本研究发现墨兰品种间的遗传距离较远,其遗传基础广阔,这为今后墨兰杂交种的选育和遗传多样性研究提供了科学依据。