The random amplified polymorphic DNA （RAPD） marker was assessed to detect the genetic relationships among 48 hybrid Cymbidium cultivars from Japan, Korea, China, and USA, and 2 species of native Cymbidium. Twenty pr...The random amplified polymorphic DNA （RAPD） marker was assessed to detect the genetic relationships among 48 hybrid Cymbidium cultivars from Japan, Korea, China, and USA, and 2 species of native Cymbidium. Twenty primers were screened from 100 random decamer primers, and a total of 258 DNA bands were amplified, 253 of which （98.1%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.6. All cultivars were distinguishable when a number of primers were considered. Genetic similarities among the cultivars and species were estimated based on the amount of band sharing ranging from 0.364-0.817 with an average of 0.581. According to the data, a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and native species were classified into five cluster groups with the similarity coefficient of 0.592. It revealed that the genetic relationships among tested accessions were to some extent related with their origin, flower colour, branch type, and genealogy. It further indicated that the RAPD technique is a useful tool for studying the genetic relationships among hybrid Cymbidium cultivars.展开更多
[ Objective] The paper was to study the genetic background of four unique types of fragrance pigs in China. [ Method ] Using 27 pairs of micmsatellite loci jointly recommended by Food and Agricultural Organization （...[ Objective] The paper was to study the genetic background of four unique types of fragrance pigs in China. [ Method ] Using 27 pairs of micmsatellite loci jointly recommended by Food and Agricultural Organization （FAO） and International Society for Animal Genetics （ISAG）, we detected the genotypes of 200 fragrance individuals belonging to four types （Jiuyang fragrance pig, Jianbai fragrance pig, Cangjiang fragrance pig and Huangjiang fragrance pig）, and analyzed their inter-and intra-breed genetic variations. Clustering analysis was also conducted using neighbor-joining （NJ） method and unweighted pair-group method with arithmetic mean （UPGMA）. [ Result] The genetic relationship between Congiiang fragrance pig and Huangjiang fragrance pig was the closest, followed by the ge- netic relationship between Jiuyang fragrance pig and Jianbai fragrance pig; the genetic distance between Jianbai fragrance pig and Huangjiang fragrance pig was the farthest, which was consistent with their geographic distribution, ecological environment and body physical characteristics. Clustering resuhs showed that Jiuyang fragrance pig and Jianbai fragrance pig clustered into one class, while Congjiang fragrance pig and Huangjiang fragrance pig clustered into another class. [ Conclu- sion] The study laid the foundation for quantitative trait loci positioning of good genes of these breeds and implementation of genetic marker-assisted selection.展开更多
文摘The random amplified polymorphic DNA （RAPD） marker was assessed to detect the genetic relationships among 48 hybrid Cymbidium cultivars from Japan, Korea, China, and USA, and 2 species of native Cymbidium. Twenty primers were screened from 100 random decamer primers, and a total of 258 DNA bands were amplified, 253 of which （98.1%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.6. All cultivars were distinguishable when a number of primers were considered. Genetic similarities among the cultivars and species were estimated based on the amount of band sharing ranging from 0.364-0.817 with an average of 0.581. According to the data, a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and native species were classified into five cluster groups with the similarity coefficient of 0.592. It revealed that the genetic relationships among tested accessions were to some extent related with their origin, flower colour, branch type, and genealogy. It further indicated that the RAPD technique is a useful tool for studying the genetic relationships among hybrid Cymbidium cultivars.
文摘[ Objective] The paper was to study the genetic background of four unique types of fragrance pigs in China. [ Method ] Using 27 pairs of micmsatellite loci jointly recommended by Food and Agricultural Organization （FAO） and International Society for Animal Genetics （ISAG）, we detected the genotypes of 200 fragrance individuals belonging to four types （Jiuyang fragrance pig, Jianbai fragrance pig, Cangjiang fragrance pig and Huangjiang fragrance pig）, and analyzed their inter-and intra-breed genetic variations. Clustering analysis was also conducted using neighbor-joining （NJ） method and unweighted pair-group method with arithmetic mean （UPGMA）. [ Result] The genetic relationship between Congiiang fragrance pig and Huangjiang fragrance pig was the closest, followed by the ge- netic relationship between Jiuyang fragrance pig and Jianbai fragrance pig; the genetic distance between Jianbai fragrance pig and Huangjiang fragrance pig was the farthest, which was consistent with their geographic distribution, ecological environment and body physical characteristics. Clustering resuhs showed that Jiuyang fragrance pig and Jianbai fragrance pig clustered into one class, while Congjiang fragrance pig and Huangjiang fragrance pig clustered into another class. [ Conclu- sion] The study laid the foundation for quantitative trait loci positioning of good genes of these breeds and implementation of genetic marker-assisted selection.
文摘采用RAPD标记研究了分属于4种红豆杉属(Taxus Linn.)植物的68个单株的遗传多样性,并采用UPGMA方法分析68个单株的遗传关系。结果表明:12条RAPD引物共扩增出109条带,其中多态性条带108条,多态性条带百分率为99.1%;平均每条引物扩增出9.1条带。南方红豆杉〔T.wallichiana var.mairei(Lemée et Lévl)L.K.Fu et Nan Li.〕种内的多态性条带百分率和观察等位基因数均最高;欧洲红豆杉(T.baccata Linn.)种内的有效等位基因数、Nei’s基因多样度和Shannon’s信息指数均最高;须弥红豆杉(T.wallichiana Zucc.)种内的各项遗传多样性指数均最低。供试4种植物的种内遗传多样度、种间遗传多样度、基因流和种间遗传分化系数分别为0.174 5、0.358 6、0.401 7和0.554 5,表明55.45%的遗传变异发生在种间。南方红豆杉和须弥红豆杉遗传距离最近;曼地亚红豆杉(Taxus×media Rehd.)和须弥红豆杉的遗传距离最远。通过聚类分析可将68个单株分为3组,欧洲红豆杉的18个单株和曼地亚红豆杉的18个单株分别各自聚为1组;须弥红豆杉的16个单株和南方红豆杉的16个单株聚为1组,其中须弥红豆杉的16个单株和南方红豆杉的16个单株又各自聚为1个亚组,且南方红豆杉的雌、雄单株也分别聚在同一分支上,表明须弥红豆杉和南方红豆杉遗传关系较近,而欧洲红豆杉与其他3种植物的遗传关系较远。
