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白前及其混伪品的ITS2分子鉴定 被引量:6
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作者 林好 陈镜安 +2 位作者 李斯璐 豆蒙蒙 刘镛 《中药材》 CAS 北大核心 2017年第11期2531-2536,共6页
目的:采用ITS2序列鉴定白前及其易混伪品,为准确鉴别白前和保证临床用药的安全性提供参考。方法:白前与其混伪品的ITS2序列从Gen Bank数据库中获取,然后使用MEGA 6.06软件进行种内、种间序列变异分析、构建邻接系统树(NJ树)并计算遗传距... 目的:采用ITS2序列鉴定白前及其易混伪品,为准确鉴别白前和保证临床用药的安全性提供参考。方法:白前与其混伪品的ITS2序列从Gen Bank数据库中获取,然后使用MEGA 6.06软件进行种内、种间序列变异分析、构建邻接系统树(NJ树)并计算遗传距离,运用ITS2数据库相似性搜索引擎预测ITS2二级结构。结果:白前ITS2序列长度在246~270 bp范围内,种内变异少且种间存在明显差异,与所有混伪品种间遗传距离为0.026~0.309。NJ树显示白前独聚一支,另外白前有明显ITS2二级结构特征,可与其混伪品明显区分。结论:ITS2序列能够有效地区分白前与其混伪品,为白前的用药安全提供技术保障。 展开更多
关键词 白前 DNA条形码 混伪品 ITS2 分子鉴定
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Pharmacognostical study of Cynanchum stauntonii and Cynanchum glaucescens,botanical sources of TCM Baiqian 被引量:1
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作者 Chayanis Sutcharitchan Li-na Wang +3 位作者 Hang Zhang Xiao-die Li Ya-jun Cui Shen Ji 《Chinese Herbal Medicines》 CAS 2018年第4期379-387,共9页
Objective: Cynanchum stauntonii and Cynanchum glaucescens are botanical species of Baiqian(Cynanchi Stauntonii Rhizoma et Radix) in Chinese Pharmacopoeia, in which, however, there are no microscopic identification.... Objective: Cynanchum stauntonii and Cynanchum glaucescens are botanical species of Baiqian(Cynanchi Stauntonii Rhizoma et Radix) in Chinese Pharmacopoeia, in which, however, there are no microscopic identification. Therefore, we provided the morphological and microscopic identification of the crude drug for updating Chinese Pharmacopoeia.Methods: Twelve batches of C. stauntonii and three batches of C. glaucescens and their crude drugs were taxonomically, morphologically, and microscopically examined.Results: Taxonomically, C. stauntonii had narrowly lanceolate leaves with acuminate apex and 5 mm long petiole; Whereas C. glaucescens was oblong-lanceolate or oblong with rounded or acute apex in leaves,and had very short or no petiole. Morphologically, rhizomes of C. stauntonii and C. glaucescens both had hollow pith, but the hollow pith occupied about a half of the rhizome's diameter in C. stauntonii, whereas only a very small proportion of the overall diameter in C. glaucescens. Moreover, microscopic observation showed the difference in the proportion of xylem and in rhizome transverse-sections of the two species along with the difference in the size of the pith. Finally, laticifers and rhizome epidermal secretory cells were present in the powders of C. stauntonii, but absent from C. glaucescens.Conclusion: Based on observation of morphological and microscopic characteristics, the two species can be distinguished by the size of the pith, proportion of xylem of rhizomes, and crude drug powder characters such as laticifers and secretory cells. 展开更多
关键词 Baiqian cynanchum stauntonii decne.) schltr. ex levl. Cynanchurn glaucescens decne.) Hand.-Mazz. microscopic Identification morphological Identification PHARMACOGNOSY
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