Cysteine-rich secretory protein-1 （CRISP-1） is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cyst...Cysteine-rich secretory protein-1 （CRISP-1） is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cysteine residues. In mammals, CRISP-1 inhibits sperm-egg fusion and can suppress sperm capacitation. The molecular mechanism of action of the mammalian CRISP proteins remains unknown, but certain non-mammalian CRISP proteins can block ion channels. In the rat, CRISP-1 comprises two forms referred to as Proteins D and E. Recent work in our laboratory demonstrates that the D form of CRISP-1 associates transiently with the sperm surface, whereas the E form binds tightly. When the spermatozoa are washed, the E form of CRISP-1 persists on the sperm surface after all D form has dissociated. Cross-linking studies demonstrate different protein-protein interaction patterns for D and E, although no binding partners for either protein have yet been identified. Mass spectrometric analyses revealed a potential post-translational modification on the E form that is not present on the D form. This is the only discernable difference between Proteins D and E, and presumably is responsible for the difference in behavior of these two forms of rat CRISP- 1. These studies demonstrate that the more abundant D form interacts with spermatozoa transiently, possibly with a specific receptor on the sperm surface, consistent with a capacitation-suppressing function during sperm transit and storage in the epididymis, and also confirm a tightly bound population of the E form that could act in the female reproductive tract. （Asian J Androl 2007 July; 9： 508-514）展开更多
A series of N1-substituted-3-aryl-4-alkyl-4, 5-dihydro-1H-1-pyrazolethiocarboxamide were prepared from the Mannich bases of aryl ketones in good yields. Some derivatives were found to be active against the cysteine p...A series of N1-substituted-3-aryl-4-alkyl-4, 5-dihydro-1H-1-pyrazolethiocarboxamide were prepared from the Mannich bases of aryl ketones in good yields. Some derivatives were found to be active against the cysteine protease of T.cruzi..展开更多
Objective:To study the preventive and therapeutic effect of N-Acetyl-L-cysteine on infectionassociated preterm labor in mice.Methods:A total of 66 C57BL/6 inbred strain pregnant mice were selected and randomly divided...Objective:To study the preventive and therapeutic effect of N-Acetyl-L-cysteine on infectionassociated preterm labor in mice.Methods:A total of 66 C57BL/6 inbred strain pregnant mice were selected and randomly divided into groups A,B and C,with 22 cases in each group.Group A,B and C were regarded as model group,prevention group and treatment group,respectively.The model of infection-associated preterm labor was built by intraperitoneal injection of Escherichia coli.Ten mice of each group were taken and observed the preterm birth rates and live birth rates,respectively.Three mice of each group were killed at 3 h,6 h,12 h and 24 h after building the model.Their uterus tissues were collected and the expressions of the AP-1 and MCP-1 in those tissues were assayed with immunohistochemical method and the expressions of NF-κ Bp65 and TNF- protein in the placenta tissues of those mice were also detected with immunohistochemical method.Results:The preterm birth rates of mice in groups B and C were significantly lower than that in group A,while their live birth rates were distinctly higher than that in group A(P<0.05);the expressions of the AP-1 and MCP-1 in the uterus tissues and NF-κ Bp65 and TNF- protein in the placenta tissues of mice in groups B and C were evidently lower than those in group A(P<0.05);the comparison of the expressions of the NF-κ Bp65 and TNF- between group B and C showed no statistical differences(P>0.05).Conclusions:N-Acetyl-L-cysteine can lower the incidence rate of infection-associated preterm labor by prohibiting the activation of the protein AP-1/MCP-1and decreasing the expression of NF- κ Bp65 and TNF- in the pregnant tissues of premature mice to reduce the inflammatory reactions.展开更多
文摘Cysteine-rich secretory protein-1 （CRISP-1） is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cysteine residues. In mammals, CRISP-1 inhibits sperm-egg fusion and can suppress sperm capacitation. The molecular mechanism of action of the mammalian CRISP proteins remains unknown, but certain non-mammalian CRISP proteins can block ion channels. In the rat, CRISP-1 comprises two forms referred to as Proteins D and E. Recent work in our laboratory demonstrates that the D form of CRISP-1 associates transiently with the sperm surface, whereas the E form binds tightly. When the spermatozoa are washed, the E form of CRISP-1 persists on the sperm surface after all D form has dissociated. Cross-linking studies demonstrate different protein-protein interaction patterns for D and E, although no binding partners for either protein have yet been identified. Mass spectrometric analyses revealed a potential post-translational modification on the E form that is not present on the D form. This is the only discernable difference between Proteins D and E, and presumably is responsible for the difference in behavior of these two forms of rat CRISP- 1. These studies demonstrate that the more abundant D form interacts with spermatozoa transiently, possibly with a specific receptor on the sperm surface, consistent with a capacitation-suppressing function during sperm transit and storage in the epididymis, and also confirm a tightly bound population of the E form that could act in the female reproductive tract. （Asian J Androl 2007 July; 9： 508-514）
文摘A series of N1-substituted-3-aryl-4-alkyl-4, 5-dihydro-1H-1-pyrazolethiocarboxamide were prepared from the Mannich bases of aryl ketones in good yields. Some derivatives were found to be active against the cysteine protease of T.cruzi..
基金supported by the special plan of maternal and child health molecular genetic medicine of Maternal and Child Health Care Center in Chinese Center for Discase Control and Prevention(Grant No.FY-ZX-ZD-0059)
文摘Objective:To study the preventive and therapeutic effect of N-Acetyl-L-cysteine on infectionassociated preterm labor in mice.Methods:A total of 66 C57BL/6 inbred strain pregnant mice were selected and randomly divided into groups A,B and C,with 22 cases in each group.Group A,B and C were regarded as model group,prevention group and treatment group,respectively.The model of infection-associated preterm labor was built by intraperitoneal injection of Escherichia coli.Ten mice of each group were taken and observed the preterm birth rates and live birth rates,respectively.Three mice of each group were killed at 3 h,6 h,12 h and 24 h after building the model.Their uterus tissues were collected and the expressions of the AP-1 and MCP-1 in those tissues were assayed with immunohistochemical method and the expressions of NF-κ Bp65 and TNF- protein in the placenta tissues of those mice were also detected with immunohistochemical method.Results:The preterm birth rates of mice in groups B and C were significantly lower than that in group A,while their live birth rates were distinctly higher than that in group A(P<0.05);the expressions of the AP-1 and MCP-1 in the uterus tissues and NF-κ Bp65 and TNF- protein in the placenta tissues of mice in groups B and C were evidently lower than those in group A(P<0.05);the comparison of the expressions of the NF-κ Bp65 and TNF- between group B and C showed no statistical differences(P>0.05).Conclusions:N-Acetyl-L-cysteine can lower the incidence rate of infection-associated preterm labor by prohibiting the activation of the protein AP-1/MCP-1and decreasing the expression of NF- κ Bp65 and TNF- in the pregnant tissues of premature mice to reduce the inflammatory reactions.
