Summary： In order to study whether cysteine-rich 61 protein （cyr61） is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone （Dxm） on the expression of cyr61 ...Summary： In order to study whether cysteine-rich 61 protein （cyr61） is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone （Dxm） on the expression of cyr61 in the lung tissues of asthmatic mice was investigated. Forty BALB/c mice were divided into asthma group （n=15）, control group （n=10） and Dxm group （n=15）. The asthma group was sensitized and challenged by ovalbumin （OVA）. The mice in Dxm group were intraperitoneally administered with Dxm after OVA challenge. The expression of cyr61 in the lung tissues was detected by using immuno- histochemistry, and that of eotaxin protein in the bronchoalveolar lavage fluid （BALF） by using en- zyme-linked immunosorbent assay （ELISA）. The number of inflammatory cells in BALF was also ana- lyzed. The results showed that the cyr61 expression was highest in asthma group （P〈0.05）, followed by Dxm group （P〈0.05） and control group. The cyr61 had a positive correlation with the total nucleated cells （r=0.867, P〈0.05）, especially eosinophils （r=0.856, P〈0.05）, and eotaxin level （r=0.983, P〈0.05） in the BALF. Our findings suggested that cyr61 is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils.展开更多
目的:检测细胞基质蛋白Cyr61和CTGF在重度子痫前期患者胎盘组织中的定位及表达,研究它们在子痫前期发病中的作用。方法:采用免疫组化SP法和实时荧光定量聚合酶链式反应(quantitative real time polymerase chain reaction,real-time PCR...目的:检测细胞基质蛋白Cyr61和CTGF在重度子痫前期患者胎盘组织中的定位及表达,研究它们在子痫前期发病中的作用。方法:采用免疫组化SP法和实时荧光定量聚合酶链式反应(quantitative real time polymerase chain reaction,real-time PCR)方法检测胎盘组织中Cyr61和CTGF的表达,其中重度子痫前期患者34例,正常妊娠16例。结果:(1)细胞基质蛋白Cyr61和CTGF在正常妊娠孕妇及重度子痫前期患者胎盘组织中均有表达;(2)Cyr61在重度子痫前期患者胎盘组织的表达水平低于正常妊娠孕妇胎盘(P<0.01);(3)CTGF在重度子痫前期患者胎盘组织的表达水平高于正常妊娠孕妇胎盘(P<0.05);(4)Cyr61和CTGF mRNA在重度子痫前期患者胎盘组织的表达水平呈负相关(P<0.01)。结论:Cyr61表达下调和CTGF过度表达与子痫前期的发病有关,并在该病的发病过程中起到一定的作用。展开更多
基金supported by grants from the National Natural Science Foundation of China (No.81170021 and No.30900647)
文摘Summary： In order to study whether cysteine-rich 61 protein （cyr61） is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone （Dxm） on the expression of cyr61 in the lung tissues of asthmatic mice was investigated. Forty BALB/c mice were divided into asthma group （n=15）, control group （n=10） and Dxm group （n=15）. The asthma group was sensitized and challenged by ovalbumin （OVA）. The mice in Dxm group were intraperitoneally administered with Dxm after OVA challenge. The expression of cyr61 in the lung tissues was detected by using immuno- histochemistry, and that of eotaxin protein in the bronchoalveolar lavage fluid （BALF） by using en- zyme-linked immunosorbent assay （ELISA）. The number of inflammatory cells in BALF was also ana- lyzed. The results showed that the cyr61 expression was highest in asthma group （P〈0.05）, followed by Dxm group （P〈0.05） and control group. The cyr61 had a positive correlation with the total nucleated cells （r=0.867, P〈0.05）, especially eosinophils （r=0.856, P〈0.05）, and eotaxin level （r=0.983, P〈0.05） in the BALF. Our findings suggested that cyr61 is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils.
文摘目的:检测细胞基质蛋白Cyr61和CTGF在重度子痫前期患者胎盘组织中的定位及表达,研究它们在子痫前期发病中的作用。方法:采用免疫组化SP法和实时荧光定量聚合酶链式反应(quantitative real time polymerase chain reaction,real-time PCR)方法检测胎盘组织中Cyr61和CTGF的表达,其中重度子痫前期患者34例,正常妊娠16例。结果:(1)细胞基质蛋白Cyr61和CTGF在正常妊娠孕妇及重度子痫前期患者胎盘组织中均有表达;(2)Cyr61在重度子痫前期患者胎盘组织的表达水平低于正常妊娠孕妇胎盘(P<0.01);(3)CTGF在重度子痫前期患者胎盘组织的表达水平高于正常妊娠孕妇胎盘(P<0.05);(4)Cyr61和CTGF mRNA在重度子痫前期患者胎盘组织的表达水平呈负相关(P<0.01)。结论:Cyr61表达下调和CTGF过度表达与子痫前期的发病有关,并在该病的发病过程中起到一定的作用。
