目的对儿童过敏性紫癜性肾炎(henoch-schonlein purpura nephritis,HSPN)细胞因子信号转导抑制蛋白3(suppressor of cytokine signaling-3,SOCS-3)及高迁移率族蛋白B1(high mobility group protein B1,HMGB1)与辅助性T细胞/调节性T细胞(...目的对儿童过敏性紫癜性肾炎(henoch-schonlein purpura nephritis,HSPN)细胞因子信号转导抑制蛋白3(suppressor of cytokine signaling-3,SOCS-3)及高迁移率族蛋白B1(high mobility group protein B1,HMGB1)与辅助性T细胞/调节性T细胞(helper T cell 17/regulate T cell,Th17/Treg)细胞失衡的关系进行探讨。方法选取HSPN患儿100例作为研究对象,将其纳入HSPN组,同时选取同期在我院过敏性紫癜患儿100例作为对照组,分别于2组入院时抽取空腹静脉血,比较2组HMGB1、SOCS3 mRNA、Th17、Treg检测结果,并计算Th17/Treg水平,利用ROC分析HMGB1、SOCS3 mRNA、Th17、Treg、Th17/Treg预测患儿发生HSPN的价值,通过person系数分析HMGB1、SOCS3 mRNA与Th17、Treg、Th17/Treg的相关性。结果HSPN组HMGB1、SOCS3 mRNA、Th17、Th17/Treg显著高于对照组,Treg水平显著低于对照组,差异有统计学意义(P<0.05)。经ROC分析,HMGB1、SOCS3 mRNA、Th17、Treg、Th17/Treg预测患儿发生HSPN的曲线下面积分别为0.944、0.924、0.942、0.973、0.955。经相关性分析,HMGB1、SOCS3 mRNA与Th17、Th17/Treg呈明显正相关,与Treg呈明显负相关(P<0.05)。结论小儿HSPN体内SOCS-3及HMGB1表达与Th17/Treg细胞失衡有较高的相关性。展开更多
Objective: To investigate the effect of Astragalus Injection on the Skin Lesion Degree and Caspase-14, SOCS1 and STAT3 Levels of Psoriasis Model in Balb/c Nude Mice. Methods:Sixty Balb/c nude mice were randomly divide...Objective: To investigate the effect of Astragalus Injection on the Skin Lesion Degree and Caspase-14, SOCS1 and STAT3 Levels of Psoriasis Model in Balb/c Nude Mice. Methods:Sixty Balb/c nude mice were randomly divided into groups A, B and C with 20 mice in each group. Group A mice were used as blank control, group B mice as model group and group C mice as treatment group. The PASI score of psoriasis, skin thickness, inflammatory factors, serum levels of Caspase-14, SOCS1 and STAT3 in three groups of mice were analyzed after 2 weeks of treatment. Result: After treatment, the P ASI score of group B was significantly higher than that of group C, with statistical significance (P < 0.05);there was statistical significance in the measurements of lesion skin of three groups of mice after treatment (P <0.05). Compared with the blank control group, the thickness of lesion skin in group B and C was significantly higher, and the thickness of lesion skin in treatment group was significantly lower than that in control group (P < 0.05). Compared with the blank control group, the inflammatory factors IL-17, IL-22 and IL-23 in the B and C groups were significantly increased, and the inflammatory factors IL-17, IL-22 and IL-23 in the treatment group were significantly lower than those in the model group. The levels of serum C aspase-14, SOCS1 and STAT3 in three groups of mice were significantly different after treatment (P < 0.05). Compared with the blank control group, the levels of serum C aspase-14 and SOCS1 in B and C groups were significantly lower and the levels of STAT3 were significantly higher, and the levels of inflammatory factors aspase-14 and SO in treatment group were significantly higher than those in control group. The level of CS1 was significantly lower than that of model group, and the level of STAT3 was significantly higher than that of model group (P <0.05). Conclusion: Astragalus membranaceus injection can effectively improve the degree of psoriasis in Balb/c nude mice. Its possible mechanism is that it can decrease the expression of Caspase-14 and SOCS1, reduce the degree of keratosis in the lesion site of mice, improve the local surface hyperplasia, increase the level of STAT3 and enhance the level of local cell proliferation, which is of positive significance for the rehabilitation of psoriasis.展开更多
文摘目的对儿童过敏性紫癜性肾炎(henoch-schonlein purpura nephritis,HSPN)细胞因子信号转导抑制蛋白3(suppressor of cytokine signaling-3,SOCS-3)及高迁移率族蛋白B1(high mobility group protein B1,HMGB1)与辅助性T细胞/调节性T细胞(helper T cell 17/regulate T cell,Th17/Treg)细胞失衡的关系进行探讨。方法选取HSPN患儿100例作为研究对象,将其纳入HSPN组,同时选取同期在我院过敏性紫癜患儿100例作为对照组,分别于2组入院时抽取空腹静脉血,比较2组HMGB1、SOCS3 mRNA、Th17、Treg检测结果,并计算Th17/Treg水平,利用ROC分析HMGB1、SOCS3 mRNA、Th17、Treg、Th17/Treg预测患儿发生HSPN的价值,通过person系数分析HMGB1、SOCS3 mRNA与Th17、Treg、Th17/Treg的相关性。结果HSPN组HMGB1、SOCS3 mRNA、Th17、Th17/Treg显著高于对照组,Treg水平显著低于对照组,差异有统计学意义(P<0.05)。经ROC分析,HMGB1、SOCS3 mRNA、Th17、Treg、Th17/Treg预测患儿发生HSPN的曲线下面积分别为0.944、0.924、0.942、0.973、0.955。经相关性分析,HMGB1、SOCS3 mRNA与Th17、Th17/Treg呈明显正相关,与Treg呈明显负相关(P<0.05)。结论小儿HSPN体内SOCS-3及HMGB1表达与Th17/Treg细胞失衡有较高的相关性。
文摘Objective: To investigate the effect of Astragalus Injection on the Skin Lesion Degree and Caspase-14, SOCS1 and STAT3 Levels of Psoriasis Model in Balb/c Nude Mice. Methods:Sixty Balb/c nude mice were randomly divided into groups A, B and C with 20 mice in each group. Group A mice were used as blank control, group B mice as model group and group C mice as treatment group. The PASI score of psoriasis, skin thickness, inflammatory factors, serum levels of Caspase-14, SOCS1 and STAT3 in three groups of mice were analyzed after 2 weeks of treatment. Result: After treatment, the P ASI score of group B was significantly higher than that of group C, with statistical significance (P < 0.05);there was statistical significance in the measurements of lesion skin of three groups of mice after treatment (P <0.05). Compared with the blank control group, the thickness of lesion skin in group B and C was significantly higher, and the thickness of lesion skin in treatment group was significantly lower than that in control group (P < 0.05). Compared with the blank control group, the inflammatory factors IL-17, IL-22 and IL-23 in the B and C groups were significantly increased, and the inflammatory factors IL-17, IL-22 and IL-23 in the treatment group were significantly lower than those in the model group. The levels of serum C aspase-14, SOCS1 and STAT3 in three groups of mice were significantly different after treatment (P < 0.05). Compared with the blank control group, the levels of serum C aspase-14 and SOCS1 in B and C groups were significantly lower and the levels of STAT3 were significantly higher, and the levels of inflammatory factors aspase-14 and SO in treatment group were significantly higher than those in control group. The level of CS1 was significantly lower than that of model group, and the level of STAT3 was significantly higher than that of model group (P <0.05). Conclusion: Astragalus membranaceus injection can effectively improve the degree of psoriasis in Balb/c nude mice. Its possible mechanism is that it can decrease the expression of Caspase-14 and SOCS1, reduce the degree of keratosis in the lesion site of mice, improve the local surface hyperplasia, increase the level of STAT3 and enhance the level of local cell proliferation, which is of positive significance for the rehabilitation of psoriasis.
