Cytokinesis-blocked micronucleus assay was applied as a biological dosimeter to detect abnormalities in human peripheral lymphocytes of thirteen students exposed to formaldehyde (FA) during a 12-week (10 h per week) a...Cytokinesis-blocked micronucleus assay was applied as a biological dosimeter to detect abnormalities in human peripheral lymphocytes of thirteen students exposed to formaldehyde (FA) during a 12-week (10 h per week) anatomy class. Breathing-zone air samples colleeted during dissection procedures showed a mean concentration of 2. 37 ppm (3. 17mg/m3 ). Ten students from the same school but without FA exposure served as controls. Chromosome aberrations (CA) and sister chromatid exchanges (SCE) were detected in both groups. The micronuclei (MN) rate (6. 38 ± 2. 50‰ ) and CA rate (5. 92 ±2. 40‰ ) in the FA-exposed group showed a significant increase (P< 0. 01 ) when compared with those of the controls (3. 15 ±1. 46‰and 3. 40 ± 1. 57 % respectively). A correlation between MN and CA in individuals was observed. SCE in the exmpd group were also increased (P< 0. 05), but not so greatly as MN or CA. The results indicated that FA might damage the chromosomes of human lymphocytes.展开更多
Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dos...Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dosimeters to detect DNA damage and abnormalities in human peripheral lymphocytes of subpopulation exposed to X ray radiation The subjects were divided into four groups: 12 radiation patients; 13 intervention radiation therapy doctors; 32 radiation diagnostians; 28 controls Results The average comet lengths of the four groups were 128 17±4 49?μm, 88 09±5 39?μm, 72 68±2 57?μm and 32 87±0 57?μm, respectively The difference in average comet length between any two groups was highly significant ( P <0 01) The average micronucleated cell (MNC) rates (‰) of the four groups were 12 33±0 85, 9 75±1 02, 8 48±0 66 and 3 18±0 36, respectively The difference of MNC rates of Group 1 vs 3, 1 vs 4, 2 vs 4 and 3 vs 4 was highly significant ( P <0 01), and the difference of Group 1 vs 2 was significant ( P <0 05), but there was no difference of MNC rate in Group 2 vs 3 ( P >0 05) Conclusions This study showed that both the comet assay and the CBMN test could be used to monitor populations exposed to X ray radiation, but the comet assay seems to be more sensitive than the CBMN test展开更多
Cytogenetic indicators,especially dicentric chromosomes,have been globally considered the gold standard of biological dose estimation for more than 60 years.The application of these indicators to biological dose estim...Cytogenetic indicators,especially dicentric chromosomes,have been globally considered the gold standard of biological dose estimation for more than 60 years.The application of these indicators to biological dose estimation in victims of radiation accidents in China has undergone approximately 40 years of development and thus provides more accurate information for the clinical diagnosis and patients’treatment.This paper reviews the application and future development of cytogenetic indicators,including chromosomal aberrations(dicentric chromosomes and chromosomal translocations),cytokinesis-block micronuclei,nucleoplasmic bridges,and premature condensation chromosome ring,as biodosimeters in China.展开更多
文摘Cytokinesis-blocked micronucleus assay was applied as a biological dosimeter to detect abnormalities in human peripheral lymphocytes of thirteen students exposed to formaldehyde (FA) during a 12-week (10 h per week) anatomy class. Breathing-zone air samples colleeted during dissection procedures showed a mean concentration of 2. 37 ppm (3. 17mg/m3 ). Ten students from the same school but without FA exposure served as controls. Chromosome aberrations (CA) and sister chromatid exchanges (SCE) were detected in both groups. The micronuclei (MN) rate (6. 38 ± 2. 50‰ ) and CA rate (5. 92 ±2. 40‰ ) in the FA-exposed group showed a significant increase (P< 0. 01 ) when compared with those of the controls (3. 15 ±1. 46‰and 3. 40 ± 1. 57 % respectively). A correlation between MN and CA in individuals was observed. SCE in the exmpd group were also increased (P< 0. 05), but not so greatly as MN or CA. The results indicated that FA might damage the chromosomes of human lymphocytes.
基金ThisresearchwassupportedbytheNaturalScienceFoundationofZhejiangProvince China (No 396 490 )
文摘Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dosimeters to detect DNA damage and abnormalities in human peripheral lymphocytes of subpopulation exposed to X ray radiation The subjects were divided into four groups: 12 radiation patients; 13 intervention radiation therapy doctors; 32 radiation diagnostians; 28 controls Results The average comet lengths of the four groups were 128 17±4 49?μm, 88 09±5 39?μm, 72 68±2 57?μm and 32 87±0 57?μm, respectively The difference in average comet length between any two groups was highly significant ( P <0 01) The average micronucleated cell (MNC) rates (‰) of the four groups were 12 33±0 85, 9 75±1 02, 8 48±0 66 and 3 18±0 36, respectively The difference of MNC rates of Group 1 vs 3, 1 vs 4, 2 vs 4 and 3 vs 4 was highly significant ( P <0 01), and the difference of Group 1 vs 2 was significant ( P <0 05), but there was no difference of MNC rate in Group 2 vs 3 ( P >0 05) Conclusions This study showed that both the comet assay and the CBMN test could be used to monitor populations exposed to X ray radiation, but the comet assay seems to be more sensitive than the CBMN test
基金This work was supported by Key Science and Technology Project of Henan Province,China,IDs:142102310086Medical Science and Technology Reseach of Henan Province,China,IDs:LHGJ20190815。
文摘Cytogenetic indicators,especially dicentric chromosomes,have been globally considered the gold standard of biological dose estimation for more than 60 years.The application of these indicators to biological dose estimation in victims of radiation accidents in China has undergone approximately 40 years of development and thus provides more accurate information for the clinical diagnosis and patients’treatment.This paper reviews the application and future development of cytogenetic indicators,including chromosomal aberrations(dicentric chromosomes and chromosomal translocations),cytokinesis-block micronuclei,nucleoplasmic bridges,and premature condensation chromosome ring,as biodosimeters in China.
