Background Fatty liver disease causes huge economic losses in the poultry industry due to its high occurrence and lethality rate.Three-dimensional(3D)chromatin architecture takes part in disease processing by regulati...Background Fatty liver disease causes huge economic losses in the poultry industry due to its high occurrence and lethality rate.Three-dimensional(3D)chromatin architecture takes part in disease processing by regulating tran-scriptional reprogramming.The study is carried out to investigate the alterations of hepatic 3D genome and H3K27ac profiling in early fatty liver(FLS)and reveal their effect on hepatic transcriptional reprogramming in laying hens.Results Results show that FLS model is constructed with obvious phenotypes including hepatic visible lipid deposi-tion as well as higher total triglyceride and cholesterol in serum.A/B compartment switching,topologically associat-ing domain(TAD)and chromatin loop changes are identified by high-throughput/resolution chromosome conforma-tion capture(HiC)technology.Targeted genes of these alternations in hepatic 3D genome organization significantly enrich pathways related to lipid metabolism and hepatic damage.H3K27ac differential peaks and differential expres-sion genes(DEGs)identified through RNA-seq analysis are also enriched in these pathways.Notably,certain DEGs are found to correspond with changes in 3D chromatin structure and H3K27ac binding in their promoters.DNA motif analysis reveals that candidate transcription factors are implicated in regulating transcriptional reprogram-ming.Furthermore,disturbed folate metabolism is observed,as evidenced by lower folate levels and altered enzyme expression.Conclusion Our findings establish a link between transcriptional reprogramming changes and 3D chromatin struc-ture variations during early FLS formation,which provides candidate transcription factors and folate as targets for FLS prevention or treatment.展开更多
Based on ecocriticism theory,this paper is to conduct a comprehensive discussion of Lawrence’s poetry from three aspects:natural ecology,social ecology and spiritual ecology,to explore the profound ecological philoso...Based on ecocriticism theory,this paper is to conduct a comprehensive discussion of Lawrence’s poetry from three aspects:natural ecology,social ecology and spiritual ecology,to explore the profound ecological philosophical implications contained in Lawrence’s poetry,and to expand the breadth and depth of the ecocriticism interpretation of Lowe’s poetry research.展开更多
This thesis focuses on D.H.Lawrence's view on female in his novel writing. The author firstly introduces Lawrence's view on female through analyzing female images in his works,then analyzes the contributing fa...This thesis focuses on D.H.Lawrence's view on female in his novel writing. The author firstly introduces Lawrence's view on female through analyzing female images in his works,then analyzes the contributing factors of his views' formation.展开更多
Through ecocritical study of Odour of Chrysanthemums,this paper reflects that the industrial civilization leads to damaged nature,distorted humanity and conjugal relation.
As probably the most misread writer of the 20th century, D. H. Lawrence was not only a great novelist, but also a great poet, who should have shared the same importance as W. B. Yeats and T. S. Eliot."Bavarian Ge...As probably the most misread writer of the 20th century, D. H. Lawrence was not only a great novelist, but also a great poet, who should have shared the same importance as W. B. Yeats and T. S. Eliot."Bavarian Gentians"is one of his masterpieces, which was written in the last few months of his life along with some other poems on death, heaven and hell, including The Ship of Death, Glory of Darkness and Song of Death. The mythological poem, a bit mystic and surreal, embodies the view of Lawrence on death. This paper is trying to give a brief analysis on the images and theme of the poem.展开更多
CX]\] D. H. Lawrence insists that a kind of manly love -- blood brotherhood be one of the indispensable factors for men’s future consummation of life, nevertheless, he doesn’tsatisfactorily solve the problem in this...CX]\] D. H. Lawrence insists that a kind of manly love -- blood brotherhood be one of the indispensable factors for men’s future consummation of life, nevertheless, he doesn’tsatisfactorily solve the problem in this book.[展开更多
David Herbert Lawrence is a modernist who remains controversial in literary criticism even now. The major point of debate about him is his perception of women and the relation between the two sexes. Despite his misogy...David Herbert Lawrence is a modernist who remains controversial in literary criticism even now. The major point of debate about him is his perception of women and the relation between the two sexes. Despite his misogynic pronouncements in his essays and feminist critics' constant attack on him, this article argues that Lawrence does not hold a male chauvinist or antifemale perspective in his fiction. Through a careful survey of his major novels and short stories, this article explores the rich variety of female characters that he created and his deep insight into women's existence, and identifies him as a male writer struggling to speak for women from a non-feminist standpoint.展开更多
David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes.Despi...David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes.Despite his misogynic pronouncements in his essays and feminist critics’constant attack on him,this paper,through a careful survey of his major novels and short stories,explores the rich variety of aggressive female characters that he created and his deep insight into women’s existence,and identifies him as a male writer struggling to speak for women from a non-feminist standpoint.展开更多
David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes. This...David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes. This article,through a careful survey of his major novels and short stories, explores a wide range of oppressive female characters that he created and his deep insight into women's existence. Rather than simply demonize women in his fiction, D.H. Lawrence intends to reveal to the reader that women's will to serve, to sacrifice, and to be adored may well evolve into a strong desire to manipulate and monopolize their men. In response the author repeatedly advocates and eulogizes a relationship of spiritual separateness in balance with physical union between the sexes.展开更多
Lady Chatterley’s Lover is David Herbert Lawrence’s last novel of elaborating sexual relationship in extramarital affairs.Since the novel was published in 1928,scholars have been studying it from various perspective...Lady Chatterley’s Lover is David Herbert Lawrence’s last novel of elaborating sexual relationship in extramarital affairs.Since the novel was published in 1928,scholars have been studying it from various perspectives.Lawrence depicts intercourse between the two protagonists explicitly by actions,discourses and mental acts,and he once justified the publication of this book,which provides possibility and clues for probing his view of sex and sexual relationship through the novel.Staring from Mellors’discourses with Connie,the description of male characters,and the anima and animus archetypes in the novel,the paper analyzes Lawrence’s sexual theory and his advocacy for combination of body and soul based on the British society then and Lawrence’s own experience.展开更多
In Women in Love (1921) the reader can easily perceive the author’s abhorrence to European industrial civilization. To D. H. Lawrence,the inescapable fate of this sort of social deveolpment is destruction. And he tak...In Women in Love (1921) the reader can easily perceive the author’s abhorrence to European industrial civilization. To D. H. Lawrence,the inescapable fate of this sort of social deveolpment is destruction. And he takes it for granted that"Rananim"——the Lawrencian utopia, is a social outlet for human salvation.展开更多
目的研究24h血乳酸(Lac)清除率联合外周血中性粒细胞与淋巴细胞比值(NLR)、降钙素原(PCT)对重症监护病房(ICU)重症肺炎患者28 d病死风险的预测价值。方法回顾性选取2017年12月—2021年12月安徽皖北煤电集团总医院78例重症肺炎患者作为...目的研究24h血乳酸(Lac)清除率联合外周血中性粒细胞与淋巴细胞比值(NLR)、降钙素原(PCT)对重症监护病房(ICU)重症肺炎患者28 d病死风险的预测价值。方法回顾性选取2017年12月—2021年12月安徽皖北煤电集团总医院78例重症肺炎患者作为研究对象,根据28d临床结局将其分为病死组27例和存活组51例。比较两组的一般资料、24hLac清除率、NLR、PCT,采用多因素一般Logistic回归模型分析28d内病死的影响因素,采用受试者工作特征(ROC)曲线评估24hLac清除率联合NLR、PCT对28d内病死风险的预测价值。结果两组的性别、年龄、吸烟史、基础疾病、氧合指数、体温、平均动脉压、白细胞计数、血小板计数、抗菌药物使用时间比较,差异均无统计学意义(P>0.05);病死组肺炎严重程度评分(PSI)、急性生理学和慢性健康状况评价Ⅱ(APACHEⅡ)评分、机械通气治疗占比均高于存活组(P<0.05);病死组24 h Lac清除率低于存活组,NLR、PCT水平高于存活组(P<0.05);经多因素一般Logistic回归分析,PSI评分[O^R=9.440(95%CI:4.108,21.697)]、APACHEⅡ评分[O^R=3.904(95%CI:1.699,8.972)]、24 h Lac清除率[O^R=5.068(95%CI:2.205,11.648)]、NLR[O^R=8.199(95%CI:3.567,18.843)]、PCT[O^R=5.766(95%CI:2.509,13.252)]均为28 d内病死的影响因素(P<0.05);ROC曲线分析结果显示,24 h Lac清除率、NLR、PCT预测28 d内病死的最佳截断值为26.10%、5.95和11.27μg/L,敏感性为81.48%(95%CI:0.613,0.930)、59.26%(95%CI:0.390,0.770)、66.67%(95%CI:0.460,0.828),特异性为72.55%(95%CI:0.580,0.837)、82.35%(95%CI:0.686,0.911)、90.20%(95%CI:0.778,0.963),AUC值为0.809(95%CI:0.704,0.889)、0.736(95%CI:0.624,0.830)、0.802(95%CI:0.697,0.884),联合预测的敏感性为92.59%(95%CI:0.742,0.987)、特异性为86.27%(95%CI:0.731,0.938)、AUC值为0.946(95%CI:0.871,0.985)。结论ICU重症肺炎28 d内病死患者的24 h Lac清除率、NLR、PCT明显异常变化,其对预后评估具有良好预测价值。展开更多
基金funded by the National Science Foundation of China (32372910 and 32102567)the Program for Shaanxi Science&Technology (2022KJXX-13, 2023-YBNY-144, K3031223077 and 2022GD-TSLD-46–0302)
文摘Background Fatty liver disease causes huge economic losses in the poultry industry due to its high occurrence and lethality rate.Three-dimensional(3D)chromatin architecture takes part in disease processing by regulating tran-scriptional reprogramming.The study is carried out to investigate the alterations of hepatic 3D genome and H3K27ac profiling in early fatty liver(FLS)and reveal their effect on hepatic transcriptional reprogramming in laying hens.Results Results show that FLS model is constructed with obvious phenotypes including hepatic visible lipid deposi-tion as well as higher total triglyceride and cholesterol in serum.A/B compartment switching,topologically associat-ing domain(TAD)and chromatin loop changes are identified by high-throughput/resolution chromosome conforma-tion capture(HiC)technology.Targeted genes of these alternations in hepatic 3D genome organization significantly enrich pathways related to lipid metabolism and hepatic damage.H3K27ac differential peaks and differential expres-sion genes(DEGs)identified through RNA-seq analysis are also enriched in these pathways.Notably,certain DEGs are found to correspond with changes in 3D chromatin structure and H3K27ac binding in their promoters.DNA motif analysis reveals that candidate transcription factors are implicated in regulating transcriptional reprogram-ming.Furthermore,disturbed folate metabolism is observed,as evidenced by lower folate levels and altered enzyme expression.Conclusion Our findings establish a link between transcriptional reprogramming changes and 3D chromatin struc-ture variations during early FLS formation,which provides candidate transcription factors and folate as targets for FLS prevention or treatment.
基金Acknowledgement:this paper is funded by Project:The final research result of“World Language and Culture Research”project of China Center for Language Planning and Policy Studies。
文摘Based on ecocriticism theory,this paper is to conduct a comprehensive discussion of Lawrence’s poetry from three aspects:natural ecology,social ecology and spiritual ecology,to explore the profound ecological philosophical implications contained in Lawrence’s poetry,and to expand the breadth and depth of the ecocriticism interpretation of Lowe’s poetry research.
文摘This thesis focuses on D.H.Lawrence's view on female in his novel writing. The author firstly introduces Lawrence's view on female through analyzing female images in his works,then analyzes the contributing factors of his views' formation.
文摘Through ecocritical study of Odour of Chrysanthemums,this paper reflects that the industrial civilization leads to damaged nature,distorted humanity and conjugal relation.
文摘As probably the most misread writer of the 20th century, D. H. Lawrence was not only a great novelist, but also a great poet, who should have shared the same importance as W. B. Yeats and T. S. Eliot."Bavarian Gentians"is one of his masterpieces, which was written in the last few months of his life along with some other poems on death, heaven and hell, including The Ship of Death, Glory of Darkness and Song of Death. The mythological poem, a bit mystic and surreal, embodies the view of Lawrence on death. This paper is trying to give a brief analysis on the images and theme of the poem.
文摘CX]\] D. H. Lawrence insists that a kind of manly love -- blood brotherhood be one of the indispensable factors for men’s future consummation of life, nevertheless, he doesn’tsatisfactorily solve the problem in this book.[
文摘David Herbert Lawrence is a modernist who remains controversial in literary criticism even now. The major point of debate about him is his perception of women and the relation between the two sexes. Despite his misogynic pronouncements in his essays and feminist critics' constant attack on him, this article argues that Lawrence does not hold a male chauvinist or antifemale perspective in his fiction. Through a careful survey of his major novels and short stories, this article explores the rich variety of female characters that he created and his deep insight into women's existence, and identifies him as a male writer struggling to speak for women from a non-feminist standpoint.
文摘David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes.Despite his misogynic pronouncements in his essays and feminist critics’constant attack on him,this paper,through a careful survey of his major novels and short stories,explores the rich variety of aggressive female characters that he created and his deep insight into women’s existence,and identifies him as a male writer struggling to speak for women from a non-feminist standpoint.
文摘David Herbert Lawrence is one of the few male modernists who remain controversial in literary criticism even now.The major point of debate about him is his outlook on women and the relation between the two sexes. This article,through a careful survey of his major novels and short stories, explores a wide range of oppressive female characters that he created and his deep insight into women's existence. Rather than simply demonize women in his fiction, D.H. Lawrence intends to reveal to the reader that women's will to serve, to sacrifice, and to be adored may well evolve into a strong desire to manipulate and monopolize their men. In response the author repeatedly advocates and eulogizes a relationship of spiritual separateness in balance with physical union between the sexes.
文摘Lady Chatterley’s Lover is David Herbert Lawrence’s last novel of elaborating sexual relationship in extramarital affairs.Since the novel was published in 1928,scholars have been studying it from various perspectives.Lawrence depicts intercourse between the two protagonists explicitly by actions,discourses and mental acts,and he once justified the publication of this book,which provides possibility and clues for probing his view of sex and sexual relationship through the novel.Staring from Mellors’discourses with Connie,the description of male characters,and the anima and animus archetypes in the novel,the paper analyzes Lawrence’s sexual theory and his advocacy for combination of body and soul based on the British society then and Lawrence’s own experience.
文摘In Women in Love (1921) the reader can easily perceive the author’s abhorrence to European industrial civilization. To D. H. Lawrence,the inescapable fate of this sort of social deveolpment is destruction. And he takes it for granted that"Rananim"——the Lawrencian utopia, is a social outlet for human salvation.
文摘目的研究24h血乳酸(Lac)清除率联合外周血中性粒细胞与淋巴细胞比值(NLR)、降钙素原(PCT)对重症监护病房(ICU)重症肺炎患者28 d病死风险的预测价值。方法回顾性选取2017年12月—2021年12月安徽皖北煤电集团总医院78例重症肺炎患者作为研究对象,根据28d临床结局将其分为病死组27例和存活组51例。比较两组的一般资料、24hLac清除率、NLR、PCT,采用多因素一般Logistic回归模型分析28d内病死的影响因素,采用受试者工作特征(ROC)曲线评估24hLac清除率联合NLR、PCT对28d内病死风险的预测价值。结果两组的性别、年龄、吸烟史、基础疾病、氧合指数、体温、平均动脉压、白细胞计数、血小板计数、抗菌药物使用时间比较,差异均无统计学意义(P>0.05);病死组肺炎严重程度评分(PSI)、急性生理学和慢性健康状况评价Ⅱ(APACHEⅡ)评分、机械通气治疗占比均高于存活组(P<0.05);病死组24 h Lac清除率低于存活组,NLR、PCT水平高于存活组(P<0.05);经多因素一般Logistic回归分析,PSI评分[O^R=9.440(95%CI:4.108,21.697)]、APACHEⅡ评分[O^R=3.904(95%CI:1.699,8.972)]、24 h Lac清除率[O^R=5.068(95%CI:2.205,11.648)]、NLR[O^R=8.199(95%CI:3.567,18.843)]、PCT[O^R=5.766(95%CI:2.509,13.252)]均为28 d内病死的影响因素(P<0.05);ROC曲线分析结果显示,24 h Lac清除率、NLR、PCT预测28 d内病死的最佳截断值为26.10%、5.95和11.27μg/L,敏感性为81.48%(95%CI:0.613,0.930)、59.26%(95%CI:0.390,0.770)、66.67%(95%CI:0.460,0.828),特异性为72.55%(95%CI:0.580,0.837)、82.35%(95%CI:0.686,0.911)、90.20%(95%CI:0.778,0.963),AUC值为0.809(95%CI:0.704,0.889)、0.736(95%CI:0.624,0.830)、0.802(95%CI:0.697,0.884),联合预测的敏感性为92.59%(95%CI:0.742,0.987)、特异性为86.27%(95%CI:0.731,0.938)、AUC值为0.946(95%CI:0.871,0.985)。结论ICU重症肺炎28 d内病死患者的24 h Lac清除率、NLR、PCT明显异常变化,其对预后评估具有良好预测价值。
