目的:通过观察β-半乳糖苷结合蛋白-3(Galectin-3)与β-葡萄糖醛酸苷酶(β-G)在大肠癌组织中的表达,与大肠癌的生物学特征进行比较,以探讨其临床意义.方法:采用SP免疫组织化学方法检测98例临床新鲜标本,其中包括15例癌旁正常大肠黏膜组...目的:通过观察β-半乳糖苷结合蛋白-3(Galectin-3)与β-葡萄糖醛酸苷酶(β-G)在大肠癌组织中的表达,与大肠癌的生物学特征进行比较,以探讨其临床意义.方法:采用SP免疫组织化学方法检测98例临床新鲜标本,其中包括15例癌旁正常大肠黏膜组织及83例大肠癌组织中galectin-3和β-G的表达情况,并进行两种蛋白的表达相关性分析.结果:Galectin-3和β-G蛋白在浸润程度深、分化程度低及Dukes分期较晚的大肠癌组织中的表达高于浸润程度浅(0.15±0.03 vs 0.16±0.05,P=0.02)、分化程度高(0.15±0.012 vs 0.16±0.03,P=0.03)及Dukes分期较早(0.18±0.06 vs 0.17±0.05)的大肠癌组织(P<0.05);在大肠癌组织中,ga-lectin-3的表达和β-G的表达呈正相关(r=0.628,P<0.01).结论:Galectin-3和β-G的异常表达在大肠癌的发生、发展中起着重要作用,具有一定的诊断价值.展开更多
stick moxibustion on the "Baihui" and "Dazhui" of mice of aging model induced by D-galactose,and then hippocampus of mice in normal control group, modeling group and stick moxibustion groupwas gott...stick moxibustion on the "Baihui" and "Dazhui" of mice of aging model induced by D-galactose,and then hippocampus of mice in normal control group, modeling group and stick moxibustion groupwas gotton respectively, synaptosomal protein was extracted and analyzed by SDS -PAGE and Minorlayer scanning. The results showed that, in modeling group of D-galactose, changes of relative contentof eight types of protein in hippocampi were greater than 5 %, among them, 4 was up and 4 was down.Above 8 types of protein in stick moxibustion curing group, except for 36 KDa protein, other 7 types ofrelative content proteins changed compared with modeling group. Protein increased (or decreased) inmodeling group decreased (or increased) in curing group. It maybe suggest that stick moxibustion hasgood dual regulation to the disadvantageous effect of D- galactose on hippocampal synaptosomal proteins of展开更多
文摘目的:通过观察β-半乳糖苷结合蛋白-3(Galectin-3)与β-葡萄糖醛酸苷酶(β-G)在大肠癌组织中的表达,与大肠癌的生物学特征进行比较,以探讨其临床意义.方法:采用SP免疫组织化学方法检测98例临床新鲜标本,其中包括15例癌旁正常大肠黏膜组织及83例大肠癌组织中galectin-3和β-G的表达情况,并进行两种蛋白的表达相关性分析.结果:Galectin-3和β-G蛋白在浸润程度深、分化程度低及Dukes分期较晚的大肠癌组织中的表达高于浸润程度浅(0.15±0.03 vs 0.16±0.05,P=0.02)、分化程度高(0.15±0.012 vs 0.16±0.03,P=0.03)及Dukes分期较早(0.18±0.06 vs 0.17±0.05)的大肠癌组织(P<0.05);在大肠癌组织中,ga-lectin-3的表达和β-G的表达呈正相关(r=0.628,P<0.01).结论:Galectin-3和β-G的异常表达在大肠癌的发生、发展中起着重要作用,具有一定的诊断价值.
文摘stick moxibustion on the "Baihui" and "Dazhui" of mice of aging model induced by D-galactose,and then hippocampus of mice in normal control group, modeling group and stick moxibustion groupwas gotton respectively, synaptosomal protein was extracted and analyzed by SDS -PAGE and Minorlayer scanning. The results showed that, in modeling group of D-galactose, changes of relative contentof eight types of protein in hippocampi were greater than 5 %, among them, 4 was up and 4 was down.Above 8 types of protein in stick moxibustion curing group, except for 36 KDa protein, other 7 types ofrelative content proteins changed compared with modeling group. Protein increased (or decreased) inmodeling group decreased (or increased) in curing group. It maybe suggest that stick moxibustion hasgood dual regulation to the disadvantageous effect of D- galactose on hippocampal synaptosomal proteins of
