[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny a...[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.展开更多
D-T_(2)two-dimensional nuclear magnetic resonance(2D NMR)logging technology can distinguish pore fluid types intuitively,and it is widely used in oil and gas exploration.Many 2D NMR inversion methods(e.g.,truncated si...D-T_(2)two-dimensional nuclear magnetic resonance(2D NMR)logging technology can distinguish pore fluid types intuitively,and it is widely used in oil and gas exploration.Many 2D NMR inversion methods(e.g.,truncated singular value decomposition(TSVD),Butler-Reds-Dawson(BRD),LM-norm smoothing,and TIST-L1 regularization methods)have been proposed successively,but most are limited to numerical simulations.This study focused on the applicability of different inversion methods for NMR logging data of various acquisition sequences,from which the optimal inversion method was selected based on the comparative analysis.First,the two-dimensional NMR logging principle was studied.Then,these inversion methods were studied in detail,and the precision and computational efficiency of CPMG and diffusion editing(DE)sequences obtained from oil-water and gas-water models were compared,respectively.The inversion results and calculation time of truncated singular value decomposition(TSVD),Butler-Reds-Dawson(BRD),LM-norm smoothing,and TIST-L1 regularization were compared and analyzed through numerical simulations.The inversion method was optimized to process SP mode logging data from the MR Scanner instrument.The results showed that the TIST-regularization and LM-norm smoothing methods were more accurate for the CPMG and DE sequence echo trains of the oil-water and gas-water models.However,the LM-norm smoothing method was less time-consuming,making it more suitable for logging data processing.A case study in well A25 showed that the processing results by the LM-norm smoothing method were consistent with GEOLOG software.This demonstrates that the LM-norm smoothing method is applicable in practical NMR logging processing.展开更多
To make sure that the process of jacket launch occurs in a seml-controlled manner, this paper deals with measurement of kinematic parameters of jacket launch using stereo vision and motion analysis. The system capture...To make sure that the process of jacket launch occurs in a seml-controlled manner, this paper deals with measurement of kinematic parameters of jacket launch using stereo vision and motion analysis. The system captured stereo image sequences by two separate CCD cameras, and then rebuilt 3D coordinates of the feature points to analyze the jacket launch motion. The possibility of combining stereo vision and motion analysis for measurement was examined. Resuhs by experiments using scale model of jacket confirm the theoretical data.展开更多
Cathepsin D （CTSD） is a lysosomal acidic endoproteinase that plays an important role in immune response, In this study, we obtained sweetfish （Plecoglossus altivelis） CTSD （PaCTSD） via de-novo transcriptome sequ...Cathepsin D （CTSD） is a lysosomal acidic endoproteinase that plays an important role in immune response, In this study, we obtained sweetfish （Plecoglossus altivelis） CTSD （PaCTSD） via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17x 103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish.展开更多
Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China. We used mi- tochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven...Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China. We used mi- tochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven Himalayan snowcock T. himalayensis. The fragments of 1155 nucleotides from the control region of mitochondrial DNA were sequenced, and 57 poly- morphic positions defined 37 haplotypes. A high level of genetic diversity was detected in all populations sampled and may be associated isolation of the mountains and habitat fragmentation and deterioration from Quaternary glaciations. In the phylogenetic tree, all haplotypes grouped into four groups： clade A （Kunlun Mountains clade）, clade B （Northern Qinghai-Tibetan Plateau clade）, clade C （Tianshan Mountains clade） and clade D （Kalakunlun Mountains clade）. We found a low level of gene flow and significant genetic differentiation among all populations. Based on divergence time we suggest that the divergence of Himalayan snowcock occurred in the middle Pleistocene inter-glaciation, and expansion occurred in the glaciation. Analysis of mtDNA D-loop sequences confirmed demographic population expansion, as did our non-significant mismatch distribution analysis. In conclusion, limited gene flow and a pattern of partial isolation phylogeographic was found in geographic populations of T. hima- layansis based on the analysis on mtDNA D-loop sequences [Current Zoology 57 （6）： 758-767, 2011].展开更多
基金Supported by the Fond for Open Projects of Xinjiang Key Laboratory of Herbivore Nutrition for Meat&Milk Production~~
文摘[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.
基金sponsored by the National Natural Science Foundation of China(Nos.42174149,41774144)the National Major Projects(No.2016ZX05014-001).
文摘D-T_(2)two-dimensional nuclear magnetic resonance(2D NMR)logging technology can distinguish pore fluid types intuitively,and it is widely used in oil and gas exploration.Many 2D NMR inversion methods(e.g.,truncated singular value decomposition(TSVD),Butler-Reds-Dawson(BRD),LM-norm smoothing,and TIST-L1 regularization methods)have been proposed successively,but most are limited to numerical simulations.This study focused on the applicability of different inversion methods for NMR logging data of various acquisition sequences,from which the optimal inversion method was selected based on the comparative analysis.First,the two-dimensional NMR logging principle was studied.Then,these inversion methods were studied in detail,and the precision and computational efficiency of CPMG and diffusion editing(DE)sequences obtained from oil-water and gas-water models were compared,respectively.The inversion results and calculation time of truncated singular value decomposition(TSVD),Butler-Reds-Dawson(BRD),LM-norm smoothing,and TIST-L1 regularization were compared and analyzed through numerical simulations.The inversion method was optimized to process SP mode logging data from the MR Scanner instrument.The results showed that the TIST-regularization and LM-norm smoothing methods were more accurate for the CPMG and DE sequence echo trains of the oil-water and gas-water models.However,the LM-norm smoothing method was less time-consuming,making it more suitable for logging data processing.A case study in well A25 showed that the processing results by the LM-norm smoothing method were consistent with GEOLOG software.This demonstrates that the LM-norm smoothing method is applicable in practical NMR logging processing.
文摘To make sure that the process of jacket launch occurs in a seml-controlled manner, this paper deals with measurement of kinematic parameters of jacket launch using stereo vision and motion analysis. The system captured stereo image sequences by two separate CCD cameras, and then rebuilt 3D coordinates of the feature points to analyze the jacket launch motion. The possibility of combining stereo vision and motion analysis for measurement was examined. Resuhs by experiments using scale model of jacket confirm the theoretical data.
基金Foundation items： The project was supported by the Program for the National Natural Science Foundation of China （31201970, 31372555）, Zhejiang Provincial Natural Science Foundation of China （LZ13C- 190001, LQ13C190002）
文摘Cathepsin D （CTSD） is a lysosomal acidic endoproteinase that plays an important role in immune response, In this study, we obtained sweetfish （Plecoglossus altivelis） CTSD （PaCTSD） via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17x 103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish.
基金Acknowledgments Financial support was provided by the National Natural Science Foundation of China （No. 30530130）, Dr Start-up Fund Research of Qiongzhou Univer- sity （No. QYXB201009） and the Co-operation Fund Between University and Locality, Sanya （No. 2010YD22） for funding this research.
文摘Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China. We used mi- tochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven Himalayan snowcock T. himalayensis. The fragments of 1155 nucleotides from the control region of mitochondrial DNA were sequenced, and 57 poly- morphic positions defined 37 haplotypes. A high level of genetic diversity was detected in all populations sampled and may be associated isolation of the mountains and habitat fragmentation and deterioration from Quaternary glaciations. In the phylogenetic tree, all haplotypes grouped into four groups： clade A （Kunlun Mountains clade）, clade B （Northern Qinghai-Tibetan Plateau clade）, clade C （Tianshan Mountains clade） and clade D （Kalakunlun Mountains clade）. We found a low level of gene flow and significant genetic differentiation among all populations. Based on divergence time we suggest that the divergence of Himalayan snowcock occurred in the middle Pleistocene inter-glaciation, and expansion occurred in the glaciation. Analysis of mtDNA D-loop sequences confirmed demographic population expansion, as did our non-significant mismatch distribution analysis. In conclusion, limited gene flow and a pattern of partial isolation phylogeographic was found in geographic populations of T. hima- layansis based on the analysis on mtDNA D-loop sequences [Current Zoology 57 （6）： 758-767, 2011].
