In this paper,we report the chemical synthesis of oligonucleotide d-TGGGT using phosphotriester method.The protected pentamer d-MmtTGibGibGibT(=p-ClC6H_4-O(O)P)was deblocked by treatment with concentrated ammonium hyd...In this paper,we report the chemical synthesis of oligonucleotide d-TGGGT using phosphotriester method.The protected pentamer d-MmtTGibGibGibT(=p-ClC6H_4-O(O)P)was deblocked by treatment with concentrated ammonium hydroxide and 80% acetic acid.The pure d-TGGGT obtained by chromatorgraphy on DEAE-Sephadex A-25 and Q-Sepharose FF could be hydrolyzed completely and confirmed by base ratio.展开更多
In order to study the action mechanism of cisplatin on DNA,we have used the solution-phase phosphotriester method to synthesize the oligonucleotide d-TGGGT. Prior to investigating the conformational change due to Pt(...In order to study the action mechanism of cisplatin on DNA,we have used the solution-phase phosphotriester method to synthesize the oligonucleotide d-TGGGT. Prior to investigating the conformational change due to Pt(Ⅱ) attachment。展开更多
文摘In this paper,we report the chemical synthesis of oligonucleotide d-TGGGT using phosphotriester method.The protected pentamer d-MmtTGibGibGibT(=p-ClC6H_4-O(O)P)was deblocked by treatment with concentrated ammonium hydroxide and 80% acetic acid.The pure d-TGGGT obtained by chromatorgraphy on DEAE-Sephadex A-25 and Q-Sepharose FF could be hydrolyzed completely and confirmed by base ratio.
文摘In order to study the action mechanism of cisplatin on DNA,we have used the solution-phase phosphotriester method to synthesize the oligonucleotide d-TGGGT. Prior to investigating the conformational change due to Pt(Ⅱ) attachment。
