Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots.Its rapid growth and use as high-quality material make this bamboo species highly valued for both food proces...Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots.Its rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood applications.However,genome information for D.brandisii is lacking,primarily due to its polyploidy and large genome size.Here,we assembled a high-quality genome for hexaploid D.brandisii,which comprises 70chromosomes with a total size of 2,756 Mb,using long-read Hi Fi sequencing.Furthermore,we accurately separated the genome into its three constituent subgenomes.We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly,revealing differential gene expression and post-transcriptional regulation.By integrating metabolome analysis,we unveiled that well-balanced lignin formation,as well as abundant flavonoid and fructose contents,contribute to the superior quality of D.brandisii shoots.Integrating genomic,transcriptomic,and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing techniques.This study should facilitate research on D.brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.展开更多
基金supported by the National Key Research and Development Program of China(2021YFD2200505,2018YFD0600101)the National Natural Science Foundation of China(32071849)。
文摘Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots.Its rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood applications.However,genome information for D.brandisii is lacking,primarily due to its polyploidy and large genome size.Here,we assembled a high-quality genome for hexaploid D.brandisii,which comprises 70chromosomes with a total size of 2,756 Mb,using long-read Hi Fi sequencing.Furthermore,we accurately separated the genome into its three constituent subgenomes.We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly,revealing differential gene expression and post-transcriptional regulation.By integrating metabolome analysis,we unveiled that well-balanced lignin formation,as well as abundant flavonoid and fructose contents,contribute to the superior quality of D.brandisii shoots.Integrating genomic,transcriptomic,and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing techniques.This study should facilitate research on D.brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.
