Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Px...Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg hatching.Subsequent CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be achieved.However,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive capacity.We further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/Cas9.The results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous sequences.We ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in P.xylostella,which can also be used for early sex detection.By injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 gene.Taken together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in P.xylostella,while the establishment of a heritable strain required the positioning of appropriate sites.This study provides an important working basis and technical support for further developing genetic strategies for insect pest control.展开更多
为了实现柔性作业车间完工时间、机器负荷、交货延期时间、车间能耗等多个目标的联合优化,提出了基于自适应惩罚MOEA/D(multi-objective evolutionary algorithm based on decomposition)的柔性车间多目标调度方法。对具有多个生产机床...为了实现柔性作业车间完工时间、机器负荷、交货延期时间、车间能耗等多个目标的联合优化,提出了基于自适应惩罚MOEA/D(multi-objective evolutionary algorithm based on decomposition)的柔性车间多目标调度方法。对具有多个生产机床、多个加工任务、多道工序的柔性车间调度问题进行了描述并建立了优化模型。给出了基于MOEA/D算法的柔性车间调度方法,针对常值惩罚因子无法满足不同邻域对收敛性和染色体多样性不同调整需求的问题,提出了能够随邻域染色体密度自适应调整的惩罚因子,并制定了基于自适应惩罚MOEA/D算法的柔性车间调度流程。在具有8个机床、8个工件共28道工序的生产调度实验中,自适应MOEA/D算法搜索的Pareto前沿解能够支配标准MOEA/D和改进NSGA-Ⅱ算法的Pareto前沿解;在等权重最优解的生产实验中,自适应MOEA/D算法调度方案的完工时间、机器负荷、交货延期时间、车间能耗均小于标准MOEA/D算法和改进NSGA-Ⅱ算法。实验结果证明了自适应惩罚MOEA/D算法在柔性车间调度中的有效性和优越性。展开更多
水提法从蜜环菌 Am 99- 1菌株人工发酵菌体分离得到蜜环菌多糖 Am 1,以低能 N+ 离子束为诱变源 ,黑腹果蝇为受试动物 ,检测 Am 1对离子束诱变果蝇的恢复功能。实验结果经统计学分析表明 ,1.0 %和 1.5 %浓度的蜜环菌多糖 Am 1可以提高受...水提法从蜜环菌 Am 99- 1菌株人工发酵菌体分离得到蜜环菌多糖 Am 1,以低能 N+ 离子束为诱变源 ,黑腹果蝇为受试动物 ,检测 Am 1对离子束诱变果蝇的恢复功能。实验结果经统计学分析表明 ,1.0 %和 1.5 %浓度的蜜环菌多糖 Am 1可以提高受试果蝇蛹的孵出率和降低当代及 F2代果蝇突变率。这说明蜜环菌多糖对于低能离子束诱变有一定程度的防护作用。展开更多
生长素影响了植物生长发育的诸多过程。生长素结合蛋白ABP1(auxin binding protein)作为一种生长素受体,在质膜上生长素诱导的快速反应中起重要作用。小麦中已经克隆获得了TaABP1-D,但其在细胞中的作用位置以及在染色体的定位情况仍不...生长素影响了植物生长发育的诸多过程。生长素结合蛋白ABP1(auxin binding protein)作为一种生长素受体,在质膜上生长素诱导的快速反应中起重要作用。小麦中已经克隆获得了TaABP1-D,但其在细胞中的作用位置以及在染色体的定位情况仍不明确。本研究利用洋葱表皮细胞瞬时表达系统对小麦生长素结合基因TaABP1-D进行亚细胞定位,结果表明TaABP1-D蛋白为膜蛋白,存在于细胞质和细胞膜中;同时利用中国春缺体-四体材料和信息学方法,将TaABP1-D定位在小麦5D染色体长臂的近着丝粒位置上,距两侧EST标记BE490079和BE405060的遗传距离分别为0.51 c M和0.28 c M。展开更多
基金supported by the National Natural Science Foundation of China(32172503 and 32260721)the Natural Science Foundation of Fujian Province,China(2023J01069)+2 种基金the State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,China(SKL2022001)the Innovation Fund of Fujan Agriculture and Forestry University,China(KFB23014A)the Undergraduate Training Program for Innovation and Entrepreneurship of Fujian Province,China(S202210389101).
文摘Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg hatching.Subsequent CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be achieved.However,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive capacity.We further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/Cas9.The results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous sequences.We ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in P.xylostella,which can also be used for early sex detection.By injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 gene.Taken together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in P.xylostella,while the establishment of a heritable strain required the positioning of appropriate sites.This study provides an important working basis and technical support for further developing genetic strategies for insect pest control.
文摘为了实现柔性作业车间完工时间、机器负荷、交货延期时间、车间能耗等多个目标的联合优化,提出了基于自适应惩罚MOEA/D(multi-objective evolutionary algorithm based on decomposition)的柔性车间多目标调度方法。对具有多个生产机床、多个加工任务、多道工序的柔性车间调度问题进行了描述并建立了优化模型。给出了基于MOEA/D算法的柔性车间调度方法,针对常值惩罚因子无法满足不同邻域对收敛性和染色体多样性不同调整需求的问题,提出了能够随邻域染色体密度自适应调整的惩罚因子,并制定了基于自适应惩罚MOEA/D算法的柔性车间调度流程。在具有8个机床、8个工件共28道工序的生产调度实验中,自适应MOEA/D算法搜索的Pareto前沿解能够支配标准MOEA/D和改进NSGA-Ⅱ算法的Pareto前沿解;在等权重最优解的生产实验中,自适应MOEA/D算法调度方案的完工时间、机器负荷、交货延期时间、车间能耗均小于标准MOEA/D算法和改进NSGA-Ⅱ算法。实验结果证明了自适应惩罚MOEA/D算法在柔性车间调度中的有效性和优越性。
文摘水提法从蜜环菌 Am 99- 1菌株人工发酵菌体分离得到蜜环菌多糖 Am 1,以低能 N+ 离子束为诱变源 ,黑腹果蝇为受试动物 ,检测 Am 1对离子束诱变果蝇的恢复功能。实验结果经统计学分析表明 ,1.0 %和 1.5 %浓度的蜜环菌多糖 Am 1可以提高受试果蝇蛹的孵出率和降低当代及 F2代果蝇突变率。这说明蜜环菌多糖对于低能离子束诱变有一定程度的防护作用。
文摘生长素影响了植物生长发育的诸多过程。生长素结合蛋白ABP1(auxin binding protein)作为一种生长素受体,在质膜上生长素诱导的快速反应中起重要作用。小麦中已经克隆获得了TaABP1-D,但其在细胞中的作用位置以及在染色体的定位情况仍不明确。本研究利用洋葱表皮细胞瞬时表达系统对小麦生长素结合基因TaABP1-D进行亚细胞定位,结果表明TaABP1-D蛋白为膜蛋白,存在于细胞质和细胞膜中;同时利用中国春缺体-四体材料和信息学方法,将TaABP1-D定位在小麦5D染色体长臂的近着丝粒位置上,距两侧EST标记BE490079和BE405060的遗传距离分别为0.51 c M和0.28 c M。
