Carbon-ion irradiation is a technique for trait improvement in the microalgae,but the underlying mechanisms that how it altered the biomass,and photosynthetic pigments accumulation were unclear.One mutant(DS240G-1)was...Carbon-ion irradiation is a technique for trait improvement in the microalgae,but the underlying mechanisms that how it altered the biomass,and photosynthetic pigments accumulation were unclear.One mutant(DS240G-1)was obtained from Dunaliella salina by heavy ion irradiation mutagenesis.Compared to the wild type,the biomass accumulation and maximum growth rate of DS240G-1 were increased by 34%and 55%respectively,and itsβ-carotene content was 21%higher than the wild type.Subsequent analysis of the chlorophyll fluorescence parameters indicated that higherβ-carotene productivity was likely owing to the improved maximum quantum efficiency(Fv/Fm)and decreased thermal dissipation of photosynthesis in DS240G-1 than that of wild type during cultivation.In addition,the result of this study revealed that high content of ROS may induceβ-carotene accumulation in mutant DS240G-1.Also,the total fatty acid(TFA)content in mutant DS240G-1 was 79%higher than that in wild type.Owing to its highβ-carotene productivity and total fatty acid content,DS240G-1 could be considered as a promising candidate for microalgaeβ-carotene and biodiesel production.This work provided the first insight into the biological effects involved in carbon-ions irradiation on the photosynthetic activity of D.salina.展开更多
基金This work was supported by the National Nature Science Foundation of China(Grant No.11665011)Gansu Provincial Science and Technology Program(Grant No.18JR2JG001).
文摘Carbon-ion irradiation is a technique for trait improvement in the microalgae,but the underlying mechanisms that how it altered the biomass,and photosynthetic pigments accumulation were unclear.One mutant(DS240G-1)was obtained from Dunaliella salina by heavy ion irradiation mutagenesis.Compared to the wild type,the biomass accumulation and maximum growth rate of DS240G-1 were increased by 34%and 55%respectively,and itsβ-carotene content was 21%higher than the wild type.Subsequent analysis of the chlorophyll fluorescence parameters indicated that higherβ-carotene productivity was likely owing to the improved maximum quantum efficiency(Fv/Fm)and decreased thermal dissipation of photosynthesis in DS240G-1 than that of wild type during cultivation.In addition,the result of this study revealed that high content of ROS may induceβ-carotene accumulation in mutant DS240G-1.Also,the total fatty acid(TFA)content in mutant DS240G-1 was 79%higher than that in wild type.Owing to its highβ-carotene productivity and total fatty acid content,DS240G-1 could be considered as a promising candidate for microalgaeβ-carotene and biodiesel production.This work provided the first insight into the biological effects involved in carbon-ions irradiation on the photosynthetic activity of D.salina.
