D152树脂预洗工艺对大观霉素质量的影响

大观霉素(Spectinomycin)是一种碱性水溶性氨基糖苷类抗生素,由壮观链霉(Actinospectacin)发酵产生。在用D152树脂提纯大观霉素过程中,引入EDTA-2Na预洗工艺,通过选择合适的预洗液浓度、pH和流速,实现高纯度盐酸大观霉素的提取,并确定了EDTA-2Na的回收工艺。研究得到了最佳预洗工艺参数:EDTA-2Na洗液浓度为0.015 mol/L,预洗液pH为7.5~8.0,流速为1.0~2.0 BV/h。该工艺简便易行、成本低、回收率高,保证了D152树脂的吸附收率,实现了绿色提取。

D152树脂对镝的吸附及机制

对D152树脂吸附镝(Ⅲ)的过程及镝在D152树脂上的吸附行为进行了研究。研究了介质pH、温度、吸附时间、树脂量等因素对吸附过程的影响。实验结果表明:D152树脂对镝(Ⅲ)的吸附在pH=5.93的HAc-NaAc的缓冲溶液中为最佳。25℃时静态饱和吸附量为314.6mg.g-1(干树脂)。用1.0 mol.L-1的HCl溶液作为解吸剂,解吸率为96.2%;D152树脂对镝(Ⅲ)的表观吸附活化能Ea=18.28 kJ.mol-1,表观吸附速率常数k298=2.54×10-5s-1,k308=2.93×10-5s-1,k318=4.05×10-5s-1,测得热力学参数分别为ΔH=18.19 kJ.mol-1,ΔG=-0.88 kJ.mol-1,ΔS=63.99 J.mol-1.K-1,等温吸附服从Freundlich经验式。并用化学和红外光谱的方法探求树脂对镝(Ⅲ)的吸附机制。

D152树脂对铕(Ⅲ)的吸附作用

研究了D152树脂在NaAc-HAc缓冲体系中对Eu^3＋的吸附与解吸行为。结果表明：静态饱和吸附容量为340mg／g；用0．1～2．0mol／L HCl洗脱，洗脱率为92％以上；测得吸附热力学函数分别为：AH=14．3kJ／mol、△G298=-1．88kJ／mol、△S=54．3J／（mol·K）；等温吸附曲线遵守Freundlich关系式；表观活化能Ea=9．10kJ／mol，表观速率常数k298=1．54×10^-5s^-1。用化学和红外光谱的方法探讨了树脂对Eu^3＋的吸附机理。

D152树脂对铜(Ⅱ)的吸附性能

The sorption behavior and mechanism of D152 resin for Cu2+is investigated.D152 resin has a good adsorptivity for Cu2+in the HAc—NaAc medium at pH=4.73.The statically saturated sorption capacity is 309mg/g·(resin).Cu2+adsorbed on D152 resin can be eluted quantitatively by using 0.1-2.0mol/L hydrochloric acid as an eluant.The apparent rate constant and activation energy are k298=1.86×10-5 sec-1 and Ea=12.3kJ/mol.The sorption behavior of D152 resin for Cu2+ obeys the Freundlich isotherm.The sorption thermodynamic parameters of D152 resin for Cu2+are enthalpy change ΔH=14.6 kJ/mol,free energy change ΔG=-0.72kJ/mol,entropy change ΔS=51.4J/(.mol·K).respectively.

Effect of pH on sorption for RE(III) and sorption behaviors of Sm(III) by D152 resin

The pH dependent sorption of rare earth ions （La（Ⅲ）, Ce（Ⅲ）, Pr（Ⅲ）, Nd（Ⅲ）, Y（Ⅲ）, Sm（Ⅲ）, Eu（Ⅲ）, Gd（Ⅲ）, Tb（Ⅲ）, Dy（Ⅲ）, Ho（Ⅲ）, Er（Ⅲ）, Lu（Ⅲ）, and Yb（Ⅲ）） from HAC-NaAC buffer solution at 298 K by D152 resin containing -COOH function groups were presented. The sorption behaviors of D152 resin for Sm（Ⅲ） were discussed as an example. The effects of operational conditions such as pH, temperature, and contact time were studied. The statically saturated sorption capacity was 510 mg/g resin at pH 6.70 in HAc-NaAc medium at 298 K. The sorption behaviors obeyed the Freundlich isotherm. The capacity value for column study was obtained by graphical integration as 495 mg/g resin. Thomas model was applied to experimental data to predict the breakthrough curve and to determine the characteristic parameters of the column useful for process design.

Sorption Behavior of D152 Resin for Lanthanum

The sorption and desorption properties of La(Ⅲ) on D152 resin were investigated. The influence of operational conditions such as La(Ⅲ) initial concentration, sorbent mass, temperature, solution initial pH and sorption kinetics of La(Ⅲ) were studied. The optimal pH for the sorption was found to be 6.70 in the HAc-NaAc system. The statically saturated sorption capacity is 463 mg·g-1 at 298 K in HAc-NaAc medium. The static sorption data fit to the Freundlich isotherm and Langmiur isotherm. The sorption rate constant is k298 K=1.73×10-5 s-1. The thermodynamic sorption parameters, enthalpy change ΔH, entropy change ΔS and free energy change ΔG298 K of D152 resin for La(Ⅲ) are 15.9 kJ·mol-1, 132 J·K-1·mol-1, -39.4 kJ·mol-1, respectively. Dynamic sorption and desorption experiments have been carried out with the column packed by D152 resin. La(Ⅲ) adsorbed on D152 resin can be quantificationally eluted and the elution percentage is up to 100%.

D152树脂对多粘菌素E的提取性能研究

多粘菌素E由多粘类芽孢杆菌发酵产生,主要采用离子交换法进行提纯。研究了D152阳离子交换树脂提取多粘菌素E的各项指标,并将D152树脂与现有工艺设备进行匹配,得到了提取过程中吸附量、洗脱速率、收率等指标。多粘菌素E过滤液pH为6.0~7.0,色谱效价30万U/mL,采用3柱串联吸附工艺,在吸附流速1.0 BV/h、硫酸洗脱流速1.0~1.5 BV/h条件下,D152树脂吸附量可达680万U/mL(湿树脂),总收率≥98.0%。连续运行6个月,D152树脂性能稳定。

W/D152催化剂的制备及其对汽油中二苯并噻吩的催化氧化性能

采用浸渍法将钨负载在丙烯酸系弱酸性阳离子交换树脂D152上,制成了催化剂W/D152.实验研究了浸渍钨溶液p H值、温度、浓度等制备条件对催化氧化去除二苯并噻吩(DBT)活性的影响,并确定了催化剂的最佳制备条件.同时,运用红外光谱(FTIR)和环境电镜扫描(SEM)对W/D152的微观面貌进行了观察和分析.结果表明,在p H值为2.5,温度为30℃,2.5%(质量分数)的含钨溶液条件下制备的催化剂脱硫效果最佳;初始溶液含硫量为400 mg·kg-1,最高去除率达到99.1%,处理后样品的含硫量约为3.52 mg·kg-1,达到了总含硫量要求小于10mg·kg-1的欧五标准.催化剂表征结果表明,钨成功负载在树脂上,并形成一层薄层.W/D152催化剂循环使用7次后,处理后样品的含硫量仍可达到欧五标准.该催化剂催化氧化二苯并噻吩的反应符合一级动力学模型,速率常数为0.1091 min-1.

除镁剂的筛选及热力学研究

以树脂对镁离子的吸附量为评价指标,采用静态吸附筛选法,从001×1.1、001×14.5、C800、D401、D152 5种树脂中筛选性能优良的树脂.通过正交实验法确定树脂除镁的最佳工艺条件,并对吸附过程进行热力学方面的研究.实验结果表明:大孔树脂D152可以有效去除溶液中镁离子;通过正交实验法确定在pH=6,温度为25℃,D152树脂在溶液中的最佳投加量为37.5 g L1,吸附量达到17.86 mg g1;Langmuir等温线方程能更好的拟合吸附数据,计算出吸附过程热力学参数H=0.82 kJ mol1、S=20.2 J mol K1,表明该吸附反应是吸热、熵增的过程.