In order to study the molecular mechanism of the inhibitory effect of 1,25-dihydroxyvitamin D3 on dendritic cells, experiments were performed using Atlas cDNA expression arrays from Clonetech to identify the different...In order to study the molecular mechanism of the inhibitory effect of 1,25-dihydroxyvitamin D3 on dendritic cells, experiments were performed using Atlas cDNA expression arrays from Clonetech to identify the differentially expressed genes of dendritic cells by 1,25-dihydroxyvitamin D3. Analysis of cDNA arrays revealed changes in the expression of 9 genes, including those involved in DNA binding and transcription, extracellular cell signaling and communication, intracellular transducers, as well as cell adhesions. The results indicated that a multiple molecular network is involved in the inhibitory role of 1,25-dihydroxyvitamin D3 on dendritic cells. The Atlas Array technology may facilitate the elucidation of complex pharmacological nrocess of 1.25-dihvdroxvvitamin D3 on dondritie cells.展开更多
均衡的体成分构成对维持机体的健康状态具有重要作用,体成分受遗传与环境因素的共同影响。多巴胺参与摄食、运动及认知等活动的调节,多巴胺D3受体(DRD3)对多巴胺神经通路起关键调节作用,进而对摄食功能发挥作用,从而可能对体成分产生影...均衡的体成分构成对维持机体的健康状态具有重要作用,体成分受遗传与环境因素的共同影响。多巴胺参与摄食、运动及认知等活动的调节,多巴胺D3受体(DRD3)对多巴胺神经通路起关键调节作用,进而对摄食功能发挥作用,从而可能对体成分产生影响。为了解遗传与环境因素对双生子儿童体成分的影响,并探讨DRD3基因单核苷酸多态性(SNP)与体成分的相关性,对160对4-12岁双生子肱三头肌皮褶厚度(d_(1))、肩胛下皮褶厚度(d_(2))、髂前上棘位皮褶厚度(d_(3))和体质量(m)进行了测量,计算d_(4)(d_(1)+d_(2))、d_(5)(d_(2)/d_(1)),体脂率(P_(f))、瘦体质量(m_(l));从口腔拭子中提取全基因组DNA;通过Amp FISTR Sino filerPlus试剂盒分析确定卵型;采用SNaPshot技术对DRD3基因4个SNP位点进行检测;使用Mx软件估算各指标遗传度;运用广义估计方程模型分析各指标与DRD3基因SNP的相关性。校正年龄效应后,除个别指标(d_(3),m_(l))外,男女生指标遗传度(h)学龄前期总体偏低,且某些指标(d_(2),d_(4),P_(f),m_(l))的遗传度存在一定的性别差异。d_(2)分别与rs324029、rs226082存在相关(P<0.05);d_(3)分别与rs2134655、rs226082存在相关(P<0.05);d_(5)分别与rs2134655、rs167771存在相关(P<0.05);P_(f)分别与rs226082、rs167771存在相关(P<0.05);m_(l)分别与rs2134655、rs226082、rs167771存在相关(P<0.05)。本研究结果表明,遗传和环境因素对儿童体成分发育均有影响,但遗传效应可能存在一定的发育阶段和性别差异;DRD3基因SNPs与儿童的体成分可能存在一定的相关性。展开更多
文摘In order to study the molecular mechanism of the inhibitory effect of 1,25-dihydroxyvitamin D3 on dendritic cells, experiments were performed using Atlas cDNA expression arrays from Clonetech to identify the differentially expressed genes of dendritic cells by 1,25-dihydroxyvitamin D3. Analysis of cDNA arrays revealed changes in the expression of 9 genes, including those involved in DNA binding and transcription, extracellular cell signaling and communication, intracellular transducers, as well as cell adhesions. The results indicated that a multiple molecular network is involved in the inhibitory role of 1,25-dihydroxyvitamin D3 on dendritic cells. The Atlas Array technology may facilitate the elucidation of complex pharmacological nrocess of 1.25-dihvdroxvvitamin D3 on dondritie cells.
文摘均衡的体成分构成对维持机体的健康状态具有重要作用,体成分受遗传与环境因素的共同影响。多巴胺参与摄食、运动及认知等活动的调节,多巴胺D3受体(DRD3)对多巴胺神经通路起关键调节作用,进而对摄食功能发挥作用,从而可能对体成分产生影响。为了解遗传与环境因素对双生子儿童体成分的影响,并探讨DRD3基因单核苷酸多态性(SNP)与体成分的相关性,对160对4-12岁双生子肱三头肌皮褶厚度(d_(1))、肩胛下皮褶厚度(d_(2))、髂前上棘位皮褶厚度(d_(3))和体质量(m)进行了测量,计算d_(4)(d_(1)+d_(2))、d_(5)(d_(2)/d_(1)),体脂率(P_(f))、瘦体质量(m_(l));从口腔拭子中提取全基因组DNA;通过Amp FISTR Sino filerPlus试剂盒分析确定卵型;采用SNaPshot技术对DRD3基因4个SNP位点进行检测;使用Mx软件估算各指标遗传度;运用广义估计方程模型分析各指标与DRD3基因SNP的相关性。校正年龄效应后,除个别指标(d_(3),m_(l))外,男女生指标遗传度(h)学龄前期总体偏低,且某些指标(d_(2),d_(4),P_(f),m_(l))的遗传度存在一定的性别差异。d_(2)分别与rs324029、rs226082存在相关(P<0.05);d_(3)分别与rs2134655、rs226082存在相关(P<0.05);d_(5)分别与rs2134655、rs167771存在相关(P<0.05);P_(f)分别与rs226082、rs167771存在相关(P<0.05);m_(l)分别与rs2134655、rs226082、rs167771存在相关(P<0.05)。本研究结果表明,遗传和环境因素对儿童体成分发育均有影响,但遗传效应可能存在一定的发育阶段和性别差异;DRD3基因SNPs与儿童的体成分可能存在一定的相关性。
