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DAPI检测黄体期绵羊生殖器官中细胞凋亡的比较研究 被引量:3
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作者 葛晨霞 王龙涛 +2 位作者 李向军 贾博寅 姜怀志 《中国畜牧杂志》 CAS 北大核心 2011年第3期27-29,共3页
以黄体期小尾寒羊和萨福克羊为研究对象,采用DAPI原位荧光标记技术,对高繁殖力品种小尾寒羊和低繁殖力品种萨福克羊成年母羊主要生殖器官(卵巢、输卵管、子宫)进行细胞凋亡的确认与定位。结果表明:小尾寒羊和萨福克羊卵巢中黄体细胞有... 以黄体期小尾寒羊和萨福克羊为研究对象,采用DAPI原位荧光标记技术,对高繁殖力品种小尾寒羊和低繁殖力品种萨福克羊成年母羊主要生殖器官(卵巢、输卵管、子宫)进行细胞凋亡的确认与定位。结果表明:小尾寒羊和萨福克羊卵巢中黄体细胞有凋亡现象,黄体后期萨福克羊的细胞凋亡较小尾寒羊明显;在萨福克羊黄体期输卵管各部位上皮中发现有极少数分泌细胞具有凋亡特征,而在小尾寒羊未检测到凋亡细胞,两品种羊输卵管上皮下结缔组织有少数凋亡细胞,小尾寒羊和萨福克羊子宫内膜的基质与腺体内皮中均检测到大量凋亡细胞。细胞凋亡是母羊生殖器官变化的重要调节机制,可能与造成两者繁殖力高低差异有关。 展开更多
关键词 黄体期 dapi检测 细胞凋亡 小尾寒羊 萨福克羊
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DAPI检测卵泡期绵羊生殖器官中细胞凋亡的比较研究 被引量:1
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作者 葛晨霞 王龙涛 +2 位作者 李向军 王欣睿 姜怀志 《中国农业大学学报》 CAS CSCD 北大核心 2011年第2期109-113,共5页
为探讨在卵泡期绵羊生殖器官中细胞凋亡的作用机制,本试验以小尾寒羊和萨福克羊为研究对象,采用DAPI(4′,6′-联脒-2-苯基吲哚)原位荧光标记技术,对高繁殖力品种小尾寒羊和低繁殖力品种萨福克羊成年母羊卵泡期主要生殖器官(卵巢、输卵... 为探讨在卵泡期绵羊生殖器官中细胞凋亡的作用机制,本试验以小尾寒羊和萨福克羊为研究对象,采用DAPI(4′,6′-联脒-2-苯基吲哚)原位荧光标记技术,对高繁殖力品种小尾寒羊和低繁殖力品种萨福克羊成年母羊卵泡期主要生殖器官(卵巢、输卵管、子宫)进行细胞凋亡的确认与定位。结果显示:小尾寒羊和萨福克羊卵巢中卵泡上皮细胞、颗粒细胞、膜细胞均有凋亡现象。在三级卵泡和成熟卵泡的颗粒细胞和膜细胞中,萨福克羊比小尾寒羊表达出较多数量的凋亡细胞,两品种羊在卵泡期输卵管各部,子宫内膜腺体中几乎均未检测到凋亡细胞,子宫内膜基质中检测到少量细胞发生凋亡。因此,研究表明细胞凋亡是母羊生殖器官变化的重要调节机制,可能与造成两者繁殖力高低差异有关。 展开更多
关键词 绵羊 卵泡期 dapi检测 细胞凋亡
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海南长春花黄化病植原体的DAPI荧光显微检测 被引量:3
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作者 陶明福 车海彦 罗大全 《热带农业工程》 2010年第2期17-20,共4页
对感染黄化病的海南长春花植株,应用DAPI荧光显微技术对其嫩茎和叶柄进行了切片染色观察,结果表明:在感病植株的韧皮部筛管分子中存在大量特异性的植原体DNA荧光光点,而在健康植株的筛管分子则未发现有特异性的植原体荧光。另对其茎和... 对感染黄化病的海南长春花植株,应用DAPI荧光显微技术对其嫩茎和叶柄进行了切片染色观察,结果表明:在感病植株的韧皮部筛管分子中存在大量特异性的植原体DNA荧光光点,而在健康植株的筛管分子则未发现有特异性的植原体荧光。另对其茎和叶柄中的植原体含量进行比较,结果显示茎中的含量要比叶柄中稍高。 展开更多
关键词 长春花黄化病 植原体 dapi检测
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Assesment of Frequency-Magnitude of Extreme Rainfall Events-Case Study of the MeKong River Delta
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作者 Pham Hai An Tran Anh Tu +1 位作者 Tran Dinh Lan Nguyen Ngoc Tien 《Journal of Environmental Science and Engineering(B)》 2015年第3期161-168,共8页
Extreme rainfall events are primary natural hazards, which cause a severe threat to people and their properties in populated cities, which are normally located in coastal areas in Vietnam. Analysing these events by us... Extreme rainfall events are primary natural hazards, which cause a severe threat to people and their properties in populated cities, which are normally located in coastal areas in Vietnam. Analysing these events by using a data series observed over years will support us to draw a picture of how the climate change impact on local environments. The purpose of this report is to understand the characteristics of the extreme rainfall events in MEKONG river delta (south VietNam). Daily rainfall data in the period of 30 years for a meteorological station in each area were collected from the Vietnam National Hydro-meteorological Service. The extreme rainfall events were defined as those exceeding the 95th percentile for each station. The analytical results show that the rainfall values (95th percentile) are 37.4 mm/day at Nam Can station, 27 mm/day at My Thanh station, 22.4 mm/day at Hoa Binh station, 23.8 mm/day at Binh Dai station and 22.7 mm/day at Ben Trai station. The highest rainfall data ever recorded are 246.4 mm/day (Nam Can), 174.5 mm/day (My Thanh), 179 mm/day (Hoa Bin_h), 187.3 mm/day (Binh Dai) and 136.3 mm/day (Ben Trai) during 1983-2012. The result of the Mann-Kendall tests show that there was a significant creasing of the rainfall at Nam Can, My Thanh station in two periods (1983-2012, 1998-2012) while no clear trend of the rainfall was recoreded at Hoa Birth, Binh Dai, Ben Trai station. In order to estimate the return period of the extreme rainfall events, the method General Extreme Value Distribution was used to calculate frequent distribution. The magnitudes of daily maximum rainfall were from 2 to 100 years. The results of return period show that maximum rainfalls are 46.6 mm at Nam Can station (highest) and 31.4 mm at Hoa Birth station (lowest) during 50 years. Similarly, maximum rainfalls are expected to be about 55.1 mm at Nam Can station and 37.2 mm at Hoa Birth station for 100 years. 展开更多
关键词 Generalized extreme value distribution MEKONG river delta.
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