目的观察补肾强督方对自发性强直性脊柱炎模型DBA/1小鼠关节韧带及附着点骨化程度和DKK1/Wnt通路的影响,探讨补肾强督方防治强直性脊柱炎的作用机制。方法将30只12周龄的雄性DBA/1小鼠随机分为模型组、阳性药物组、补肾强督方低、中、...目的观察补肾强督方对自发性强直性脊柱炎模型DBA/1小鼠关节韧带及附着点骨化程度和DKK1/Wnt通路的影响,探讨补肾强督方防治强直性脊柱炎的作用机制。方法将30只12周龄的雄性DBA/1小鼠随机分为模型组、阳性药物组、补肾强督方低、中、高剂量组,每组6只,另设6只同周龄C57BL/6小鼠作为空白组。补肾强督方低、中及高剂量组分别灌胃低、中、高浓度的补肾强督方,所含生药重量分别为11.25、22.5、45 g/(kg·d),0.2 m L/只,每日1次;阳性药物组灌胃塞来昔布胶囊0.8 mg/只,0.2 m L/只,每日1次;模型组及空白组灌胃等量的生理盐水。连续饲养、灌胃12周。定期观察小鼠体重、饮食、大便、毛发等情况。每两周评价1次小鼠关节炎体征。处死后取小鼠跟腱部位进行大体观察,对跟腱组织行HE染色,用免疫组化法检测小鼠跟腱中碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(bone gamma-carboxyglutamicacid-containing proteins,BGP)、DKK1、Wnt5a的蛋白表达。结果与空白对照组比较,模型组关节炎体征评分明显升高,而补肾强督方各剂量组及阳性药物组评分均低于模型组(P<0.05)。跟腱组织病理观察显示,正常组无炎性细胞及成纤维细胞浸润,组织形态结构正常;模型组出现不同程度的软骨及骨形成、炎性细胞及附着点成纤维样细胞浸润;各给药组小鼠跟腱组织多见散在淋巴细胞浸润,软骨及骨形成较少见。与空白组比较,模型组组织标本骨化评分升高,补肾强督方各剂量组及阳性药物组评分均低于模型组,差异有统计学意义(P<0.05)。与空白组比较,模型组DKK1蛋白表达降低,Wnt5a蛋白表达升高(P<0.05);与模型组比较,补肾强督方高、中剂量组DKK1蛋白表达升高,Wnt5a蛋白降低,差异有统计学意义(P<0.05)。结论补肾强督方可能通过抑制经典Wnt通路来延缓自发性强直性脊柱炎模型DBA/1小鼠关节炎及骨化程度的发生与发展。展开更多
癫痫猝死(sudden unexpected death in epilepsy,SUDEP)是癫痫患者在发作期或发作间期突发的无法解释的死亡,其风险明显高于普通人群。其可能是多种机制共同作用的结果,包括呼吸抑制、致命性心律失常、自主功能障碍等。但发病机制仍未...癫痫猝死(sudden unexpected death in epilepsy,SUDEP)是癫痫患者在发作期或发作间期突发的无法解释的死亡,其风险明显高于普通人群。其可能是多种机制共同作用的结果,包括呼吸抑制、致命性心律失常、自主功能障碍等。但发病机制仍未能完全阐明,为更深入、客观、科学的认识,进行SUDEP模型研究。文中对SUDEP模型包括DBA模型、Dravet综合征模型、Kv1.1型钾通道模型、家族性长QT综合征模型等研究进行综述。展开更多
Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific in...Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific investigations have attempted to validate this impression. The ingredients of the MD include significant amounts of omega (ω3, ω6, and ω9) unsaturated fatty acids (UFAs). A few studies of these UFAs in the prevention or treatment of arthritis have yielded controversial results, but a general belief regarding their beneficial effects has prevailed. Objective: To investigate the effects of three relevant UFAs, namely Docosahexaenoic Acid (DHA), Arachidonic Acid (AA), and Oleic Acid (OA) (ω3, ω6, and ω9, respectively), in the development of arthritis using a murine model of Collagen-Induced Arthritis (CIA). Methods: DBA-1 mice were immunized with chicken collagen type II (CII) and were subsequently treated with ω-UFAs for 53 days. Dexamethasone (DEXA) was used as a positive anti-inflammatory agent. The effect of the treatments was evaluated through several parameters: inflammation indices, antibody levels, cell prolifera- tion, and histopathological findings. Results and Conclusion: The anti-inflammatory effect of the tested substances was inversely correlated with the histopathological findings: a greater anti- inflammatory effect was associated with less articular damage. Oleic acid (ω9) was the most efficient anti-inflammatory UFA, followed by DHA and then AA. DEXA completely inhibited the development of arthritis, whereas the untreated CII-immunized mice developed the most severe articular damage. DBA-1 mice with CII-induced arthritis constitute an adequate model for the study of arthritis and its treatment.展开更多
文摘目的观察补肾强督方对自发性强直性脊柱炎模型DBA/1小鼠关节韧带及附着点骨化程度和DKK1/Wnt通路的影响,探讨补肾强督方防治强直性脊柱炎的作用机制。方法将30只12周龄的雄性DBA/1小鼠随机分为模型组、阳性药物组、补肾强督方低、中、高剂量组,每组6只,另设6只同周龄C57BL/6小鼠作为空白组。补肾强督方低、中及高剂量组分别灌胃低、中、高浓度的补肾强督方,所含生药重量分别为11.25、22.5、45 g/(kg·d),0.2 m L/只,每日1次;阳性药物组灌胃塞来昔布胶囊0.8 mg/只,0.2 m L/只,每日1次;模型组及空白组灌胃等量的生理盐水。连续饲养、灌胃12周。定期观察小鼠体重、饮食、大便、毛发等情况。每两周评价1次小鼠关节炎体征。处死后取小鼠跟腱部位进行大体观察,对跟腱组织行HE染色,用免疫组化法检测小鼠跟腱中碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(bone gamma-carboxyglutamicacid-containing proteins,BGP)、DKK1、Wnt5a的蛋白表达。结果与空白对照组比较,模型组关节炎体征评分明显升高,而补肾强督方各剂量组及阳性药物组评分均低于模型组(P<0.05)。跟腱组织病理观察显示,正常组无炎性细胞及成纤维细胞浸润,组织形态结构正常;模型组出现不同程度的软骨及骨形成、炎性细胞及附着点成纤维样细胞浸润;各给药组小鼠跟腱组织多见散在淋巴细胞浸润,软骨及骨形成较少见。与空白组比较,模型组组织标本骨化评分升高,补肾强督方各剂量组及阳性药物组评分均低于模型组,差异有统计学意义(P<0.05)。与空白组比较,模型组DKK1蛋白表达降低,Wnt5a蛋白表达升高(P<0.05);与模型组比较,补肾强督方高、中剂量组DKK1蛋白表达升高,Wnt5a蛋白降低,差异有统计学意义(P<0.05)。结论补肾强督方可能通过抑制经典Wnt通路来延缓自发性强直性脊柱炎模型DBA/1小鼠关节炎及骨化程度的发生与发展。
文摘癫痫猝死(sudden unexpected death in epilepsy,SUDEP)是癫痫患者在发作期或发作间期突发的无法解释的死亡,其风险明显高于普通人群。其可能是多种机制共同作用的结果,包括呼吸抑制、致命性心律失常、自主功能障碍等。但发病机制仍未能完全阐明,为更深入、客观、科学的认识,进行SUDEP模型研究。文中对SUDEP模型包括DBA模型、Dravet综合征模型、Kv1.1型钾通道模型、家族性长QT综合征模型等研究进行综述。
文摘Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific investigations have attempted to validate this impression. The ingredients of the MD include significant amounts of omega (ω3, ω6, and ω9) unsaturated fatty acids (UFAs). A few studies of these UFAs in the prevention or treatment of arthritis have yielded controversial results, but a general belief regarding their beneficial effects has prevailed. Objective: To investigate the effects of three relevant UFAs, namely Docosahexaenoic Acid (DHA), Arachidonic Acid (AA), and Oleic Acid (OA) (ω3, ω6, and ω9, respectively), in the development of arthritis using a murine model of Collagen-Induced Arthritis (CIA). Methods: DBA-1 mice were immunized with chicken collagen type II (CII) and were subsequently treated with ω-UFAs for 53 days. Dexamethasone (DEXA) was used as a positive anti-inflammatory agent. The effect of the treatments was evaluated through several parameters: inflammation indices, antibody levels, cell prolifera- tion, and histopathological findings. Results and Conclusion: The anti-inflammatory effect of the tested substances was inversely correlated with the histopathological findings: a greater anti- inflammatory effect was associated with less articular damage. Oleic acid (ω9) was the most efficient anti-inflammatory UFA, followed by DHA and then AA. DEXA completely inhibited the development of arthritis, whereas the untreated CII-immunized mice developed the most severe articular damage. DBA-1 mice with CII-induced arthritis constitute an adequate model for the study of arthritis and its treatment.
