Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936

The fall armyworm(FAW)Spodoptera frugiperda,which originated in the Americas,is advancing across China and threatening the nation’s maize crops.Currently,one widely used tool for its control is genetically modified(GM)Bacillus thuringiensis(Bt)maize.Sufficient content of Bt protein in appropriate plant parts is crucial for enhancing resistance against insect pests.In this study,we conducted a systematic investigation of Cry1 Ab levels in Chinese domestic GM maize DBN9936,which has recently obtained a biosafety certificate,and evaluated its efficacy against FAW.Quantification of expression levels of Cry1 Ab,via ELISA,indicated a spatio-temporal dynamic,with significant variation of mean Cry1 Ab,ranging from 0.76 to 8.48μg g-1 FW with the Cry1 Ab protein level ranked as:V6-V8 leaf>R1 leaf>R4 leaf>R1 silk>VT tassel>R4 kernel.Among the nine locations,the Cry1 Ab levels in DBN9936 of the Xinxiang,Langfang,and Harbin fields were significantly lower than those from Wuhan and Shenyang,and were slightly,but not significantly lower than those from the other four fields.Furthermore,the artificial diet-Cry1 Ab mixture and plant tissue feeding bioassays revealed that DBN9936 has high efficacy against FAW.The insecticidal efficacy of different tissues against FAW larvae reached 34-100%with a descending order of lethality as follows:VT leaf>R4 leaf>R1 husk>R1 silk>VT tassel>R4 kernel.Taken together,our results showed that Bt-Cry1 Ab maize DBN9936 has potential as a promising strategy to manage FAW.

草甘膦施用量对转基因玉米杂草防效、产量以及CP4-EPSPS合酶表达的影响

为了解草甘膦施加量对种植于内蒙古农牧业科学院生物育种科研试验基地的转基因玉米杂草防效、产量以及CP4-EPSPS合酶表达量的影响,本文对转基因玉米DBN9936系列远科105品种进行2250 mL/hm^(2)、3000 mL/hm^(2)和3750 mL/hm^(2)41%草甘膦处理,提取并测定远科105D品种中CP4-EPSPS蛋白的表达量,结合施药过程中的药害、除草效果、产量的测定等数据。研究结果表明:转基因玉米DBN9936系列远科105D品种中抗草甘膦蛋白CP4-EPSPS为组成型表达蛋白,在玉米的不同生育期内都能稳定表达,并不与草甘膦的施加量成正比。在喷洒草甘膦后的第45 d进行杂草统计:施加3750 mL/hm^(2)的41%草甘膦可以将田间94.8%的稗、藜、反枝苋、狗尾草、蒺藜等杂草清除掉,而施加3000 mL/hm^(2)的41%草甘膦除草率为93.1%,施加2250 mL/hm^(2)的草甘膦除草率为87.7%。产量数据表明,有效防控草害能提高玉米产量约16%,而不同草甘膦施药量对玉米的增产并不明显。综上,考虑到内蒙古中西部沙壤土中主要杂草的种类和数量,喷洒2250mL/hm^(2)的草甘膦是既节约成本,也能有效除草,并且也不会明显降低产量的一个合适的施药量。