The reciprocal activating crosstalk between dendritic cells （DCs） and natural killer （NK） cells plays a pivotal role in regulating immune defense against viruses and tumors. The cytokine-producing capacity, Th-cel...The reciprocal activating crosstalk between dendritic cells （DCs） and natural killer （NK） cells plays a pivotal role in regulating immune defense against viruses and tumors. The cytokine-producing capacity, Th-cell polarizing ability and chemokine expression, migration and stimulatory functions of DCs are regulated by activated NK cells. Conversely, the innate and effector functions of NK cells require close interactions with activated DCs. Cell membrane-associated molecules and soluble mediators, including cytokines and prostaglandins （PGs）, contribute to the bidirectional crosstalk between DCs and NK cells. One of the most well-known and well-studied PGs is PGE2. Produced by many cell types, PG E2 has been shown to affect various aspects of the immune and inflammatory responses by acting on all components of the immune system. There is emerging evidence that PGE2 plays crucial roles in DC and NK cell biology. Several studies have shown that DCs are not only a source of PGE2, but also a target of its immunomodulatory action in normal immune response and during immune disorders. Although NK cells appear to be unable to produce PGE2, they are described as powerful PGE2-responding cells, as they express all PGE2 E-prostanoid （EP） receptors. Several NK cell functions （lysis, migration, proliferation, cytokine production） are influenced by PGE2. This review highlights the effects of PGE2 on DC- NK cell crosstalk and its subsequent impact on immune regulations in normal and immunopathological processes.展开更多
目的观察细胞因子诱导的自体DC、CIK、NK、αβT、γδT细胞治疗组织细胞肉瘤疗效。方法抽取患者自体外周血60~100 m L，采用血细胞分离机收集其单核细胞，经细胞因子诱导并扩增C I K、N K、αβT、γδT细胞及DC细胞；用流式细胞仪检...目的观察细胞因子诱导的自体DC、CIK、NK、αβT、γδT细胞治疗组织细胞肉瘤疗效。方法抽取患者自体外周血60~100 m L，采用血细胞分离机收集其单核细胞，经细胞因子诱导并扩增C I K、N K、αβT、γδT细胞及DC细胞；用流式细胞仪检测其表型后，静脉回输CIK、NK、αβT、γδT细胞，皮下注射DC细胞，观察组织细胞肉瘤变化。结果患者左乳肿块缩小＞50%，生活质量明显改善。结论细胞免疫治疗是一种化疗后组织细胞肉瘤有效的治疗手段，具有广阔的临床应用前景。展开更多
文摘The reciprocal activating crosstalk between dendritic cells （DCs） and natural killer （NK） cells plays a pivotal role in regulating immune defense against viruses and tumors. The cytokine-producing capacity, Th-cell polarizing ability and chemokine expression, migration and stimulatory functions of DCs are regulated by activated NK cells. Conversely, the innate and effector functions of NK cells require close interactions with activated DCs. Cell membrane-associated molecules and soluble mediators, including cytokines and prostaglandins （PGs）, contribute to the bidirectional crosstalk between DCs and NK cells. One of the most well-known and well-studied PGs is PGE2. Produced by many cell types, PG E2 has been shown to affect various aspects of the immune and inflammatory responses by acting on all components of the immune system. There is emerging evidence that PGE2 plays crucial roles in DC and NK cell biology. Several studies have shown that DCs are not only a source of PGE2, but also a target of its immunomodulatory action in normal immune response and during immune disorders. Although NK cells appear to be unable to produce PGE2, they are described as powerful PGE2-responding cells, as they express all PGE2 E-prostanoid （EP） receptors. Several NK cell functions （lysis, migration, proliferation, cytokine production） are influenced by PGE2. This review highlights the effects of PGE2 on DC- NK cell crosstalk and its subsequent impact on immune regulations in normal and immunopathological processes.
文摘目的观察细胞因子诱导的自体DC、CIK、NK、αβT、γδT细胞治疗组织细胞肉瘤疗效。方法抽取患者自体外周血60~100 m L，采用血细胞分离机收集其单核细胞，经细胞因子诱导并扩增C I K、N K、αβT、γδT细胞及DC细胞；用流式细胞仪检测其表型后，静脉回输CIK、NK、αβT、γδT细胞，皮下注射DC细胞，观察组织细胞肉瘤变化。结果患者左乳肿块缩小＞50%，生活质量明显改善。结论细胞免疫治疗是一种化疗后组织细胞肉瘤有效的治疗手段，具有广阔的临床应用前景。
