AIM:To investigate intra-operator variability of semiquantitative perfusion parameters using dynamic contrast-enhanced ultrasonography(DCE-US),following bolus injections of SonoVue.METHODS:The in vitro experiments w...AIM:To investigate intra-operator variability of semiquantitative perfusion parameters using dynamic contrast-enhanced ultrasonography(DCE-US),following bolus injections of SonoVue.METHODS:The in vitro experiments were conducted using three in-house sets up based on pumping a fluid through a phantom placed in a water tank.In the in vivo experiments,B16F10 melanoma cells were xenografted to five nude mice.Both in vitro and in vivo,images were acquired following bolus injections of the ultrasound contrast agent SonoVue(Bracco,Milan,Italy) and using a Toshiba Aplio ultrasound scanner connected to a 2.9-5.8 MHz linear transducer(PZT,PLT 604AT probe)(Toshiba,Japan) allowing harmonic imaging("Vascular Recognition Imaging") involving linear raw data.A mathematical model based on the dye-dilution theory was developed by the Gustave Roussy Institute,Villejuif,France and used to evaluate seven perfusion parameters from time-intensity curves.Intra-operator variability analyses were based on determining perfusion parameter coefficients of variation(CV).RESULTS:In vitro,different volumes of SonoVue were tested with the three phantoms:intra-operator variability was found to range from 2.33% to 23.72%.In vivo,experiments were performed on tumor tissues and perfusion parameters exhibited values ranging from 1.48% to 29.97%.In addition,the area under the curve(AUC) and the area under the wash-out(AUWO) were two of the parameters of great interest since throughout in vitro and in vivo experiments their variability was lower than 15.79%.CONCLUSION:AUC and AUWO appear to be the most reliable parameters for assessing tumor perfusion using DCE-US as they exhibited the lowest CV values.展开更多
目的分析磁共振动态增强成像(DCE-MRI)定量灌注参数与原发性肝癌(PLC)患者病理分级及其预后的关系。方法回顾性分析收治的原发性肝癌106例患者的临床资料,并将其作为观察组,依据Edmondson-Steiner's肿瘤病理分级将观察组患者分为轻...目的分析磁共振动态增强成像(DCE-MRI)定量灌注参数与原发性肝癌(PLC)患者病理分级及其预后的关系。方法回顾性分析收治的原发性肝癌106例患者的临床资料,并将其作为观察组,依据Edmondson-Steiner's肿瘤病理分级将观察组患者分为轻度组(n=32例)、中度组(n=36例)和重度组(n=38例),随访2年,依据不同预后分为存活组(n=13例)和死亡组(n=93例);选择同期收治的肝脏良性结节患者52例作为对照组。所有患者均进行DCE-MRI检查,比较其定量灌注参数对比剂从血管到组织间的渗透率(Ktrans)、血管容积分数(Vp)、血管外细胞外间隙体积百分比(Ve)、血管外细胞外间隙转运至血管内的速率常数(Kep)变化,分析其在不同病理分级和不同预后患者的差异,以及定量灌注参数对原发性肝癌病理分级的效能。结果观察组患者DCM-MRI定量参数Ktrans、Vp、Ve和Kep均较对照组高(P<0.01)。重度组患者DCM-MRI定量参数Ktrans、Vp、Ve和Kep均较轻度组和中度组高,中度组上述参数较轻度组高,差异均有统计学意义(P<0.01);Spearman相关性结果显示,DCM-MRI各定量参数Ktrans、Vp、Ve和Kep与PLC病理分级均呈正相关(γ=0.522、0.501、0.513、0.574,P<0.05)。死亡组患者DCM-MRI各定量参数Ktrans、Vp、Ve和Kep均较存活组高,差异均有统计学意义(P<0.01)。受试者工作特征曲线(ROC)分析,DCM-MRI各定量参数Ktrans、Vp、Ve和Kep指导PLC轻度与中、重度的分级及预后评估均有较高效能,四者联合诊断时效能最高,其曲线下面积分别为(area under the curve,AUC)为0.841、0.893,约登指数为0.797、0.834,敏感度、特异性分别为92.31%、93.24%与86.67%、96.70%。结论DCM-MRI定量灌注参数与原发性肝癌患者病理分级密切相关,可以依据DCM-MRI定量灌注参数对患者进行病理分级及预后评估,并指导临床治疗。展开更多
Objective To investigate whether correlation existsbetween quantitative perfusion parameters obtained from dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)and different prognostic factors or immunohistoch...Objective To investigate whether correlation existsbetween quantitative perfusion parameters obtained from dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)and different prognostic factors or immunohistochemical subtypes of breast cancers.Methods A retrospective analysis of DCE-MRI was performed in展开更多
目的:探讨C T灌注成像在脑卒中患者侧支循环定量评价中的应用价值。方法:选取上海普陀区中心医院2019年4月—2020年4月收治的68例缺血性脑卒中患者,所有患者均接受常规C T平扫和C T灌注成像(C T P)检查。分析患者的脑血容量(CBV)、脑血...目的:探讨C T灌注成像在脑卒中患者侧支循环定量评价中的应用价值。方法:选取上海普陀区中心医院2019年4月—2020年4月收治的68例缺血性脑卒中患者,所有患者均接受常规C T平扫和C T灌注成像(C T P)检查。分析患者的脑血容量(CBV)、脑血流量(CBF)、达峰时间(TTP)和平均通过时间(MTT),分析侧支循环良好和不良的患者的C T P相关定量参数。结果:68例缺血性脑卒中患者中,有45例患者存在血管狭窄或闭塞。其中,侧支循环良好的21例,侧支循环不良的为24例。侧支循环良好患者C B V、C B F大于侧支循环不良患者,且梗死体积小于侧支循环不良患者。侧支循环良好患者的病灶侧CBF低于健侧,差异有统计学意义(P<0.05);平均通过时间,侧支循环良好患者病灶侧MTT、TTP长于健侧,差异有统计学意义(P<0.05)。侧支循环不良患者病灶侧CBV、CBF低于健侧,病灶侧M T T、T T P长于健侧,差异均有统计学意义(P<0.05)。结论:C T P检查可获取到患者的C T P相关参数,这些参数可评价侧支循环情况,继而帮助医师制定临床治疗方案,预测患者结局。展开更多
文摘AIM:To investigate intra-operator variability of semiquantitative perfusion parameters using dynamic contrast-enhanced ultrasonography(DCE-US),following bolus injections of SonoVue.METHODS:The in vitro experiments were conducted using three in-house sets up based on pumping a fluid through a phantom placed in a water tank.In the in vivo experiments,B16F10 melanoma cells were xenografted to five nude mice.Both in vitro and in vivo,images were acquired following bolus injections of the ultrasound contrast agent SonoVue(Bracco,Milan,Italy) and using a Toshiba Aplio ultrasound scanner connected to a 2.9-5.8 MHz linear transducer(PZT,PLT 604AT probe)(Toshiba,Japan) allowing harmonic imaging("Vascular Recognition Imaging") involving linear raw data.A mathematical model based on the dye-dilution theory was developed by the Gustave Roussy Institute,Villejuif,France and used to evaluate seven perfusion parameters from time-intensity curves.Intra-operator variability analyses were based on determining perfusion parameter coefficients of variation(CV).RESULTS:In vitro,different volumes of SonoVue were tested with the three phantoms:intra-operator variability was found to range from 2.33% to 23.72%.In vivo,experiments were performed on tumor tissues and perfusion parameters exhibited values ranging from 1.48% to 29.97%.In addition,the area under the curve(AUC) and the area under the wash-out(AUWO) were two of the parameters of great interest since throughout in vitro and in vivo experiments their variability was lower than 15.79%.CONCLUSION:AUC and AUWO appear to be the most reliable parameters for assessing tumor perfusion using DCE-US as they exhibited the lowest CV values.
文摘目的分析磁共振动态增强成像(DCE-MRI)定量灌注参数与原发性肝癌(PLC)患者病理分级及其预后的关系。方法回顾性分析收治的原发性肝癌106例患者的临床资料,并将其作为观察组,依据Edmondson-Steiner's肿瘤病理分级将观察组患者分为轻度组(n=32例)、中度组(n=36例)和重度组(n=38例),随访2年,依据不同预后分为存活组(n=13例)和死亡组(n=93例);选择同期收治的肝脏良性结节患者52例作为对照组。所有患者均进行DCE-MRI检查,比较其定量灌注参数对比剂从血管到组织间的渗透率(Ktrans)、血管容积分数(Vp)、血管外细胞外间隙体积百分比(Ve)、血管外细胞外间隙转运至血管内的速率常数(Kep)变化,分析其在不同病理分级和不同预后患者的差异,以及定量灌注参数对原发性肝癌病理分级的效能。结果观察组患者DCM-MRI定量参数Ktrans、Vp、Ve和Kep均较对照组高(P<0.01)。重度组患者DCM-MRI定量参数Ktrans、Vp、Ve和Kep均较轻度组和中度组高,中度组上述参数较轻度组高,差异均有统计学意义(P<0.01);Spearman相关性结果显示,DCM-MRI各定量参数Ktrans、Vp、Ve和Kep与PLC病理分级均呈正相关(γ=0.522、0.501、0.513、0.574,P<0.05)。死亡组患者DCM-MRI各定量参数Ktrans、Vp、Ve和Kep均较存活组高,差异均有统计学意义(P<0.01)。受试者工作特征曲线(ROC)分析,DCM-MRI各定量参数Ktrans、Vp、Ve和Kep指导PLC轻度与中、重度的分级及预后评估均有较高效能,四者联合诊断时效能最高,其曲线下面积分别为(area under the curve,AUC)为0.841、0.893,约登指数为0.797、0.834,敏感度、特异性分别为92.31%、93.24%与86.67%、96.70%。结论DCM-MRI定量灌注参数与原发性肝癌患者病理分级密切相关,可以依据DCM-MRI定量灌注参数对患者进行病理分级及预后评估,并指导临床治疗。
文摘Objective To investigate whether correlation existsbetween quantitative perfusion parameters obtained from dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)and different prognostic factors or immunohistochemical subtypes of breast cancers.Methods A retrospective analysis of DCE-MRI was performed in
文摘目的:探讨C T灌注成像在脑卒中患者侧支循环定量评价中的应用价值。方法:选取上海普陀区中心医院2019年4月—2020年4月收治的68例缺血性脑卒中患者,所有患者均接受常规C T平扫和C T灌注成像(C T P)检查。分析患者的脑血容量(CBV)、脑血流量(CBF)、达峰时间(TTP)和平均通过时间(MTT),分析侧支循环良好和不良的患者的C T P相关定量参数。结果:68例缺血性脑卒中患者中,有45例患者存在血管狭窄或闭塞。其中,侧支循环良好的21例,侧支循环不良的为24例。侧支循环良好患者C B V、C B F大于侧支循环不良患者,且梗死体积小于侧支循环不良患者。侧支循环良好患者的病灶侧CBF低于健侧,差异有统计学意义(P<0.05);平均通过时间,侧支循环良好患者病灶侧MTT、TTP长于健侧,差异有统计学意义(P<0.05)。侧支循环不良患者病灶侧CBV、CBF低于健侧,病灶侧M T T、T T P长于健侧,差异均有统计学意义(P<0.05)。结论:C T P检查可获取到患者的C T P相关参数,这些参数可评价侧支循环情况,继而帮助医师制定临床治疗方案,预测患者结局。
