Intelligent traffic system(ITS) is a development trend for urban modern traffic.Active guidance control for urban traffic can relieve increasing traffic congestion pressure effectively.FCD(floating car data) based dyn...Intelligent traffic system(ITS) is a development trend for urban modern traffic.Active guidance control for urban traffic can relieve increasing traffic congestion pressure effectively.FCD(floating car data) based dynamic guidance for urban traffic is one of the directions of traffic control research.To meet the real-time demand for massive FCD processing,an FCD parallel computing based dynamic traffic guidance approach is presented and implemented.The FCD properties are analyzed in detail.An FCD parallel computing based dynamic traffic guidance system is developed on KD-50-I-E platform.Experimental results demonstrate the effectiveness and feasibility of the proposed approach.展开更多
Prior to thixoextrusion, the microstructural evolution of semi-solid AM50A magnesium alloy prepared by the recrystallisation and partial remelting (RAP) route was investigated.The effect of compressive ratio on micros...Prior to thixoextrusion, the microstructural evolution of semi-solid AM50A magnesium alloy prepared by the recrystallisation and partial remelting (RAP) route was investigated.The effect of compressive ratio on microstructure of semi-solid AM50A magnesium alloy was studied.Furthermore, tensile properties of thixoextruded components were determined.The results showed that the occurrence of recrystallised grains was closely associated with the location of the first liquid formed above the solidus.With prolonging holding time, deformed microstructure was penetrated, causing fragmentation, which resulted in the decrease of average grain size.Moreover, prolonging holding time was favorable for the improvement of the degree of spheroidization due to the increase in the amount of liquid.With the increase in compressive ratio, the size of solid grain decreased, the degree of spheroidization was improved during partial remelting, and the tensile properties of thixoextruded AM50A components were improved.The tensile properties for AM50A magnesium alloy thixoextruded from starting material produced by the RAP route were better than those of the same alloy produced by die-casting and thixomolding.展开更多
Objective To explore the role of extracellular vesicles(EVs)in the pathogenesis of glaucoma caused by E50K mutation.Methods A photoreceptor cell line,RGC-5,was transfected with empty plasmids and plasmids expressing w...Objective To explore the role of extracellular vesicles(EVs)in the pathogenesis of glaucoma caused by E50K mutation.Methods A photoreceptor cell line,RGC-5,was transfected with empty plasmids and plasmids expressing wild-type(WT)optineurin(OPTN)or E50K OPTN to investigate the effects of OPTN glaucoma as well as to identify the role of EVs in glaucoma pathology.The RGC-5 cells were also stimulated with glutamate,and their viability was evaluated using flow cytometry or CCK-8 assay.EVs were extracted,labeled with PKH-26,and added into the medium for normal RGC-5 culture,and the status of the cells was observed thereafter.Results WT OPTN overexpression,E50K OPTN,and glutamate stimulation induced apoptosis of RGC-5 cells.However,when glutamate stimulation was used as an add-on treatment,the degree of apoptosis in WT OPTN-overexpressing RGC-5 cells was significantly lower than that in E50K OPTN-expressing and normal RGC-5 cells.The viability of normal RGC-5 cells was reduced when co-cultured with WT OPTN-overexpressing RGC-5 or E50K OPTN-overexpressing RGC-5.EVs released by the latter two transfected lines similarly reduced normal RGC-5 survival.Conclusion Our results indicate that WT OPTN overexpression may lead to photoreceptor apoptosis.However,overexpression also confers a degree of protection against high concentrations of extracellular glutamate.Additionally,EVs released by transfected RGC-5 cells may regulate the cell state.These findings may improve our understanding of the mechanisms of cell-cell interactions in pathological conditions,providing a basis for the use of EVs as novel targets for early diagnosis and treatment of glaucoma.展开更多
文摘Intelligent traffic system(ITS) is a development trend for urban modern traffic.Active guidance control for urban traffic can relieve increasing traffic congestion pressure effectively.FCD(floating car data) based dynamic guidance for urban traffic is one of the directions of traffic control research.To meet the real-time demand for massive FCD processing,an FCD parallel computing based dynamic traffic guidance approach is presented and implemented.The FCD properties are analyzed in detail.An FCD parallel computing based dynamic traffic guidance system is developed on KD-50-I-E platform.Experimental results demonstrate the effectiveness and feasibility of the proposed approach.
基金Project(51005217) supported by the National Natural Science Foundation of China
文摘Prior to thixoextrusion, the microstructural evolution of semi-solid AM50A magnesium alloy prepared by the recrystallisation and partial remelting (RAP) route was investigated.The effect of compressive ratio on microstructure of semi-solid AM50A magnesium alloy was studied.Furthermore, tensile properties of thixoextruded components were determined.The results showed that the occurrence of recrystallised grains was closely associated with the location of the first liquid formed above the solidus.With prolonging holding time, deformed microstructure was penetrated, causing fragmentation, which resulted in the decrease of average grain size.Moreover, prolonging holding time was favorable for the improvement of the degree of spheroidization due to the increase in the amount of liquid.With the increase in compressive ratio, the size of solid grain decreased, the degree of spheroidization was improved during partial remelting, and the tensile properties of thixoextruded AM50A components were improved.The tensile properties for AM50A magnesium alloy thixoextruded from starting material produced by the RAP route were better than those of the same alloy produced by die-casting and thixomolding.
基金supported by grants from the Wuhan Science and Technology Bureau(No.02.07.17040008.05)the Fundamental Research Funds for the Central Universities(2042020kf0099)the Opening Foundation of Hubei Key Laboratory(No.2021KFY054).
文摘Objective To explore the role of extracellular vesicles(EVs)in the pathogenesis of glaucoma caused by E50K mutation.Methods A photoreceptor cell line,RGC-5,was transfected with empty plasmids and plasmids expressing wild-type(WT)optineurin(OPTN)or E50K OPTN to investigate the effects of OPTN glaucoma as well as to identify the role of EVs in glaucoma pathology.The RGC-5 cells were also stimulated with glutamate,and their viability was evaluated using flow cytometry or CCK-8 assay.EVs were extracted,labeled with PKH-26,and added into the medium for normal RGC-5 culture,and the status of the cells was observed thereafter.Results WT OPTN overexpression,E50K OPTN,and glutamate stimulation induced apoptosis of RGC-5 cells.However,when glutamate stimulation was used as an add-on treatment,the degree of apoptosis in WT OPTN-overexpressing RGC-5 cells was significantly lower than that in E50K OPTN-expressing and normal RGC-5 cells.The viability of normal RGC-5 cells was reduced when co-cultured with WT OPTN-overexpressing RGC-5 or E50K OPTN-overexpressing RGC-5.EVs released by the latter two transfected lines similarly reduced normal RGC-5 survival.Conclusion Our results indicate that WT OPTN overexpression may lead to photoreceptor apoptosis.However,overexpression also confers a degree of protection against high concentrations of extracellular glutamate.Additionally,EVs released by transfected RGC-5 cells may regulate the cell state.These findings may improve our understanding of the mechanisms of cell-cell interactions in pathological conditions,providing a basis for the use of EVs as novel targets for early diagnosis and treatment of glaucoma.
